Sei sulla pagina 1di 9

Growth Patterns in Broth

1. ring or pellicle growth on surface 2. flocculent (clumps) 3. uniform turbidity 4. sediment

Colony morphology
1. Colony shape and size: round, irregular, punctiform (tiny) 2. Margin (edge): entire (smooth), undulate (wavy), lobate (lobed) 3. Elevation: convex, umbonate, flat, raised 4. Color: color or pigment, plus opaque, translucent, shiny or dull 5. Texture: moist, mucoid, dry (or rough).

Bacillus

Proteus

Staphylococcus

Streptococcus

Motility agar stab agar with wire, not loop

Mannitol Salt Agar


Purpose Mannitol salt agar is both a selective and differential growth medium. It is used to differentiate pathogenic Staphylococcus species from nonpathogenic members of the genus Micrococcus. Principle Mannitol salt agar can help determine two characteristics of bacteria, whether they are salt tolerant or not, and whether they are able to ferment mannitol. Salt Tolerance (Does it grow?). This medium contains 7.5% salt and therefore "selects" for organisms that are able to tolerate the presence of high levels of salt.If the organism grows, it is salt tolerant. If the organism does not grow, it is not salt tolerant. Therefore, we say that MSA is "selective," as it will "select" for salt-tolerant organism.

MSA continued salt/mannitol fermentation


1. S. aureus +/- (yellow)
2. S. epidermidis +/3. M. luteus -/na

MacConkey Agar
Purpose MacConkey agar is a widely-used culture medium which is both selective AND differential. The medium is primarily used to differentiate between Gram negative bacteria while inhibiting the growth of most Gram positive bacteria. The medium also differentiates between lactose-fermenting coliforms and lactose nonfermenters, which include potential pathogens. Principle Addition to the nutrient agar base of bile salts and crystal violet will inhibit the growth of most Gram positive bacteria, making MacConkey agar selective. Lactose, a fermentable carbohydrate, and neutral red, a pH indicator, are added to differentiate the lactose positive coliforms from the potentially pathogenic lactose nonfermenters. Additional Information When lactose is fermented, acid products lower the pH below 6.8, with the resulting colonial growth turning pinkish-red. If an organism is unable to ferment lactose, the colonies will be colorless.

Mac continued
Staphylococcus aureus

Enterobacter cloacae on MacConkey Agar: growth with pink colonies

Eschericia coli on MacConkey Agar: growth, with pink colonies

Potrebbero piacerti anche