Material Safety Data Sheet (MSDS) for Proteins

166B Cummings Center s Beverly, MA 01915 Orders s 877-616-CELL (2355) orders@cellsignal.com Support s 877-678-TECH (8324) info@cellsignal.com Web s www.cellsignal.com

I.

Identification
Phospho-specific antibodies2000, 3000 and 9000 series Protein markers7000 series Protein kinases, inhibitors and substrates6000 series

Vapor pressure (mm Hg): N/A Solubility in water: Liquids readily miscible in water

Product/Common name: See list below (used on label)

IV.

Fire and Explosion Hazard Data

Auto-ignition temperature: N/A Flash point: N/A Flammable limits in Air (% by volume): N/A Percent volatile by volume (%): N/A Extinguishing media: Fire extinguishing media should match other materials in fire. Special fire fighting procedures: If involved in fire, don NIOSH/MSHAapproved self-contained breathing apparatus, flame/chemical resistant. Unusual fire and explosion hazards: None known

Chemical name: Protein in buffer Formula: Complex polypeptides Chemical family: Protein

II.

Ingredients

Material: Hazardous components in water solutions. Buffer components are listed on product data sheet. Principal Hazardous Component CAS Chemical & Common Name Number (2-[N-morpholinoethanesulfonic acid) (MES). . . . . . . . . . #145224-94-8. . . . . . . . . . 0.50 mM 2-mercaptoethanol . . . . . . . . . . . . . . . #60-24-2 . . . . . . . . . . . . . 020 mM 7-amino-4-methylcoumarin . . . . . . . . #26093-31-2 . . . . . . . . . . . 01 mM ATP . . . . . . . . . . . . . . . . . . . . . . . . . . #51963-61-2 . . . . . . . . . 00.1 M Brij-35 . . . . . . . . . . . . . . . . . . . . . . . . #9002-92-0 . . . . . . . . . . . . . 0.10% Bromophenol blue . . . . . . . . . . . . . . . #62625-28-9 . . . . . . . . . . . . 0.1% Caffeine . . . . . . . . . . . . . . . . . . . . . . . #58-08-2 . . . . . . . . . . . . . 050 mM Dithiothreitol (DTT) . . . . . . . . . . . . . . #27565-41-9 . . . . . . . . . . 050 mM Dodecyl sodium sulfate . . . . . . . . . . . #151-21-3 . . . . . . . . . . . . . . . 06% EDTA . . . . . . . . . . . . . . . . . . . . . . . . . #60-00-4 . . . . . . . . . . . . . 010 mM EGTA . . . . . . . . . . . . . . . . . . . . . . . . . #67-42-5. . . . . . . . . . . . . . 0.10 mM Ethylene glycol. . . . . . . . . . . . . . . . . . #107-21-1 . . . . . . . . . . . . . . 0.30% Glycerol . . . . . . . . . . . . . . . . . . . . . . . #56-81-5 . . . . . . . . . . . . . . . 050% Imidazole hydrochloride. . . . . . . . . . . #1467-16-9 . . . . . . . . . . 0250 mM Nonidet P40 . . . . . . . . . . . . . . . . . . . . #9002-93-1 . . . . . . . . . . . . 00.1% Sodium azide . . . . . . . . . . . . . . . . . . . #26628-22-8 . . . . . . . . . . . . . 1 mM Tris-HCl . . . . . . . . . . . . . . . . . . . . . . . #1185-53-1 . . . . . . . . . . . 050 mM Triton X-100 . . . . . . . . . . . . . . . . . . . . #9036-19-5 . . . . . . . . . . . 00.15% Tween-20 . . . . . . . . . . . . . . . . . . . . . . #9005-64-5 . . . . . . . . . . . . . 0.10% Complex polypeptides . . . . . . . . . . . . . . . . . . . . . . . . . . . Biological activity varies (%) quantity measured by activity

V.

Health Hazard Data

Threshold Limit Value (TLV) and source: Data not available Chemical listed as carcinogen or potential carcinogen: No OSHA permissible exposure limit: None ACGIH Threshold Limit Value: None I.A.R.C. Monographs: No National Toxicology Program 2-mercaptoethanol (CAS #60-24-2)Oral LD50 rat 330 mg/kg; skin absorption LD50 rat 0.015 ml/kg Bromophenol blue (CAS #62625-28-9)Suspected carcinogen; Handle with care! Caffeine (CAS #58-08-2)LD50 rat 240 mg/kg EDTA (CAS #60-00-4)Intraperitoneal LD50 rat 379 mg/kg; intraperitoneal LD50 mouse 250 mg/kg Glycerol (CAS #56-81-5)Oral LD50 rabbit 27 g/kg Sodium azide (CAS #26628-22-8)Oral man 29 mg/kg (LDLO); oral woman 14 mg/kg (LDLO) Target Organ Data Glycerol (CAS# 56-81-5)Behavioral; headache; gastrointestinal; nausea or vomiting Acute effects of overexposure: May cause skin irritation. To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated. Allergy-prone individuals should be particularly cautious with proteins or other materials of biologic origin. Solutions containing sodium azide (CAS #26628-22-8) may be fatal if initially swallowed or absorbed through skin. Swallowing: May be harmful if swallowed. Skin absorption: May be harmful if absorbed through the skin. page 1 of 2

III.

Physical Data

Boiling point: Decomposes Vapor density (Air = 1): N/A

2004 Cell Signaling Technology, Inc.

Appearance and odor: Colorless and odorless Melting point: N/A Freezing Point: N/A Vapor Pressure at 20C: N/A Specific gravity (H2O = 1): Varies Evaporation rate: N/A

Material Safety Data Sheet (MSDS) for Proteins

166B Cummings Center s Beverly, MA 01915 Orders s 877-616-CELL (2355) orders@cellsignal.com Support s 877-678-TECH (8324) info@cellsignal.com Web s www.cellsignal.com

Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Skin contact: May be harmful. Eye contact: May cause eye irritation. Chronic effects of Overexposure: May be harmful. Allergic reactions in certain sensitive individuals may develop. Emergency and First Aid Procedures SwallowingWash out mouth with water, provided person is conscious. Call a physician. SkinIn case of contact, immediately wash skin with soap and copious amounts of water. InhalationIf inhaled, remove to fresh air. If breathing becomes difficult, call a physician. EyesIn case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating eyelids with fingers. Consult a physician.

IX.

Special Precautions

Precautions to be taken in handling and storage: This compound is sold only for research use by personnel familiar with the toxicology of organic chemicals and who are well trained in good laboratory habits, such as avoiding spills, keeping hands clean at all times and not rubbing eyes with hands while working in the laboratory. This compound is sold only in milligram quantities for use in dilute solutions in biological research. No other use is intended, and any other use may involve substantial hazards. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide for experienced personnel. Cell Signaling Technology, Inc., shall not be held liable for any damage resulting from the handling of or from contact with the above product. The burden of safe use of this material rests entirely with the user.

Revised: August 2001

VI.

Reactivity Data

Stability: Any degradation products are not hazardous. Stability/Conditions to avoid: Not reactive as far as is known Incompatibility/Materials to avoid: None known Hazardous Decomposition Products: None known Hazardous polymerization: Will not occur

VII. Spill or Leak Procedures

Steps to be taken if material is spilled or released: Wear lab coat, chemical resistant gloves and safety glasses. Wipe up spill with a suitable absorbant and dispose of properly. Wash down the spill site. Waste disposal method: Dispose of in accordance with all federal, state and local regulations.

VIII. Special Protection Information

Respiratory protection: Not required. Use mechanical exhaust. Ventilation: Use mechanical exhaust. Protective equipment: Good laboratory practices should always be used. Wear suitable protective clothing, chemical resistant gloves and lab safety glasses.
2004 Cell Signaling Technology, Inc.

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