Chapter 1 The Problem and the Setting Introduction Once established, a root canal infection is difficult be eliminated by the

host defence mechanisms or by systemic antibiotic therapy, as absence of blood supply in a necrotic pulp impedes the transport of defence cells and molecules as well as systemically administered antibiotics to the infected site. The principle of complete debridement of vital and necrotic pulp tissue, removal of microorganisms and affected dentin, and cleansing of the root canal are the cornerstone in successful endodontic therapy. Recently, there have been significant technological advancements to facilitate root canal cleaning and shaping. The use for endodontic treatment causes the death of microorganism exposed by promoting damages in the envelope of the cell, and survivors require a long period of time to recover the normal growth rate that improves the beneficial effects of this treatment. One of the resistant bacteria found in persistent periapical lesions is Enterococcus fecaelis, which is capable of invading the tubular dentin even after adequate chemomechanical preparation has been performed. This has received much attention as the Enterococcus fecaelis bacteria have been found to adapt best to the

root canal system and eradication is extremely difficult. Moreover, clinical isolates of Enterococcus fecaelis recovered from root canal infections can express antimicrobial resistance to conventional treatment regimens recommended for dental procedures. Theoretical Framework Root canal therapy has been practiced ever since 1928 and the success rate has tremendously increased over the years owing to various advancements in the field. One main reason is the complete understanding of the microbiology involved in the endodontic pathology. This has helped us to modify the conventional treatment plans and effectively combat the microorganisms. Now, this study is aiming to explore the characteristics of enterococcus faecalis and to eliminate them by using apple cider vinegar. Conceptual Framework Paradigm The figure illustrates how to destroy the growth of enterococcus fecaelis on an infected vital and necrotic pulp by using apple cider vinegar.
Enterococcus Faecaelis in the pulp

Culture of enterococcus faecalis

Apple cider vinegar

Destruction of the microorganism

Statement of the Problem

Hypothesis. null Significance of the study This study aims to destroy the growth of Enterococcus fecaelis in the infected vital pulp or necrotic pulp. This study will benefit the dentist by reducing the chance of unsuccessful endodontic treatment. This study will also benefit the patient by saving their time and money. This study will prevent the Enterococcus fecaelis to metastasize to the other areas of the oral cavity. Scope and Delimitations and Limitations The researcher have selected apple cider vinegar to eliminate only the growth of microorganism E. Faecalis in the Pulp. This study focuses only on the pulp, when it is infected or necrotic because of the presence of bacteria. The researcher will

use a microscope to observe if E. Faecalis is bacteristatic or bactericidal and also they will use a culture media to place the E. Faecalis present in the pulp and test if the Apple Cider Vinegar is effective in eliminating the bacteria.