Lab Report- Results

Tube Number 0 1 2 3 4 5 6

Concentration of CoCl2 (mg/mL) 0 1 10 20 30 40 50

CoCl2 Stock (mL) 0 0.1 1.0 2.0 3.0 4.0 5.0

Distilled H2O (mL) 10.0 9.9 9.0 8.0 7.0 6.0 5.0

Table 8.1 This table shows volumes of Cobalt Chloride stock solution (100mg/mL) and water used to prepare seven known dilutions used in our experiment. Wavelength 350 nm 420 nm 460 nm 490 nm Table 8.2 Shows our results for the absorbance of CoCl2 (50mg/mL) Notice that the absorbance fluctuates to its peak and then lowers again as the wavelength increases. Absorbance 0.028 0.133 0.625 0.881 Wavelength 530 nm 570 nm 610 nm 660 nm Absorbance 0.896 0.193 0.071 0.046

The chart above includes our data from table 8.2 to show the absorption spectrum of cobalt chloride (CoCl2). Here you are able to see the fluctuating data in detail.

Concentration of standards Absorbance (mg CoCl2/mL) 1 0.003 10 0.118 20 0.307 30 0.337 40 0.546 50 0.897

Peak wavelength= 530 530 530 530 530 530 530

Table 8.3 The data shown includes absorbance values for the six solutions of known concentration of CoCl2 at the peak absorbance wavelength seen in table 8.2

Tube Number Unknown Table 8.4 6

Absorbance 0.508

Concentration (mg/mL) 40

In table 8.4 we see the measurements of absorbance and concentration for an unknown solution of CoCl2
Wavelength 350 nm 420 nm 460 nm 490 nm Absorbance 0.802 0.841 0.280 0.090 Wavelength 530 nm 570 nm 610 nm 660 nm Absorbance 0.245 0.322 0.301 0.069

Table 8.5 The table above contains absorbance values for a plant extract containing chlorophyll. Observe that as the wavelengths change the absorbance has highs and lows with a wavelength of 420 nm as the peak.

Figure 8.6, shows absorption spectrum of chlorophyll; notice that the lower the wavelength is, the higher the absorbance of chlorophyll will be.