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INSTRUCTION SHEET DYS058

Faecal Parasite Concentrator - Solvent Free

Midi Parasep SF

Without ether or ethyl acetate


For the clean and efficient concentration of: Helminth ova and larvae Protozoal cysts and oocysts Single use device for in vitro diagnostic use only

Health and Safety Benefits


Totally enclosed/sealed process Solvent free protocol Disposable device Single use, no sample contamination Pre-filled systems available

Performance Benefits
Optimum sample recovery Enhanced sample clarity Rapid four step process Human resources optimised Reduces human error

Midi Parasep SF Solvent Free

Faecal Parasite Concentrator

Midi Parasep SF Solvent Free Faecal Parasite Concentrator

Mixing Chamber (High Clarity Polypropylene)

Self standing sample chamber Filtration matrix

Integral Spoon

Patented Filter Thimble (High Density Polyethylene)


A two stage filtration matrix. Large particles are rejected without occluding the 425m pores. Recovery rate with Parasep is comparable to traditional sieve method, ie: Ridley-Allen.

Debris Trap

Rejected particles are trapped to prevent extrusion into the Sedimentation Cone during centrifugation.

Air/Liquid Seal and Safety Lock

The seal prevents the release of biohazardous material. The lock ensures the Mixing Chamber and Filter Thimble are removed together for safe disposal.

Fat Dispersion Chamber

A perforated fat dispersion chamber (EC Des.App.000512017) removes the smaller faecal debris and separates the fat content so that it can be efficiently removed from the resulting sediment without the use of ether or ethyl acetate.
Patient ID

Sedimentation Cone (High Clarity Polypropylene) Centrifuge Compatibility

Sediment forms in the base of the cone allowing examination for the presence of helminth eggs or larvae and protozoal cysts or oocysts. Designed to fit all 50ml centrifuge buckets.

INSTRUCTION SHEET DYS058

Midi Parasep SF Solvent Free

Faecal Parasite Concentrator


Procedure
See label for storage conditions and expiry date

Please adhere to the following guidelines when handling Parasep. To avoid cross contamination the Parasep device should remain closed at all times except when introducing the sample or when retrieving the final concentrated sample for examination.

1. Sample Preparation
Fresh Sample
If required unscrew lid and add 8ml of 10% formalin to the mixing chamber and one drop of surfactant (eg: Triton X-100) to emulsify. Introduce a pea sized (0.5g) faecal sample (equal to one level spoons if using the spoon on the end of the Parasep). Mix in thoroughly with the Parasep spoon.

3. Centrifugation
Invert Midi Parasep and centrifuge at 200g for two minutes. Midi Parasep fits all 50ml centrifuge buckets.

Debris

Preserved Sample

Shake or vortex the incoming sample to mix. Transfer 0.5ml equal to 0.5g of sample into Midi Parasep SF mixing chamber. Add 7.5ml of 10% formalin/water plus 2 drops of Triton X. Screw on filter thimble and sedimentation cone, etc.

Fat dispersion chamber 10% Formalin

Sediment

2. Emulsification
Seal Midi Parasep by screwing in the filter thimble and conical tube. Vortex or shake to emulsify with the sedimentation cone pointing upwards. The faecal sample may be left for 24hr to soften and ensure complete bacteriocidal action has taken place. Note: To calculate the required RPM for any centrifuge.

4. Examination
Direct Method Unscrew and discard the filter thimble and mixing tube. Pour off all the liquid above the sediment. Pipette one drop of saline or Iodine solution onto a slide, add one drop of deposit to the saline or Iodine. Mix sample and cover with cover-slip. (The whole of the deposit must be examined). OR FE Workstation Method Unscrew and discard the filter thimble and mixing tube. Pour off all the liquid above the sediment. BioHazard Add 1ml of saline to the sediment. Shake or vortex to re-suspend sample. Insert Aspirator into suspension and press SAMPLE to Re-suspended sample draw 80l into the Optical Slide. (Refer to FE instruction manual).

RPM - rotor speed in revs/min. g - centrifugal force (max.1000g) r - radius, horizontal distance between sedimentation cone tip and spindle centre measured in mm.

DISCARD IN ACCORDANCE WITH YOUR STANDARD AND LOCAL OPERATING PROCEDURE FOR CLINICAL WASTE

SINGLE USE DEVICE

HARMFUL

INSTRUCTION SHEET DYS058

Midi Parasep SF Solvent Free Faecal Parasite Concentrator

Midi Parasep SF Solvent Free

Faecal Parasite Concentrator

Midi Parasep SF Solvent Free Faecal Parasite Concentrator

Ordering Information
Product
Midi Parasep SF (requires fixative only)
Midi Midi Midi Midi Parasep Parasep Parasep Parasep SF (devices supplied without reagent) SF (each mixing tube contains 8ml 10% Formalin and one drop of Triton X) SF pre-filled with SAF & Triton x SF pre-filled with Parasafe

Storage Temp
4-35C 15-35C 15-35C 15-35C 4-35C 15-35C 15-35C 15-35C 4-20C 15-35C 4-35C 15-35C 15-35C 4-20C

Pack Size
50 40 40 40 50 40 40 40 40 40 40 40 40 40

Code
149900 149910 149920 149930 145000 145100 145200 145300 145400 145500 145600 145700 145800 145900

Midi Parasep (requires fixative and ethyl acetate)


Midi Parasep (Without liquid filling, including 50 devices and 50 storage lids) Midi Parasep (including 6.0ml of Parasafe ecological fixative) Midi Parasep (each mixing tube contains 6ml 10% Formalin and one drop of Triton X) Midi Parasep (each mixing tube contains 6ml 10% Formalin and one drop of Triton X) Midi Parasep (each mixing tube contains 6ml of 10% Formalin and 1 drop of Triton X) and 80ml bottle of ethyl acetate fat solvent) Midi Parasep (including 6.0ml of Sodium Acetate-Acetic Acid Formalin Solution) Midi Parasep (including mesh) Midi Parasep (including mesh and each mixing tube contains 6ml 10% Formalin and one drop of Triton X) ) Midi Parasep (each mixing tube contains 6ml 10% Formalin and one drop of Triton X) Midi Parasep (each mixing tube contains 6ml 10% Formalin and one drop of Triton X) and 80ml bottle of ethyl acetate fat solvent)

Product
Fixatives
Formalin 10% Neutral Saline Buffered Sodium Acetate-Acetic Acid Formalin Solution (SAF) Fixative Schaudinn's Fixative 10% Formalin Fixative in water Merthiolate-lodine-Formalin (MIF) Polyvinyl Alcohol (PVA)

Code
1460 1461 1462 1463 1465 1466

Product
Stains
Eosin/Saline Acridine Orange (Acetate Buffered) Auramine Phenol (Lempert) Field Stain-Solution A Field Stain-Solution B Giemsa Stain-Rapid Lugol's lodine-Aqueous Iron Haematoxylin-Solution A Iron Haematoxylin-Solution B Trichrome For Microsporidia Trichrome For Protozoa Carbol Fuchsin for modified Z/N Stain (cold Kinyoun) Malachite Green Methylene Blue Modified Z/N Stain Pack (cold Kinyoun) Gram lodine Gram Stain Pack Crystal Violet Safranin Grams Fuchsin Lugols Iodine Grams Decolouriser Grams Carbol Fuchsin Carbol Fuchsin for standard Z/N stain

Code
1467 1480 1481 1482 1483 1484 1486 1487 1488 1489 1490 1500 1501 1502 1503 1491 1504 1492 1493 1495 1486 1476 1505 1506

Reagents
Mayers Glycerin/Albumin Physiological Saline Triton X-100 Solution Ethyl Acetate Acetone Neutral Red DPX Mounting Media Immersion Oil 1470 1471 1472 1473 1475 1494 1520 1521

General Microbiology Stain Reagents

Order product direct from DiaSys or appointed distributor


DiaSys updates its home pages on the Internet regularly with new product announcements, applications and company news. Visit our web page for all the latest information: http://www.diasys.com or e-mail on: sales@diasys.com

Unit 5, The Sapphire Centre, Fishponds Road, Wokingham, Berkshire, RG41 2QL, England Tel: +44 (0)118 9795 566 Fax: +44 (0)118 9795 186

V3 08/2010

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