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INTRODUCTION
phenomenon of symbiosis. The biotic and abiotic elements of nature are all
interdependent. The plants are indispensable to man for his life. The three important
necessities of life – food, clothing and shelter – and a host of other useful products are
supplied to him by the plant kingdom. Nature has provided a complete a store house of
remedies to cure all ailments of mankind. The knowledge of drugs has accumulated over
effective means of ensuring health care 1. Some ancients as Assyrians, Arabs, Egyptians
and Greeks left a legacy over a thousand years of medicinal plants use. They have
discovered not only their medicinal properties, but also some dose-related effects of
new drugs from natural products is still considered important. This seems to be even
more relevant for the developing countries, where the costs to develop a drug are
prohibitive. Since 1980, the World Health Organization has been encouraging countries
Scientific strategies for the study of natural products from plants have
changed substantially in the past few years for a number of reasons, including advance in
technology, new molecules of interest, changing ethical principles for organism collection
and heightened awareness of the chemical and biological potential of the natural
resources.
The last couple of years have seen a resurgence interest in the use of herbal
products. The World Health Organization (WHO) estimates that about 80% of the
medicines; the medicinal plants play a major role and constitute the backbone of the
traditional medicine. Indian materia medica includes 2000 natural products of therapeutic
importance of which 400 are of mineral and animal origin and rest are of vegetable
origin.
called the “Botanical Garden of the World”. There are very few medicinal herbs of
commercial importance, which are not cultivated or collected in this country. There are
approximately 1250 Indian medicinal plants, which are used in formulating therapeutic
screening programs of the natural resources have been initiated by various government
and private agencies. Among these was the screening program by the Central Drugs
Research Institute (CDRI) initiated in 1964 with a spanning period of 25 years and
Medicinal herbs have been in use for thousands of years, in one or another
form under the indigenous system of medicine. The main Indian traditional system of
medicine namely Ayurveda, Siddha and Unani are primarily plant based system. Since
products.
materials has again opened a vast area for research and development. Modern research in
the herbal field is now focused on terms of standardization of plant material, extracts and
specially prepared mixtures where the biological activity is used as a measure of their
quality. Such a change has occurred for several different reasons, including the
availability for biological activity, although some time it is difficult to measure because
of interfering substance.
Today plant based medicines are only 9.5% of the estimated 250,000
species worldwide. Therefore, the field is still wide open. Plants are recognized as rich
source of medicines because they produce molecules that have intrinsic biological
Compared with the synthetic drugs, however, the criteria and the approach
for herbal drugs are much more complex. Several aspects which do not exist with
synthetic drugs influence the quality. The problems specific to the phytopharmaceuticals
natural origins are ever on the increase. We should be aware of the fact that some global
pharmaceutical companies have already engaged themselves in the intense and systematic
sense of urgency in response to the expanding human population and its subsequent
demands for food, good health and increasing areas of land on which to live. Nature
recognizes no artificial barriers such as those of the “academic disciplines” and thus it is
no surprise to find investigators with quite different academic training studying various
facilitates the solution of specific problems such as those in plant toxicology but may also
enhance the diversity and consequent value of bioactive natural product research.
The use of herbal and other naturally based medicines has a long history.
However the utilization of whole plant or other crude preparations for the therapeutic or
• Variation in the amount of the active constituent with geographic areas, from one
season to another, with different plant parts and morphology, and with climatic
Thus the isolation of natural products that have biologic activity towards
Extraction from the specific plant material is a trial and error exercise in which different
solvents are tried under a variety of conditions such as time and temperature of
extraction. The success or failure of the extraction process is monitored by the most
appropriate assay.
Once extracted from the plant, the bioactive component then has to be
separated from the coextractives. This may involve simple crystallization of the
compound from the crude extract or more usually it may involve further solvent partition
properties of the desired compound, such as acidity, polarity and molecular size.
Structure Determination
numerous sources, each of which gives some structural information, and the assimilation
of these data into a chemical structure that uniquely fits all the available structural
information. A wide range of spectroscopic instrumentation, such as UV, IR, and visible
medicinal and aromatic plant, but which may not be the bioactive principles in the plant 6.
physiological constituent of crude drug, which can be easily isolated and quantified
• It should be characteristic of (or unique to) the given crude drug or the herbal
preparation.
method.
markers for quality assurance if they meet the criteria for analytical markers (eg.
crude drugs, but it does not guarantee the bioactivity or efficacy of the crude drugs.
material monitored by a biological assay, till the compound responsible for the biological
activity (in the bioassay used) is/are isolated in the pure form.
compounds can be labeled as “BIOMARKER” for the crude drug. But if the bioassay
Department of Pharmacognosy – PESCP 8
INTRODUCTION
used is specific for a particular bioactivity, then the isolated compound can be called as
developing countries for primary healthcare because of their wide biological activities,
higher safety margins and lesser costs. In India, the herbal drug market is about $ one
billion and the export of plant based crude drugs is around $ 80 million. A need has
therefore been generated for enforcing more stringent quality control on such products
and for this, the plant material grown, harvested and processed for manufacturing should
be as per the standardized protocols to ensure product uniformity, efficacy and safety.
quality oriented herbal products, care should be taken right from the cultivation stage
conditions.
methods, may not correlate with the biological or pharmacological activities claimed,
methods, the herbal products may not produce the claimed pharmacological activity. The
Chromatographic fingerprinting has been in use for a long time for single
industry has been used to highlight impurity profiles, thereby giving indications of the
identification of herbal material or extract. Techniques such as HPLC, TLC, GC, CE have
been used for identity tests. Spectroscopic methods such as IR, NMR, UV-Vis may also
conventional pharmaceutical manufacturing criteria for assay and content uniformity may
be applied. These marker compounds may be used to help identify herbal materials, set
specifications for raw materials, standardize botanical preparations during all aspects of
manufacturing processes, and obtain stability profiles. Again the HPLC analysis for
fingerprints 13.
Unani and Siddha system of medicines since a long time. The various literature surveys
related to Hemidesmus indicus (L.) R.Br. shows that the major compounds (as seen on
TLC/HPLC) which can be used for the quality control, are not highlighted and there is
lacking of analytical methods also. The literature surveys also indicate that there is
possibility of isolating new compounds from roots and other parts of the plant. Although
Lupeol and Vanillin can be used as markers for this plant, but these phytoconstituents are
not unique for this plant and they are found in large number of other plants also. Hence it
was decided to carry out further research work in the field of isolation and
(Asclepiadaceae). Here in the present study attempts had been made to isolate and
standardize the constituents which are unique to this plant and characterize them as
markers.