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Midi Parasep SF
Performance Benefits
Optimum sample recovery Enhanced sample clarity Rapid four step process Human resources optimised Reduces human error
Integral Spoon
Debris Trap
Rejected particles are trapped to prevent extrusion into the Sedimentation Cone during centrifugation.
The seal prevents the release of biohazardous material. The lock ensures the Mixing Chamber and Filter Thimble are removed together for safe disposal.
A perforated fat dispersion chamber (EC Des.App.000512017) removes the smaller faecal debris and separates the fat content so that it can be efficiently removed from the resulting sediment without the use of ether or ethyl acetate.
Patient ID
Sediment forms in the base of the cone allowing examination for the presence of helminth eggs or larvae and protozoal cysts or oocysts. Designed to fit all 50ml centrifuge buckets.
Please adhere to the following guidelines when handling Parasep. To avoid cross contamination the Parasep device should remain closed at all times except when introducing the sample or when retrieving the final concentrated sample for examination.
1. Sample Preparation
Fresh Sample
If required unscrew lid and add 8ml of 10% formalin to the mixing chamber and one drop of surfactant (eg: Triton X-100) to emulsify. Introduce a pea sized (0.5g) faecal sample (equal to one level spoons if using the spoon on the end of the Parasep). Mix in thoroughly with the Parasep spoon.
3. Centrifugation
Invert Midi Parasep and centrifuge at 200g for two minutes. Midi Parasep fits all 50ml centrifuge buckets.
Debris
Preserved Sample
Shake or vortex the incoming sample to mix. Transfer 0.5ml equal to 0.5g of sample into Midi Parasep SF mixing chamber. Add 7.5ml of 10% formalin/water plus 2 drops of Triton X. Screw on filter thimble and sedimentation cone, etc.
Sediment
2. Emulsification
Seal Midi Parasep by screwing in the filter thimble and conical tube. Vortex or shake to emulsify with the sedimentation cone pointing upwards. The faecal sample may be left for 24hr to soften and ensure complete bacteriocidal action has taken place. Note: To calculate the required RPM for any centrifuge.
4. Examination
Direct Method Unscrew and discard the filter thimble and mixing tube. Pour off all the liquid above the sediment. Pipette one drop of saline or Iodine solution onto a slide, add one drop of deposit to the saline or Iodine. Mix sample and cover with cover-slip. (The whole of the deposit must be examined). OR FE Workstation Method Unscrew and discard the filter thimble and mixing tube. Pour off all the liquid above the sediment. BioHazard Add 1ml of saline to the sediment. Shake or vortex to re-suspend sample. Insert Aspirator into suspension and press SAMPLE to Re-suspended sample draw 80l into the Optical Slide. (Refer to FE instruction manual).
RPM - rotor speed in revs/min. g - centrifugal force (max.1000g) r - radius, horizontal distance between sedimentation cone tip and spindle centre measured in mm.
DISCARD IN ACCORDANCE WITH YOUR STANDARD AND LOCAL OPERATING PROCEDURE FOR CLINICAL WASTE
HARMFUL
Ordering Information
Product
Midi Parasep SF (requires fixative only)
Midi Midi Midi Midi Parasep Parasep Parasep Parasep SF (devices supplied without reagent) SF (each mixing tube contains 8ml 10% Formalin and one drop of Triton X) SF pre-filled with SAF & Triton x SF pre-filled with Parasafe
Storage Temp
4-35C 15-35C 15-35C 15-35C 4-35C 15-35C 15-35C 15-35C 4-20C 15-35C 4-35C 15-35C 15-35C 4-20C
Pack Size
50 40 40 40 50 40 40 40 40 40 40 40 40 40
Code
149900 149910 149920 149930 145000 145100 145200 145300 145400 145500 145600 145700 145800 145900
Product
Fixatives
Formalin 10% Neutral Saline Buffered Sodium Acetate-Acetic Acid Formalin Solution (SAF) Fixative Schaudinn's Fixative 10% Formalin Fixative in water Merthiolate-lodine-Formalin (MIF) Polyvinyl Alcohol (PVA)
Code
1460 1461 1462 1463 1465 1466
Product
Stains
Eosin/Saline Acridine Orange (Acetate Buffered) Auramine Phenol (Lempert) Field Stain-Solution A Field Stain-Solution B Giemsa Stain-Rapid Lugol's lodine-Aqueous Iron Haematoxylin-Solution A Iron Haematoxylin-Solution B Trichrome For Microsporidia Trichrome For Protozoa Carbol Fuchsin for modified Z/N Stain (cold Kinyoun) Malachite Green Methylene Blue Modified Z/N Stain Pack (cold Kinyoun) Gram lodine Gram Stain Pack Crystal Violet Safranin Grams Fuchsin Lugols Iodine Grams Decolouriser Grams Carbol Fuchsin Carbol Fuchsin for standard Z/N stain
Code
1467 1480 1481 1482 1483 1484 1486 1487 1488 1489 1490 1500 1501 1502 1503 1491 1504 1492 1493 1495 1486 1476 1505 1506
Reagents
Mayers Glycerin/Albumin Physiological Saline Triton X-100 Solution Ethyl Acetate Acetone Neutral Red DPX Mounting Media Immersion Oil 1470 1471 1472 1473 1475 1494 1520 1521
Unit 5, The Sapphire Centre, Fishponds Road, Wokingham, Berkshire, RG41 2QL, England Tel: +44 (0)118 9795 566 Fax: +44 (0)118 9795 186
V3 08/2010