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Activity owner: Ritu Bhalla (Dr) Inputs: Ventris Dsouza (Dr) Approved by: Ventris DSouza (Dr) Module chair: Ritu Bhalla (Dr)
Todays Problem
A research company primarily focussing on identifying new potential drugs is expanding its research facilities in order to carry out cell culture assays. The management has identified premises where the new cell culture facility can be setup.
As a laboratory specialist, how would you assist your project director in converting the empty premises into a cell culture facility?
Copyright 2011 by Republic Polytechnic, Singapore
The Approach
What is cell culture? What are the special requirements (environment, equipment and materials) of a cell culture lab? What are the special techniques required for cell culture procedures? What are the procedures followed in a cell culture lab? How are the biosafety concerns addressed in a cell laboratory Why do we use cells in research? What are the areas of research where cell cultures are used ?
Copyright 2011 by Republic Polytechnic, Singapore
Biosafety cabinet
Sterilizer/ Autoclave
Incubator
Fridge/ Freezer
Haemocytometer
Centrifuge
Pipettes
Reagents
Media Supplements Antibiotics, Acids
Cells
Adherent cells Suspension cells
Copyright 2011 by Republic Polytechnic, Singapore
Click here to view a video on a typical cell culture laboratory layout and procedures
Copyright 2011 by Republic Polytechnic, Singapore
End of Activity 1
Copyright 2011 by Republic Polytechnic, Singapore