Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
SUDJADI
Pengampu
Sarmoko, S.Farm., Apt. 0812 1531 3748 sarmoko@gmail.com Iskandar Sobri, S.Farm., Apt.
PUSTAKA
BR Glick & JJ Pasternak, Molecular Biotechnology DJA Crommelin & Rd Sindelar, Pharmaceutical
Biotechnology JM Tabor, Genetik Engineering In Industry Pharmacy Sudjadi, Bioteknologi Kesehatan Walsh, G., 2007, Pharmaceutical Biotechnology,
John Wiley and Sons Walsh, G., 2003, Biopharmaceuticals: Biochemistry and Biotechnology, John Wiley and Sons
Biotechnology Today
Medical Biotechnology Medical Research Disease Identification Genetic Screening Designer Drugs/Vaccines Gene Therapy Tissue/Organ Engineering Forensics DNA Fingerprinting
$450 Billion dollars in annual revenues with projected annual growth of 10-15%. The US accounts for ~70% of the Global Biotech Industry. Biomanufacturing jobs are among the highest paying in the manufacturing sector. Starting wages of $25,000-$30,000 per year that can grow to $40,000-$50,000 with experience.
Bioteknologi
Ilmu yang mempelajari penggunaan agen hayati untuk menghasilkan barang Ilmu yang mempelajari penggunaan agen hayati/bagiannya untuk menghasilkan barang/jasa
Bioteknologi Klasik
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$ 35
menjadi 71.25
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1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38.
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1981 1990
1. Meningitis vaccine 2. Hepatitis B vaccine 3. Heberkinasa 4. Heberon alfa r 5. Hebermin 6. Gavac 7. SUMA System 8. DIRAMIC 9. Hebertrans 10.Culture media 11.Policosanol 12.Trofin 13.Natural products 14.Neurodiagnostic systems 15.Anti-CD3 monoclonal antibody 16.Surfacen 17.Generics 18.Placenta derivatives 19.Neurological restoration services
1991 2000
Haemophylus B vaccine Combined vaccines (HB-Hib,DPT-Hib, DPT-HBb-Hib,). Vaccines and MAbs for cancer therapy Meningitis vaccine Hepatitis B vaccine Meningococcus ACYW135 vaccine HB Uniject vaccine EPO (CIM, CIGB) Products and equipment for Neurophysioloy and Neuroinformatics New diagnostic systems Streptokinase (w/o HSA) Neurological restoration services Leptospirae vaccine MEN B vaccine Salmonella vaccine Tethanus Toxoid GCSF Allergens Trofin Interferon (liquid, w/o HSA) Interferon (liofylized, w/o HSA) Interferon + ribavirine Gamma Interferon Interleukin-2 PPG-plus Humanized anti EGF-receptor antibody SUMA system Agriculture products (GAVAC, Bionematicide) EGF (parenteral) Hebertrans Culture media New advanced generics Cytostatics Technology transfers Placenta derivatives EPO plus Surfacen 12 EGF viscous solution
2001 2005
Pharmaceutical
Antibiotics (Penicillin, Streptomycin, Semisynthetic Antibiotics) Vitamins (Fermentation to produce Vitamin C) Biological Medicines (Interferon, Interleukin, TNF, HGH, CSF, Calcitonin, etc.) Diagnostic Reagents (ELISA, PCR, etc) Health Protection Products ( Melatonin, NeuroPeptide, Nuclear Acids)
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Status: Imported and Assembly Many company produced the same product (In year 2000 200 companies and 15 medicines get License) Examples:
*Recombinant *Recombinant *Recombinant *Recombinant (GM-CSF) *Recombinant *Recombinant *Recombinant *Recombinant Human Human Human Human Human Human Human Human Interferon (1a, 2a, 2b) Interliukin (IL) Colony Stimulating Factor (CSF) Granulocyte-Macrophage Colony Stimulating Factor Epidermal Growth Factor (HEG) Growth Hormone (HGH) Basic Fibroblast Growth Factor Erythrogenin (EPO)
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ELISA (HBV, HCV, HIV, etc) PCR Kit Colloid Gold Labeling DNA Chip and Protein Chip
*Antibody Protein Chip Diagnostic System *Pylorus Spirus Bacillus Antibody Protein Diagnostic System *Multiple Cancer Labeling Protein Chip Diagnostic System
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Forensic
*Criminal investigation *Paternity testing *Body identification for Victims of Accident
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Four Lessons
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Entrepreneurship is Key
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Conclusions
Place still matters, even in a quintessential knowledge industry Not just research, but the ability to turn ideas into businesses The power of clustering provides decisive business advantages
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BIOTEKNOLOGI MODERN
Menggunakan agensia hayati/bagian darinya Menghasilkan barang/jasa Integrasi kaidah ilmiah dan perekayasaan Sampai aras molekuler
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Ruang-lingkup
Rekayasa genetik Teknik biologi molekular : PCR, ELISA Diagnosis cepat dan tepat: peny. infeksi dan peny genetik Produksi antibiotik, enzim, hormon, asam amino Tanaman/hewan transgenik yang menghasilkan obat Terapi sel punca
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Ruang lingkup
Human genetics (Human Genome Project) Sidikjari DNA Terapi gena Antisense Vaksin
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Kekhawatiran
Prosedur diagnosis melanggar HAM ? Pengobatan dengan teknik molekuler utk orang kaya
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Human 2.9x109 bp
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(Berg JM et al, 2002)
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Strand baru
Topoisomerase DNA
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ACYCLOVIR GANCYCLOVIR
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REAKSI PERPANJANGAN
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BPOM PROPOSAL
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PCR
Gen Normal
Gen Penderita
Elektroforesis
Normal Pembawa Sakit
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Splising
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DNA vektor
EcoRI
Ligase
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Membran nilon
Hibridisasi
protein
X
antibodi
(Berg JM, et al, 2002)
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James Thomson, Ph.D in developmental biology, successfully cultured immortal, human embryonic stem cells in 1997. Culmination of 17 years of research. Science 282: 1145-1147 (1998) 1999 Science Magazine Breakthrough of the Year. Thomson on the cover of Time Magazine as one of the top scientists in the U.S.; numerous other news stories. Two U.S. patents
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Stem Cell = a cell which will reproduce itself and is also capable of giving rise to a more specialized cell. HES Cell = derived from the inner mass cells of an embryo, is pluripotent i.e. capable of giving rise to any cell type in the body; and is immortal i.e. continued, indefinite, replication without differentiation under proper culture conditions.
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