BTB 1xx Basic Cell Biology (1+1)

2007 syllabus 2011 syllabus

Cell Biology

(2+1)

Basic Cell Biology (1+1)

Applied Cell Biology

(2+0)

BTB 1xx

Basic Cell Biology (1+1)

Aim: To impart basic knowledge on evolution of cell, microscopy, cell structure, organelles, cell cycle and embryology

Theory
Unit I Evolution of cell and Microscopy History of cell science and techniques, Origin of life, Hierarchy in life- Process of evolution Fundamentals, types and applications of microscopy in cell biology. Unit II Cell structure Structure of prokaryotic and eukaryotic cellskinds of eukaryotic cells modifications, cell wall, extracellular matrix, biomembrane system and cytoskeleton network

Theory (contd.)
Unit III Cell organelles Ultra structure and functions of mitochondria, plastids, nucleus, ribosomes, endoplastic reticulum, Golgi bodies and lysosomes Unit IV Cell cycle Cell cycle Interphase: G1, S and G2, M phase: mitosis and cytokinesis, cell cycle control and programmed cell death Unit V Basics in embryology Spermatogenesis, oogenesis and fertilizationcleavage development - organogenesis

Practicals
Unit I Microscopy and prokaryotic cells Study of light microscopes, staining procedure, microscopic examination of prokaryotic cells Unit II Quantitation and Micrometry Quantitation of cells using Haemocytometer, Cellular micrometry Unit III Eukaryotic cells and organelles Microscopic preparation of plant and animal tissues, isolation of mitochondria and chloroplast from plant cell

Practicals (contd.)
Unit IV Cell cycle Identification of mitotic stages and meiotic stages. Unit V Cell culture Study of cell growth parameters.-Biological application-Plant cell culture. Practical Examination.

Lecture Schedule
1. History of cell science and techniques 2. Origin of life and hierarchy in life 3. Process of evolution from progenitor cells to eukaryotes 4. Fundamentals, types and applications of microscopy in cell biology 5. Structure of basic cell and special features of prokaryotes, plant and animal cells 6. Different kinds of cells and and their modifications in plants and animals 7. Structure and function of plant cell wall 8. Biomembrane system 9. Mid semester examination

Lecture Schedule (contd.)

10. Cytoskeleton network in cell and extracellular matrix 11. Ultra structure and function of mitochondria 12. Ultra structure and functions of plastids in plants, modification in structure and function of photosynthetic cells 13. Ultra structure and functions of nucleus in cells 14. Cell cycle interphase and M phase 15. Cell cycle control and programmed cell death 16. Spermatogenesis, oogenesis and fertilization 17. Clevage- development and organogenesis

Practical Schedule
1. Study of light microscopes 2. Microscopic examination of various types of prokaryotic cells 3. Preparation of fixatives 4. Study of staining procedures 5. Staining of prokaryotic cells 6. Quantitation of cells using haemocytometer 7. Cellular micrometry 8. Microscopic preparations of plant tissues monocots

Practical Schedule (contd.)

9. Microscopic preparations of plant tissues dicots 10. Microscopic preparations of animal tissues 11. Identification of mitotic stages using onion root tips 12. Identification of meiotic stages 13. Isolation of mitochondria 14. Isolation of chloroplast 15. Study of cell growth parameters 16. Biological application-Plant cell culture. 17. Practical Examination.

Text Books
Verma, P.S. and Agarwal, V.K. 1994. Cytology. S.Chand & Co., New Delhi. Lodish, H., Matthew P., Scott. P. M., Darnell, J. L., Chris, Z. Monty, K. 2003. Molecular Cell Biology 5th Edition, W. H. Freeman Publishers. Alberts, B, Alexander, J, Lewis, J, Marti. R, Keith. R and Walter, P. Molecular Biology of the Cell, Fourth Edition, 2002. Garland Publishers.

Further reading
Darnell, J., Lodish, H. and Baltimore, D. 1990. Molecular Cell Biology, Freeman W.H., Scientific American Books Inc. Gunning, B.E.S and Steer, M.W. 1996. Plant Cell Biology, Jones and Bartlett Publishers. London. Garret, R.H. and Grisham C.M.. 1995. Molecular aspects of Cell Biology. Part V. Saunders College Publishing, New York Sheeler, P. and Bianchi, D.E. 1987. Cell and Molecular Biology. John Wiley & Sons. Inc. New York. Wilson, J. 2002. Molecular Biology of the Cell: A Problems Approach 4th Edition. Garland Publishers.

Online references
Dictionary of Cell Biology Virtual Cell http://www.life.uiuc.edu/plantbio/cell/ http://www.uct.ac.za/depts/mmi/stannard/e mimages.html 3-D Interactive Cell http://www.talksaver.com/indexes/cellbio.h tm Online cell biology course http://ccgb.umn.edu/~mwd/cell.html

Outcome of the course

The students will know about the basic aspects of structure of the cell, cell organelles, its functions cell cycle and embryogenesis