EQUIPMENT USE IN MOLECULAR PATHOLOGY SECTION

Presented by Nazneen Islam

Why we are looking for Automation?

DISEASE DIAGNOSIS

TIME SAVING
REDUCE MAN POWER ACCURACY

THOROUGH PUT

EQUIPMENT USE IN MOLECULAR PATHOLOGY SECTION

High speed centrifuge PCR Machine Pipettes

Heat block
Nano Drop Vortex Picofuge Biosafety cabinet

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Elisa plate reader Cobas Amplicor Cobas Ampliprep/Cobas TaqMan 96 and 48 Gel electrophoresis Gel documentation system

PH meter
-80 & -20 freezers Electrical balance

CEQ 8000 Analyzer

PH Meter
PH is measure of the acidity or basicity of a solution
Ranges 0-14.

Principle

When PH meter electrode placed in a unknown solution an electrical potential difference produced due to difference of H+ ion conc. b/w the test solution and the electrode & reading is produced 0-14.

High Speed Centrifuge

Help for the separation of WBCs from the blood for DNA/RNA extraction Principle based on sedimentation

A centrifuge uses centrifugal force to isolate suspended particles from solution.

Thermal Cycler
PCR is a technique use to amplify target DNA/RNA &

generating thousands or millions of copies of a particular DNA sequence.

The method relies on thermal cycling consisting of

cycles of repeated heating and cooling of the reaction.

Main tool for clinical diagnosis of viruses/

bacteria/genetic disease

Thermal Cycler

Real Time PCR

Highly sensitive High resolution Automated Results expressed in numbers Post PCR processing not required Accurate results

Agarose Gel Electrophoresis

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Pipettes & Tips

Weighing Balance
Balance shows the weight of a substance digitally & is used to weigh chemical in labs. Use for weighing the smallest quantity of 0.001 to 1 kg of chemical , Enzymes and other reagents

Magnetic Stirrer
Use to prepare the reagent Stirrer the magnetic bar in

solution and dissolve the solute particle Where as hot plate use for mixing the unsaturated solute particle into the solvent

Vortex Mixer
Mixes the molecular biochemical reactant and help to provide the homogeneous mixture

Biosafety Cabinet
The biological safety cabinet (BSC) is the principal device used to provide containment of infectious splashes or aerosols generated by many microbiological procedures.
Protect technologist Protect sample Isolate sample from

contamination Keep lab environment clean

Water Bath
Help during DNA extraction Provide optimum temp for PK

digestion Help to readily mix DNA in water STUART Shaking Water Bath Several incubation for genotyping of viruses

Heat Block
Incubate sample for

RNA/ DNA extraction. During PK/SDS Digestion. Help in mixing of high Conc. of DNA into water quickly.

Genetic Analyzer

Sequence the DNA Detect mutation Detect different diseases Pre & post donor Chimerism

Genetic Analyzer

Principle of Genetic Analyzer:

The fragments migrate through the gel according to size. The laser beam excites the dye attached to each fragment, causing the dye to emit fluorescence that is captured by the detector. The detector converts the fluorescence to an electrical signal that is recognized by computer software as a flash or peak of color. Fluorescent detection equipment yields results as an electropherogram,

Cobas Ampliprep

Cobas Amplicor

The COBAS AMPLICOR Analyzer offers: Bar-coded data entry 48 sample capacity per run Test-specific reagents Perform PCR and goes through the automated walk away probe based detection

Abbott m 2000 SP/RT

THANK YOU