HYPOGLYCEMIC ACTIVITY OF THE EXTRACT FROM THE SEED OF LANZONES (Lansium domesticum corr.

A Thesis Presented to The Faculty of the Department of Physical Sciences College of Science Polytechnic University of the Philippines Sta. Mesa, Manila

In Partial Fulfillment of the Requirements for the Degree Bachelor of Science in Chemistry By CABILLAN, CHERRY A. DEMETILLO, MECHEALLE E. FLORES, JASON C. PELAYO, SHIELA MARIE O. SABOLO, SARAH MARIE Z.

OCTOBER 2011
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APPROVAL SHEET

This thesis entitled Hypoglycemic Activity of the extract from the Seed of Lanzones (Lansium domesticum corr) prepared and submitted by Cherry A. Cabillan, Mechealle E. Demetillo, Jason C. Flores, Shiela Marie O. Pelayo and Sarah Marie Z. Sabolo, in partial fulfillment of the requirements for the degree Bachelor in Science, has been examined and recommended for acceptance and approval for Oral Examination.

PROFESSOR LORNA T. ENERVA Adviser Approved by the Panel of Examiners on Oral Examination.

MR. DALTON R. FORTIN Member, Oral Defense Panel

MS. EVELYN M.MATCHETE Member, Oral Defense Panel

OFELIA V. VILLAMOR Chair, Oral Defense Panel

Rating: ___________________

Date of Oral Defense: ______________

Accepted and approved in partial fulfillment of the requirements for the degree OF Bachelor of Science in Chemistry.

DR. EVELYN M.MATCHETE Chairperson, Department of Physical Sciences

DR. ZENAIDA R. SARMIENTO Dean, College of Science

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ACKNOWLEDGEMENT
This paper will not be accomplished without the help of some friends, professors and foes who give time, effort and help for the succession of this thesis. This paper also gives honor to them for their contribution on our paper. First of all, we would like to thank Prof. Eunice Biteng for allowing us to use this topic for our thesis. Also for her warm and generous help in discussion of this paper and sharing of ideas, a massive thank you madam. Second, we like to give a huge thank you to Mr. Jeffrey Maylas who give us the opportunity to use their Rotavap equipment to obtained extracts and share his knowledge towards experimentation. Another we would like to extend our deepest gratitude to Mr. Welvin for giving us the opportunity to use their laboratory and assisting us in our bioassay using mice And Ms. Faye for letting us occupy the storage area and store some of our things. We as well want to give a big thank you for Mr. Irving Chiong and the one and only Instructor Aldrin Trance for the interpretation and discussion of our spectral data. Instructor Aldrin was an angel given by the heaven in our study because he also taught us techniques to use in our experimentation. A big round of a plause for you sir from all of us! Also, thank you for the support and part of our dearest classmates, Gladys, DTE especially Sherwin and Jose Mari Felicita for their help, and most important of all to our beloved family and our Lord God for the good vibes and positive outlook towards the heavy pressure of this thesis because without them this thesis will not succeed.
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ABSTRACT

Lanzones (lansium Domesticum corr.) Seed exhibits hypoglycemic activity. The obtained extract undergo different chemical at physical analysis. The bioactive components were isolated using Thin Layer Chromatography and column chromatography and characterized using Solvent-solvent extraction. To further characterized the bioactive active components the sample were subjected to instrumental analysis using Gas chromatography Mass Spectroscopy and Infrared Spectroscopy. The concentrated extract was paired with different the solvent and nbutanol extract emerge having the highest hypoglycemic activity towards them. The presence of compound such as Methanoazulene, Germacrene, Naphthalene, Preg4-en-3-one and Hexadecanoic acid contribute on the activity of the Lanzones extract because of their high concentration and activity in the sample based on the Gas chromatography Mass spectroscopy data. The determination of active biocomponents in Lansium domesticum seed extract can help contribute to the growing study of hyperglycemia in the Philippines.

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TABLE OF CONTENTS TITLE PAGE ......... i APPROVAL SHEET ........ ii ACKNOWLEDGEMENT . iii ABSTRACT ... iv TABLE OF CONTENTS ......... v LIST OF TABLES .... vii LIST OF FIGURES . viii CHAPTER 1: INTRODUCTION ..... 1 1.1 Objectives of the study ..... 2 1.2 Significance of the study ...... 3 1.3 Scope and limitation ......... 3 1.4 Definition of Terms ....... 4 CHAPTER 2 REVIEW OF RELATED LITERATURE .... 6 2.1 Related Literature ..... 6 CHAPTER 3 METHODOLOGY .........18 3.1 General outline ........... 18 3.2 Materials and Methods .. 19 3.3 Sample Preparation 19 3.4 Purification and isolation ....... 20
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3.5 Chemical analysis ... 21 3.6 Bioassay .... 21 3.7 Instrumental analysis. ..... 24 CHAPTER 4 RESULTS AND DISCUSSION ..... 26 4.1 Bioassay ............... 26 4.2 Purification of compounds ......... 29 4.3 Dragendorff and Mayers Tests ........ 30 4.4 Gas Chromatography-Mass Spectroscopy analysis. 30 4.5 Infrared spectroscopy analysis ..... 38 CHAPTER 5 CONCLUSION AND RECOMMENDATION .. 40 5.1 Conclusion ... 40 5.2 Recommendation. 41 REFERENCES ... 42 APPENDICES ...45 Tables and figures .......46 Sample Computation .... 73 Picture Gallery .....75 Curriculum Vitae .... 79 Certificates ...... 85

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LISTS OF TABLES

1. Table 4.1 Blood Glucose reducing potential of Lanzones (Lansiumdomesticum) 2. Table 4.2 Data result of the Rf values of the fractions of the samples 3. Table 4.3 Summary of bioactive Compounds charactesized in GC-MS 4. Table 4.4. Detected functional groups in IR Spectra

LIST OF FIGURES
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1. Figure 4.1 shows the results for the blood glucose reducing potential of Lanzones (Lansium domesticum) as a function of time. 2. Figure 4.2 Blood Glucose reducing potential of Lanzones (Lansium domesticum) of different extract at 60 and 90 minutes 3. Figure 4.3 Blood Glucose reducing potential of Lanzones (Lansium domesticum) Of different extract 4. Figure 4.4. General Spectra from time 12.99 to 17.99 this shows that Lansones seed oil scan from electron 5. Figure 4.5: 1,6-Cyclodecadiene, 1-methyl-5-methylene-8-(1-methylethyl)-, [s(E,E)]-5. GC-MS spectra at 9.28 minutes 6. Figure 4.6: 1H-3a,7-Methanoazulene / Patchoulane GC-MS spectra 7. Figure 4.7 Naphthalene GC-MS spectra 8. Figure 4.8:, 17-hydroxy-17-cyano- Preg-4-en-3-one GC-MS spectra 9. Figure 4.: 9Hexadecanoic acid GC-MS spectra at 1.03 minutes 10. Figure 4.10 Acetic acid, butyl ester GC-MS spectra 11. Figure 4.11: 2-methyl-1-nitro-Propane GC-MS spectra 12. 7. Figure 4.12 :3-Methyl-4-heptanone GC-MS spectra 13. Figure 4.13: Butanoic acid GC-MS spectra 14. Figure 4.14: Hexanoic acid GC-MS spectra at 8.59 minutes 15. Figure 4.15:.Butyl butanoate GC-MS spectra 16. Figure 4.16. General Spectra from time 7.0 to 12.0 this shows that Lanzones seed oil scan from electron 17. Figure 4.17: 1,1'-[ethylidenebis(oxy)]bis- Butane GC-MS spectra 18. Figure 4.18: Octadecanoic acid GC-MS spectra 19. Figure 4.19: 4H-Pyran-4-one GC-MS spectra 20. Figure 4.20: 2,2'-[methylenebis(oxy)]bis[2-methyl-propane GC-MS spectra
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21. Figure 4.21: Ethyl hydrogen succinate GC-MS spectra 22. Figure 4.22: Methyl salicylate GC-MS spectra 23. Figure 4.23: Phenol, 4-ethenyl-, acetate GC-MS spectra 24. Figure 4.24: 1,1-dibutoxy- Butane GC-MS spectra 25. Figure 4.25: 1-methyl-5-methylene-8-(1-methylethyl)-, [s-(E,E)]- 1,6Cyclodecadiene / Germacrene D GC-MS spectra at 6.40 minutes

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