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BACTERIA
(bacterium) = relatively simple (Prok.) single-celled; unicellular organisms defined as mcgs considerably smaller than molds (fungi) Antoni van Leeuwenhoek (1676) -first person to see bacteria while studying (pepper) water infusion -reffered to them as wee animalcules
Bacteria
Robert Koch - first to grow bacteria on solid culture media (gelatin)
Cultural Characteristics
PROCEDURE.a
Pseudomonas aeruginosa Enterobacter sakazakii Micrococcus luteus
2/4/2012
PROCEDURE.A
NA slant
PROCEDURE.b
TZCA
unknown
YDCA KMBA NA
PLATES
PLATES
punctiform granular / spindle circular rhizoid
filamentous
irregular
2/4/2012
PLATES
PLATES
Figure 4. The relative growth requirements for oxygen. The medium without bacteria is yellow, while bacterial growth is indicated by the green color. For example, obligate aerobes and anaerobes, grow respectively only at the top (A) and bottom (D) of a tube of liquid medium. Obligate aerobes often form a film (scum) or pellicle, that floats on the top of the liquid. Facultative aerobes have the best of both worlds, as they are able to grow under both aerobic and anaerobic conditions (C). Microaerophilic bacteria require a little bit of oxygen, but too much is toxic (B).
2/4/2012
A B C D
KMBA
KMBA
Pure culture of P.fluorescens on Kings B medium under normal light B: Pure culture of P.fluorescens on Kings B medium under U.V light
2/4/2012
KMBA
Pseudomonas aeruginosa
FORM MARGIN ELEVATION Turbidity Sedimentation Pellicle/Ring irregular entire umbonate ++ +
TZCA
Enterobacter sakazakii
FORM punctiform MARGIN entire ELEVATION convex
+ + +
YDCA
Micrococcus luteus
FORM MARGIN ELEVATION circular entire convex
+; uniform +
2/4/2012
References
http://www.ispub.com/journal/the-internet-journal-of-veterinary-medicine/volume6-number-1/bio-morphological-characteristics-of-bacterial-species-identifiedfrom-mastitic-milk-samples-of-camel.html http://www.jstor.org/pss/30143814 http://www.aseanfood.info/Articles/11015335.pdf http://bioaliment.ugal.ro/revista/5/Paper%2053.pdf http://www.aseanfood.info/Articles/11015335.pdf http://www.slic2.wsu.edu:82/hurlbert/micro101/pages/Chap4.html Madigan, M.T., J.M. Martinko, D.A. Stahl, D.P.Clark. 2006. Brock Biology of Microorganisms. 13th ed. USA: Benjamin Cummings. pp. 133 and 145.