Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
DIRECT IDENTIFICATION
CPS ID 3, NEW generation contains specific substrates of the enzymatic activities to be detected.
GREATER PRATICABILITY
E.coli: -glucuronidase (-GUR). No indole test required. Proteus: spontaneous colouration of colonies producing deaminase. No additional TDA test required. Enterococci: -glucosidase (-GLU).
Turquoise colonies
-glucuronidase -glucosidase +
Proteus E. coli
Detection of Indole
Indole + Indole -
Bacilli
Proteus mirabilis
Enterococci
Follow up identification
Nutrient capacity, mainly for staphylococci and yeasts. Sensitivity of detection and specificity of colouration for the main organisms usually found in urine. Identification of the bacterial group KESC (Klebsiella, Enterobacter, Serratia and Citrobacter) KESC => better colour intensity, due to increased -glucosidase activity. Orientation of identification towards Streptococcus agalactiae.
Klebsiella pneumoniae
Green to brownish-green colonies : -GLU + Direct examination : bacilli Complete the identification
Pseudomonas aeruginosa
Pigmented colonies Direct examination : mobile bacilli Oxidase + Complete the identification
Staphylococcus aureus
Immediate identification with : Usually yellow colonies Direct examination : cocci Catalase + Slidex Staph Plus
Mixed culture showing : E.coli and Enteroccoci Mixed culture showing : E.coli, Proteus and Enteroccoci
Immediate identification with :
Turquoise : Enterococci
Mixed culture showing : K.pneumoniae and Enteroccoci Mixed culture showing : E.coli and P. mirabilis
Microbial enumeration :
Inoculation with a 10 l calibrated loop. Interpretation of bacteriuria : < 104 CFU/ml : probable contamination between 104 and 105 CFU/ml : equivocal result, repeat test on a new specimen > 105 CFU/ml : probable infection Compare the density of the colonies detected with that illustrated below (CFU = Colony forming Unit) :
105 CFU/ml
106 CFU/ml
12-03 / 002GB99072A / This document is not legally binding. bioMrieux reserves the right to modify specifications without notice BIOMERIEUX, the blue logo and Slidex are registered and protected trademarks belonging to bioMrieux sa or one of its subsidiaries Printed in France / THERA McCann Healthcare / RCS Lyon B 398 160 242
CPS ID 3 : Ref. 43 541 : kit of 20 plates - Ref. 43 549 : kit of 100 plates ID Indole TDA : Ref. 56 541 : 200 tests ID color Catalase : Ref. 55 561 : 100 tests Oxidase Reagent : Ref. 55 635 : 50 ampules RAPIDEC STAPH : Ref. 03 300 : 10 strips x 3 tests Slidex Staph Plus : Ref. 73 115 : kit of 50 tests - Ref. 73 116 : kit of 250 tests bioMrieux offers a wide choice of media, including a comprehensive range of ready-to-use chromogenic media. Consult your local bioMrieux representative for further information and availability.
* For more details see technical sheet
bioMrieux sa 69280 Marcy lEtoile France Tel. : (33) 04 78 87 20 00 Fax : (33) 04 78 87 20 90 www.biomerieux.com