Scribd Logo
Carica

Benvenuto in Scribd!

  • Print Microbiology Lab Quiz

    Caricato da

    John Chahoud
    99 visualizzazioni2 pagine
    1. The document provides instructions and information related to gram staining of bacteria. It includes questions about the components of bacterial cell walls, the steps of the gram staining procedure, and what the results of gram staining can indicate. 2. Gram staining involves using crystal violet, iodine, decolorizing agent (ethanol), and safranin stains to distinguish between gram-positive and gram-negative bacteria based on their cell wall structure. Gram-positive bacteria appear purple and gram-negative bacteria appear pink. 3. The gram stain was invented in 1884 by Danish scientist Hans Christian Gram and is still used today to identify unknown bacteria samples.

    Descrizione originale:

    Titolo originale

    Print › Microbiology Lab Quiz

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Formati disponibili

    PDF, TXT o leggi online da Scribd

    Hai trovato utile questo documento?

    Questo contenuto è inappropriato?

    Segnala questo documento
    1. The document provides instructions and information related to gram staining of bacteria. It includes questions about the components of bacterial cell walls, the steps of the gram staining procedure, and what the results of gram staining can indicate. 2. Gram staining involves using crystal violet, iodine, decolorizing agent (ethanol), and safranin stains to distinguish between gram-positive and gram-negative bacteria based on their cell wall structure. Gram-positive bacteria appear purple and gram-negative bacteria appear pink. 3. The gram stain was invented in 1884 by Danish scientist Hans Christian Gram and is still used today to identify unknown bacteria samples.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Scarica in formato PDF, TXT o leggi online su Scribd
    Segnala contenuti inappropriati
    99 visualizzazioni2 pagine

    Print Microbiology Lab Quiz

    Caricato da

    John Chahoud
    1. The document provides instructions and information related to gram staining of bacteria. It includes questions about the components of bacterial cell walls, the steps of the gram staining procedure, and what the results of gram staining can indicate. 2. Gram staining involves using crystal violet, iodine, decolorizing agent (ethanol), and safranin stains to distinguish between gram-positive and gram-negative bacteria based on their cell wall structure. Gram-positive bacteria appear purple and gram-negative bacteria appear pink. 3. The gram stain was invented in 1884 by Danish scientist Hans Christian Gram and is still used today to identify unknown bacteria samples.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Scarica in formato PDF, TXT o leggi online su Scribd
    Segnala contenuti inappropriati
    di 2

    1.

    Bucteriu reuct diIIerent


    bused on whut on their cells?
    SurIuce composILIon
    2. Gm- cell wulls huve u higher
    _____ content thun Gm+ cell
    wulls?
    IpId
    3. Grum-negutive cell wulls
    contuin two...? Which ure?
    Whut's in the middle?
    Membrunes; Inner und
    ouLer; pepLIdogIycun
    4. Grum-positive cell wulls
    contuin only one...?
    Membrune
    5. How do you solve Ior Iinul
    Concentrution?
    CUJo.1 m
    6. How do you solve Ior O.T.
    Concentrution?
    |CUJo.1 m|J|1J1o,ooo|
    7. How muny test tubes do you
    use Ior dilution?
    ;
    8. How much oI the broth
    sumple do we obtuin und
    where do we put it?
    We obLuIn 1.o m; puL IL In
    LIe 1o^-1 LesL Lube
    9. How much sterile wuter do
    you put in the , test tubes?
    q.o m
    10. It is best to use cultures Ior
    Grum stuining within whut
    time period?
    zq Iours
    11. Nume the Iour stuining
    reugents.
    CrysLuI VIoIeL, odIne,
    ELIunoI, SuIrunIn
    12. The bucteriul cell wull is
    composed oI whut? Where is
    it locuted?
    PepLIdogIycun; on Lop oI LIe
    pIusmu membrune
    13. The exterior oI the cells huve
    whut kind oI churge; unionic
    or cutionic?
    AnIonIc
    14. Whut ure the steps to using
    the spreuding rod?
    DIp In uIcoIoI, puss
    LIrougI burner; IeL cooI;
    spreud specImen
    15. Whut ure the two groups oI
    bucteriu und their colors?
    Grum-posILIve und Grum-
    neguLIve bucLerIu; purpIe
    und pInk, respecLIveIy
    16. Whut ure the two stuining
    techniques?
    SImpIe und dIIIerenLIuI
    17. Whut did we use us the
    counter-stuin?
    SuIrunIn
    18. Whut did we use us the
    decolorizing ugent?
    ELIunoI
    19. Whut did we use us the
    mordunt?
    odIne
    20. Whut did we use us the
    primury stuin?
    CrysLuI VIoIeL
    21. Whut do we do euch time we
    trunsIer the broth Irom test
    tube to test tube?
    Use u new pIpeLLe
    22. Whut do you do uIter diluting
    the test tubes? How much do
    we obtuin? 1o^-q turns into?
    DIIuLe uguIn InLo peLrI
    dIsI; o.1 m; 1o^-
    23. Whut do you lubel the test
    tubes Ior dilution?
    1o^-1 --> 1o^-;
    24. Whut does CIL stund Ior und
    whut does it meun?
    CoIony ormIng UnILs;
    LIe number oI coIonIes
    grown durIng IncubuLIon
    25. Whut does Gm-[Gm+ do help
    us determine?
    ApproprIuLe unLIbIoLIc
    LreuLmenLs
    26. Whut does it meun to uliquot
    something?
    Tuke u smuIIer voIume oI
    u sumpIe
    27. Whut does TITC stund Ior und
    whut ure the numericul
    vulues?
    Too Iew Lo counL; <o
    28. Whut does the Crystul Violet
    stuin do within the cell wull?
    orms u purpIe compIex
    29. Whut does the ecolorizing
    Solution, Ithunol, do to Grum-
    negutive bucteriu? Why?
    WusIes ouL LIe purpIe
    compIex. CeII wuIIs ure
    Loo LIIn
    30. Whut does the ecolorizing
    Solution, Ithunol, do to Grum-
    positive bucteriu?
    NoLIIng, becuuse oI LIe
    LIIck ceII wuIIs
    31. Whut does TNTC stund Ior und
    whut ure the numericul
    vulues?
    Too numerous Lo counL;
    >oo
    32. Whut huppens to the cell wull
    iI they ure old or ure stressed
    by heut or cold?
    TIe ceII wuIIs become
    LIIn
    33. Whut is u diluent? Give un
    exumple.
    IuId used Lo dIIuLe
    concenLruLIon sumpIe;
    wuLer
    34. Whut is u dilution Iuctor? rucLIon by wIIcI
    orIgInuI Is dIIuLed
    35. Whut is the purpose oI
    SuIrunin to Grum-negutive
    bucteriu?
    Turns IL pInkJred
    36. Whut is the purpose oI the
    mordunt, Iodine?
    Keeps LIe CrysLuI VIoIeL
    Irom beIng removed Irom
    LIe Grum-posILIve
    bucLerIu
    37. Whut is the purpose oI the
    mordunt?
    odIne compIexes LIe
    crysLuI vIoIeL Lo LIe
    pepLIdogIycun Iuyer
    38. Whut kind oI dye is Crystul
    Violet? Cutionic or Anionic?
    CuLIonIc
    M|crob|o|ogy Lob Qu|z; Grom Sto|n
    Study online at qu1ze1.com/_1pd5
    39. Whut yeur wus the grum stuin invented? 188z
    40. Who invented the grum stuin? Huns CIrIsLIun Grum
    41. Why is grum stuining importunt? denLIIIes unknown bucLerIu

    Potrebbero piacerti anche