S.

Anil Kumars CV

Date: SURESH ANIL KUMAR, Ph.D [Post-Docorate] 7013, Mazarin, Montreal, Quebec, Canada H4E 2XB E-mail: anil.suresh@mcgill.ca Phone : 514 660 5799

Overview
After completing my Master of Science degree in Biotechnology from Pondichery Central University, India and Doctorate degree (Ph.D) in the subject of Biotechnology with specializations in Protein Chemistry and Bio/Nanotechnology from National Chemical Laboratory, India. I joined Prof. Jay L. Nadeaus group to pursue my further research as a postdoc, at the Department of Biomedical Engineering, McGill University, Montreal, Canada in collaboration with Prof. Yves-Alain Peter, Ecole Polytechnique. Owning to my versatile exposure, I am interested in the area of Nano-Biotechnology, Nano-Cancer, protein and carbohydrate chemistry, Microfluidics, Photonic Crystals, MEMS, structural and functional aspects of proteins, biocongugation of nanoparticles and molecular biology. I would like to be a part of a Group/Industry engaged in the synthesis of Quantum Dots (Bio as well as chemical methods), understanding the role of proteins in nanoparticles biosynthesis, protein characterization, nanoparticle production, characterization, conjugation of nanoparticles to various biologically significant molecules and application of nanoparticles in various biorelated and well as engineering applications at nano level in a multidisciplinary environment.

Career Objective
Looking for a full-time position in Research (Academic/Industrial), where I can demonstrate my technical and experimental skills and contribute to the development and better understanding of science for benefit.

Academics
University of Pune, Pune, India 2007 Ph.D. Biotechnology, Topic Biosynthesis of sulfide nanoparticles by Fusarium oxysporum, purification and characterization of enzymes and proteins responsible for the extracellular synthesis Pondichery Central University, Pondichery, India 2001 Master of Science degree in Biotechnology Osmania University, Secunderabad, AP, India 1999 Bachelor of Science degree in Botany, Zoology and Chemistry

S. Anil Kumars CV

Achievements and awards Senior Research Fellowship awarded from CSIR, India Junior Research Fellowship awarded from CSIR, India
2003 to 2006 2001 to 2003

Qualified all India entrance to pursue M.Sc. Biotechnology conducted by Jawaharlal Nehru University Area of Interest

1999 to 2001

Nanotechnology, Nano-Biotechnology, Nano-Cancer, Protein biochemistry, Physical biochemistry, Microbiology, Glycobiology, Molecular Biology, Micro Electro Mechanical Systems. Professional Experience and Work Summary Presently working as a Post-Doc from June 1st 2007, with Prof. Jay Nadeau at the Department of Biomedical Engineering, McGill University, Canada in collaboration with Prof. Yves-Alain Peter, Micro and Nano Systems Laboratory, Ecole Polytechnique on the project entitled Enhanced optical multianalyte detection lab on chip for point-of-care diagnostics the final goal of the project is to make nano-chip for the easy detection of pathogens, replacing the radioactive compounds. My specific objectives for the project are to synthesize quantum dots using a novel biological system using fungus as well as chemical methods (So far made, Biological methods: Gold, Silver, CdS, CdSe and Organometallic Synthesis: CdSe, CdTe, CdSe-ZnS core shell), characterize them using XRD, TEM, AFM, XPS, used them to conjugate to different antibodies for the detection of pathogens. Simultaneously I have been working on the other project that involve, functionalization of quantum dots to various molecules (Dopamine, Doxorubicin) and use them to specifically target the mammalian (Cancerous) and bacterial cells; that lead to phototoxicity due to generation of Reactive Oxygen Species (ROS). And also we use QDs to distinguish bacteria for example, I have conjugated the QDs to WGA (wheat germ agglutinin) for labeling of Bacteria. The WGA conjugates were used to label a mixed bacterial culture-containing Gram

S. Anil Kumars CV negative (E. coli) and Gram positive (B. subtilis) cells. It was hoped that the WGA would increase the fluorescence of the particles, since previous work in our group has found enhancement of bare-core CdSe by WGA conjugation. Worked as Research Fellow from Dec 2001 to March 2007 under the guidance of Dr. M. I. Khan, Scientist, Division of Biochemical Sciences, National Chemical Laboratory, India on the Topic entitled Biosynthesis of sulfide nanoparticles by Fusarium oxysporum, purification and characterization of enzymes and proteins responsible for the extracellular synthesis. During which I have gained experience in field of protein biochemistry and nanotechology. I have biosynthesized CdSe quantum dots extracellularly using the fungus Fusarium oxysporum, I have purified and well characterized the enzymes sulphite and nitrate reductases and capping peptide involved in the extracellular synthesis of nanoparticles. In vitro synthesis of different metal nanoparticles such as gold, silver and technologically important semiconductor nanoparticles such as CdS and CdSe nanoparticles was carried out just by using the purified enzymes and capping peptide which is a first report of its kind, the nanoparticles being characterized by UV-Vis Spectroscopy, Fluorescence spectroscopy, Time resolved fluorescence spectroscopy, X-ray diffraction (XRD), Transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS). Conjugation of the in vitro synthesized CdS quantum dots to lectins (glycoproteins) for diagnostic applications was also performed. During my post graduation I have carried out a dissertation on a project entitled Characterization of the extracellular polysaccharides produced by Xanthomonas oryzae pv. oryzae strains, the bacterial blight pathogen of rice during which I have purified the exopolysaccharides produced by different strains of bacteria Xanthomonas oryzae pv. oryzae that show varying degree of virulence among rice cultivars. I have characterized the polysaccharides by FTIR and Proton NMR and also identified the specific sugars composition by HPAEC so as to compare the exopolysaccharides composition that plays a role in the interaction with host. After my post graduation I also gained a five months of experience in Molecular Biology, carried out under the guidance of Dr. Yogesh Souche, NCCS, India during which I

S. Anil Kumars CV worked on a topic entitled Phylogeny of snakes in and around pune based on their 16 s rRNA". The objectives were to isolate DNA from different snake samples 40 (muscle), collected from different parts of Maharastra (India). Amplify the DNA by carrying out PCR (Polymerase chain reaction) using different 16 s rRNA primers (7 primers were used). Check for the PCR product, purify the PCR product, do DNA sequencing, and then phylogenetically align them using Bio-informatics programmers (BLASTA, FASTA) for easy classification and identification of Snakes. Technical Expertise Microbiology and Parasitology: Besides routine diagnostic laboratory work, Isolation, Identification, Culture and maintenance of bacteria and fungi. Molecular Biology and Biotechnology: DNA and RNA purification, Agarose gel electrophoresis, DNA cloning, PCR, DNA sequencing. Protein Chemistry and Biophysics: Standard protein purification techniques (All chromatographic techniques, HPLC, FPLC), 1-D and 2-D Electrophoresis and Blotting techniques, Rotofor Iso-electric focusing, co-immunoprecipitation as well as biophysical techniques including UV-Vis Spectroscopy, Fluorimetry, Time resolved spectrofluorimeter. Material Chemistry: X-ray diffraction (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), Xray photoelectron spectroscopy (XPS) and Atomic force microscopy (AFM) for nanoparticle characterization. Cell Culture: Cell culture (mammalian cell lines), cell toxicity assays. Instrumental Expertise XRD, TEM, SEM, FTIR Times resolved fluorescence spectrophotometer Microscopes, Confocal Microscopy Centrifuge, Ultra-Centrifuge PCR HPLC Spectrofluorimeter Electrophoresis and Blotting systems Lyophilizer DNA Sequencer FPLC Spectropolarimeter 2D Electrophoresis Fermentor Rotofor isoelectric focusing

S. Anil Kumars CV

List of Publications 1. Biosynthesis of extracellular CdSe quantum dots by the fungus Fusarium oxysporum S. Anil Kumar, Abu Ayoobul Ansary, Absar Ahmad and M. I. Khan*, Journal of Biomedical Nanotechnology, 3, 190-194, 2007. 2. Nitrate Reductase Mediated Synthesis of Silver Nanoparticles from AgNO3 S. Anil Kumar, Majid Kazemian, S. W. Gosavi, S. Kulkarni, Renu Parischa, Absar Ahmad and M. I. Khan*, Biotechnology Letters, 29, 439-445, 2007. 3. Sulphite reductase mediated synthesis of gold nanoparticles capped by phytochelatin S. Anil Kumar, Majid Kazemian, S. W. Gosavi, S. Kulkarni, Absar Ahmad and M. I. Khan* Biotechnology and Applied Biochemistry, 47, 191-195, 2007. 4. CdS Quantum dots: Enzyme mediated in vitro synthesis, characterization and conjugation to plant lectins Abu Ayoobul Ansary, S. Anil Kumar, Krishna Sastry, Majid Kazemain S. Kulkarni Absar Ahmad and M. I. Khan* Journal of Biomedical Nanotechnology, 3, 406-413, 2007. 5. Comparison of exopolysaccharides produced by Xanthomonas oryzae pv. oryzae strains, BXO1 and BXO8 that show varying degrees of virulence in rice (Oyrza sativa L.) V. B. Singh, S. Anil Kumar, S. Isaac Kirubakaran, R. Sunish Kumar and N. Sakthivel*, Journal of Phytopathology, 154, 410-413, 2006. 6. Conjugation of biosynthesized CdSe quantum dots to wheat germ agglutinin for biological applications S. Anil Kumar, Yves-Alain Peter and Jay Nadeau* Communicated to Langmuir. 7. One-Step extracellular biosynthesis of heterogeneously shaped gold nanocrystallites and their separation S. Anil Kumar, Yves-Alain Peter and Jay Nadeau* Under Review, ACS Nano. 8. The relationship of QD composition and conjugate to cellular uptake and toxicity Jay L. Nadeau*, Anil Kumar Suresh, Rafael A. Khatchadourian, Eve-Marie Dumas and Samuel J. Clarke SPIE, Jan 2008. 9. CdSe-Zns core shell nanoparticles: Enzymatic synthesis and Characterization S. Anil Kumar, Yves-Alain Peter and Jay Nadeau * (Manuscript under preparation).

S. Anil Kumars CV Symposium and Conference Participated 1. XVII International Symposium on Glycoconjugates, IISc, Bangalore, India Jan 12- 16, 2003. 2. XXXIII National Seminar on Crystallography, NCL, Pune, India, Jan 8-10, 2004. 3. Prospecting of Fungal Diversity and Emerging Technologies. Agharkar Research Institute, Pune, India, Feb 6-7, 2003. 4. International Conference on Particles-Based Device Technologies, Particles 2007. Hotel Delta Chelsea, Toronto, Canada, 18-21 August 2007. Presentations Oral 1. SPIE, San Jose, California, USA Jan 20-24, 2008. The relationship of QD composition and conjugate to cellular uptake and toxicity Jay L. Nadeau, Anil Kumar Suresh, Rafael A. Khatchadourian, Eve-Marie Dumas and Samuel J. Clarke. 2. Nanotechnology in Medical Physics and Cancer Research, A mini-symposium sponsored by the Cancer Axis of the MUHC, Montreal, Canada July 5th 2007, Introduction to Nanotechnology: Biosynthesis of nanoparticles, purification and characterization of enzymes involved S. Anil Kumar and Jay Nadeau. 3. 32nd annual meeting of the Mycological Society of India, JGI, India Feb 2-3, 2006, In vitro synthesis of nanoparticles using the purified enzymes and proteins from Fusarium oxysoporum S. Anil Kumar, Ahmad. A and Khan. M.I. 4. Indo-French meting held at Sophia-Antipolis, France Sep 8-10, 2004, Extracellular synthesis of nanoparticles by the fungus Fusarium oxysporum M. I. Khan, Absar Ahmad, Murali Sastry, Rajiv Kumar, S. Anil Kumar and S. Senapati. 5. Indo-French meting held at Goa, India Feb 4-7, 2006 In vitro synthesis of nanoparticles using enzymes M. I. Khan, S. Anil Kumar, P. Nithya Priya, Shadab Ali Khan and Absar Ahmad. 6. National Seminar on Nanobiotechnology held at NBRI, Lucknow, India Nov 17-19, 2005, In vitro synthesis of inorganic nanoparticles using enzymes and proteins from microbes Absar Ahmad, M. I. Khan and S. Anil Kumar.

S. Anil Kumars CV 7. National seminar on Nanoscience and Nanobiotechnology, Pondichery Central University, Pondichery, India Dec 5-6, 2005, In vitro synthesis of nanomaterials using enzymes and proteins from fungi and actinomycetes Absar Ahmad, M. I. Khan and S. Anil Kumar. 8. Half day symposium on Bio-mimetic Materials, MRSI, Pune, India Nov 10th, 2005, In vitro synthesis of nanoparticles using enzymes M. I. Khan, S. Anil Kumar and Absar Ahmad.

Poster 1. XVII International Symposium on Glycoconjugates, Indian Institute of Science, Bangalore, India Jan 12-16, 2003. Chickpea lectin, a lectin with complex sugar specificity Sameer, Atul, S. Anil Kumar, Feroz Khan, Absar Ahmad and M. I. Khan 2. Eighth International conference on nanostructured materials (Nano 2006). Indian Institute of Science, Bangalore, India, 2025 August, 2006. In vitro synthesis of different metal nanoparticles using the purified enzymes and proteins from Fusarium oxysporum. S. Anil Kumar, Abu Ayoobul Ansary, Absar Ahmed and M. I. Khan

Personal particulars Name Date of Birth Permanent Address : Suresh Anil Kumar : July 25th, 1979 : 7013 Mazarin, Montreal, Quebec, Canada H4E 2X8 Temporary Address : C/O Prof. Jay Nadeau Room No. 717 Department of Biomedical Engineering 3775 Lyman Duff Medical Building McGill University, Montreal Quebec, Canada H3A 2B4

S. Anil Kumars CV Phone Mobile E-mail Personal Languages known : 514 398 4921 (Office) : 514 660 5799 : anil.suresh@mcgill.ca : Married, Male, Indian : English (read, write, speak) Telugu (read, write, speak) Hindi (read, write, speak)

References Jay Nadeau Associate Professor Department of Biomedical Engineering 3775 Lyman Duff Medical Building McGill University, Montreal Quebec, H3A 2B4 CANADA Phone: 514 398 8372 E-mail: jay.nadeau@mcgill.ca Yves-Alain Peter Associate Professor Micro and Nano Systems Laboratory Engineering Physics Department Ecole Polytechnique de Montral P.O. Box 6079, Station Centre-Ville Montral, Qubec H3C 3A7 CANADA Phone : + 1 514 340 4711 ext. 3100 E-mail: yves-alain.peter@polymtl.ca

S. Anil Kumars CV M. Islam Khan Scientist Biochemical Sciences Division, National Chemical Laboratory, Pune 411 008 Maharshtra, INDIA. Phone: +91 20 25902241 E-mail: mi.khan@ncl.res.in Absar Ahmed Scientist Biochemical Sciences Division, National Chemical Laboratory, Pune 411 008, Maharshtra, INDIA. Phone: +91 20 25902226 E-mail: a.ahmad@ncl.res.in