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Gram Stain
Material
solutions of crystal violet, Grams iodine (2 g potassium iodide in 300 ml distilled water plus 1 g iodine crystals), 95% ethanol and/or isopropanol-acetone mixture (3:1 v/v), and safranin Bismark brown stain (for color-blind students) clean glass slides inoculating loop Bunsen burner bibulous paper
microscope lens paper and lens cleaner immersion oil Hyphomonas (Hyphomicrobium) neptunium (ATCC 15444) grown in marine broth (Difco) slide warmer staining rack Bacto Gram Stain Reagents from Difco for the three-step Gram stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain
Gram Stain