Agri Naturals

Amino Acid Supplement for plant growth promotion INTRODUCTION "EN-AMINO" is packed with the power of 17 natural L-Amino Acids with 35-40% content together with Folic Acid and trace element micronutrients. EN-AMINO is derived from 'soya' natural vegetable origin by process of enzymatic hydrolysis. Importance of Amino Acids as Foliar Spray to improve plant growth and crop output is acknowledged world wide. The amino acids get readily absorbed through leaves and aid in protein synthesis. It also leads to better synthesis of chlorophyll & helps to improve yield. In the ever changing environment, it strengths the immunity of plants to face insect attack, drought, frost etc. Also at the same time, amino acids are excellent food for microbial populations which produce organic acids in the soil that will lead to other benefits. Increased microbial population in the soil will produce organic acids that are the main agent responsible for dissolving minerals from insoluble complexes. These minerals are not available to plants under chemical fertilization regimes because they are water-soluble and the above mentioned minerals will not dissolve in water without organic acids. The exogenous application of amino acids reduces the energy requirements of plants and this saved energy can be used for vital processes, especially under extreme adverse conditions. The requirement of amino acids in essential quantities is well known as a means to increase yield, enhance immunity & overall quality of crops. USES Protein synthesis & uptake of Micro Nutrients. Increases photosynthesis as it contain glycine and prevents appearance of chlorosis. Enhances immunity & helps to withstand stress conditions. Induces flowering and fruit set related hormones Provides drought resistance by enhancing water holding capacity of the soil. Improves efficacy of the pesticides and fungicides. Reduce flower drop & fruit drop. Improves fruit size, quality & colour. Helps in increasing production Improve ability of roots for better absorption of nutrients. Contains high content of Glycine, praline, alanine and agrinine is an important source of Nitrogen which contribute to the saving of Nitrogen fertilizers. Greater uniformity in ripening

EN-AMINO

MECHANISM OF ACTION - Promotes uptake of macro and micro nutrients - Promotes enzymatic activity by acting as natural stimulant - Stimulates photosynthesis - Stimulates protein creation as it contains amino Acids which act as - building blocks - Induces flowering and fruit set related hormones - Improves the efficiency of the pesticides and fungicides - Provides drought resistance by its direct action

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Agri Naturals COMPOSITION OF EN-AMINO

AMINO ACID FOLIC ACID ORGANIC TRACE ELEMENTS EDTA Zinc EDTA FERRIC SODIUM EDTA Manganese EDTA MagnesSium EDTA Copper 40% W / W 0.2% W/W 0.75% W/W 0 .2 % 0 .2 % 0 .2 % 0 .2 % 0 .2 %

AMINO ACID COMPOSITION in EN-AMINO AVERAGE AMINO ACID COMPOSITION OF EN-AMINO (% of Total Amino Acids)
L Glutamic Acid L Aspartic Acid L Glycine L Leucine L Hydroxyproline L Isoleucine L Valine L Serine L Threonine L Phenylalanine L Alanine L Methionine L Tryphtophan L Arginine L Histidne L Cystine L Proline L Tyrosine 2.50 13.42 11.52 5.90 4.90 3.72 3.50 5.12 4.95 3.42 3.93 1.68 0.91 2.60 1.75 1.62 1.58 3.10

KEY ROLES OF MAJOR EN-AMINO Amino Acids are important protein building block crucial for plant metabolism. Each amino acid functions independently in such a manner that any slight excess or deficiency of every one of them can block the synthesis of the rest. EN-AMINO is standardized preparation formulated with balanced levels of essential Amino acids and other micronutrients. More over, Amino Acids directly or indirectly influence the physiological activities of the plant. Plants synthesize carbohydrates by photosynthesis; low photosynthesis rate implies a slow growth leading to death of the plant. Chlorophyll is responsible molecule for the absorption of the light energy. Glycine and Glutamic acid are fundamental metabolites in the process of formation of vegetable tissue and chlorophyll synthesis. These amino acids help to increase chlorophyll concentration in the plant leading to higher degree of photosynthesis. This makes crops lush green. L-proline helps in fertility of pollen. L-lysine, L-methionine, L-Glutamic acid are essential amino acids for pollination. Amino acids are precursors or activators of phytohormones and Growth URL: www.agrinaturals.com; E-mail: info@agrinaturals.com /agri@exoticnatural.com Skype /MSN: agrinaturals

Agri Naturals substances. L-methionine is precursor of ethylene and of Growth factors. Arginine induces flower / fruit related hormones, there improving flowering and fruiting formation. Valine plays a vital role in improving color and quality of produce. Proline acts on hydric balances thereby imparting stress tolerance and provides drought resistance. Aspartic acid helps in fruit hormone production while Histidine facilitates Nitrogen Metabolism.

WHY EN-AMINO? There are 2 types of process by which Amino acid can be obtained Enzymatic hydrolysis & acid hydrolysis. Only L-Amino acids (produced by enzymatic hydrolysis) are assimilated by plants. D-Amino acids (which are produced during acid hydrolysis) are not recognized by the enzymatic locus and therefore cannot participate in protein synthesis. One of the easiest parameter to identify the process by which the product is obtained is seeing the Ash content of the product. Higher the Ash means it contains undesired salts produced during the neutralization of acid hydrolyzed amino acids. EN-AMINO is prepared by enzymatic hydrolysis and contains no more than 5% ash which mainly comes from the pH adjustment for optimum utilization of combination of enzymes.

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Agri Naturals Further differences in Products obtained from different processes. Enzymatic Hydrolysis Carried out at a controlled Temperature and pressure at Optimum pH in presence of enzymes Consists of Twenty Amino Acids All amino acids are in L form (natural form) & are absorbed quickly & easily by plants.
No cyclization of glutamine which is important for energy metabolism. No destruction of aspargine which has active role in respiratory function. l-arginine induces synthesis of flower and fruit related hormones. Tryptophan which is starting material for synthesis of auxin is available in L form. L - tryptophan is precursor for Auxin synthesis. L- tryptophan is used in plants in L- form only. L-Tryptophan is available only if hydrolysis of protein is carried out by Enzyme. Serine & Threonine are free & in L form Aspartic acid & glutamic acid which are very important amino acids are available in free L form for easy absorption. Amide nitrogen is not formed High percentage of biological value & nutritive value. No inorganic nitrogen is present. Excess dose is non toxic.

Acid Hydrolysis Carried out at very Acidic pH and High temperature and pressure. Consists of sixteen to eighteen amino acids All amino acids are not in L-form, some are in d- form & hence cannot be absorbed easily.
Cyclization of glutamine. hence energy metabolism is affected Destruction of aspargine takes place which affects respiratory function Tryptophan is destroyed and auxin synthesis is affected

Serine & Threonine are partially decomposed. Aspartic acid & glutamic acid undergo changes and are not easily available for absorption. Amide nitrogen is formed No biological value & nutritive value is zero as Tryptophan, an essential amino acid is not present. Inorganic nitrogen is present as ammonium chloride. Excess dose is toxic.

SALIENT FEATURES OF EN-AMINO

Features EN-AMINO is prepared by Enzymatic hydrolysis. Natural vegetable origin Compatible with Pesticides Non Phytotoxic Biodegradable Benefits Balanced blend of L-Amino acids. Only L amino acids are assimilated by plants Safe in handling, no side effects Can be used simultaneously with other pesticides and insecticides excluding copper based products. Safe as foliar spray No residues in soil

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Agri Naturals FORMULATIONS & APPLICATION DETAILS Dosage : 1 1.25 liter per hectare Suggested Dilution : 1:200 in water Method of Usage : Foliar spray Recommended crops : Chilli, Paddy, Cotton, Sunflower, Onion, Tomato, Bringal, Okra, Vegetables, Grapes, Mango, Citrus. Suggested application details for some of the products as below: Sr.No Crop 1 Chillies 2 3 4 Sun Flower Paddy Cotton Application Time Per Bloom Stage Full Bloom Stage 30 Days after Sowing 45 Days after Sowing At tillering Stage 20 Days after I st Spray 40-50 Days after Sowing 65-70 Days after Sowing 85-90 Days after Sowing Pre Flowering Stage Peak - Flowering Stage 20-30 Days after Transplanting 15 Days after I st Spray Pre Flowering Peak Flowering Pre Flowering Peak Flowering to Fruit formation Stage 30,45,60 days after foundation and fruit pruning

5 6 7 8

Tomato Onion Okra Mango

Grapes

PRECAUTIONS & COMPATIBILITY DATA Compatibility - It can be used in combination insecticides, fungicides and plant nutrient. Precautions - EN-AMINO should not be mixed with copper base products. PRESENTATION Miscible liquid SHELF LIFE & STORAGE Shelf Life: 2 years

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Agri Naturals

EFFICACY DATA STUDY 1: EFFECTS OF EN-AMINO ON GROWTH AND YIELD OF CHILLI

Treatme nt No Treatments Green Chillies Yield/ ha

25

Green Chillies Yield/ha

T1

EN-AMINO 2.0 ml/lt at 50 DAT

18.92

20

15

T2

EN-AMINO 3 ml/lt at 50 DAT EN-AMINO 2.0ml/lt at 25 DAT and 1.0 ml/liter at 50 DAT

19.57

10

T3

20.26

T4 T5

EN-AMINO 3ml/lt split dose 1.5ml/lt at 25 DAT & 1.5 ml/lt at 24.70 50 DAT Water Spray at 50 DAT 17.98

0 T1 T2 T3 T4 T5

Treatment
T1 T2 T3 T4 T5

Detailed observation data for Effects of EN-AMINO on growth & Yield of Green chillies
Treatment No Plant Height (cm) No. of fruits/ plants Green Chillies Yield/ ha

Treatments

T1 T2 T3 T4 T5 SE+ CD at 5%

EN-AMINO 2.0 ml/liter at 50 DAT EN-AMINO 3 ml/liter at 50 DAT EN-AMINO 2.0ml/liter at 25 DAT and 1.0 ml/liter at 50 DAT EN-AMINO 3ml/liter split dose 1.5ml/liter at 25 DAT and 1.5 ml/liter at 50 DAT Water Spray at 50 DAT

47.20 50.80 49.29 55.83 47.03 1.00 3.09

152.15 179.03 163.80 214.75 149.55 3.61 11.11

18.92 19.57 20.26 24.70 17.98 0.45 1.39

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Agri Naturals STUDY 2: EFFECT OF THE EN-AMINO ON VARIOUS YIELDS ATTRIBUTES OF RICE
Effect of Dynamino on no. of Tillers / Rice Plant 10

Effect of Dynamino on Plant Height 98 97 Plant Height in cm

9.5

96 95 94 93 92 0 94 0.1 1.5 95 96 2 2.5 3 97

8.5

0.1 1.5

2.5

Series1 8.6 8.9 9.3 9.8 9.7 9.6 Dynamino (ml/lit of w ater)

Series1

97 97

Dynamino (ml/lit of w ater)

Effect of Dynamino on Grain Yield / Rice Panicle 20 Grain Yield / Rice Panicle (gm) 18 16 14 12 10 0 0.1 1.5 2 2.5 19 3 19

Series1 16 16

19 19

Dynamino (ml/lit of w ater)

Effect of Dynamino on no. of grains / rice panicle 88 86 84 82 80 78 76 74 72 0 0.1 1.5 2 2.5 3

Effect of Dynamino on Rice Grain Weight 19.4 Weight of 1000 grains (gm) 19.2 19 18.8 18.6 18.4 18.2 0 0.1 1.5 2 2.5 3

No. of Grains per Panicle

Series1 77.3 78.4 81.5 86.5 86.2 86.3 Dynamino (ml/lit of w ater)

Series1 18.6 19 19.2 19.4 19.3 19.3 Dynamino (ml/lit of w ater)

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Agri Naturals STUDY 3: SEED YIELD AND ANCILLARY CHARACTERS AS INFLUENCED BY ENAMINO IN SUNFLOWER HYBRID PKSH-1

Effect of Dynamino on Seed Yield Kg/ha 1000

Effect of Dynamino on Filled Seed of sunflow er 76 75 74 73 72 71 Series1

Seed Yield (Kg/ha)

800 700 600 500

0 813

750 892

1000 994

Filled seed (%)

900

0 72.8

750 75.7

1000 75.3

Series1

Dynamino (ml/ha)

Dynamino (ml/ha)

Effect of Dynamino on Oil Content 35.5

Effect of Dynamino of Seed Yield / plant 16

Seed Yield / plant (gm)

0 34.2 750 35.1 1000 32.9

35

Oil Content (%)

34.5 34 33.5 33 32.5 32 31.5

15 14 13 12 11 10 0 11.9 750 14.3 1000 15.3

Series1

Series1

Dynamino (ml/ha)

Dynamino (ml/ha)

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Agri Naturals
STUDY 4: PLANT HEIGHT, LEAF AREA, NO OF PODS,HUNDRED KERNEL WEIGHT AND PRODUCTION OF GROUNDNUT AS INFLUENCED BY DIFFERENT LEVELS OF EN-AMINO
Effect of Dynamino on Plant Height of Groundnut
37 Plant Height (cm) 36 35 34 33

Effect of Dynamino on Leaf Area (dm2/plant) 1.5 Leaf Area (dm2/plant) 1.4 1.3 1.2 1.1 1 0 0.5 0.75 1 1.5 2 2.5

0.5 0.7

1.5

2.5

Series1 34.6 34.9 35.1 35.7 36.7 35.8 35.4 Dynamino (ml/lit)

Series1 1.1 1.2 1.2 1.3

1.4 1.4 1.3

Dynamino (ml/lit) Effect of Dynamino on Groundnut Kernel Weight 40 Wt of hundred Kernel (gm)

Effect of Dynamino on no. of pods / plant 16 15 No. of Pods / Plant 14 13 12 11 10 0 0.5 0.7 1 1.5 2 2.5

35 30 25 20

0.5 0.7

1.5

2.5

Series1 9.5 11.9 12.9 14.5 15 13.6 13 Dynamino (ml/lit)

Series1 27.7 30.4 31.7 32.8 35.8 34.8 32.1

Dynamino (ml/lit)

Effect of Dynamino on Yield of Groundnut Pods

22 20 18 16 14 12 10
Yield of Pods (Q/ha)

0.5 0.7

1.5

2.5 19

Series1 15 18

18 18

20 19

Dynamino (ml/lit)

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Agri Naturals STUDY 5: EFFICACY OF EN-AMINO IN THE RETENTION OF BALLS AND YEILD OF COTTON Mean Balls/Plant 25.20 26.00 31.15 27.24 17.12 1.50 Mean Yield of Cotton in gm/plant 2052.5 2095.0 2357.4 2200.5 1805.0 92.6 Yield kg/ha 10.26 10.47 11.79 11.00 9.03 -Percent increase over control 13.62 15.94 30.561 21.81 ---

No. T1 T2 T3 T4 T5

Treatment EN-AMINO 1ml/lit water EN-AMINO 1.5ml/lit Water EN-AMINO 2 ml/lit water EN-AMINO 3 ml/lit water Control (untreated) CD at 5%

Effect of Dynamino on Cotton Yield

12 10
Yield kg/ha

8 6 4 2 0
T1 10.26 T2 10.47 T3 11.79 T4 11 T5 9.03

Series1

Treatment

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