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DEFINITIONS IN HAEMATOLOGY
Mean corpuscular Haematocrit
= N = 80 – 96 μ
volume (MCV) Red cells count
3. Specific signs:
– koilonychia – spoon-shape nails seen in iron deficiency
anaemia
– jaundice – haemolytic anaemia
– bone deformities – thalassemia major
– leg ulcers – sickle cell disease
Classification:
2. Hypochromic microcytic with low mean corpuscular volume
(MCV)
3. Normochromic normocytic with a normal MCV
4. Macrocytic with a high MCV
Special investigations:
– bone marrow aspiration from the sternum or posterior illiac
crest is performed to:
– confirm a diagnostic made from peripheral blood count
– determine the cellularity of the marrow
– determine the type of erythropoiesis
– determine the proportion of the various lines
– see wether the marrow is unfiltrated
– determine the size of the iron stores
MYCROCYTIC ANAEMIA
}
– small cells (microcytes)
Thalassaemia
– low MCV (< 80 μL)
– normal iron content hyperplastic Sideroblastic anaemia
1.IRON DEFICIENCY
– brittle nails
– spoon – shaped nails (koilonychia)
– atrophy of the papillae of the tongue
– angular stomatitis
– brittle hair
– dysphagia and glossitis (plummer – Vinson or Paterson Brown
Kelly syndrome)
– parotid gland enlargement, splenomegaly and failure to grow
investigations:
– the red cells are microcytic (MCV < 80 fL) and hypochromic
(MCV < 27 pg)
– poikilocytosis (variation in shape) and anisocytosis (variation in
size)
– target cells
– hypersegmentation of polymorphs
– serum iron falls
– iron blinding capacity ↑
– bone marrow – erythroid hyperplasia with ragged normoblasts
– ring sideroblast
other investigations:
– the G.I. tract - endoscopy
Bone marrow in iron deficiency
1.sideroblastic anaemia
Classification:
A. Congenital:
– “X” linked disease – transmitted by females
B. Acquired:
– primary or idiopathic
– secondary:
drugs
alcohol
lead
myeloproliferative disorders
leukaemias
secondary carcinoma
other systemic disorders (connective tissue
disease)
1.thalassaemia
Deficiency in the synthesis of the globin chains of haemoglobin
in addition, the accumulation of abnormal chains within the red
cell leads to its early destruction.
– failure to thrive
– intermittent infection
– severe anaemia
– extramedullary haemopoiesis → hepatosplenomegaly and bone
expansion thalassaemic facies
investigation:
– blood count:
moderate to severe anaemia (↓MCV, MCH↓)
reticulocyte ↑
white cells and platelets = N
– blood film:
hypochromic and microcytic picture
Howell – Jolly bodies
– high ferritin levels
– haemoglobin electrophoresis (HbF ↑; HbA absent)
β-Thalassaemia trait (minor)
– asymptomatic
– no anaemia, red cells hypochromic and microcytic
α-Thalassaemia
– two main form:
deletion of only alpha chain gene
deletion of both alpha chain genes → no alpha chains are
produced
Thalassemia major
Thalassemia minor
Macrocytic anaemia
The presence in the bone marrow of erytroblasts with delayed
nuclear maturation because of defective DNA synthesis
(megaloblasts).
Occurs in:
–vitamin B12 deficiency
–folic acid deficiency
–diseritropoetic anaemia
Haematological values:
–anaemia
–MCV > 96 fL
–blood film (peripheral): macrocytes and hypersegmented
polymorphs
–neutropenia
–thrombocytopenia
vitamin b12 (Addison – Biermer anaemia)
– average daily diet 5 – 30 μg B12
–average adult stores 1000 μg – liver
–absorption and transport:
gut → binder complex (R binder + B12) → intrinsec
factor (glycoprotein from the gastric juice)
Transcobalamin
Ileum → → → → → → → → → → Marrow
Pernicious anaemia (Addison – Biermer) affect:
– particularly nordic people: fair – haired; blue – eyed.
–association with other autoimmune diseases: thyroid disease,
Addison’s disease, vitiligo
– higher incidence of gastric carcinoma
Causes of vitamin b12 deficiency:
– low dietary intake (vegans)
– impaired absorption:
A. stomach (gastrectomy)
B. small bowel:
– coeliac disease
– tropical sprue
– bacterial overgrowth
– ileal disease or resection
C. pancreas:
– chronic pancreatic disease
– Zollinger – Ellison syndrome
D. miscellaneous and rare:
– fish tape worm (diphyllobothrium latum)
– congenital deficiency:
– intrinsec factor
– transcobalamin III
– nitrous oxide (inactivates B12)
Clinical features:
2. Anaemic syndrome
3. Neurological syndromes:
Peripheral neuropathy progressively involving the
posterior and lateral columns of the spinal cord:
– symmetrical paraesthesia in the fingers and toes
– loss of vibration sense and proprioception
– progressive weakness and ataxia
– paraplegia
Mental changes:
– somnolence
– irritability
– psychosis
– dementia
1. Digestive syndrome:
– glossitis (red sore tongue)
– angular stomatitis
– hepatosplenomegaly
– gastric atrophy and achlorhydria
2. Others:
– skin – lemon-yellow tint due to hyperbilirubinaemia
– heart – failure
– fever
Investigations:
Stage II (2 – 4 days):
– Hb, Ht, Rc ↓
– reticulocytosis
– ↓ white cells
Stage III (2 – 3 weeks):
– ↓ platelets
–Hb, Ht, Rc
–Wc
–Platelets
} N
1.Aplastic anaemia
Aplasia of the bone marrow with peripheral blood pancytopenia.
Causes:
– congenital: Fanconi’s anaemia
– acquired:
– chemicals, drugs, insecticides
– ionizing radiation
– infections: viral hepatitis measles
– miscellaneous infection: tuberculosis
– tyhmona
– pregnancy
– unknown
clinical features:
– anaemia
– bleeding (ecchymoses, bleeding gums and epistaxis)
– infection (fungal infections)
investigations:
– elevated serum iron
– low haemoglobin
– white cell
– count 500 / mmc
– platelet 20,000 / mmc
– reticulocytes virtual absent
– hypocellular or aplastic bone marrow
1.Haemolytic anaemia
The red cells normally survives about 120 days, but in
haemolysis the cell survival times are considerably shortened.
Causes of haemolytic anaemia:
D. Inherited:
1. red cell membrane defect:
– hereditary spherocytosis
– hereditary eliptocytosis
2. haemoglobin abnormalities:
– thalassaemia
– sickle cell disease
3. metabolic defects:
– glucose 6 phosphate dehydrogenize deficiency
– pyruvate kinase deficiency
(Causes of haemolytic anaemia)
B. Acquired:
1. immune:
– autoimmune
– isoimmune (Rh or ABO incompatibility)
2. non-immune:
– membrane defects: paroxysmal nocturnal
haemoglobinuria, liver disease, renal disease
– mechanical: damaged vessels, valve prosthesis, march
haemoglobinuria
3. miscellaneous:
– infections
– drugs and chemicals
– hypersplenism
site of haemolysis:
Causes of polycythaemia
Primary:
– Polycythaemia vera
Secondary:
A. due to an appropriate increase in erythropoetin:
– high altitude
– lung disease
– cardiovascular disease (right left shunt)
– heavy smoking
B. due to an inappropriate increase in erithropoetin:
– renal disease, carcinoma, Wilms tumor
– hepatocellular carcinoma
– adrenal tumors
– cerebellar haemangioblastoma
– massive uterine fibroma
Relative:
– stress or spurious polycythaemia
– dehydration
– burns
Policitemia vera
Caused by chronic sustained proliferation of the erithroid
population of the bone marrow.
↑ blood viscosity
Ht ↑ ↑ ↑
compensated by an
increase plasma
volume and myocardial infarction
cardiac output stroke
clinical findings:
tiredness
depression
vertigo
tinitus and visual disturbance
hypertension
angina
intermitent claudication
tendency to bleed
itching after bath
peptic ulcerations
investigations:
Neutrophil luecocytosis
rise in the number of neutrophils to > 10x105/l in bacterial
infection or tissue damage
exercise
corticosteroid administration
Neutrophil leucocytosis
leukaemia
myeloproliferative disease
leukaemoid reaction
leucoerytroblastic anaemia
the leucocytosis may be accompanied by a pyrexia due to
the production of a leucocyte pyrogen
a leukaemoid reaction (the overproduction of white cells,
many of them primitive) may occur in - severe infections
- tuberculosis
-malignant infiltration
neutrophilis
Neutropenia and agranulocytosis
defined as a circulatory neutrophil count below 1,5x109/l
the absence of heutrophilis is called agranulocytosis
causes of neutropenia
rasial (neutropenia is common in black rases)
viral infection
severe bacterial infection (typhoid)
Felty’s syndrome
megaloblastic anaemia
drugs
pancytopenia from any cause
Clinical features:
infections
glazed mucositis occurs in the mouth and ulceeration is
common
septicaemia
investigation
blood film shows neutropenia
bone marrow – absence of cells from the neutrophil
granulocyte series
eosinophils
– Occur when the number of eosinophils is > 1x109/l
causes of eosinophils
Parasitic infestation
– ascaris
– strongyloides
Allergic disorders
– hayfever (allergic rhinitis)
– other hypersensitivity reactions, including drug reactions
Skin disorders
– urticaria
– eczema
– pemphigus
Pulmonary disorders
– bronchial asthma
– tropical pulmonary eosinophilia
– allergic bronchopulmonary aspergillosis
– polyarteritis nodosa (Churg – Strauss syndrome)
Malignant disorders
– lymphoma
– carcinoma
– melanoma
– eosinophilic leukemia
Miscellaneous
– sarcoidosis
– hypoadrenalism
– eosinophilic gastroenteritis
– hypereosinophilic syndrome
lymphocytes
• Form nearly the circulating white cells
• Originate in the lymph glands, spleen, Peyer’s patches, bone
marrow, thymus
2 types:
thymus dependent or T lymphocytes concerned with
cellular immunity
“bursa – dependent” or B lymphocytes concerned with
humoral immunity
clasification
There are TWO MAJOR of acute leukemia:
F. Acute lymphoblastic leukaemia
G. Acute non-lymphocytic leukaemia (called also acute
myelogenous leukaemia)
The CHRONIC FORMS of these conditions are:
Chronic granulocytic leukaemia
Chronic lymphatic leukaemia
incidence
– the commonest childhood leukaemia is acute lymphoblastic in
type (80%)
– adults B and in elderly – chronic forms
aetiology
– remains unknown
Genetic factors:
– are important: low frequency of all in black children
– a high incidence of leukaemia in the identical twin
– ↑ risk of developing acute leukaemia in children with Down’s
syndrome (who have chromosomal abnormalities)
Enviromental factors:
radiation (in survivors of the atomic bomb of Hiroshima)
chemicals
drugs and chemotherapeutic agents
viruses (human leukaemia virus type I) which was first
discovered in Japanese with T cell leukaemia and
hypercalcaemia
Acute leukaemia
Cellular types
3. Acute lymphoblastic leukaemia
– blast cells involved may vary
– histologically: L1, L2 and L3 types
– the phenotypic markers have proved to be of considerable
importance assessing the likelihood importance of
response and the long-term outlook
4. Acute non-lymphocytic leukaemia
classification
Acute myelocytic • predominant myeloblasts, distinct
M1 leukaemia without nucleoli
differentiation• few granules Auer rods – rare
Acute myelocytic • myeloblasts and promyelocytes
Leukaemia with predominant
M2 Differentiation • further maturation abnormal
• auer rods – many
Acute • promyelocytes predominate
M3 promyelocytic hipergranular
leukaemia • auer rods – rare
Acute • myelocytic and monocytic maturation
M4 myelomonocytic evident may be peripheral
leukaemia • auer rods – rare
Acute monocytic • promonocytes predominant with
M5 leukaemia differentiation
Acute monoblastic • completely with differentiation
M5 leukaemia • undifferentiated blast cells
A
• bizzare, multinucleated megaloblasted
M6 Erytroleukaemia erythroblasts predominate
• myeloblasts also present
Acute leukaemia
clinical features
Hystory short
symptoms of anaemia and maladive
acute infections such as mouth ulceration, sore throat,
pneumonia, perianal and skin infections
painful and enlarging lymphadenopathy
bruising and bleeding
bone pain (particularly common in children with all)
symptoms due to infiltration of tissues with leukaemic blast
cells, marked gum hypertrophy
headache, nausea, vomiting and blurred vision (raised
intracranial pressure)
Signs
These may be relatively few, but commonly they are:
pallor
bruising, petechial haemorrages, bleeding gums and gum
hypertrophy
lymphadenopathy
splenomegaly and hepatomegaly
haemorrhages in the optic fundi with characteristic central
white deposit in the middle of the fundal haemorrhage →
leukaemic retinopathy
meningeal leukaemia
boys – hard enlarged testicles (infiltrated with leukaemic
tissue)
investigation
2. Peripheral blood film and bone marrow
– normochromic and normocytic anaemia
– the white cell count may be normal or raised; rarely a few
blast cells may be seen in the peripheral blood, or none at
all
– the platelet count is usually reduced
– hypercellular bone marrow with characteristic blasts in the
trail of the fragments on the microscope slide
3. The CSF should be examined – will contain blasts cells if
meningeal leukaemia is present
4. Test of renal function
5. Serum uric acid
6. Serum calcium
7. Serum electrolytes (potassium)
8. Blood cultures
9. Chest X ray (to determine the presence of a mediastinal mass)
Gum-hypertrophy ALL
Blasts-and-Auer-body
ALL
ALL Blast
ALL-L1-Marrow
Chronic granulocytic leukaemia
– occurs in middle-aged and elderly people
– it occurs in the myeloproliferative syndromes, which include:
polycythaemia vera, myelofibrosis, essential trombocytosis
– it is characterised by the presence of Philadelphia
chromosome
Clinical features
often of insidious onset (may only be discovered on a routine
blood count)
anaemia
bruising and bleeding manifestations
pain or discomfort due to a very large spleen →
gastrointestinal disturbance
sweating, fever and loss of weight as the result of a high
metabolic rate
Phisical signs
anaemia
lymphadenopathy (uncommon)
a large spleen (common)
haemorrhage and thrombosis; bruising, bleeding, priapism
may occur
gout
Investigations
clinical features
• the onset is insidous
• lethargy
• fever and sweating
• loss of weight
signs
moderate enlargement of lymph nodes in the neck, axilla and
groin
splenic and hepatic enlargement, but not usually massive
investigations
mild anaemia, normochromic, normocytic
white cell count > 15x109 &l, which more than 40%
lymphocytes
platelet count is usually normal as the disease progresses,
anaemia may become severe due to Coombs positive
haemolysis and the number of lymphocytes ↑