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THE JOURNAL

OF

BIOCHEMISTRY
EDITED FOR THE JAPANESE BIOCHEMICAL SOCIETY VOLUME 75, 1974

EDITORIAL BOARD
Editor-in-Chief: Haruhisa YOSHIKAWA Editors
Setsuro EBASHI Yasutomi NISHIZUKA Kazutomo IMAHORI Shosaku NUMA Shin-ichi ISHII Ryo SATO Yoshito KAZIRO Ikuo YAMASHINA

Managing Editors
' Goro KIKUCHI Yoshiaki MIURA

Regular Reviewers
Yasuhiro ANRAKU Takahiko HOSHITA Akira ISHIHAMA Yasuo KAGAWA Yoshimasa KYOGOKU Takashi MURACHI Yoshitsugu NOSE Takamitsu SEKINE Yasuyuki TAKAGI Gakuzo TAMURA Koichi YAGI Toshio YAMANO Kozo HAMAGUCHI Akira ICHIHARA Koichi IWAI Masayasu KITAGAWA Shigeki MINAKAMI
Kei NAGANO

Tsuneo OMURA Kazuo SHIBATA Kenji TAKAHASHI Shigeru TSUIKI Yaauo YAGI Zensaku YOSHIZAWA

Tadao HORIUCHI Tokuji IKENAKA Sadaaki IWANAGA Jiro KOYAMA Yasuji MINODA Shoshichi NOJIMA Toshiaki OSAWA Masamiti TATIBANA. Shosuke TAKEMURA. Daisuke TSURU Tamio YAMAKAWA Yasumi YUGARI

PUBLISHED BY

MONTHLY

THE JAPANESE BI0CMEM1CAL SOCIETI

COPYRIGHT, 1974

BY

THE JOURNAL OF BIOCHEMISTRY

PUBLISHED BY

THE JAPANESE'BIOCHEMICAL SOCIETY

CONTENTS OF VOLUME 75

No. 1, January, 1974


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Membrane-associated Adenosine 3', 5'-Monophosphate-dependent Protein Kinase and Its Substrates in Human Erythrocytes. R. Shimomura, S. Matsumura, and Y. Nishizuka 1 Purification and Properties of S-100 Protein from Porcine Brain. T. Abe, K. Takahashi, and T. Ando 11 Identification of Rapidly Trinitrophenylating Amino Groups of Human Bence-Jones Proteins. T. Shinoda and Y. Tsuzukida 23 Studies on Low Temperature Spectra of Respiratory Pigments. II. Spectra of Cytochromes in Respiratory Systems between Liquid Helium and Room Temperatures. B. Hagihara, R. Oshino, and T. Iizuka 45 Hormonal Regulation of Tyrosine Transaminase Synthesis in Isolated, Rat Liver Perfused with Synthetic Medium. . H. Ohtsuka 53 a+ Sensitivity of Esckerichia coli to Viral Nucleic Acid. VII. Further Studies on Ca -induced Competence. A. Taketo and S. Kuno 59Formation and Metabolism of Acetoacetate in Yeast. /. Shimizu, J. Nagai, and H. Katsuki 69% Biosynthesis of Seminolipid: Sulfation in Vivo and in Vitro. S. Handa, K. Yamato, I. Ishizuka, A. Suzuki, and T. Yamakawa 7T Enzymatic Hydrolysis of Glucans Containing /J-1,3- and /3-1,6-linkages. III. Gibberella -1,6Glucan 6-Glucanohydrolase Operative in the Selective Hydrolysis of ^-1,3-Glucosidic Linkages in Eisenia Laminaran. Y. Shibala 85 Studies on the Regulation of Glutathione Level in Rat Liver. iV. Tateishi, T. Higashi, S. Shinya, A. Naruse, and Y. Sakamoto 93 A Bacterial Dextranase Releasing Only Isomaltose from Dextrans. T. Sawai, K. Toriyama, and K. Yano 105 Collagen Fibrillogenesis in Vitro: An Accelerative Effect of Surfactants on Collagen Fibril Formation. M. Honya and H. Mizunuma 113: Studies on the Binding of Thiamine Pyrophosphate to Apoenzyme of Yeast Pyruvate Decarboxylase. H. Sanemori, S. Yoshida, and T. Kawasaki 123' Mechanism of Adrenaline-induced Lipolysis in Adipose Tissue. H. Okiida, Y. Saito, N. Matsuoka, and S. Fujii 13T L-Histidine Ammonia-lyase in Rat Liver. I. Purification and General Characteristics. H. Okamura, T. Nishida, and H. Nakagawa 139Comparison of the Primary Structure of Whale Pancreatic Ribonuclease with Ribonuclease A. T. Yamada, Y. Nakazawa, and late T. Ukita 153 Agglutination of Bacterial Spheroplast. III. Relationship of Labelled Concanavalin A Binding to the Agglutinability. H.B. Maruyama 165 Interaction of Human Serum Proteinase Inhibitors with Proteolytic Enzymes of Animal, Plant, and Bacterial Origin. M. Sasaki, H. Yamamoto, H. Yamamoto, and S. Iida 171 Physicochemical Properties of Deoxyribonucleic Acid from an Extreme Thermophile. T. Oshima and K. Imahori 179 i

CONTENTS OF VOLUME 75
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SHORT COMMUNICATIONS Characterization of Arainopeptidase and One Dipeptidase of Cytoplasmic Soluble Fraction from Mycobacterium phlei. M.T. Plancot and K.-K. Han 185 Changes in Adenosine 3', 5'-Monophosphate Level and Protein Kinase Activity by Glucagon in Rat Liver Nuclei. H. Higashino and M. Takeda 189 Inhibition of the Biosynthesis of Leucomycin, a Macrolide Antibiotic, by Cerulenin. S. Omura and H. Takeshima 193 Further Investigations on the Chemical Structure of Forssman Globoside Obtained from Caprine Erythrocyte Stroma. T. Taketomi, A. Hara, N. Kawamura, and M. Hayashi 197 Effects of Temperature, Salts, and Solvents on the Enzymatic Activity of Ribonuclease T i . K. TakahasM 201 Isolation and Characterization of Vicia faba Root Tip Deoxyribonucleic Acid. J.B. Engle and O.G. Ward 205 PRELIMINARY NOTE -A. Spin-label Study on Calcium-induced Conformational Changes of Troponin Components. S. Ebashi, S. Ohnishi, S. Abt, and K. Maruyama 211

No. 2, February, 1974 Mechanism of Inhibition of Bacterial Growth by Adenine. R. Hosono and S. Kuno 215 Iron Binding Proteins Found in Duodenal Mucosa of Rats during Iron Transport. Y. Yoshino and Y. Hiramatsu 221 Transport of Sugars and Amino Acids in Bacteria. VIII. Properties and Regulation of the Active Transport Reaction of Proline in Escherichia coli. A. Morikawa, H. Suzuki, and Y. Anraku 229 Transport of Sugars and Amino Acids in Bacteria. IX. Studies on the Active Transport Reaction in Sodium Azide- and 2,4-Dinitrophenol-sensitive Mutants of Escherichia coli. F. Goto and Y. Anraku 243 -Activation of Serum Enzymes by the Antigen-Antibody Reaction and Its Relation to Cl Inhibitor. H. Sumi and S. Fujii 253 Inactivation of Rhizopus chinensis Acid Protease by Some Diazo Compounds. D. Tsuru, K. Fujiwara, R. Watanabe, T. Yoshimoto, S. Hayashida, M. Tomimatsu, and Y. Okoshi 261 Separation of Transfer RNA's from Bacillus subtilis, Bacillus stearothermophilus, and Chicken Liver by Polyacrylamide Gel Electrophoresis. F. Varricchio and T. Seno 269 Physicochemical Studies on the Light Chain3 of Myosin. II. Effect of Metal Ions on the Electrophoretic Behavior of Myosin Subunits. ' S.L. Gaffin and A. Oplatka 277 Reaction Mechanism of the Ca'+-dependent ATPase of Sarcoplasmic Reticulum from Skeletal Muscle. X. Direct Evidence for Ca1+ T r a n s l a t i o n Coupled with Formation of a Phosphorylated Intermediate. M. Sumida and Y. Tonomura 283 T h e Stability of Taka-araylase A Immobilized on Various Sizes of Matrix. T. Horigomt, H. Kasai, and T. Okuyama 299 Tree-boundary Electrophoresis of Sodium Dodecyl Sulfate-Protein Polypeptide Complexes with Special Reference to SDS-Polyacrylamide Gel ElectrophoresiB. K. Shirahama, K. Tsujii, and T. Takagi 309 Studies on 3,4-Dihydroxyphenylacetate-2,3-dioxygenase. II. Role of Sulfhydryl Groups and Subunit Structure. M. Ono-Kamimoto and S. Senoh 321

CONTENTS OF VOLUME 75 '-Studies on D-Tetrose Metabolism. IV. Purification and Some Properties of D-Erythrulose Reductase from Beef Liver. K. Uehara, T. Tanimoto, and H. Sato Human Kidney a-Galactosidase. Multiplicity and Enzyme Activities for Ceramide Trihexoside and Some Aryl o-Galactosides. /. Kano and T. Yamakawa Regulation of Rabbit Liver Fructose-l,6-diphosphatase. II. Modification by Lysosomal Cathepsin B, from the Same Cell. K. Nakashima and K. Ogino M-Protein. T. Masaki and O. Takaiti 'Effect of Phenol Derivatives and Chemical Modification on the Adenosine Triphosphatase Activities of Heavy Meromyosin and Subfragment 1. T. Kameyama, S. Hayakawa, and T. Stkine Studies on the Active Site of Brevibacterium Pyrocatechase. K. Nagami and S. Stnoh 'Studies on the Molecular Complex of Flavins. V. Possible Role of Free Sulfhydryl Group in Apoprotein of Glucose Oxidase and 6-Amino Group in Adenine Moiety of FAD. H. Tsuge and H. Mitsuda Partial Purification and Some Properties of Cyclamate Sulfamatase. T. Niimura, T. Tokieda, and T. Yamaha -Carboxamidomethylatidn of Ribonuclease from Rhiapus sp. T. Komiyama and M. Irie

iii
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333 347 355 367 381 389

399 407 419

"SHORT COMMUNICATIONS Evidence for the Role of Disulfide Bonds in the Masking of Chromophore Groups in Bovine t-Casein. K. Shimazaki and S. Arima 429 Application of the Optical Rotation Stopped-flow Method to Kinetic Studies of Rapid Conformational Changes of Proteins. pH-jump of Taka-amylase A. K. Hiromi, M. Ohnishi, and Y. Hama 433 T h e Presence of a 0-Mannosidic Linkage in a Glycopeptide from Microsomal Membranes of Rat Liver. T. Kawasaki, K. Sugahara, T. Okumura, and I. Yamashina 437

No. 3, March, 1974 Circular Dichroism of the Disulfide Groups of Ribonuclease in 6 M Guanidine Hydrochloride. T. Takagi and T. Izutsu The Amino Acid Sequence of SH-peptides Involved in the Active Site of Myosin A Adenosinetriphosphatase. T. Yamashita, Y. Soma, S. Kobayaski, and T. Sekine Inactivation of Bovine Carboxypeptidase A by Specific Modification of Arginine Residues with Phenylglyoxal. H. Ikenaga and K. Takahashi Viscometric Demonstration of Tubulin Polymerization. R. Kuriyama and H. Sakai Purification and Some Properties of f-Glutamyl Transpeptidase from Azo Dye-induced Hepatoma. N. Taniguchi Biochemical Studies on Gramicidin S Non-producing Mutants of Bacillus brevis ATCC 9999. M. Kambe, Y. Irnat, and K. Kurahashi Multiple Components of /5-N-Acetylhexosarainidase from Equine Kidney. Their Action on Glycolipids and Allied Oligosaccharides. Y. Seyama and T. Yamakawa The Broad Aglycone Specificity of a-L-Fucosidases from Marine Gastropods. M. Nishigaki, T. Muramatsu, A. Kobata, and K. Maeyama biochemical Studies on Sulfate-reducing Bacteria. XIII. Sulfite Reductase from Dtsulfovibrio vulgarisMechanism of Trithionate, Thiosulfate, and Sulfide Formation and Enzymatic Properties. K. Kobayashi, Y. Seki, and M. Ishimoto

441 447 455 463 473 481 495 509

519

CONTENTS OF VOLUME 75
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Biogenesis of Multiple Cellulase Components of Pstudomonai fluorescent var. ctllulosa. I. Effects of Culture Conditions on the Multiplicity of Cellulase. T. Yoshikawa, H. Suzuki, and K. Nisizawa 53T Purification of a New Plasma Inhibitor of Kallikrein. H. Sumi and S. Fujii 541 On the Conformation of NADP-dependent Isocitrate Dehydrogenase from Bacillus stearothermophilus. Y. Hibino, Y. Nosoh, and T. Samejima 55$ Purification and Kinetic Studies of Wheat Bran /3-Amylase. Evaluation of Subsite Affinities. M. Kato, K. Hiromi, and Y. Morita 56$ Adaptive Control of the Ethanol-forming System in Heterolactic Acid Bacteria. Effect of Growth Conditions on Alcohol Dehydrogenase Synthesis in Leuconostoc mtsenteroides. S. Ho, H. Hashiba, and Y. Eguchi 577 Studies on Luciferase from Photobacterium phosphoreum. V. An Enzyme-FMN Intermediate Complex in the Bioluminescent Reaction. K. Yoshida, M. Takahashi, and T. Nakamura 583 Mechanism of Mercuric Chloride Resistance in Microorganisms. III. Purification and Properties of a Mercuric Ion Reducing Enzyme from Escherichia coli Bearing R Factor. K. Izaki, Y. Tashiro, and T. Funaba 59/ Characteristics of the Inhibition of Ornithine-5-aminotransferase by Branched-chain Amino Acids. T. Malsuzawa 601 A Method for the Detection of Strand Breaks in DNA Samples. K. Shimada, T. Nishimoto, and Y. Takagi 611 Studies on Acyl Donors for Plasma Cholesterol Esterification. Triglyceride Fatty Acids in Rats in Vivo. M. Sugano 619 Studies on Violet-colored Acid Phosphatase of Sweet Potato. I. Purification and Some Physical Properties. K. Uehara, S. Fujimoto, arid T. Taniguchi 627 Studies on Violet-colored Acid Phosphatase of Sweet Potato. II. Enzymatic Properties and Amino Acid Composition. K. Uehara, S. Fujimoto, T. Taniguchi, and K. Nakai 639 SHORT COMMUNICATIONS Time-dependent Increase in Stability of Collagen Fibrils Formed in Vitro. Effect of Temperature. T. Hayashi and Y. Nagai Reaction of Chloroplasts with Diazonium-1,2,4-triazole. K. Nakaya, K. Ariga, F. Obata, and Y. Nakamura N-Acyl Specificity of Taka-N-acetyl-^-D-glucosaminidase Studied with Synthetic Substrate Analogs. III. Effects of a-Hydroxyl and <r-Methoxyl Substituents of the Substrate-2-acetamide Group on Enzymatic Hydrolysis. K. Yamamoto Biphasic Effect of Taurocholate on Lipase Activity in the Presence of Lecithin. K. Tsuzurahara, T. Hiramatsu, and S. Takeyama States of Imino Groups in Collagen as Measured with a Modifying Reagent, /erf-Butyl Hypochlorite. S. Yamazaki, A. Matsushima, A. Tsuchii, and Y. Inada

65T 655

659 663 667

PRELIMINARY NOTES Staining of Myofibrils by Fluorescent Antibody against the 10 S Component of the Original or-Actinin Preparation. H. Sugita, T. Masaki, and S. Ebashi 671 Isolation of an Inhibitor of /3-Glucuronidase from Human Saliva. W. Sakamoto, O. Nishikaze, and E. Sakakibara 675

CONTENTS OF VOLUME 75

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No. 4, April, 1974 Effect of Isoproterenol on Polyamine Metabolism in Mouse Salivary Glands. H. Inoue, H. Tanioka, K. Shiba, A. Asada, Y. Kato, and Y. Takeda An Affinity Column Method for Partial Purification of Cytochrome P-450 from Phenobarbitalinduced Rabbit Liver Microsomes. Y. Itnai and R. Sato Interaction of the o-Ketoglutarate Dehydrogenase Complex with Basic Polypeptides. K. Imai and Y. Tomita /3-Galactosidases from Jack Bean Meal and Almond Emulsin. Application for the Enzymatic Distinction of Gall-4GlcNAc and Gal^l-^GlcNAc Linkages. M. Arakawa, S. Ogata, T. Muramatsu, and A. Kobata Comparison of Adaptations to Diet of Enzymes Involved in Uric Acid Production from IMP in Chickens and Rats. R. Itoh and K. Tsushima Purification of Rabbit Liver Cathepsin Bi. K. Ogino and K. Nakashima Preparation of Plasminogen Activator from Vascular Trees of Human Cadavers. Its Comparison with Urokinase. . N. Aoki Nuclear Phosphoprotein Kinases from Rat Liver. M. Takeda, S. Matsumura, and Y. Nakaya Localization of Troponin in Thin Filament and Tropomyosin Paracrystal. /. Ohtsuki The Effects of Chemical Modification by N-Bromosuccinimide of Saccharifying a-Amylase from Bacillus subtilis on Various Substrates. H. Fujimori, M. Ohnishi, and K. Hiromi Sulfated Glycoproteins Capable of Coagulating Calcium Carbonate Isolated from Pathological Human Bile. H. Nagashima, M. Masubuchi, and Z. Yosizawa Evidence Obtained by Cathepsin Digestion of Microsomes for the Assembly of Cytochrome bt and Its Reductase in the Membrane. A. Ho Properties of Heart Sarcolemmal Na+-K+ ATPase. D.B. McNamara, P.V. Sulakhe, J.N. Singh, and N.S. Dhalla Studies on Soybean Trypsin Inhibitors. IX. Reconstitution of an Active Derivative from Two Inactive Fragments and the Contribution of Tryptophan-93. T. Koide, T. Ikenaka, K. Ikeda, and K. Hamaguchi The Trypsin-catalyzed Hydrolysis of Some L-a-Amino-lacking Substrates. R. Kobayashi and S. Ishii Chemical Structure of Glycolipid of Guinea Pig Red Blood Cell Membrane. . Y. Seyama and T. Yamakawa Preparation of an Active Fragment of Potato Proteinase Inhibitor Ha and Its Properties. T. Iwasaki, T. Kiyohara, and M. Yoshikawa Studies on the Conversion of Mevalonate into Bile Acids and Bile Alcohols in Toad and the Stereospecific Hydroxylation at Carbon Atom 26 during Bile Alcohol Biogenesis. T. Kuramoto, S. Itakura, and T. Hoshita L-Ascorbic Acid Sulfate Esters with Special Reference to Enzymatic Hydrolysis. H. Hatanaka, Y. Ogawa, and F. Egami Induction of Tyrosine Aminotransaminase and Ornithine Decarboxylase in Isolated Perfused Regenerating Rat Liver. W. Fukui, A. Fujita, H. Ohtsuka, and Y. Miura Reactivity of Peptide Imino Groups in Proteins with /<r/-Butyl Hypochlorite in Relation to Secondary Structure. A. Matsushima, S. Yamazaki, and Y. Inada Stabilization of Pig Kidney Cathepsin A by Sucrose and Chloride Ion, and Inhibition of the Enzyme Activity by Diisopropyl Fluorophosphate and Sulfhydryl Reagents. E. Dot Cathepsin A of Two Different Molecular Sizes in Pig Kidney. E. Dei, Y. Kawamura, T. Matoba, and T. Hata

679 689 699

707 715 723 731 743 753 767 779 787 795

805 825 837 843

853 861 867 875 881 889

CONTENTS OF VOLUME 75
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Sensitivity of Escherichia coli to Viral Nucleic Acid. VIII. Idiosyncrasy of CaI+-dependent Competence for DNA. A. Taketo 895 Phosphodiesterase-phosphomonoesterases from Fusarium moniliforme. IV. Kinetic Studies. H. Yoshida 905

Molecular Properties of Phosphoenolpyruvate Carboxylase of Escherichia coli W. T. Yoshinaga, H. Teraoka, K. Izui, and H. Katsuki 913 SHORT COMMUNICATIONS Effect of Quasi-substrate on the Monomer-dimer Equilibrium of D-Amino Acid Oxidase. K. Horiike, K. Shiga, A. Isomoto, and T. Yamano 925 N-Acyl Specificity of Taka-N-acetyl-^-r>glucosaminidase. IV. Essential Requirement of the 2-Acetamide-carbonyl Group for the Enzymatic Hydrolysis. K. Yamamoto 931 Circular Dichroism Studies on the Interaction of the Lac Repressor with Poly d(A-T) and Nucleic Acid Components. K. Fukada, Y. Ohshima, and T. Horiuchi 935 Properties of Human Erythrocyte Acetylcholinesterase. PRELIMINARY NOTES Partial Purification of a Carbon Monoxide-binding Hemoprotein Reconstitutively Active in Fatty Acid co-Hydroxylation from Porcine Kidney Cortex Microsomes. K. Ichihara, E. Kusunose, and M. Kusunose 943 Relationships among the Multiple Components of /!-N-Acetylhexosaminidase from Equine Kidney. Y. Seyama and T. Yamakawa 947 K. Sihotang 939

No. 5,

May, 1974

Properties of Bacterial Virus <f>A and Its DNA. Pyridine-2,6-dicarboxylic Acid (Dipicolinic Acid) Formation in Bacillus sublilis, Formation of Dipicolinic Acid from Pyruvate and Aspartic Semialdehyde.

A. Takelo 951 I. Non-enzymatic K. Kimura 961

Subsite Affinities of Bacterial Liquefying a-Amylase Evaluated from the Rate Parameters of Linear Substrates. S. Iwasa, H. Aoshima, K. Hiromi, and H. Hatano 969 Action of Trypsin on Synthetic Substrates. Trypsin. IV. Hydrolyses of Synthetic Insulin Fragments by A'. Nishino, N. Mitsuyasu, and N. Izumiya 979

Regulation of 3-Deoxy-D-araiino-heptulosonate-7-phosphate Synthetase in Brevibacterium flavum. I. Shiio, S. Sugimoto, and R. Miyajima 987 Studies on Nitrite Reductase from Spinach Leaves. I. Further Purification and Some Properties. J. Shimizu and G. Tamura 999 Induction of 3-Aminolevulinate Synthetase in Organ Culture of Chick Embryo Liver by Allylisopropylacetamide and 3, 5-Dicarbethoxy-l,4-dihydrocollidine. Y. Tomita, A. Ohashi, and G. Kikuchi 1007 Isolation of Nuclei from a Tetraploid Strain of Saccharomyces cerevisiae. Adenylate Kinase from Pseudomonas denitrificans. Collagen Fibrillogenesis in Vitro. Formation. K. Doi and A. Doi JO 17 I. Purification and Antiserum Inhibition. H. Terai 1027

The Functional Role of Non-ionic Materials in Collagen Fibril M. Honya 1037

Studies on Polypeptide Elongation Factor 2 from Pig Liver. I. Purification and Properties. K. Mizumoto, K. Iwasaki, M. Tanaka, and Y. Kaaro 1047

CONTENTS OF VOLUME 75

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Studies on Polypeptide Elongation Factor 2 from Pig Liver. II. Molecular Weight and Amino Acid Composition. K. Mizumoto, K. Iwasaki, Y. Kaaro, C. Nojiri, and Y. Yamada 1057 Carboxypeptidase in Commercial Bromelain Powder. Partial Purification and Some Properties of Pineapple Carboxypeptidase. E. Doi, C. Ohtsuru, and T. Matoba 1063 Studies on Sterol-ester Hydrolase in Rat Liver Homogenates. T. Sakamoto, H. Okuda, and S. Fujii 1073 The Polypeptide Compositions of Bovine Cytochrome Oxidase and Its " Proteinase - treated" Derivative. T. Yamamoto and Y. Orii 1081 Isolation of Na+,K+-ATPase-associated Plasma Membrane of Pig Thyroid Cells. Y. Nagai and T. Hosoya 1091 Sedimentation Behavior of F-Actin of Various Types. M. Kikuchi, K. Maruyama, and H. Noda 1103 Mechanism of the Reversible Glycine Cleavage Reaction in Arthrobacter giobiformis. I. Purification and Function of Protein Components Required for the Reaction. H. Kochi and G. Kikuchi 1113 l+ Mechanism of Adenosine Triphosphate-dependent Ca Uptake of Brain Microsomes. K. Nakamura and K. Konishi 1129 The Nucleases from Acrocylindrium sp. IV. Purification and Properties of Exonuclease. /. Suhara 1135 Polymerization of Salmonella Flagellin in Water and Deuterium Oxide Media. Y. Uratani 1143 A Spin-label Study of the Photosynthetic Bacterium, Rhodospirillum rubrum. Reduction and Regeneration of Nitroxide Spin-labels. K. Maruyama and S. Ohnishi 1153 SHORT COMMUNICATIONS Effects of Stearic Spin-labels on the Photochemical Activities of Chromatophores from Rhodospirillum rubrum. K. Maruyama and S. Ohnishi 1165 Nucleotide Sequence of the Anticodon Region of Torulopsis Phenylalanine Transfer RNA. S. Takemura, H. Kasai, and M. Colo 1169 A Modified Lithium Buffer System for Analysis of Acidic and Neutral Amino Acids in Physiological Fluids. T. Sato and T. Iijima 1173 An Improved Mutarotase Assay Using /)-D-Glucose Oxidase and an Oxygen Electrode. /. Miwa and J. Okuda 1177 Temperature Jump Relaxation Studies of Oxygen Binding to Isolated Chains of Hemoglobin. T. Nakamura, Y. Sugita, and Y. Yoneyama 1181 PRELIMINARY NOTES Morphology of Myosin Molecules from Developing Chick Embryo. T. Obinata and K. Takahashi 1183 Partial Purification and Reconstitution of the Heme Oxygenase System from Pig Spleen Microsomes. T. Yoshida, S. Takahashi, and C. Kikuchi 1187 Synthesis of Cardiac Myosin by a Polyribosome Fraction from Chick Embryonic Skeletal Muscle. T. Masaki and T. Kinoshita 1193 Determination of pKa's of Individual Histidine Residues in Pancreatic Ribonuclease by HydrogenTritium Exchange. M. Ohe, H. Matsuo, F. Sakiyama, and K. Narita 1197

No. 6,

June, 1974

Studies on the Microsomal Electron-transport System of Anaerobically Grown Yeast. I. Intracellular Localization and Characterization. Y. Yoshida, H. Kumaoka, and R. Sato 1201

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CONTENTS OF VOLUME 75
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Studies on the Microsomal Electron-transport System of Anaerobically Grown Yeast. II. Purification and Characterization of Cytochrome b,. Y. Yoshida, H. Kumaoka, and R. Sato 1211 Evidence for the Identity of O-Acetylserine Sulfhydrylase with O-Acetylhomoserine Sulfhydrylase in Yeast. S. Yamagaia, K. Takeshima, and N. Naiki 1221 The Chain Compositions of Several Invertebrate Collagens. S. Kimura and F. Matsuura 1231 Purification of Arylsulfatase A from Boar Testis and Its Activities toward Seminolipid and Sulfatide. K. Yamato, S. Handa, and T. Yamakawa 1241 Studies on Luciferase from Photobacterium phosphoreum. VI. Stoichiometry and Mode of Binding of FMNH, and O, to Stripped Luciferase. T. Watanabe, G. Tomita, and T. Nakamura 1249 Purification and Some Properties of Formate Dehydrogenase. T. Ohyama and I. Yamazaki 1257 Cytochrome c-552 and Cytochrome c-554 Derived from Nitrosomonas europaea. Purification, Properties, and Their Function in Hydroxylamine Oxidation. T. Yamanaka and M. Shinra 1265 Thiols of Myosin. II. Chemical Modification of Sulfhydryl Groups of Myosin A Adenosine Triphosphatase with Diazobenzenesulfonic Acid. /. Kabasawa, K. Murayama, M. Kimura, and T. Sekirte 1275 Enzymic Degradation Products of <u-Heparin (Whale Heparin). N. Ototani, K. Nakamura, and Z. Yosizawa 1283 Pyrophosphatidic Acid. A New Phospholipid from Cryptococcus neoformans. T. Itoh and H. Kaneko 1291 Radioimmunoassay for Phenobarbital. H. Satoh, Y. Kuroiwa, A. Hamada, and T. Uematsu 1301 Fatty Chains of Acyl, Alkenyl, and Alkyl Phosphoglycerides of Rabbit Sarcoplasmic Reticulum. The Metabolic Relationship Considered on the Basis of Structural Analyses. K. Waku, H. Ho, T. Bito, and Y. Nakazawa 1307 Cation-binding Sites of Brain Microsomes. D. Satomi 1313 Effect of Glucose and Adenosine 3', 5'-Cyclic Monophosphate on the Early Development of Dictyostelium discoideum. K.O. Yanagisawa, Y. Tanaka, and K. Yanagisawa 1321 Studies on Lipolysis in Lipid Micelles Isolated from Adipose Tissue. Y. Saito, H. Okuda, and S. Fujii 1327 Bromocresol Purple as a Probe for Structural Features of Fragmented Sarcoplasmic Reticulum. Y. Nakamaru and Y. Sugii 1331 Nucleoside Catabolism in Streptococcus hemolylicus. A. Taheto and Y. Taketo 1341 Phosphorylation of Deoxyribonucleosides and DNA Synthesis in Early Cleaving Embryos of the Sea Urchin. N. Suzuki and Y. Mano 1349 Studies on ESR Spectra of Chromatophores from Rhodospirillum rubrum. T. Higuti, T. Shiga, T. Kakuno, and T. Horio 1363 SHORT COMMUNICATIONS Synthesis of Catalase in Liver Slices from Aminotriazole-pretreated Rats. A. Nakamura and S. Minakami 1373 Polymerization of Papain by the Reaction of Its Tyrosine Residues with Tetranitromethane. S. Tsukamoto and M. Ohno 1377 The Inactivation of Ribonuclease from Seminal Vesicles by Photooxidation and Carbethoxylation.
M. IrU 1381

Effect of Ethyl Chlorophenoxyisobutyrate (CPIB) on Plasma Lipoprotein Lipase Activity. H. Okuda and S. Fujii 138S Digestibility of the Active Fragment of Potato Proteinase Inhibitor Ha by Bovine Chymotrypsin and a Bacterial Proteinase. T. Iwasaki, I. Iguchi, T. Kiyohara, and M. Yoshikawa 1381 Inhibition of Chicken Erythrocyte AMP Deaminase by Blue Dextran 2000. M. Yoshino, Y. Kawamura, and N. Ogasawara 1391

CONTENTS OF VOLUME 75

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PRELIMINARY NOTES Occurrence of Separate Pools of Phosphatidic Acid for Biosynthesis of Phospholipids in Escherichia colt Membranes. M. Ishinaga, M. Kato, and M. Kilo 1395 Decrease in Molecular Weight of Cytoplasmic DNA Polymerase on Treatment with Broraelain plus RNase and DNase. T. Shiosaka, M. Aoki, and S. Fujii 1399 Sphingosine Bases of Gangliosides in Juvenile Amaurotic Family Idiocy (Spielmeyer-Vogt Type). T. Taketomi and N. Kawamura 1403 Studies on the Nucleotide Sequence of Pseudouridine-containing 5S RNA from Saccharomyces cerevisiae. M. Mtyazaki 1407

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