Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
List Price $162 UML Bookstore $231? Internet as low as $85 ? Fifth edition 1998 Sixth ed. just out 2007 Excellent reference book
Website
http://faculty.uml.edu/David_Ryan/84.314/ Syllabus=coursedescription Schedule Materials=LectureSlides, Handouts,Videosfrom lastyear
Nobel Prizes
William H. Bragg Francis W. Aston Friderik Pregl analysis of crystal structure by means of X-rays(physics) for his discovery, by means of his mass spectrograph, of isotopes (Chemistry) invention of the method of micro-analysis of organic substances (Chemistry) his research on electrophoresis and adsorption analysis, especially for his discoveries concerning the complex nature of the serum proteins (Chemistry) development of new methods for nuclear magnetic precision measurements and discoveries (Physics) Invention of partition chromatography (Chemistry) discovery and development of the polarographic methods of analysis (Chemistry) development of radioimmunoassays of peptide hormones (Physiology or Medicine) contribution to the development of high-resolution electron spectroscopy (physics) design of the scanning tunneling microscope (physics) 1915 1922 1923
Arne Tiselius Felix Bloch Edward M. Purcell Archer J P Martin Richard L M Synge Jaroslav Heyrovsky Rosalyn Yalow Kai M. Siegbahn Gerd Binnig Heinrich Rohrer
1948
Colors Boiling or Melting Points Precipitation Classical Extraction Distillation Solubilities Odors Optical Gravimetric Analytical Methods Volumetric Physical Properties Conductivity Instrumental Electrode Potential Light Absorption or Emission Mass-to Charge ratio Fluorescence Chromatographic Electrophoretic
Precision
The precision ofa measurement system,also called reproducibilit y or repeatability,is thedegreetowhich repeated measurements underunchanged conditionsshowthe same results.
accuracy of a measurement system is the degree of closeness of measurements of aquantity to its actual (true) value.
Bias Biasprovidesameasureofthesystematic,ordeterminate,error ofan analyticalmethod. = Where isthepopulationmeanfortheconcentrationofananalyte isthetruevalue Sensitivity Sensitivityofamethodorinstrumentisameasureofitsabilityto discriminatebetweensmalldifferences. Twofactorslimitsensitivity
Theslopeofcalibrationcurve Reproducibilityorprecisionofthemeasuringdevice S=mc+Sbl
Dynamicrange
Skipthefollowing chapters
Chapter2 ElectricalComponentsandCircuits Chapter3 OperationalAmplifiersinChemical Instrumentation Chapter4 DigitalElectronicsandMicrocomputers
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SignaltoNoiseRatio(S/N)
Parameterdescribingqualityofdata Oftenreferredtoasfigureofmerit Smeanofsignalx1 = = = NstandarddeviationsRSD RSD=relativestandarddeviation ImpossibletodetectasignalwhenS/Nlessthan2or3
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Very little confidence in ability to determine peaks at lower S/N Detection Limit
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SourcesofNoise
Chemicalnoise temp,pressure,humidity,etc. fluctuations=uncontrolledvariables Instrumentalnoise noise frominstrumental components
Thermalnoise(Johnsonnoise) thermalmotionof electronsinloadresistor Voltagefluctuation vrms =4kTRff =1/3tr Narrowbandwidthtodecreasenoise,butinstrument willbeslower
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Instrumentalnoise
Thermalnoise vrms =4kTRf vrms =rootmeansquarenoisevoltage k=Boltzmannconstant1.38x1023 J/K T=temperature R=resistance f=frequencybandwidthofnoise
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Instrumentalnoise
Shotnoise movementof electronsacrossajunction irms =2ief irms =rootmeansquarecurrentfluctuation i=averagecurrent e=chargeonelectron f=frequencybandwidth
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Instrumentalnoise
Flickernoise anynoise thatisinversely proportionaltosignal 1/f Significantatlowfrequency(<100Hz) Environmentalnoise compositeof manynoisesources e.g.anyelectricaldevicegivesoff EM(electromagneticradiation) ELFradiation=healthcontroversy instrumentsmaypickupsignals
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Environmentalnoisesources (notefrequencydependence)
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ImprovingS/N
hardware&software
Hardware
Grounding&shielding Faradaycage Analogfiltering RCfiltering Modulation convertDCsignaltohighfrequencyAC thendemodulate Signalchopping rotatingwheeltodifferentiatee.g.IR sourcefromheat Lockinamplifiers
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Primitive Faraday Cage for shielding instruments from EM Radiation must be grounded
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RC filter
R
Filtered data
C
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Modulation
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Signalchoppinginan IRspectrophotometer
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Chopperamplifier
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ImprovingS/N
hardware&software
Software
Ensembleaveraging addingspectra Boxcaraveraging Digitalfiltering movingwindow,slidingaverage Correlationmethods
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Boxcaraveraging
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