BC-2800Vet Operation Manual (v1.8)

BC-2800Vet
Auto Hematology Analyzer
Operators Manual
2005-2009 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved. For this Operators Manual, the issued date is 2009-06.
Intellectual Property Statement

SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called Mindray) owns the intellectual property rights to this Mindray product and this manual. This manual may refer to information protected by copyright or patents and does not convey any license under the patent rights or copyright of Mindray, or of others. Mindray intends to maintain the contents of this manual as confidential information. Disclosure of the information in this manual in any manner whatsoever without the written permission of Mindray is strictly forbidden. Release, amendment, reproduction, distribution, rental, adaptation, translation or any other derivative work of this manual in any manner whatsoever without the written permission of Mindray is strictly forbidden.
are the trademarks, registered or otherwise, of Mindray in China and
other countries. All other trademarks that appear in this manual are used only for informational or editorial purposes. They are the property of their respective owners.
Responsibility on the Manufacturer Party

Contents of this manual are subject to change without prior notice.
All information contained in this manual is believed to be correct. Mindray shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this manual.
Mindray is responsible for the effects on safety, reliability and performance of this product, only if: all installation operations, expansions, changes, modifications and repairs of this product are conducted by Mindray authorized personnel; the electrical installation of the relevant room complies with the applicable national and local requirements; and the product is used in accordance with the instructions for use. I
It is important for the hospital or organization that employs this equipment to carry out a reasonable service/maintenance plan. Neglect of this may result in machine breakdown or personal injury. Be sure to operate the analyzer under the situation specified in this manual; otherwise, the analyzer will not work normally and the analysis results will be unreliable, which would damage the analyzer components and cause personal injury.
This equipment must be operated by skilled/trained clinical professionals.
II
Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE.
Definitions
Main unit: Integrated facilities which implement the specified function separately. Generally speaking, the main unit should include power supply, control system and some functional modules. Accessories: Materials connected to the main unit to extend or implement specified function. Consumables: Disposable or short-life parts which should be replaced each time after use or periodically
Exemptions
Mindray's obligation or liability under this warranty does not include any transportation or other charges or liability for direct, indirect or consequential damages or delay resulting from the improper use or application of the product or the use of parts or accessories not approved by Mindray or repairs by people other than Mindray authorized personnel.
This warranty shall not extend to: Malfunction or damage caused by improper use or man-made failure. Malfunction or damage caused by unstable or out-of-range power input. Malfunction or damage caused by force majeure such as fire and earthquake. Malfunction or damage caused by improper operation or repair by unqualified or unauthorized service people. Malfunction of the instrument or part whose serial number is not legible enough. Others not caused by instrument or part itself.
The standard warranty period is as below: Main unit: 18 months from shipment Accessories: 15 months from shipment Consumables: N/A
III
Return Policy
Return Procedure
In the event that it becomes necessary to return this product or part of this product to Mindray, the following procedure should be followed: 1. Return authorization: Contact the international Customer Service Department and obtain a Return Materials Authorization number. This number must appear on the outside of the shipping container. Returned shipments will not be accepted if the number is not clearly visible. Please provide the model number, serial number, and a brief description of the reason for return. 2. Freight policy: The customer is responsible for freight charges when this product is shipped to Mindray for service (this includes customs charges). 3. Return address: Please send the part(s) or equipment to the address offered by the international Customer Service Department.
Company Contact
Manufacturer: E-mail Address: Tel: Fax: Shenzhen Mindray Bio-Medical Electronics Co., Ltd. service@mindray.com.cn +86 755 26582479 26582888 +86 755 26582934 26582500
EC-Representative: Address: Tel: Fax:
Shanghai International Holding Corp. GmbH(Europe) Eiffestrae 80, Hamburg 20537, Germany 0049-40-2513175 0049-40-255726
IV
Table of Contents
1 Using This Manual ................................................................................... 1-1 1.1 Introduction ............................................................................................ 1-1 1.2 Who Should Read This Manual ............................................................. 1-2 1.3 How to Find Information......................................................................... 1-3 1.4 Conventions Used in This Manual ......................................................... 1-4 1.5 Special Terms Used in This Manual....................................................... 1-5 1.6 Symbols ................................................................................................. 1-6 Understanding Your Analyzer................................................................. 2-1 2.1 Introduction ............................................................................................ 2-1 2.2 Intended Use.......................................................................................... 2-2 2.3 User Interface......................................................................................... 2-4 2.3.1 LCD ............................................................................................. 2-7 2.3.2 Input Devices .............................................................................. 2-7 2.3.3 Recorder...................................................................................... 2-9 2.3.4 Keyboard Interface...................................................................... 2-9 2.3.5 Serial Ports.................................................................................. 2-9 2.3.6 Parallel Port................................................................................. 2-9 2.3.7 Power Supply for the Floppy Disk Drive ..................................... 2-9 2.3.8 Power Indicator ........................................................................... 2-9 2.3.9 Optional Devices ......................................................................... 2-9 2.4 Instrument Software ............................................................................. 2-10 2.4.1 Main Screen .............................................................................. 2-10 2.4.2 Screen saver ............................................................................. 2-11 2.4.3 System Menu ............................................................................ 2-12 2.5 Reagents, Controls and Calibrators..................................................... 2-14 2.5.1 Diluent ....................................................................................... 2-14 2.5.2 Lyse ........................................................................................... 2-15 2.5.3 Rinse ......................................................................................... 2-15 2.5.4 E-Z Cleanser ............................................................................. 2-15 2.5.5 Probe Cleanser ......................................................................... 2-15 2.5.6 Controls and Calibrators ........................................................... 2-15 Understanding the System Principles ................................................... 3-1 3.1 Introduction ............................................................................................ 3-1 3.2 Aspiration ............................................................................................... 3-2 3.3 Dilution ................................................................................................... 3-3
1
Table of Contents 3.4 WBC/HGB Measurement....................................................................... 3-4 3.4.1 Volumetric Metering .................................................................... 3-4 3.4.2 Measurement Principles.............................................................. 3-5 3.4.3 Derivation of WBC-Related Parameters ..................................... 3-6 3.4.4 HGB............................................................................................. 3-7 3.5 RBC/PLT Measurement ......................................................................... 3-8 3.5.1 Volumetric Metering .................................................................... 3-8 3.5.2 Measurement Principles.............................................................. 3-9 3.5.3 Derivation of RBC-Related Parameters ...................................... 3-9 3.5.4 Derivation of PLT-Related Parameters ..................................... 3-10 3.6 Wash .................................................................................................... 3-12
Installing Your Analyzer .......................................................................... 4-1 4.1 Introduction ............................................................................................ 4-1 4.2 Installation Requirements....................................................................... 4-2 4.2.1 Space Requirements................................................................... 4-2 4.2.2 Power Requirements................................................................... 4-2 4.2.3 General Environment .................................................................. 4-3 4.3 Unpacking .............................................................................................. 4-4 4.3.1 Unpacking and Inspecting the Analyzer...................................... 4-4 4.3.2 How to move the analyzer........................................................... 4-4 4.4 Installation Procedure ............................................................................ 4-5 4.4.1 Connecting Regents.................................................................... 4-5 4.4.2 Installing Recorder Paper............................................................ 4-7 4.4.3 Connecting the Keyboard............................................................ 4-8 4.4.4 Connecting the Printer (Optional) ............................................... 4-9 4.4.5 Connecting the Bar-Code Scanner (Optional) ............................ 4-9 4.5 Starting the Analyzer ............................................................................ 4-10 Customizing the Analyzer Software ....................................................... 5-1 Introduction ............................................................................................ 5-1 5.2 Password ............................................................................................... 5-2 5.2.1 Entering the administrator password .......................................... 5-2 5.2.2 Resuming the common user password....................................... 5-3 5.3 Editing Settings ...................................................................................... 5-4 5.3.1 Reagent....................................................................................... 5-5 5.3.2 Printing and Communication Settings......................................... 5-7 5.3.3 Date and Time........................................................................... 5-11 5.3.4 Gain........................................................................................... 5-13 5.3.5 Count......................................................................................... 5-16 5.3.6 Reference Range ...................................................................... 5-19 5.3.7 Other Settings ........................................................................... 5-21 5.3.8 Special Fluidic Settings (for user-defined animals)................... 5-24
5.1
Table of Contents
Operating Your Analyzer ......................................................................... 6-1 6.1 Introduction ............................................................................................ 6-1 6.2 Initial Checks.......................................................................................... 6-2 6.3 Power-on................................................................................................ 6-3 6.4 Daily Quality Control .............................................................................. 6-4 6.5 Selecting Animal .................................................................................... 6-5 6.6 Define an animal .................................................................................... 6-6 6.7 Sample Collection and Handling............................................................ 6-7 6.7.1 Whole Blood Samples................................................................. 6-7 6.7.2 Prediluted Samples ..................................................................... 6-7 6.8 Running Whole Blood Samples ........................................................... 6-10 6.8.1 Entering Sample Information .................................................... 6-10 6.8.2 Running the Samples................................................................ 6-14 6.8.3 Special Functions ...................................................................... 6-15 6.9 Running Prediluted Samples ............................................................... 6-17 6.9.1 Entering Sample Information .................................................... 6-17 6.9.2 Running the Samples................................................................ 6-21 6.9.3 Special Functions ...................................................................... 6-22 6.10 Shutdown ............................................................................................. 6-24 Reviewing Sample Results ..................................................................... 7-1 7.1 Introduction ............................................................................................ 7-1 7.2 Browsing All Sample Results ................................................................. 7-2 7.2.1 Browsing in the Histogram Mode ................................................ 7-2 7.2.2 Browsing in the Table mode...................................................... 7-4 7.3 Searching for Specific Sample Results ................................................ 7-11 7.3.1 Starting a search ....................................................................... 7-11 7.3.2 Reviewing Search Result in the Table Mode .......................... 7-12 7.3.3 Reviewing Search Result in the Histogram Mode .................. 7-19 Using the QC Programs .......................................................................... 8-1 8.1 Introduction ............................................................................................ 8-1 8.2 QC Editing.............................................................................................. 8-2 8.2.1 Entering the Quality control screen .......................................... 8-2 8.2.2 Selecting a QC File ..................................................................... 8-2 8.2.3 Editing L-J Settings ..................................................................... 8-3 8.3 Running the Controls ............................................................................. 8-6 8.3.1 Whole Blood Mode...................................................................... 8-6 8.3.2 Prediluted Mode .......................................................................... 8-7 8.4 Reviewing QC Results ......................................................................... 8-10 8.4.1 L-J Graph .................................................................................. 8-10 8.4.2 QC Table ................................................................................... 8-12 Using the Calibration Programs ............................................................. 9-1
3
Table of Contents 9.1 9.2 9.3 Introduction ............................................................................................ 9-1 When to calibrate ................................................................................... 9-2 How to Calibrate..................................................................................... 9-3 9.3.1 Preparing Your Analyzer.............................................................. 9-3 9.3.2 Auto Calibration Program............................................................ 9-4 9.3.3 Manual Calibration Program ..................................................... 9-10
10
Maintaining Your Analyzer .................................................................... 10-1 10.1 Introduction .......................................................................................... 10-1 10.2 General Guidelines .............................................................................. 10-2 10.3 Using the Maintenance Program ....................................................... 10-3 10.3.1 Diluent Prime............................................................................. 10-4 10.3.2 Rinse Prime............................................................................... 10-5 10.3.3 Lyse Prime ................................................................................ 10-6 10.3.4 Zap Aperture ............................................................................. 10-7 10.3.5 Flush Aperture........................................................................... 10-8 10.3.6 Probe Cleanser Cleaning .......................................................... 10-9 10.3.7 E-Z Cleanser Cleaning............................................................ 10-11 10.3.8 Lyse Test ................................................................................. 10-13 10.3.9 Clean Bath .............................................................................. 10-15 10.3.10 Drain Bath............................................................................. 10-16 10.3.11 Drain Tubing ......................................................................... 10-18 10.3.12 Clean Wipe Block ................................................................. 10-19 10.3.13 Prepare to ship ..................................................................... 10-21 10.4 Using the Status Program................................................................ 10-23 10.5 Using the Self-test Program ............................................................ 10-24 10.5.1 Testing the fluidic system ........................................................ 10-25 10.5.2 Testing Motors and Recorder/Printer ...................................... 10-25 10.5.3 Testing Valves ......................................................................... 10-26 10.5.4 Testing A/D Interrupt................................................................ 10-27 10.6 Log ..................................................................................................... 10-28 10.7 Viewing System Configuration ........................................................... 10-30 10.8 Printing Management......................................................................... 10-31 10.9 Calibrating Sample Probe Position .................................................... 10-32 10.10 Replacing the Probe Wipe ................................................................. 10-36 10.11 Replacing the Filter of the Vacuum Chamber .................................... 10-37 10.12 Maintaining Recorder ......................................................................... 10-39 Troubleshooting Your Analyzer ............................................................ 11-1 11.1 Introduction .......................................................................................... 11-1 11.2 Errors without available error messages ............................................. 11-2 11.3 Errors indicated by error messages ..................................................... 11-3 Appendices ..............................................................................................A-1
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11
12
Table of Contents
A B C D
Index .........................................................................................................A-1 Specifications ..........................................................................................B-1 Precautions, Limitations and Hazards ...................................................C-1 Communication .......................................................................................D-1
1
1.1 Introduction
Using This Manual
This chapter explains how to use your Auto Hematology Analyzer operators manual, which is shipped with your BC-2800Vet auto hematology analyzer and contains reference information about the BC-2800Vet and procedures for operating, troubleshooting and maintaining the analyzer. Read this manual carefully before operating your analyzer and operate your analyzer strictly as instructed in this manual.
Operate your analyzer strictly as instructed in this manual. All illustrations in this manual are provided as examples only. They may not necessarily reflect your analyzer setup or data displayed and must not be used for any other purpose.
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Using This Manual
1.2 Who Should Read This Manual

This manual contains information written for clinical laboratory professionals to learn about the BC-2800Vet hardware and software; customize system settings; perform daily operating tasks; perform system maintenance and troubleshooting.
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Using This Manual
1.3 How to Find Information

This operators manual comprises 11 chapters and 4 appendices. Refer to the table below to find the information you need. If you want to learn about the intended use and parameters of the BC-2800Vet learn about the hardware and software of the BC-2800Vet See Chapter 2 Understanding
Your Analyzer Chapter 2 Understanding
Your Analyzer learn about how the BC-2800Vet works Chapter 3 Understanding the System Principles learn about how to install the BC-2800Vet Chapter Analyzer learn about how to define/adjust system settings Chapter 5 Customizing the Analyzer Software learn about how to use the BC-2800Vet to perform your daily operating tasks learn about how to review the saved analysis results Chapter 6 Operating Your Analyzer Chapter 7 Reviewing Sample Results learn about how to use the quality control programs Chapter Programs learn about how to calibrate the BC-2800Vet Chapter 9 Using the 8 Using the QC 4 Installing Your
Calibration Programs learn about how to maintain/service the BC-2800Vet Chapter 10 Maintaining Your Analyzer learn about the meanings of the error messages and how to correct the problems learn about the technical specifications of the BC-2800Vet see the summary of all safety messages included in this manual learn about the communication protocol of the BC-2800Vet Chapter 11 Troubleshooting Your Analyzer Appendix B Specifications Appendix C Precautions,
Limitations and Hazards Appendix D Communication
1-3
Using This Manual
1.4 Conventions Used in This Manual

This manual uses certain typographical conventions to clarify meaning in the text: All capital letters enclosed in [ ] indicate a key name (either on the built-in keypad or the external keyboard), such as [ENTER]. All capital, bold and italic letters indicate a special operation defined in the following section, such as SELECT. Bold letters included in indicate text you can find on the screen, such as Prepare to ship. Bold letters indicate defined screen areas/fields, such as System Status area, or chapter titles, such as Chapter 1 Using This Manual. All illustrations in this manual are provided as examples only. They may not necessarily reflect your analyzer setup or data displayed.
1-4
Using This Manual
1.5 Special Terms Used in This Manual

When you read CLICK It means to press the arrow keys ([][] [][]) as needed to move the cursor to a certain software button on screen and press [ENTER]. to press the arrow keys ([][] [][]) as needed to move cursor to the desired edit box and use the built-in keypad or the external keyboard to enter the desired characters or digits. ENTER Note that besides the numeric keys you may also use the [PgUp] or [PgDn] keys to enter digits; or to scan the number in using the bar-code scanner. to press the arrow keys ([][] [][]) as needed to move the cursor to the character or digit to the left of the one you want to delete and press [DEL]; or to press the arrow keys DELETE ([][][][]) as needed to move the cursor to the character or digit to the right of the one you want to delete and press [BackSpace] on the external keyboard. to move the cursor to the character or digit you want to change MODIFY and re-enter the desired one using either the built-in keypad or the external keyboard. to press the arrow keys ([][] [][]) as needed to move the SELECT from ** pull-down list cursor to the desired edit box and press [ENTER] to display the pull-down list and press [] or [] to move the cursor to the desired item and press [ENTER] to select it. to press the arrow keys ([][] [][]) as needed to the SELECT desired item and press [ENTER].
This analyzer adopts a fixed decimal point. You can enter the digits without bothering to look for the [.] on the external keyboard.
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Using This Manual
1.6 Symbols
You will find the following symbols in this manual. When you see Then read the statement below the symbol. The statement is alerting you to an operating hazard that can cause personnel injury. read the statement below the symbol. The statement is alerting you to a possibility of analyzer damage or unreliable analysis results. read the statement below the symbol. The statement is alerting you to information that requires your attention. read the statement below the symbol . The statement is alerting you to a potentially biohazardous condition.
You may find the following symbols on the analyzer or the reagents. When you see It means EQUIPOTENTIALITY
CAUTION,
CONSULT
ACCOMPANYING
DOCUMENTS. BIOLOGICAL RISK
HIGH VOLTAGE
ALTERNATING CURRENT
USE BY
1-6
Using This Manual SERIAL NUMBER
DATE OF MANUFACTURE
TEMPERATURE LIMITATION
CONSULT INSTRUCTIONS FOR USE
THE DEVICE IS FULLY CONFORMANCE WITH THE COUNCIL EC LOW DIRECTIVE VOLTAGE AND EC
CONCERNING DIRECTIVE DIRECTIVE
(73/23/EEC) OF
ELECTROMAGNETIC
COMPATIBILITY (2004/108/EC). THE LABEL APPLIES TO U.S.A ONLY: FEDERAL LAW RESTRICTS THIS DEVICE TO SALE BY OR ON THE ORDER OF A VETERINARIAN. MANUFACTURER
AUTHORISED REPRESENTATIVE IN THE EUROPEAN COMMUNITY IRRITATING SUBSTANCE
THE FOLLOWING DEFINITION OF THE WEEE LABEL APPLIES TO EU MEMBER STATES ONLY: THE USE OF THIS SYMBOL INDICATES THAT THIS PRODUCT SHOULD NOT BE TREATED BY AS HOUSEHOLD THAT THIS
WASTE.
ENSURING
PRODUCT IS DISPOSED OF CORRECTLY, YOU WILL HELP PREVENT BRINGING
1-7
Using This Manual POTENTIAL NEGATIVE CONSEQUENCES TO THE ENVIRONMENT AND HUMAN HEALTH. FOR MORE WITH AND PLEASE FROM DETAILED TO THIS THE YOU
INFORMATION RETURNING PRODUCT,
REGARD
RECYCLING CONSULT WHOM
DISTRIBUTOR
PURCHASED THE PRODUCT.
1-8
Using This Manual
3 2
Figure 1-1 Back of the analyzer
(1) Equipotentiality.
2 Connect only to a properly earth grounded outlet; To avoid electric shock, disconnect power cord prior to removing or replacing fuse; Replace fuse only with the type and rating specified.
3 Biological risk.
4 The following definition of the WEEE label applies to EU member states only: The use of this symbol indicates that this product should not be treated as household waste. By ensuring that 1-9
Using This Manual this product is disposed of correctly, you will help prevent bringing potential negative consequences to the environment and human health. For more detailed information with regard to returning and recycling this product, please consult the distributor from whom you purchased the product.
Figure 1-2 Warning label
5
To avoid being injured, do not put hand under the motor when the machine is running.
1-10
Using This Manual
Figure 1-3 Warning label
6 To avoid electrical shock, disconnect the power supply before maintaining this device.
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Using This Manual
Figure 1-4 warning label
7 High Voltage
1-12
Understanding Your Analyzer
2.1 Introduction
The BC-2800Vet Auto Hematology Analyzer is a quantitative, automated hematology analyzer and leukocyte differential counter for Diagnostic Use in veterinary laboratories.
2-1
2.2 Intended Use
The purpose of this analyzer is to identify the normal animal patient, with all normal system-generated parameters, and to flag or identify patient results that require additional studies.
The analyzer is used for the quantitative determination of a maximum of 18 parameters and 3 histograms of blood samples. See Table 2-1 for details. White Blood Cell or leukocyte Lymphocyte Monocyte Granulocyte Lymphocyte percentage Monocyte percentage Granulocyte percentage Red Blood Cell or erythrocyte Hemoglobin Concentration Mean Corpuscular (erythrocyte) Volume Mean Cell (erythrocyte) Hemoglobin Mean Cell (erythrocyte) Hemoglobin Concentration Red Blood Cell (erythrocyte) Distribution Width Hematocrit Platelet Mean Platelet Volume Platelet Distribution Width Plateletcrit WBC Lymph# Mon# Gran# Lymph% Mon% Gran% RBC HGB MCV MCH MCHC RDW HCT PLT MPV PDW PCT
White Blood Cell Histogram Red Blood Cell Histogram Platelet Histogram
WBC Histogram RBC Histogram PLT Histogram
2-2
Table 2-1 Parameters and animals

Parameter Dog Cat Horse Rat Mouse Rabbit Monkey Cow Pig Buffalo Sheep Camel Goat Userdefined
WBC Lymph# Mon# Gran# Lymph% Mon% Gran% RBC HGB MCV MCH MCHC RDW HCT PLT MPV PDW PCT WBC Histogram RBC Histogram PLT Histogram
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2.3 User Interface
Figure 2-1 Front view
1 ---- LCD 3 ---- Recorder 5 ---- Aspirate key
2 ---- Keypad 4 ---- Power indicator 6 ---- Sample probe
2-4
Figure 2-2 Back view
1 --- Power switch 3 --- Waste outlet(Red) 5 --- Rinse inlet(Blue)
2 --- Equipotentiality 4 --- Lyse inlet(Orange) 6 --- Diluent inlet(Green)
2-5
Figure 2-3 Left view
1 --- RS-232 port1 3 --- RS-232 port2 5 --- Keyboard interface
2 --- Parallel port 4 --- Power Interface of Floppy Disk Drive
2-6
2.3.1 LCD
The LCD is located on the front panel of the analyzer, as Figure 2-4 shows. It displays all alphanumeric and graphic data.
2.3.2 Input Devices

The input devices include the aspirate key, built-in keypad and PS/2 keyboard. Aspirate key The aspirate key is located behind the sample probe, as Figure 2-4 shows. You can press the key to start the selected run cycle or dispense diluent.
Figure 2-4 Sample probe and aspirate key
1.Sample Probe
2. Aspirate Key
2-7
Built-in keypad The 18-key keypad is located below the LCD, as Figure 2-5 shows.
Figure 2-5 Build-in keypad PS/2 keyboard The analyzer can also be controlled by an external PS/2 keyboard that should be connected to the analyzers keyboard interface. See Table 2-2 Key functions for the correspondence between the keypad keys and the keyboard keys and for their functions. Table 2-2 Key functions Keypad [MENU] [PRINT] [HELP] [DEL] [ENTER] [], [], [], [] [F1], [F2], [F3], [F4], [F5] [PgUp][PgDn] PS/2 keyboard [Esc] [P] or [p] [H] or [h] [Delete] or [Del] [Enter] [], [], [], [] [F1], [F2], [F3], [F4], [F5] [PageUp] [PageDown] [MODE] [Ctrl+A] Switch to another analysis mode (works only in the Count screen). [DILUENT] In the prediluted mode, press it if you want to dispense diluent into the sample tube. / Other keys Use them as needed. See the rest of this manual or the analyzers on-line help for details. Press them to use various functions. See the rest of this manual or the analyzers on-line help for details. Press them to scroll the screen. Function Press it to enter/exit the system menu. Press it to print out data by the recorder or printer. Press it to call out the analyzers on-line help. Press it to delete data and characters. Press it to confirm or execute an operation Press them to move the cursor.
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2.3.3 Recorder
A thermal recorder is located on the front panel. It prints out analysis reports and other related information.
2.3.4 Keyboard Interface

A PS/2 keyboard can be connected here.
2.3.5 Serial Ports

The analyzer provides two RS-232 ports, one for connecting the scanner and the other for connecting a computer (host).
2.3.6 Parallel Port

The analyzer provides a parallel port to connect a printer or a floppy disk drive (a floppy disk drive is needed to upgrade the system software; the drive can only be connected by a Mindray-supplied cable).
2.3.7 Power Supply for the Floppy Disk Drive

It supplies power to the connected floppy disk drive. Only the drive power cable supplied by Mindraycan be used.
2.3.8 Power Indicator

The power indicator tells you whether the analyzer is on, off or in the screen saver mode.
2.3.9 Optional Devices

Printer An external printer can be connected to the parallel port at the left side of the analyzer. You can use it to print out a detailed report and other related information. Bar-code scanner A bar-code scanner can be connected to the RS-232 port 1 of the analyzer. You can use it to scan the bar-coded sample IDs into the analyzer.
Use the printer and scanner of the specified model.
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2.4 Instrument Software

2.4.1 Main Screen
After finishing the startup procedure, the analyzer enters the Count screen, which is the screen to be used most frequently, hence the name Main screen, as Figure 2-6 shows. Title Area Count Mode Area System Status Area
Error Message Area Title Area
Help Area
Reagent Status Area
Figure 2-6 Count screen
The Title area displays the title of the current screen. Count Mode Area The Count Mode area displays in which analysis (count) mode, whole blood or prediluted, the next sample is to be analyzed. System Status Area The System Status area displays whether this analyzer is ready for the next analysis. When it displays Ready, it means this analyzer is ready and you can proceed to analyze the next sample. When it displays Waiting, it means the analyzer is not ready for the next run yet. When it displays Running, it means this analyzer is analyzing a sample. System Time Area
2-10
Understanding Your Analyzer The System Time area displays the system time. Sample Information Area The Sample Information area has two sub-areas, the upper titled Current sample and the lower Next sample. The Current sample refers to the sample, whose analysis result is displayed on the Count screen. The animal information (type, name, gender, age), sample ID, time of analysis, mode and owners name are respectively displayed in the fields of the Current sample area. The Next Sample refers to the sample to be analyzed next. Its sample ID is displayed in the Next sample area. Analysis Result Area The Analysis Result area displays the analysis result. Error Message Area The Error Message area displays error messages one by one, alternating every two seconds. Reagent Status Area The Reagents Status area displays how many counts the remaining reagents are enough for. Note that when it displays 300 counts, it indicates the reagents are enough for over 300 counts and there is also enough space left in the waste container for the counts; when it displays 0 counts, it indicates either at least one of the reagents is insufficient or the waste container is full. Menu Area When you press [MENU], this area displays the system menu. Help Area The Help area reminds you how to proceed to the next step.
2.4.2 Screen saver

This analyzer will enter the screen saver if it has been idle at the Count screen for 10 minutes. When it happens, the sample probe will retract into the analyzer, the LCD will turn dark and the power indicator will be flickering. You can press any key to resume the display and reposition the sample probe.
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2.4.3 System Menu

Press the [MENU] button and the system menu, shown in Figure 2-7 below, will pop up.
Figure 2-7 System menu The system menu contains 8 programs. The programs followed by s have further sub-menus. See Figure 2-8 for the expanded menu.
Figure 2-8 Fully expanded system menu
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Understanding Your Analyzer You can select the desired program as instructed below. If you want to analyze samples select animal type review sample results run the QC program customize system software maintain/service the analyzer calibrate the analyzer shut down the analyzer Select Count Animal Review Quality Control Setup Service Calibration Shutdown
2-13
2.5 Reagents, Controls and Calibrators

Because the analyzer, reagents (diluent, rinse, lyse, probe cleanser and E-Z cleanser), controls, and calibrators are components of a system, performance of the system depends on the combined integrity of all components. You should only use the Mindray-specified reagents (see Appendix B Specifications), which are formulated specifically for the fluidic system of your analyzer in order to provide optimal system performance. If other reagents are used, the analyzer may not meet the performance specified in this manual and may provide unreliable results. All references related to reagents in this manual refer to the reagents specifically formulated for this analyzer. Each reagent package must be examined before use. Inspect the package for signs of leakage or moisture. Product integrity may be compromised in packages that have been damaged. If there is evidence of leakage or improper handling, do not use the reagent.
Store and use the reagents as directed by instructions for use of the reagents. When you have changed the diluent, rinse or lyse, run a background to see if the results meet the requirement. Pay attention to the expiration dates and open-container stability days of all the reagents. Never use expired reagents. After installing new reagents, let the reagents stand for a while before using them.
2.5.1 Diluent
The diluent is formulated to meet the following requirements: To dilute the blood samples; To provide the blood cells with an environment similar to the blood plasma; To maintain the cell volume of each red blood cell and platelet during the count and sizing portion of the measurement cycle; To provide a conductive medium for impedance counting of white and red blood cells and platelets.
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2.5.2 Lyse
The lyse is formulated to meet the following requirements: To rapidly break down red blood cell walls, release the hemoglobin from the cell, and reduce the size of cellular debris to a level that does not interfere with white blood cell counting. To convert hemoglobin to a complex whose absorbance is determined by the hemoglobin concentration.
2.5.3 Rinse
The rinse is formulated to rinse the bath and metering tubes and to provide proper meniscus formation in the metering tubes and maintain it during each measurement cycle.
2.5.4 E-Z Cleanser

The E-Z (enzymatic) cleaner is an enzyme-based isotonic, cleaning solution and wetting agent formulated to clean the fluidic lines and bath.
2.5.5 Probe Cleanser

The probe cleanser is an alkaline cleaning solution formulated to clean the fluidic lines, aperture and bath.
2.5.6 Controls and Calibrators

The controls and calibrators are used to verify accurate operation of and calibrate the analyzer. The controls are commercially prepared whole-blood products used to verify that the analyzer is functioning properly. They are available in low, normal, and high levels. Daily use of all levels verifies the operation of the analyzer and ensures reliable results are obtained. The calibrators are commercially prepared whole-blood products used to calibrate the analyzer. Read and follow the instructions for use to use the controls and calibrators. All references related to controls and calibrators in this manual refer to the controls and calibrators reagents specifically formulated for this analyzer. Controls and calibrators can be purchased from Mindray or Mindray-authorized distributors.
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3
3.1 Introduction
Understanding the System Principles
The two independent measurement methods used in this analyzer are: the impedance method for determining the WBC, RBC, and PLT data; the colorimetric method for determining the HGB. During each analysis cycle, the sample is aspirated, diluted and mixed before the determination for each parameter is performed.
3-1
3.2 Aspiration
This analyzer can process two types of blood samples whole blood samples and prediluted blood samples. If you are going to analyze a whole blood sample, you can simply present the sample to the sample probe and press the aspirate key to aspirate 13L of the sample into the analyzer. If you are going to analyze a capillary blood sample, you should first manually dilute the sample (20 L of capillary sample needs to be diluted by 1.6 mL of diluent) and then present the pre-diluted sample to the sample probe and press the aspirate key to aspirate 0.7 mL of the sample into the analyzer.
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3.3 Dilution
Usually in blood samples, the cells are too close to each other to be identified or counted. For this reason, the diluent is used to separate the cells so that they are drawn through the aperture one at a time as well as to create a conductive environment for cell counting. Moreover, red blood cells usually outnumber white blood cells by 1,000 times. For this reason, lyse needs to be added to the sample to eliminate the red cells before the WBC counting. In addition, to ensure the accuracy of the counting and leukocyte differential, the analyzer sets different RBC dilution ratios and the amount of lyse for different animal type.
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3.4 WBC/HGB Measurement

3.4.1 Volumetric Metering
An accurate cell count cannot be obtained unless the precise volume of diluted sample that passes through the aperture during the count portion of the analysis cycle (the count cycle) is known. This analyzer uses a volumetric metering unit to control the count cycle and to ensure that a precise volume of sample is analyzed. The metering unit controlling the WBC count cycle consists of a metering tube with two optical sensors mounted on it. This tube ensures that a precise amount of diluted sample is measured during each count cycle. The exact amount is determined by the distance between the two optical sensors. The rinse is used to create a meniscus in the metering tube. The count cycle starts when the meniscus reaches the upper sensor and stops when the meniscus reaches the lower sensor. The amount of time required for the meniscus to travel from the upper sensor to the lower sensor is called the WBC Count Time and is measured in seconds. At the end of the count cycle, the measured count time is compared to the pre-defined reference count time (see Chapter 5.3 for details). If the former is less than or greater than the latter by 2 seconds or more, the analyzer will report a WBC bubble or WBC Clog error. Seeing the error message, you can refer to Chapter 11 Troubleshooting Your Analyzer for solutions.
Figure 3-1 Volumetric metering process
3-4
3.4.2 Measurement Principles

WBC measurement WBCs are counted and sized by the impedance method. This method is based on the measurement of changes in electrical resistance produced by a particle, which in this case is a blood cell, suspended in a conductive diluent as it passes through an aperture of known dimensions. An electrode is submerged in the liquid on both sides of the aperture to create an electrical pathway. As each particle passes through the aperture, a transitory change in the resistance between the electrodes is produced. This change produces a measurable electrical pulse. The number of pulses generated indicates the number of particles that passed through the aperture. The amplitude of each pulse is proportional to the volume of each particle. Each pulse is amplified and compared to the internal reference voltage channels, which only accepts the pulses of a certain amplitude. If the pulse generated is above the WBC threshold, it is counted as a WBC.
Figure 3-2 Impedance method of counting and sizing HGB measurement HGB is determined by the colorimetric method. The WBC/HGB dilution is delivered to the WBC bath where it is bubble mixed with a certain amount of lyse, which converts hemoglobin to a hemoglobin complex that is measurable at 525 nm. An LED is mounted on one side of the bath and emits a beam of monochromatic light, whose central wavelength is 525nm, and then is measured by a photo-sensor that is mounted on the opposite side. The signal is then amplified and the voltage is measured and compared to the blank reference reading (readings taken when there is only diluent in the bath). The HGB is calculated per the following equation and expressed in g/L.
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Understanding the System Principles HGB(g/L) = ConstantLog 10 (Blank Photocurrent/Sample Photocurrent)
3.4.3 Derivation of WBC-Related Parameters

WBC WBC (109/ L) is the number of leukocytes measured directly by counting the white blood cells passing through the aperture.
WBC = n 109 / L
Note that NRBCs do not react with the lyse and can be mistaken by the analyzer for white blood cells. If you observe NRBCs in the microscope, correct the system-generated result by the following formula,
WBC'WBC
100 100NRBC
where WBC represents the system-generated white cell number, NRBC the number of NRBCs counted in 100 white cells and WBC the corrected white cell number. WBC differential With the help of the diluent and lyse, this analyzer can size the white cells into three sub-populations - lymphocytes, monocytes and granulocytes. Based on the WBC histogram, this analyzer calculates Lymph, Mon and Gran as follows and express the results in percents.
Lymph% =
PL PL + PM + PG PM
100
Mon% =
PL + PM + PG PG PL + PM + PG
100
Gran% =
100
9
where PL = particles in the lymphocyte region( 10 / L ) PM = particles in the monocyte region( 10 / L ) PG = particles in the granulocyte region( 10 / L ). Having achieved the three parameters above, this analyzer proceeds to calculate the Lymph# , Mon# and Gran# per the following equations and express them in 10 / L . 3-6
9 9 9
Lymph# = Mon# = Gran # =
Lymph% WBC 100
Mon% WBC 100 Gran % WBC 100
WBC histogram Besides the parameters mentioned above, this analyzer also presents a WBC histogram, whose x-coordinate represents the cell volumefLand y-coordinate represents the number of the cells. The histogram is presented in the Analysis Result area of the Count screen when the analysis is done. You can also review the histograms of the stored patient results (see Chapter 7 Reviewing Sample Results). The first three discriminators of the WBC histogram can be adjusted in case you are not satisfied with the result. Note that you cannot adjust them if the WBC result is less than 0.5 or out of the operating range.
3.4.4 HGB
Using the colorimetric method, this analyzer calculates hemoglobin concentration (g/L) as follows. HGB(g/L)=ConstantLog 10 (Blank Photocurrent/Sample Photocurrent)
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3.5 RBC/PLT Measurement

3.5.1 Volumetric Metering
An accurate cell count cannot be obtained unless the precise volume of diluted sample that passes through the aperture during the count cycle is known. This analyzer uses a volumetric metering unit to control the count cycle and to ensure that a precise volume of sample is analyzed for the measurement. The metering unit controlling the RBC/PLT count cycle consists of a metering tube with two optical sensors mounted on it. This tube ensures that a precise amount of diluted sample is measured during each count cycle. The exact amount is determined by the distance between the two optical sensors. The rinse is used to create a meniscus in the metering tube. The count cycle starts when the meniscus reaches the upper sensor and stops when the meniscus reaches the lower sensor. The amount of time required for the meniscus to travel from the upper sensor to the lower sensor is called the RBC Count Time and is measured in seconds. At the end of the count cycle, the measured count time is compared to the pre-defined reference count time (see Chapter 5.3 for details). If the former is less than or greater than the latter by 2 seconds or more, the analyzer will report an RBC bubble or RBC clog error. Seeing the error message, refer to Chapter 11 Troubleshooting Your Analyzer for solutions.
Figure 3-3 Volumetric metering process
3-8
3.5.2 Measurement Principles

RBC/PLT measurement RBCs/PLTs are counted and sized by the impedance method. This method is based on the measurement of changes in electrical resistance produced by a particle, which in this case is a blood cell, suspended in a conductive diluent as it passes through an aperture of known dimensions. An electrode is submerged in the liquid on both sides of the aperture to create an electrical pathway. As each particle passes through the aperture, a transitory change in the resistance between the electrodes is produced. This change produces a measurable electrical pulse. The number of pulses generated indicates the number of particles that passed through the aperture. The amplitude of each pulse is proportional to the volume of each particle. Each pulse is amplified and compared to the internal reference voltage channels, which only accepts the pulses of a certain amplitude. If the pulse generated is above the RBC/PLT lower threshold, it is counted as an RBC/PLT.
Figure 3-4 Impedance method of counting and sizing
3.5.3 Derivation of RBC-Related Parameters

RBC RBC (1012/L) is the number of erythrocytes measured directly by counting the erythrocytes passing through the aperture. MCV Based on the RBC histogram, this analyzer calculates the mean cell volume (MCV) and 3-9
Understanding the System Principles expresses the result in fL. This analyzer calculates the HCT (%), MCH (pg) and MCHC (g/L) as follows:
HCT =
RBC MCV 10 HGB RBC HGB 100 HCT
MCH =
MCHC =
Where the RBC is expressed in 1012/L, MCV in fL and HGB in g/L. RDW Based on the RBC histogram, this analyzer calculates the CV (Coefficient of Variation) of the erythrocyte distribution width. RBC Histogram Besides the parameters mentioned above, this analyzer also presents an RBC histogram, whose x-coordinate represents the cell volumefLand y-coordinate represents the number of the cells. The histogram is presented in the Analysis Result area of the Count screen when the analysis is done. You can also review the histograms of the stored patient results (see Chapter 7 Reviewing Sample Results). The two discriminators of the RBC histogram can be adjusted in case you are not satisfied with the result. Note that you cannot adjust them if the RBC result is less than 0.2 or out of the operating range.
3.5.4 Derivation of PLT-Related Parameters

PLT PLT (109/L) is measured directly by counting the platelets passing through the aperture. MPV Based on the PLT histogram, this analyzer calculates the mean platelet volume (MPV, fL). PDW Platelet distribution width (PDW) is the geometric standard deviation (GSD) of the platelet size distribution. Each PDW result is derived from the platelet histogram data and is reported as 10 (GSD). 3-10
PCT This analyzer calculates the PCT as follows and express it in . Where the PLT is expressed in 109/L and the MPV in fL.
PCT =
PLT MPV 10000
PLT Histogram Besides the parameters mentioned above, this analyzer also presents a PLT histogram, whose x-coordinate represents the cell volumefLand y-coordinate represents the number of the cells. The histogram is presented in the Analysis Result area of the Count screen when the analysis is done. You can also review the histograms of the stored patient results (see Chapter 7 Reviewing Sample Results). The two discriminators of the PLT histogram can be adjusted in case you are not satisfied with the result. Note that you cannot adjust them if the PLT result is less than 10 or out of the operating range.
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3.6 Wash
After each analysis cycle, each element of the analyzer is washed. The sample probe is washed internally and externally with diluent; The bath is washed with diluent and rinse; The metering tube is washed with rinse; The rest of the fluidic system is washed by diluent.
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4
4.1 Introduction
Installing Your Analyzer
This chapter introduces how to install the BC-2800Vet. To ensure all system components function correctly and to verify system performance, Mindray-authorized representatives will handle the installation and initial software setup.
Installation by personnel not authorized or trained by Mindray may damage your analyzer. Do not install your analyzer without the presence of Mindray-authorized personnel.
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4.2 Installation Requirements

Before installation, you should ensure that the following space, power and environmental requirements are met.
4.2.1 Space Requirements

Check the site for proper space allocation. In addition to the space required for the analyzer itself, arrange for at least 28 cm on each side, which is the preferred access to perform service procedures; at least 10 cm behind for cabling and ventilation; enough room on or below the countertop to accommodate the diluent, rinse and waste containers.
4.2.2 Power Requirements

Check the availability of a power outlet that meets the following requirements Voltage: 100 to 240 VAC Frequency: 50/601 Hz Power: 180 VA Fuse: 250V T4A
Make sure the analyzer is properly grounded. Replace fuse only with the type and rating specified. Make sure the electrical outlet meets the requirements.
Before connecting the power cord, make sure the power switch at the back of the analyzer is placed in the off (O) position.
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4.2.3 General Environment

Operating temperature: 15 to 30 . Relative humidity: 30% to 85%. Atmospheric pressure: 60 kPa to 106 kPa. The environment should be as free as possible from dust, mechanical vibrations, loud noises, and electrical interference. Do not place the analyzer near brush-type motors, flickering fluorescent lights, and electrical contacts that regularly open and close. Do not place the analyzer in direct sunlight or in front of a source of heat or drafts.
Do not place the analyzer in a flammable or explosive environment.
Do not place any container on the top of the analyzer.
If the ambient temperature is outside the specified operating range, the analyzer will alarm you for abnormal ambient temperature and the analysis results may be unreliable. See Chapter 11 Troubleshooting Your Analyzer for solutions.
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4.3 Unpacking
4.3.1 Unpacking and Inspecting the Analyzer
Your analyzer is tested before it is shipped from the factory. International symbols and special handling instructions tell the carrier how to treat this electronic instrument. When you receive your analyzer, carefully inspect the carton. If you see any signs of mishandling or damage, contact Mindray customer service department or your local distributor immediately. When you are sure the carton is fine, follow the steps below to unpack the analyzer: Place the carton on the floor upright with the arrows on the side upwards; Remove the tape and take out the accessory box. Check the accessories against the packing list. Notify the Mindray customer service department or your local distributor immediately if you find anything missing; Open the main box and check the items inside against the packing list. Notify the Mindray customer service department or your local distributor immediately if you find anything missing; Remove the top protective foam, carefully carry out the analyzer from the box and place it on the countertop. Remove the foam fixing the sample probe.
Retain the shipping carton and all the packing materials, as they can be used for packaging if analyzer must be reshipped.
4.3.2 How to move the analyzer

If the carton is intact, you may use a plate and fork-lifter to move the analyzer for a short distance. If your analyzer has been used, do the Empty tubing procedure and shut it down before moving it. For a short - distance moving on a smooth ground, you may use a trolley to facilitate the transportation. During the moving process, protect the LCD and the sample probe from excessive force and from contact with other objects. Keep the analyzer upright during the moving process. Do not tilt or incline it. Do your best to minimize the mechanical shock when moving the analyzer. After a long-distance moving, check and tune the analyzer before using it. 4-4
4.4 Installation Procedure
Dispose of reagents, waste, samples, consumables, etc. according to government regulations. The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor.
Use the specified reagents. After installing new reagents, let them stand for a while before using them. Never use expired reagents. To prevent contamination, tighten the container caps when the installation is finished.
Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.
4.4.1 Connecting Regents

On the back of the instrument you will find a fluidic connection equipped with 4 plastic plugs. These plugs are closed by protection caps to prevent dust and the leaking of liquids during transportation. Take off these caps by unscrewing them and keep them in a safe place for future transportation.
Connecting the diluent container

1. Take out the diluent pickup tube (the one with a green connector) from the accessory box. 4-5
2. Remove the container cap and insert the tube end that has no connector into the diluent container and tighten the cap until properly secured, as Figure 4-1 shows;
Diluent Green tubing connector
Figure 4-1 Connecting the diluent container 3. Locate the green fitting marked DILUENT in the lower right corner of the back of the analyzer; 4. Plug the green connector of the tube into the fitting and turn it clockwise until properly secured.
Connecting the rinse container

1. Take out the rinse pickup tube (the one with a blue connector) from the accessory box. 2. Remove the container cap and insert the tube end that has no connector into the rinse container and tighten the cap until properly secured, as Figure 4-2 shows;
Rinse Blue tubing connector
Figure 4-2 Connecting the rinse container 4-6
Installing Your Analyzer 3. Locate the blue fitting marked RINSE in the lower right corner of the back of the analyzer. 4. Plug the blue connector of the tube into the fitting and turn it clockwise until properly secured.
Connecting the lyse container

1. Take out the lyse pickup tube (the one with an orange connector) from the accessory box. 2. Remove the container cap and insert the tube end that has no connector into the container and tighten the cap until properly secured, as Figure 4-3 shows;
Lyse Orange tubing connector
Figure 4-3 Connecting the lyse container 3. Locate the orange fitting marked LYSE in the lower right corner of the back of the analyzer; 4. Plug the orange connector of the tube into the fitting and turn it clockwise until properly secured.
Connect the waste container

1. Take out the waste tube (the one with a red connector) from the accessory box; 2. Locate the red fitting marked WASTE in the lower right corner of the back of the analyzer; 3. Plug the red connector of the tube into the fitting and turn it clockwise until properly secured; 4. Prepare a container to receive the waste and place it on or below the countertop; 5. Insert the waste tube into the waste container.
4.4.2 Installing Recorder Paper
4-7
Remove the protective paper between the recorder head and the roller inside the recorder before installing recorder paper.
Follow the procedure below to install the recorder paper. 1. 2. 3. 4. Use the latch at the upper right corner of the recorder door to pull the door open. Insert a new roll into the compartment as shown below. Close the recorder door. Check if paper is installed correctly and the paper end is feeding from the top.
Paper roll
Figure 4-1 Installing recorder paper
Use only specified recorder paper. Otherwise, it may cause damage to the recorder head, or the recorder may be unable to print, or poor print quality may result. Never pull the recorder paper with force when a recording is in process. Otherwise, it may cause damage to the recorder. Do not leave the recorder door open unless you install paper or remove trouble. Improper installation of recorder paper may jam the paper and/or result in blank printout.
4.4.3 Connecting the Keyboard

Take out the keyboard from the accessory kit and connect it to the keyboard interface marked KB. 4-8
4.4.4 Connecting the Printer (Optional)

Follow the printers instructions for use to connect the printer to the parallel port.
4.4.5 Connecting the Bar-Code Scanner (Optional)

Follow the scanners instructions for use to connect the scanner to the serial port1.
Use the printer and scanner of the specified model.
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4.5 Starting the Analyzer

Take out the power cord from the accessory box. Plug the non-pronged end into the AC input at the back of the analyzer and the pronged end into an electrical outlet. Place the power switch at the back of the analyzer in the ON position (1) to turn on the analyzer. The power indicator light will be illuminated and the screen will display Initializing. The analyzer will sequentially initialize the file, hardware and fluidic systems and the whole initializing process lasts about 4 to 7 minutes. When the initialization is finished, the analyzer will automatically enter the Count screen.
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Customizing the Analyzer Software
5.1 Introduction
The BC-2800Vet is a flexible laboratory instrument that can be tailored to your work environment. You can use the Setup program to customize the software options as introduced in Chapters 5.2 to 5.3.
5-1
5.2 Password
The BC-2800Vet classifies users into two categories: common users (default) and administrators. You need to enter the administrator password to adjust certain options such as Count, Gain, etc.
5.2.1 Entering the administrator password

Press [MENU] to enter the system menu.
Figure 5-1 System menu SELECT Setup Password ( Figure 5-1 ) to enter the Password screen ( Figure 5-2 ).
Figure 5-2 Password screen ENTER 2826 and press [MENU], a message box will pop up to remind you of the current password level, as Figure 5-3 shows, to remind you of the current password level,
5-2
Figure 5-3 A message box to confirm the user level CLICK Yes to confirm the password and exit to the system menu.
5.2.2 Resuming the common user password

Enter the Password screen and the default password is the common user password. Press [MENU] again and a message box will pop up to remind you of the current password level, as Figure 5-4 shows.
Figure 5-4 A message box to confirm the user level CLICK Yes to confirm the password and exit to the system menu.
5-3
5.3 Editing Settings

You can use the Settings menu to edit system settings. Press [MENU] to enter the system menu. SELECT Setup Settings, as Figure 5-5 shows, to enter the Settings screen, as Figure 5-6 shows.
Figure 5-5 System menu
Figure 5-6 Settings screen
This screen can be interpreted as follows: Setting Groups area (on the left) This area displays the visible or changeable setting groups. You can press [F1] to select the desired group. The selected group is preceded by a . Settings area (on the right) You can change the settings of the items displayed in this area. Help area (on the bottom) 5-4
Customizing the Analyzer Software This area displays useful information to help you move to the next step. At this screen, if you want to acquire help information, press [HELP]; if you want to return to the system menu, press [MENU].
5.3.1 Reagent
You can select the Reagent group to change the settings regarding the reagents and the waste, as Figure 5-7 shows.
Figure 5-7 Reagent settings Selecting the Reagent group Press [F1] to select the Reagent group. Setting remaining volumes for reagents You may set the remaining volumes for the diluent, rinse and lyse. When any of the entered volumes is counted down to zero, the system will remind you to install a new container. 1. SELECT Diluent, Rinse or Lyse in the Remains field as needed; 2. ENTER the desired digits. See Table 5-1 for the valid reagent volumes. Table 5-1 Valid reagent volumes Diluent Allowed range Approx 0 to 30.0 L Rinse Approx 0 to 30.0 L Lyse Approx 0 to 999.0 mL
Entering the usable volume of the waste container You may enter the usable volume of the waste container. When the system counts down the entered volume to 0, it will alert you to empty the waste container. Follow the steps given below to set the volume. 5-5
Customizing the Analyzer Software 1. SELECT Waste Container; 2. ENTER the desired digits.
Entering expiration dates of reagents You can specify the expiration dates for the diluent, rinse and lyse. Once any of these reagents is expired, the system will alert you to install a new container. Follow the steps given below to enter the expiration dates. 1. SELECT Exp. Date; 2. ENTER the desired digits; 3. Note that open reagents are stable for 60 days. The entered expiration date should be the open date + 60 days or the expiration date marked on the packaging of the reagent, whichever is earlier.
Exiting the Reagent group When you have finished changing all the reagent settings, you may 1. Press [F1] to select another setting group you want to change; or 2. Press [MENU] and a message box will pop up to remind you to save the changes, as Figure 5-8 shows. CLICK Yes to save the changes and exit to the system menu; or CLICK No to exit to the system menu without saving the changes.
Figure 5-8 Saving changes Note that if any entered value is beyond the valid range, a message box will pop up after you have pressed [MENU]. CLICK Yes to close the message box and clear the invalid values.
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Figure 5-9 A message box to remind you of the erroneous entry
5.3.2 Printing and Communication Settings

You can select the Print & comm. group to change the printing and communication settings to your own need, as Figure 5-10 shows.
Figure 5-10 Printing and communication settings Selecting the Print & comm. group Press [F1] to select the Print & comm. group. Selecting the printing device To select a printing device, SELECT Recorder or Printer from the Device pull-down list, as Figure 5-10 shows. Selecting the printing format To select a printing format, SELECT Vertical or Horizontal from the Format pull-down list,
For the external printer, you can only select Vertical.
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Printout with histogram SELECT With histogram, if you want to include histograms in the printout, as Figure 5-10 shows. To deselect this option, SELECT it again. Printout with WBC differential results The WBC differential count is applicable to cats, dogs, horses, rats, mice, rabbits, monkeys and cows only. You can choose whether to include the differential results in the printout. To include the differential results, SELECT With WBC diff. result. To deselect this option, SELECT it again. Printout with PLT-related results You can choose whether to include the PLT-related results in the printout. To include the PLT-related results, SELECT With PLT-related result. To deselect this option, SELECT it again. Auto printing The auto printing function refers to the analyzers ability to automatically print out the analysis results once they are done. To activate this function, SELECT AutoPrint. To deactivate the function, SELECT it again. Setting baud rate To choose one of the five baud rates19200, 9600, 4800, 2400 and 1200, SELECT the desired baud rate from the Baud pull-down list, as Figure 5-11 shows.
Figure 5-11 Selecting baud rate
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Customizing the Analyzer Software Selecting parity To choose the Odd, Even or None (default) check, SELECT the desired check from the Parity pull-down list, as Figure 5-12 shows.
Figure 5-12 Setting parity Activating/deactivating handshake If the Handshake function is activated, to start the transmission this analyzer will send a handshake signal to an external computer and wait for the response. If the computer does not respond, this analyzer will abort the transmission and give an alarm for the transmission error. If the Handshake function is deactivated, this analyzer will transmit data to the external computer regardless of the response. This function is deactivated by default. To activate or deactivate this option, SELECT Yes or No from the Handshake pull-down list, as Figure 5-13 shows.
Figure 5-13 Setting handshake 5-9
Customizing the Analyzer Software Auto Communication The auto communication function refers to the analyzers ability to automatically transmit the analysis results to an external computer once they are done. To activate or deactivate this function, SELECT On or Off from the AutoComm pull-down list in the Transmit field, as Figure 5-14 shows.
Figure 5-14 Auto communication Editing report titles (external keyboard needed) To edit the title of an analysis report, 1. SELECT Recorder or Printer in the Report Title field, depending on the selected printing device; 2. ENTER the desired report title.
Exiting the Print & comm. group When you have finished changing all the printing and transmission settings, you may press [F1] to select another setting group you want to change; or press [MENU] and a message box will pop up to remind you to save the changes, as Figure 5-15 shows. CLICK Yes to save the changes and exit to the system menu; or CLICK No to exit to the system menu without saving the changes.
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Figure 5-15 Saving changes
5.3.3 Date and Time

You can select the Date & time group to set the system date and time, as Figure 5-16 shows. Follow the instructions given below to do so.
Figure 5-16 Setting date and time
Selecting the Date & time group Press [F1] to select the Date & time group. Setting the system date 1. SELECT Year, Month or Day; 5-11
Customizing the Analyzer Software 2. ENTER the desired digits.
Selecting the date format You may choose one of the three formats YYYY-MM-DD, MM-DD-YYYY and DD-MM-YYYY. To do so, SELECT the desired format from the Format pull-down list, as Figure 5-17 shows.
Figure 5-17 Setting date format
Setting the system time 1. SELECT Hour, Minute or Second; 2. ENTER the desired digits.
Exiting the Date & time group When you have finished changing all the date and time settings, you may 1. Press [F1] to select another setting group you want to change; or; 2. Press [MENU] and a message box will pop up to remind you to save the changes, as Figure 5-18 shows. CLICK Yes to save the changes and exit to the system menu; or CLICK No to exit to the system menu without saving the changes.
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5.3.4 Gain
You can select the Gain group to view or change (if you have the administrator password) the WBC, RBC and HGB gains.
Figure 5-19 Setting gain Selecting the Gain group Press [F1] to select the Gain group, as Figure 5-19 shows. Setting the RBC gain When the difference between the actual MCV result and the expected result exceeds 6%,
5-13
Customizing the Analyzer Software you need to change the RBC gain. For example, assuming the expected MCV result is 90.0fL, while the actual analysis result is 82.0fL, then
ExpectedMCV 90.0 100% 100% = 109.8% ActualMCV 82.0

You should adjust the RBC gain to 109.8% as close as possible. Follow the steps given below to do so. 1. Enter the administrator password as introduced in Chapter 5.2.1; 2. At the Settings screen, press [F1] to select the Gain group; 3. SELECT RBC, as Figure 5-20 shows; 4. ENTER the desired gain so that the adjustment becomes as close to 109.8% as possible.
Figure 5-20 Setting RBC gain Setting the HGB gain You may adjust the HGB gain to change the HGB blank voltage, which usually should be set between 3.4 to 4.8V (4.5V recommended). Follow the steps given below to set the HGB gain. 1. Enter the administrator password as introduced in Chapter 5.2.1; 2. At the Settings screen, press [F1] to select the Gain group; 3. SELECT HGB; 4. ENTER the desired gain so that the HGB blank voltage is between 3.4 to 4.8V, as Figure 5-21 shows.
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Figure 5-21 Setting HGB gain Exiting Gain group When you have finished changing all the gain settings, you may 1. Press [F1] to select another setting group you want to change; or; 2. Press [MENU] and a message box will pop up to remind you to save the changes, as Figure 5-22 shows. CLICK Yes to save the changes and exit to the system menu; or CLICK No to exit to the system menu without saving the changes.
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5.3.5 Count
You can select the Count group to view or change (if you have the administrator password) the parameter units and count time.
Figure 5-23 Setting unit and count time Selecting the Count group Press [F1] to select the Count group, as Figure 5-23 shows. Selecting parameter units This analyzer provides multiple units for certain parameters. Refer to Table 5-2 for all the selectable units of all parameters. The 19 parameters are divided into 11 groups based on their units and you can only select unit for the first parameter of a group. Pay special attention to the HGB group, which includes HGB, MCHC and MCH. When you select g/L or g/dL as the unit of HGB, the default unit for MCH is pg; when you select mmol/L as the unit of HGB, the default unit of MCH is fmol. Table 5-2 Units of Parameters Parameter WBC Lymph# Mon# Gran# Lymph% Mon% Gran% ***.* ***.* **** ***.* **.* .*** % Display format Unit 109/L 103/uL 102/uL /nL Remarks Default / / / Default
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Customizing the Analyzer Software HGB, MCHC *** **.* **.* RBC **.** **.** **** **.** HCT **.* .*** MCV ***.* ***.* RDW PLT **.* **** **** ***.* **** MPV ***.* ***.* PDW PCT **.* .*** *.** MCH *.*** **.** g/L g/dL mmol/L 1012/L 106/uL 104/uL /pL % L/L fL um3 % 109 /L 103 /uL 104 /uL /nL fL um3 / % mL/L pg fmol Default / / Default / / / Default / Default / Default Default / / / Default / Default Default / Default /
Follow the steps given below to select the units. 1. Enter the administrator password as introduced in Chapter 5.2.1; 2. At the Settings screen, press [F1] to select the Count group; 3. SELECT the desired unit from the pull-down list of the desired parameter, as Figure 5-24 shows.
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Figure 5-24 Selecting parameter unit Setting count time If the WBC or RBC count time is inappropriately set, the system may give false alarms for clogs or bubbles. When this happens, follow the steps given below to change the WBC or RBC count time. Refer to the actual count time (see Chapter 10.4.1 for details) when editing the count time. Follow the steps given below to set the count time. 1. Enter the administrator password as introduced in Chapter 5.2.1; 2. At the Settings screen, press [F1] to select the Count group; 3. SELECT WBC or RBC, as Figure 5-25 shows; 4. ENTER the desired digits.
Figure 5-25 Setting count time 5-18
Customizing the Analyzer Software Exiting the Count group When you have finished changing all the parameter units and count time settings you want to change, you may 1. Press [F1] to select another setting group you want to change; or; 2. Press [MENU] and a message box will pop up to remind you to save the changes, as Figure 5-26 shows. CLICK Yes to save the changes and exit to the system menu; or CLICK No to exit to the system menu without saving the changes.
5.3.6 Reference Range

If you have the administrator password, you can set a reference range for every parameter of the currently selected animal (see Chapter 6.5 for how to select the animal). The system will flag any parameter result that exceeds this range with either an H (higher than the upper limit) or L (lower than the lower limit). The default ranges are given. Note that the default ranges are for references only. Each laboratory is encouraged to establish its own references. As for the user-defined animals, reference ranges are available for 12 parameters, excluding the six parameters related to the WBC differential count. Setting reference ranges (administrators only) 1. Enter the administrator password as introduced in Chapter 5.2.1; 2. At the Settings screen, press [F1] to select the Ref. Range group, as Figure 5-27 shows;
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Figure 5-27 Setting reference range 3. SELECT the desired parameter and ENTER the desired digits for the upper and lower limits; 4. Press [F2] to save the changes. If the changes are successfully saved, a message box shown in Figure 5-28 will pop up; CLICK the Yes to close the message box;
Figure 5-28 A message box to note data saved 5. If some entered limits are invalid, a message box shown in Figure 5-29 will pop up. CLICK Yes and reenter valid number;
Figure 5-29 A message box to note an erroneous entry 6. If you want to resume the default settings, press [F4]. Exiting the Ref. Range group When you have finished changing all the reference range settings you want to change, you may 1. Press [F1] to select another setting group you want to change; or; 2. Press [MENU] to exit to the system menu.
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5.3.7 Other Settings

You can select the Other group to view or change other settings. Selecting the Other group Press [F1] to select the Other group. Muting beeper This analyzer beeps when an error occurs. You can mute the beeper by pressing any key or leave it beeping until the errors are removed. If you prefer the former, SELECT Enabled from the Any key to mute pull-down list ; if you prefer the latter, SELECT Disabled from the Any key to mute pull-down list. See Figure 5-30 shows.
Figure 5-30 Selecting how to mute the beeper Selecting LCD contrast Follow the steps given below to adjust the LCD contrast. 1. SELECT LCD contrast, as Figure 5-31 shows; 2. ENTER the desired contrast (0 to 255).
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Figure 5-31 Select LCD contrast
Setting alarm time Follow the steps given below to set for how long (2s to120s) the error messages listed in Table 5-3 should be displayed on the screen. Table 5-3 Error List No. 1 4 7 10 Error Communication Error Environmental Temperature Abnormal HGB Adjustment RBC Clog 8 11 WBC Clog RBC Bubbles 9 WBC Bubbles No. 2 5 Error Scanner Error Background Abnormal No. 3 6 Error Scanner Communication Error HGB Error
1. SELECT Alarm time(s), as Figure 5-32 shows; 2. ENTER the desired digits.
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Figure 5-32 Setting alarm time Selecting PMB color (administrator password needed) The PMB color refers to the background color of the screen when your analyzer is in the prediluted mode. Follow the steps below to select the PMB color. 1. Enter the administrator password as introduced in Chapter 5.2.1; 2. At the Settings screen, press [F1] to select the Other group; 3. SELECT Black (default) or Blue from the PMB color pull-down list, as Figure 5-33 shows.
Figure 5-33 Setting PMB color
Exiting the Other group
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Customizing the Analyzer Software When you have finished changing all the Other settings you want to change, you may 1. Press [F1] to select another setting group you want to change; or; 2. Press [MENU] and a message box will pop up to remind you to save the changes, as Figure 5-34 shows. SELECT Yes to save the changes and exit to the system menu; or SELECT No to exit to the system menu without saving the changes.
5.3.8 Special Fluidic Settings (for user-defined animals)

For the user-defined animals, the Fluidic settings group will be enabled. You can select the Fluidic settings group to view or change (if you have the administrator password) the listed fluidic settings of the currently selected animal. Note that the default settings are for your reference only. You need to find the settings that fit the animal the best.
Figure 5-35 Fluidic settings screen 5-24
Adjusting the fluidic settings 1. Enter the administrator password as introduced in Chapter 5.2.1; 2. At the Settings screen, press [F1] to select the Fluidic settings group;
3. SELECT the desired settings; 4. ENTER the desired digits for Lyse used (wb) or Lyse used (pre) (Figure 5-35),
Exiting the Fluidic settings group When you have finished changing all the parameter units and count time settings you want to change, you may 1. Press [F1] to select another setting group you want to change; or; 2. Press [MENU] and a message box will pop up to remind you to save the changes, as Figure 5-36 shows. CLICK Yes to save the changes and exit to the system menu; or CLICK No to exit to the system menu without saving the changes.
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6
6.1 Introduction
Operating Your Analyzer
This chapter provides step-by-step procedures for operating your analyzer on a daily basis.
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6.2 Initial Checks

Perform the following checks before turning on the analyzer. 1. Check and make sure the waste container is empty; 2. Check and make sure there are enough reagents;
Dispose of reagents, waste, samples, consumables, etc. according to government regulations.
3. Checking tubing and power connections; Check and make sure the diluent, rinse and waste tubes are properly connected and not bent; Check and make sure the power cord of the analyzer is properly plugged into an electrical outlet.
4. Checking the printer (optional) and recorder; Check and make sure enough printer or recorder paper is installed. Check and make sure the power cord of the printer is properly plugged into an electrical outlet. Check and make sure the printer cable is properly connected to the analyzer. 5. Check keyboard connection. Check and make sure the keyboard is properly connected to the keyboard interface (marked KB) of the analyzer.
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6.3 Power-on
Place the power switch at the back of the analyzer in the ON position (1) to turn on the analyzer. The power indicator light will be illuminated and the screen will display Initializing. The analyzer will sequentially initialize the file, hardware and fluidic systems and the whole initializing process lasts 3 to 5 minutes, depending on how the analyzer was previously shut down. If any error occurs during the initialization, the analyzer will display the error messages in the lower left corner of the screen. You should remove all the errors before running any sample. See Chapter 11 Troubleshooting Your Analyzer for solutions.
Running samples with the abnormal background error present will lead to misleading results.
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6.4 Daily Quality Control

Before running any samples, run the controls. See Chapter 8 Using the QC Programs for details.
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6.5 Selecting Animal

1. Press [MENU] to enter the system menu, as Figure 6-1 shows;
Figure 6-1 System menu 2. SELECT Animal to enter the Animal screen, as Figure 6-2 shows;
Figure 6-2 Animal screen 3. SELECT the desired animal; 4. Press [MENU] to exit to the system menu.
Once you have selected another animal, the analysis result and sample information regarding the current sample will be cleared, but the next sample ID will remain unchanged.
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6.6 Define an animal

You can define a maximum of 3 user-defined animals. Follow the steps below to do so: 1. At the Animal screen, press the arrow keys as needed to move the cursor to a user-defined animal (Animal1 to Animal3); 2. Press [F1] to activate the edit box and ENTER the animal name; 3. Press [F1] to deactivate the edit box.
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6.7 Sample Collection and Handling
Avoid direct contact with blood samples.
Do not re-use disposable products.
6.7.1 Whole Blood Samples

Collect and handle the whole blood sample as follows: 1. Collect venous blood; 2. Rapidly and thoroughly mix the blood with the anticoagulant, K2EDTA (1.5 to 2.2mg/mL ).
For the whole blood samples to be used for WBC differential or PLT count, you shall store them at the room temperature and run them within 4 hours after collection. If you do not need the PLT, MCV and WBC differential results, you can store the samples in a refrigerator (2 to 8) for 24 hours. You need to warm the refrigerated samples at room temperature for at least 30 minutes before running them. Mix any sample that has been prepared for a while before running it.
6.7.2 Prediluted Samples

Collect and handle the prediluted sample as follows: 1. Press [MENU] and SELECT Count to enter the Count screen; 6-7
Operating Your Analyzer 2. Press [MODE] to select analysis mode PB; 3. Press [DILUENT] and a message box will pop up to instruct you how to dispense the diluent into the sample tube, as Figure 6-3 shows;
Figure 6-3 A message box showing you how to dispense diluent 4. Present a clean sample tube to the sample probe and make sure the tube is tilted towards the probe, as Figure 6-4 shows, to avoid spills and bubbles. Press the aspirate key to dispense 0.7mL of diluent (the dispensing volume is controlled by the analyzer) into the tube;
Figure 6-4 How to dispense diluent 5. When the dispensing is finished, press [ENTER] to close the message box; 6. Add 20L of capillary blood to the diluent and shake the tube to mix the sample.
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Keep dust from the prepared diluent. After mixing the capillary sample with the diluent, wait 3 minutes before running the sample. Run the prediluted samples within 15 minutes after the mixing. Mix any sample that has been prepared for a while before running it. Evaluate predilute stability based on your laboratorys sample population and sample collection techniques or methods.
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6.8 Running Whole Blood Samples

Press [MENU] and SELECT Count to enter the Count screen, as Figure 6-5 shows.
Figure 6-5 Count screen Press [MODE] to select WB mode;
Select proper reference range as instructed in Chapter 5.3.6 before running the samples. Otherwise, the obtained results may be erroneously flagged.
6.8.1 Entering Sample Information

At the Count screen, you can enter the sample information for the next sample (the sample to be analyzed) and edit the sample information for the current sample (the sample whose analysis results are displayed on the screen).
Entering the sample inforamtion of the next sample(external keyboard needed)

To enter the sample ID of the next sample, you may At the Count screen, use the bar-code scanner (if available) to scan the sample ID into the analyzer; or At the Count screen, press [F1] to enter the Enter sample information window and ENTER the information of the next sample, as Figure 6-6 shows.
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Figure 6-6 Entering ID of the next sample Entering the sample ID Enter the sample ID into the ID box. Selecting animal gender SELECT the desired item from the Gender pull-down list, as Figure 6-7 shows. Note that you can select blank in case you are not aware of the patient gender.
Figure 6-7 Selecting animal gender of the nest sample Entering the owners name ENTER the owners name into the Owner box. Entering the animals name ENTER the animals name into the Animal box. 6-11
Entering the animals age This analyzer provides three ways for you to enter the patient age in years, in months and in days. To enter the animals age in years: ENTER the desired number, an integer from 0 to 200, into the Years box. To enter the patient age in months: ENTER the desired number, an integer from 0 to 12, into the Months box. To enter the patient age in days: ENTER the desired number, an integer from 0 to 31, into the Days box. Exit edit When you have finished entering all the desired sample information, CLICK the Yes button to save the changes and return to the Count screen. If you do not want to save the entered information, CLICK the No button to return to the Count screen without saving the changes.
Figure 6-8 Dialog box
If you intend to do the background check instead of a patient sample, enter 0 into the ID box. After entering the information into any field of the Sample information box, you can press [enter] to directly finish entering while saving the changes and closing the box.
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Editing sample information of the current sample (external keyboard needed)

Entering Edit At the Count screen, after running an analysis, press [F4] and an edit window will pop up, as Figure 6-9 shows.
Figure 6-9 Entering sample information Selecting animal gender SELECT the desired item from the Gender pull-down list, as Figure 6-10 shows. Note that you can select blank in case you are not aware of the patient gender.
Figure 6-10 How to select the patient gender Entering the owners name ENTER the owners name into the Owner box. Entering the animals name ENTER the animals name into the Animal box. Entering the animal age This analyzer provides three ways for you to enter the patient age in years, in months and in 6-13
Operating Your Analyzer days. To enter the animals age in years: ENTER the desired number, an integer from 0 to 200, into the Years box. To enter the patient age in months: ENTER the desired number, an integer from 0 to 12, into the Months box. To enter the patient age in days: ENTER the desired number, an integer from 0 to 31, into the Days box. Exit edit When you have finished entering all the desired sample information, CLICK the Yes button to save the changes and return to the Count screen. If you do not want to save the entered information, CLICK the No button to return to the Count screen without saving the changes.
After entering the information into any field of the Sample information box, you can press [enter] to directly finish entering while saving the changes and closing the box.
6.8.2 Running the Samples
The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it. Do not re-use such disposable product as collection tubes, test tubes, capillary tubes, etc.
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Keep the sample probe tip away from the tube bottom; otherwise the aspiration volume may be inaccurate. When the aspiration is done, remove the sample tube only when the sample probe is out of the tube.
1. At the Count screen, be sure the System Status area displays Ready and the Count Mode area displays WB; 2. Present the mixed sample to the sample probe so that the tip is well into the tube, and press the aspirate key. The System Status area will display Running and the analyzer will start aspirating sample; 3. When you hear the beep and the sample probe is out of the tube, remove the sample tube. The sample probe will retract into the analyzer and the analysis progress will be displayed on the screen; 4. When the analysis is finished, the result will be displayed on the screen and the sample ID will automatically increase by 1 and the sample probe will be repositioned. And if the auto print function is enabled, the analysis result will be automatically printed out; 5. Repeat the above steps on other samples.
If the analyzer detects WBC/RBC clogging or bubbles during the analysis, the corresponding error messages will be displayed in the error message area and the results of all the related parameters will be invalidated. See Chapter 11 Troubleshooting Your Analyzer for solutions. If the ambient temperature is outside the specified operating range, the analyzer will alarm you for abnormal ambient temperature and the analysis results may be unreliable. See Chapter 11 Troubleshooting Your Analyzer for solutions.
6.8.3 Special Functions

Automatic saving of analysis results
This analyzer automatically saves a maximum of 10,000 sample results. When the maximum number has been reached, the newest result will overwrite the oldest.
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Parameter flags
If the analysis result is followed by an H or L, it means the analysis result has exceeded the upper or lower limit of the reference range. If you see *** as opposed to the result, it means the result is either unreliable or out of the operating range. If the WBC result is less than 0.5 109/L, this analyzer will not perform the differential analysis and all the related parameter values will be non-numeric (***).
The result of the background check will not be flagged.
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6.9 Running Prediluted Samples

Press [MENU] and SELECT Count to enter the Count screen, as Figure 6-11 shows.
Figure 6-11 Count screen Press [MODE] to select PB mode;
Select proper reference range as instructed in Chapter 5.3.6 before running the samples. Otherwise, the obtained results may be erroneously flagged.
6.9.1 Entering Sample Information

At the Count screen, you can enter the sample information for the next sample (the sample to be analyzed) and edit the sample information for the current sample (the sample whose analysis results are displayed on the screen).
Entering the sample information of the next sample(external keyboard needed)

To enter the sample ID of the next sample, you may At the Count screen, use the bar-code scanner (if available) to scan the sample ID into the analyzer; or At the Count screen, press [F1] to enter the Enter sample information window and ENTER the information for the next sample, as Figure 6-12 shows. 6-17
Figure 6-12 Entering ID of the next sample Entering the sample ID Enter the sample ID into the ID box. Selecting animal gender SELECT the desired item from the Gender pull-down list, as Figure 6-13 shows. Note that you can select blank in case you are not aware of the patient gender.
Figure 6-13 Selecting animal gender of the nest sample Entering the owners name ENTER the owners name into the Owner box.
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Operating Your Analyzer Entering the animals name ENTER the animals name into the Animal box. Entering the animals age This analyzer provides three ways for you to enter the patient age in years, in months and in days. To enter the animals age in years: ENTER the desired number, an integer from 0 to 200, into the Years box. To enter the patient age in months: ENTER the desired number, an integer from 0 to 12, into the Months box. To enter the patient age in days: ENTER the desired number, an integer from 0 to 31, into the Days box. Exit edit When you have finished entering all the desired sample information, CLICK the Yes button to save the changes and return to the Count screen. If you do not want to save the entered information, CLICK the No button to return to the Count screen without saving the changes.
Figure 6-14 Dialog box
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If you intend to do the background check instead of a patient sample, enter 0 into the ID box. After entering the information into any field of the Sample information box, you can press [enter] to directly finish entering while saving the changes and closing the box.
Editing sample information of the current sample (external keyboard needed)

Entering Edit At the Count screen, after running an analysis, press [F4] and an edit window will pop up, as Figure 6-15 shows.
Figure 6-15 Entering sample information Selecting animal gender SELECT the desired item from the Gender pull-down list, as Figure 6-16 shows. Note that you can select blank in case you are not aware of the patient gender.
Figure 6-16 How to select the patient gender
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Operating Your Analyzer Entering the owners name ENTER the owners name into the Owner box. Entering the animals name ENTER the animals name into the Animal box. Entering the animals age This analyzer provides three ways for you to enter the patient age in years, in months and in days. To enter the animals age in years: ENTER the desired number, an integer from 0 to 200, into the Years box. To enter the patient age in months: ENTER the desired number, an integer from 0 to 12, into the Months box. To enter the patient age in days: ENTER the desired number, an integer from 0 to 31, into the Days box. Exit edit When you have finished entering all the desired sample information, CLICK the Yes button to save the changes and return to the Count screen. If you do not want to save the entered information, CLICK the No button to return to the Count screen without saving the changes.
After entering the information into any field of the Sample information box, you can press [enter] to directly finish entering while saving the changes and closing the box.
6.9.2 Running the Samples
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1. At the Count screen, be sure the System Status area displays Ready and the Count Mode area displays PB; 2. Present the mixed sample to the sample probe so that the tip is well into the tube, and press the aspirate key. The System Status area will display Running and the analyzer will start aspirating sample; 3. When you hear the beep and the sample probe is out of the tube, remove the sample tube. The sample probe will retract into the analyzer and the analysis progress will be displayed on the screen; 4. When the analysis is finished, the result will be displayed on the screen and the sample ID will automatically increase by 1 and the sample probe will be repositioned. And if the auto print function is enabled, the analysis result will be automatically printed out; 5. Repeat the above steps on other samples.
6.9.3 Special Functions

Automatic saving of analysis results
This analyzer automatically saves a maximum of 10,000 sample results. When the maximum number has been reached, the newest result will overwrite the oldest.
Parameter flags
If the analysis result is followed by an H or L, it means the analysis result has exceeded the upper or lower limit of the reference range. If you see *** as opposed to the result, it means the result is either unreliable or out of the operating range. 6-22
Operating Your Analyzer If the WBC result is less than 0.5 109/L, this analyzer will not perform the differential analysis and all the related parameter values will be non-numeric (***).
The result of the background check will not be flagged.
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6.10 Shutdown
Perform the Shutdown procedure to shut down the analyzer daily.
To ensure stable analyzer performance and accurate analysis results, perform the Shutdown procedure to shut down the analyzer after it has been running continuously for 24 hours. Shut down the analyzer strictly as instructed below.
1. Press [MENU] to enter the system menu and SELECT Shutdown, as Figure 6-17 shows;
Figure 6-17 Selecting the shutdown program 2. A message box will pop up to ask you to confirm the shutdown, as Figure 6-18 shows;
Figure 6-18 Shutdown message box 3. CLICK Yes and a window will pop up to instruct you how to shut down the analyzer, as Figure 6-19 shows;
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Figure 6-19 Shutdown window (1)
The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it. The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.
4. Present the E-Z cleanser to the sample probe and press the aspirate key. The analyzer will aspirate the E-Z cleanser and automatically clean the fluidic lines and the bath. The cleaning progress will be displayed on the screen, as Figure 6-20 shows;
Figure 6-20 Shutdown progress bar 5. When the cleaning is finished, a window will pop up to instruct you what to do next, as Figure 6-21 shows.
Figure 6-21 shutdown window (2) 6-25
Operating Your Analyzer 6. Present the E-Z cleanser to the sample probe and press the aspirate key. The analyzer will aspirate the E-Z cleanser and automatically clean the fluidic lines and the bath. The cleaning progress will be displayed on the screen, as Figure 6-22 shows;
Figure 6-22 Shutdown progress bar 7. When the cleaning is finished, place the switch at the back of the analyzer to OFF (O) to turn off the analyzer; 8. Empty the waste container.
Dispose of reagents, waste, samples, consumables, etc. according to government regulations.
Fur and skin debris may block the aperture. Keep the sample clean before using the analyzer to analyze it. Samples of the dogs infected with Filaria Larva in Blood may clog the aperture.
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7
7.1 Introduction
Reviewing Sample Results
The analyzer automatically saves analysis results. Totally 10,000 results can be saved. You can either browse all the saved sample results in general or search for the results of a particular sample or samples.
7-1
7.2 Browsing All Sample Results

To browse all the saved sample results, you can choose either of the following modes: The Histogram mode. In this mode, you can review both parameter values and histograms of the saved sample results, one sample result per screen. The Table mode. In this mode, the sample results are presented in a columnar fashion without histograms (namely you can only see the parameter values). One screen displays a maximum of 8 sample results.
7.2.1 Browsing in the Histogram Mode

Entering the Histogram mode
Press [MENU] to enter the system menu, and SELECT Review ( Figure 7-1 ) to enter the Review screen ( Figure 7-2 ).
Figure 7-2 Sample histogram review screen 7-2
Browsing sample results

Press [] or [] to browse the preceding or following sample result; press [PgUp] or [PgDn] to jump 8 locations (e.g. from location 1 to location 9).
Switching to the Table mode

To switch to the Table mode, press[]; to switch back to the Histogram mode, press[]again.
Jumping to a sample result with known location

Press [F1] and a Goto window will pop up, as Figure 7-3 shows.
Figure 7-3 Goto window ENTER the location into the Location box and press [ENTER] to jump to the desired sample result.
Editing sample information

Press [F2] to edit the sample information, Figure 7-4 shows.
Figure 7-4 Editing sample information ID You cannot edit the sample ID of an analyzed sample. Selecting the animal gender SELECT the desired item from the Gender pull-down list. Note that you can select blank in case you are not aware of the animal gender.
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Entering the owners name ENTER the owners name into the Owner box. Entering the animal name ENTER the animal name into the Animal box. Entering the animal age This analyzer provides three ways for you to enter the animal age in years, in months and in days. The first way is designed for the animals no younger than one year; the second for the animals older than one month and younger than one year; the third for the animals younger than one month. You can choose only one of the three ways to enter the animal age. To enter the patient age in years: ENTER the desired number, an integer from 0 to 200, into the Years box. To enter the patient age in months: ENTER the desired number, an integer from 0 to 12, into the Months box. To enter the patient age in days: ENTER the desired number, an integer from 0 to 31, into the Days box. Yes button When you have finished entering all the desired sample information, CLICK the Yes button to save the changes and return to exit the edit window. No button If you do not want to save the entered information, CLICK the Cancel button to return to exit the edit window.
Printing sample results

Press [PRINT] to print out the current sample result.
7.2.2 Browsing in the Table mode

At the Histogram mode, press[]to switch to the Table mode, as Figure 7-5 shows.
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Figure 7-5 Sample table review screen The sample results are sequentially displayed on the screen, The Loc/Total displayed in the lower right corner of the screen indicates the location of the current sample result (the one whose ID is backlit) and the total number of the sample results.

Press [] or [] to browse the preceding or following sample result; press [PgUp] or [PgDn] to browse the preceding or following screen.
Switching to the Histogram mode

If you are interested in reviewing the histograms of the current sample result, press[]to switch to the Histogram mode. To switch back to the Table mode, press [] again.

You can select certain specific samples for transmission or printing.

Selecting/deselecting a sample result 7-5
Reviewing Sample Results Press [] or [] to move the cursor to the specific sample result and press [ENTER] to select it. The selected sample result will be marked with a *, as sample 118 in Figure 7-7 shows.
Figure 7-7 Selecting a sample result Press [ENTER] again to deselect the sample result. Once the sample is deselected, the * will disappear, as Figure 7-8 shows.
Figure 7-8 Deselecting a sample result Selecting/deselecting multiple sample results Example1: To select the sample results of locations 1 to 5 (sample IDs: 114 to 118), follow the procedure below to do so: 1. Press [F2] to enter the Select window, as Figure 7-9 shows;
7-6
Figure 7-9 Entering the Select window 2. ENTER the start position (00001) into the From box; 3. ENTER the end position (00005) into the To box; 4. CLICK Select and the lower left corner of the Select window will display Select samples, as Figure 7-10 shows;
Figure 7-10 Selecting sample results of locations 1-5 5. CLICK Exit to return to the sample table review screen. The selected sample results will be marked with *, as Figure 7-11 shows.
Figure 7-11 Reviewing the selected results Example2: To deselect the sample results of locations 1 to 5, follow the procedure below to do so: 1. Enter the start and end positions as instructed in steps 1 to 3 of Example1; 2. CLICK De-select and the lower left corner of the Select window will display 7-7
Reviewing Sample Results De-select the results, as Figure 7-12 shows;
Figure 7-12 Deselecting the sample results of locations 1 to 5 3. CLICK Exit to return to the sample table review screen. The * above those sample results will disappear, as Figure 7-13 shows.
Figure 7-13 Reviewing the deselected results Example3: To select the sample results of locations 1 to 5 and 7 to 8, follow the procedure below to do so: 1. Select the sample results of locations 1 to 5 as instructed in steps 1 to 4 of Example1; 2. Select the sample results of locations 7 to 8 as instructed in steps 1 to 4 of Example1; 3. CLICK Exit to return to the sample table review screen. The selected sample results will be marked with *, as Figure 7-14 shows.
7-8
Figure 7-14 Reviewing the selected results Example4:To deselect the sample results of locations 1 to 5 and 7 to 8, follow the procedure below to do so: 1. Deselect the sample results of locations 1 to 5 as instructed in steps 1 to 3 of Example2; 2. Deselect the sample results of locations 7 to 8 as instructed in steps 1 to 3 of Example2; 3. CLICK Quit to return to the sample table review screen. The * above those sample results will disappear, as Figure 7-15 shows.
Figure 7-15 Reviewing the deselected results
Transmitting sample results to a host

You can transmit the selected or all sample results to an external computer (a host). Press [F3] to enter the Transmit window, as Figure 7-16 shows.
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Figure 7-16 Transmit window To transmit the selected sample results to a host, CLICK Selected; To transmit all the sample results, CLICK All; To stop a transmission, CLICK Stop; To return to the sample table review screen, CLICK Exit.

Select the sample results you want to print and press [PRINT]. A message box will pop up to ask you to confirm the printing, as Figure 7-17 shows. CLICK Yes to print out all the selected results; CLICK No to abort the printing.
Figure 7-17 Print message box
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7.3 Searching for Specific Sample Results

7.3.1 Starting a search
At the sample table review screen, press [F4] to enter the Search window, as Figure 7-18 shows.
Figure 7-18 Search window To include a search condition, press [] or [] to move the cursor to the desired condition and press [ENTER] to select the condition, as Figure 7-19 shows.
Figure 7-19 All search conditions are included Selecting the animal type SELCT the desired animal type from the Type pull-down list. Entering the animal name ENTER the animal name into the Animal box. Entering the owners name ENTER the owners name into the Owner box.
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Reviewing Sample Results Entering sample ID ENTER the ID number into the ID box. Selecting the animal gender SELECT the desired item from the Gender pull-down list. Note that you can select blank in case you are not aware of the animal gender. Entering the start and end date ENTER the start date into the Start box; ENTER the end date into the End box. CLICK Yes to start the search. The analyzer will search the saved sample results for matches and report the conclusion at Search Result window, as Figure 7-20 shows. CLICK Yes of the Search Result window to return to the searched sample review screen. The matches found are saved in a database called Searched and you can review them in either the Table mode or the Histogram mode.
Figure 7-20 Search Result window
7.3.2 Reviewing Search Result in the Table Mode
For every search, the analyzer can display a maximum of 500 matches. The matches will be deleted if you have run another sample (including background check), or deleted a sample result, or restarted the analyzer after the search.
Entering the Table mode

At the Review screen, press [] to select the Searched database and then press [] to enter the Table screen, as Figure 7-21 shows.
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Figure 7-21 Table screen The sample results are sequentially displayed on the screen, The Loc/Total displayed in the lower right corner of the screen indicates the location of the current sample result (the one whose ID is backlit) and the total number of the sample results matching the search conditions.

Press [] or [] to browse the preceding or following sample result; press [PgUp] or [PgDn] to browse the preceding or following screen.
Switching to the Histogram mode

If you are interested in reviewing the histograms of the current sample result, press [] to switch to the Histogram mode. To switch back to the Table mode, press[] again.

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You can select certain specific samples for transmission or printing.

Selecting/deselecting a sample result Press [] or [] to move the cursor to the specific sample result and press [ENTER] to select it. The selected sample result will be marked with a *, as sample 118 in Figure 7-23 shows.
Figure 7-23 Selecting a sample result Press [ENTER] again to deselect the sample result. Once the sample is deselected, the * will disappear, as Figure 7-24 shows.
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Figure 7-24 De-selecting a sample result Selecting/deselecting multiple sample results Example1: To select the sample results of locations 1 to 5 (sample IDs:114 to118 ), follow the procedure below to do so: 1. Press [F2] to enter the Select window, as Figure 7-25 shows;
Figure 7-25 Entering the Select window 2. ENTER the start position (00001) into the From box; 3. ENTER the end position (00005) into the To box; 4. CLICK Select and the lower left corner of the Select window will display Select samples, as Figure 7-26 shows;
Figure 7-26 Selecting sample results of locations 1 to 5 7-15
Reviewing Sample Results 5. CLICK Exit to return to the sample table review screen. The selected sample results will be marked with *, as Figure 7-27 shows.
Figure 7-27 Reviewing the selected results Example2: To deselect the sample results of locations 1 to 5, follow the procedure below to do so: 1. Enter the start and end positions as instructed in steps 1 to 3 of Example1; 2. CLICK De-select and the lower left corner of the Select window will display De-select the result, as Figure 7-28 shows;
Figure 7-28 Deselecting the sample results of locations 1 to 5 3. CLICK Exit to return to the sample table review screen. The * above those sample results will disappear, as Figure 7-29 shows.
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Figure 7-29 Reviewing the deselected results Example3: To select the sample results of locations 1 to 5 and 7 to 8, follow the procedure below to do so: 1. Select the sample results of locations 1 to 5 as instructed in steps 1 to 4 of Example1; 2. Select the sample results of locations 7 to 8 as instructed in steps 1 to 4 of Example1; 3. CLICK Exit to return to the sample table review screen. The selected sample results will be marked with *, as Figure 7-30 shows.
Figure 7-30 Reviewing the selected results Example4: To deselect the sample results of locations 1 to 5 and 7 to 8, follow the procedure below to do so: 1. Deselect the sample results of locations 1 to 5 as instructed in steps 1 to 3 of Example2; 2. Deselect the sample results of locations 7 to 8 as instructed in steps 1 to 3 of Example2;
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Reviewing Sample Results 3. CLICK Exit to return to the sample table review screen. The * above those sample results will disappear, as Figure 7-31 shows.
Figure 7-31 Reviewing the deselected results
Transmitting sample results to a host

You can transmit the selected or all sample results to an external computer (a host). Press [F3] to enter the Transmit window, as Figure 7-32 shows.
Figure 7-32 Transmit screen To transmit the selected sample results to a host, CLICK Selected; To transmit all the sample results, CLICK All; To stop a transmission, CLICK Stop; To return to the review screen, CLICK Exit.
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7.3.3 Reviewing Search Result in the Histogram Mode

Entering the Histogram mode
At the Review screen, press [] to select the Searched database and then press [] to enter the Histogram screen, as Figure 7-34 shows.
Figure 7-34 Searched histogram screen

Press [] or [] to browse the preceding or following sample result; press [PgUp] or [PgDn] to jump 8 locations (e.g. jumping from location 1 to location 8 ).
Switching to the Table mode

To switch to the Table mode, press []; to switch back to the Histogram mode, press 7-19
Reviewing Sample Results [] again.

Editing sample information

Press [F2] to edit the sample information, as Figure 7-36 shows.
Figure 7-36 Editing sample information ID You cannot edit the sample ID of an analyzed sample. Selecting the animal gender SELECT the desired item from the Gender pull-down list. Note that you can select blank in case you are not aware of the animal gender. Entering the owners name ENTER the owners name into the Owner box. Entering the animal name ENTER the animal name into the Animal box.
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Reviewing Sample Results Entering the animal age This analyzer provides three ways for you to enter the animal age in years, in months and in days. The first way is designed for the animals no younger than one year; the second for the animals older than one month and younger than one year; the third for the animals younger than one month. You can choose only one of the three ways to enter the animal age. To enter the patient age in years: ENTER the desired number, an integer from 0 to 200, into the Years box. To enter the patient age in months: ENTER the desired number, an integer from 0 to 12, into the Months box. To enter the patient age in days: ENTER the desired number, an integer from 0 to 31, into the Days box. Yes button When you have finished entering all the desired sample information, CLICK the Yes button to save the changes and return to exit the edit window. No button If you do not want to save the entered information, CLICK the No button to return to exit the edit window.

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8
8.1 Introduction
Using the QC Programs
Quality Control (QC) consists of strategies and procedures that measure the precision and stability of the analyzer. The results imply the reliability of the sample results. QC involves measuring materials with known, stable characteristics at frequent intervals. Analysis of the results with statistical methods allows the inference that sample results are reliable. Mindray recommends you run the QC program daily. A new lot of controls should be analyzed in parallel with the current lot prior to their expiration dates. This may be accomplished by running the new lot of controls twice a day for five days using any empty QC files. The QC files calculate the mean, standard deviation and coefficient of variation for each selected parameter. The instrument-calculated means of these ten runs should be within the expected ranges published by the manufacturer.
Use the specified controls. Using controls other than the specified will lead to misleading results. Refer to the instructions of use of the controls for how to store and use the controls.
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8.2 QC Editing
8.2.1 Entering the Quality control screen
Press [MENN] to enter the system menu. SELECT Quality control (Figure 8-1), to enter the Quality control screen, as Figure 8-2 shows. At the QC screen you can include a maximum of 8 parameters, WBC, RBC, HGB, PLT, HCT, MCV, MCHC and MCH into a QC run.
Figure 8-2 QC menu
8.2.2 Selecting a QC File

The analyzer provides 9 QC files for you to save QC settings and results. Every QC file can save the results of a maximum of 31 QC runs. When the saved QC results have reached the maximum number, the newest result will overwrite the oldest. You can press [F1] to switch the QC files and the number will be given on the upper left of the screen.
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8.2.3 Editing L-J Settings

If there are saved L-J results and settings, you need to delete them first. You can press [F5] to enter QC Table screen to delete all the results, see Chapter 8.4.2 QC Table for details. Entering the QC Edit screen Press [F3] at Quality control screen to enter the QC Edit screen (Figure 8-3).
Figure 8-3 QC Edit screen Entering lot number ENTER the lot number of the control to be used into the Lot No. box, as Figure 8-4 shows.
Figure 8-4 Entering lot number Entering expiration date ENTER the expiration date of the control to be used into the Exp. Date box, as Figure 8-5 shows.
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Figure 8-5 Entering Exp. date Entering the expected results (mean) and limits (range) ENTER the expected results (mean) and limits (range) respectively into the Mean and Range boxes of the parameters to be included in the L-J analysis, as Figure 8-6 shows.
Figure 8-6 Entering Mean of RBC
Refer to the instructions of use of the control for information on the lot number, expiration date, open-vial stability days, expected results and limits. The entered expiration date should be either the expiration date printed on the labeling or the open-vial expiration date, whichever is earlier. The open-vial expiration date is calculated as follows: the date that vial is opened + the open-vial stability days. At the QC Edit screen, if you want to correct an erroneous entry, MODIFY the wrong digit.
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Using the QC Programs Deleting settings Press [DEL] to delete all the settings. Printing settings Press [PRINT] to print out all the settings. Exiting the QC Edit screen Press [MENU] to exit to the system menu. A message box shown in Figure 8-7 will pop up, if : 1. There is a parameter for which you have entered only the expected result or the limit; or 2. There is a parameter whose expected result is less than or equal to the limit.
Figure 8-7 invalid input CLICK Yes to close the box and clear the erroneous entries. Re-enter the correct values before trying to exit the screen again. The settings can be saved only when both the expected result and limit are valid. If all the entries are correct, a message box will pop up to remind you to save the changes, as Figure 8-8 shows. CLICK Yes to save the changes and exit to the Quality control screen; CLICK No to abort the changes and exit to the Quality control screen.
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8.3 Running the Controls
8.3.1 Whole Blood Mode

1. Be sure the System Status area displays Ready; 2. Be sure the Analysis Mode area displays WB. If not, press [F2] to select the whole blood mode; 3. Present a vial of control to the sample probe so that the tip is well into the vial, and press the aspirate key. The System Status area will display Running and the analyzer will start aspirating sample; 4. When you hear a beep and the sample probe is out of the vial, remove the vial. The sample probe will retract into the analyzer and the analysis progress will be displayed on the screen; 5. When the analysis is finished, the result will be displayed on the screen and the NO./Total in the upper left corner of the screen will automatically increase by 1 and the 8-6
Using the QC Programs sample probe will be repositioned.
If the analyzer detects WBC/RBC clogging or bubbles during the analysis, the corresponding error messages will be displayed in the lower left corner of the screen and the results of all the related parameters will be invalidated. See Chapter 11 Troubleshooting Your Analyzer for solutions. If the ambient temperature is outside the specified operating range, the analyzer will alarm you for abnormal ambient temperature and the analysis results may be unreliable. See Chapter 11 Troubleshooting You Analyzer for solutions.
Deleting the QC Result To delete the current result, press [DEL] and a message box will pop up, as Figure 8-9 shows. CLICK Yes to confirm the deletion.
Figure 8-9 Deleting current result Printing QC results Press [PRINT] to print out the current QC result by the printer. Exiting the Quality control screen Press [MENU] to exit to the system menu.
8.3.2 Prediluted Mode

1. Be sure the System Status area displays Ready; 2. Be sure the Analysis Mode area displays PB. If not, press [F2] to select the prediluted blood mode; 3. Press [DILUENT] and a message box will pop up to instruct you how to dispense the diluent into the sample tube, as Figure 8-10 shows;
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Figure 8-10 A message box showing you how to dispense diluent 4. Present a clean sample tube to the sample probe and make sure the tube is tilted towards the probe, as Figure 8-11 shows, to avoid spills and bubbles. Press the aspirate key to dispense 0.7ml of diluent (the dispensing volume is controlled by the analyzer) into the tube;
Figure 8-11 How to dispense diluent 5. When the dispensing is finished, press [ENTER] to close the message box; 6. Add 20L of control to the diluent and shake the tube to mix the sample; 7. Present the mixed control to the sample probe so that the tip is well into the tube, and press the aspirate key. The System Status area will display Running and the analyzer will start aspirating sample; 8. When you hear the beep and the sample probe is out of the tube, remove the sample tube. The sample probe will retract into the analyzer and the analysis progress will be displayed on the screen; 9. When the analysis is finished, the result will be displayed on the screen and the NO./Total in the upper left corner of the screen will automatically increase by 1 and the sample probe will be repositioned.
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If the analyzer detects WBC/RBC clogging or bubbles during the analysis, the corresponding error messages will be displayed in the lower left corner of the screen and the results of all the related parameters will be invalidated. See Chapter 11 Troubleshooting Your Analyzer for solutions. If the ambient temperature is outside the specified operating range, the analyzer will alarm you for abnormal ambient temperature and the analysis results may be unreliable. See Chapter 11 Troubleshooting You Analyzer for solutions.
Deleting the QC Result To delete the current result, press [DEL] and a message box will pop up, as Figure 8-12 shows. CLICK Yes to confirm the deletion.
Figure 8-12 Deleting current result Printing QC results Press [PRINT] to print out the current QC result by the printer. Exiting the Quality control screen Press [MENU] to exit to the system menu.
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8.4 Reviewing QC Results

You can review the saved results in either of the two modes L-J Graph and QC Table.
8.4.1 L-J Graph

At the Quality control screen, press [F4] to enter the L-J Graph screen, as Figure 8-13 and Figure 8-14 shows.
Figure 8-13 L-J graph screen (1)
Figure 8-14 L-J graph screen (2) The 8 parameters are divided into 2 groups for display, one group for one screen. You can press [] or [] to switch among the screens. At every L-J Graph screen, you can press [] or [] to view the results (displayed below the parameter box) of every point presented in the 8-10
Using the QC Programs graph. The current cursor position is displayed to the right of No. field and the time at which this QC run was done is displayed to the right of Time field. The L-J graph is interpreted as follows: The x-coordinate represents how many times the QC program has been run. The y-coordinate represents the analysis results of the displayed parameters. For every parameter, its L-J graph presents a maximum of 31 points. For every parameter, the upper dash line of its L-J graph represents the upper limit of the expected range of the analysis result. The corresponding value (4.9 in case of the WBC in Figure 8-13) equals Mean + Range and is displayed to the left of the line. For every parameter, the lower dash line of its L-J graph represents the lower limit of the expected range of the analysis result. The corresponding value (4.1 in case of the WBC in Figure 8-13) equals Mean - Range is displayed to the left of the line. For every parameter, its expected result (4.5 in case of the WBC in Figure 8-13) is displayed between the values of the upper dash line and of the lower dash line. For every parameter, the three numbers displayed to the right of its L-J graph represents: Mean the mean value of the saved results, as the equation below defines,
Mean =
X
i =1
where n represents how many times the QC program has been run and Xi is the result acquired from every QC analysis. Diff standard deviation of the saved analysis results, as the equation below defines,
X i Mean Diff = n 1
)2
where n represents how many times the QC program has been run and Xi is the result acquired from every QC analysis and Mean is the mean value derived from the first equation. CV Coefficient of Variation, as the equation below defines
CV =
Diff 100% Mean
where Mean is the mean value derived from the first equation and Diff is the standard deviation derived from the second equation. Every point in the graph is interpreted as follows: 8-11
Using the QC Programs The darkened square that falls between the upper and the lower dash lines is within the control range. Otherwise, it is not. The blank square represents the QC analysis either ran into errors or is out of the display range. If you see any points fallen outside the control range, do the following steps until the problem is solved. If all the steps have failed, contact Mindray customer service department or your local distributor for assistance. 1. Check the lower left corner of the screen for error messages. Refer to Chapter 11 Troubleshooting Your Analyzer for solutions to any displayed error messages; 2. Check the L-J settings for inappropriate entries; 3. Do the background check. In case of an abnormal background result, refer to Chapter 11 Troubleshooting Your Analyzer for solutions; 4. Re-run the control; 5. Run another vial of control; 6. Check if the analyzer needs to be calibrated.
Other operations: To print out the currently displayed L-J graph, press [PRINT]. To acquire help information, press [HELP]. To return to the Quality control screen, press [MENU].
8.4.2 QC Table
At the Quality control screen, press [F5] to enter the QC Table screen, as Figure 8-15 shows, where every screen displays the results of 6 QC analyses. You can press [PgUp] or [PgDn] to switch to the previous or next screen to view other results.
Figure 8-15 QC Table screen 8-12
Using the QC Programs If you want to delete all the saved results, press [DEL] and a message box will pop up to confirm the deletion, as Figure 8-16 shows.
Figure 8-16 Deleting all the result CLICK Yes to delete current result; CLICK No to abort the deletion. If you want to transmit the saved QC results to an external computer, follow the steps given below: 1. Press [F1] at QC Table screen to enter the dialog box shown in Figure 8-17; 2. SELECT Yes to confirm the transmission.
Figure 8-17 Transmission dialog box
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Using the Calibration Programs
9.1 Introduction
The purpose of the calibration is to maintain system accuracy. Quality of the calibration depends on the calibration materials and reagents used. You should only use the calibrators and reagents specified by Mindray for the calibration. Store and use the calibrators and reagents as directed by their instructions for use.
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9.2 When to calibrate

You should run the calibration program if the quality control results indicate there may be a problem.
All of the measured parameters must be calibrated before readings of this analyzer can be used as valid analysis results.
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9.3 How to Calibrate

The analyzer provides 2 calibration programs: manual calibration and auto calibration using commercial calibrators. In addition, every type of animal has its own set of calibration factors.
9.3.1 Preparing Your Analyzer

Do the following pre-calibration procedures before calibration. If problems are detected during these checks, do not attempt to calibrate the analyzer. If necessary, call Mindray customer service department or your local distributor for assistance. Check and make sure there are enough reagents for the calibration. Do the background check. If the analyzer alarms for abnormal background results, see Chapter 11 Troubleshooting Your Analyzer for solutions. It is recommended that you create a log table for your analyzer. This log table should contain all necessary information that is pertinent to your analyzer. Suggested items that you may want to include in the log table are: Calibration date Supplier of calibrator Lot number Expected results and limits Result of background check. Enter the administrator password as instructed in Chapter 5.2.1 and then choose one or several parameters among WBC, RBC, HGB, MCV and PLT for calibration.
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9.3.2 Auto Calibration Program

The new calibration factors obtained though auto calibration will be applied to all animal species. Press [MENU] to enter the system menu.
Figure 9-1 System menu SELECT Calibration Auto (Figure 9-1) to enter the Auto screen (Figure 9-2).
Figure 9-2 Auto screen
Selecting the count mode

Press [F1] to select desired calibration mode.
Editing calibration settings

Press [F2] to activate the edit boxes. Entering lot number 9-4
Using the Calibration Programs ENTER the lot number of the calibrator to be used into the Lot No. box. Entering Exp. Date ENTER the expiration date of the calibrator to be used into the Exp. Date box. Entering the expected results (mean) and limits (range) ENTER the expected results (mean) into the Mean box of the parameters to be included in the calibration.
Refer to the instructions of use of the calibrators for information on the lot number, expiration date, expected results and limits. Open reagents are stable for 60 days. The entered expiration date should be the open date + 60 days or the expiration date marked on the packaging of the reagent, whichever is earlier. When editing the settings, if you want to correct an erroneous entry, MODIFY the wrong digit.
Exit editing When you have finished editing the desired settings, press [F2] to deactivate the edit boxes.
Running the calibrator
Use the Mindray- specified calibrator. Using calibrator other than the specified will lead to misleading results. Refer to the instructions of use of the calibrator for how to store and use the calibrator. In the prediluted calibration mode, you cannot dispense diluent from the analyzer. It is recommended that you prepare at least 7 cups of diuent before starting calibrating the analyzer in the prediluted mode. Keep the sample probe tip away from the tube bottom; otherwise the aspiration volume may be inaccurate. When the aspiration is done, remove the sample tube only when the sample probe is out of the tube.
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The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it. Do not re-use such disposable products.
In the whole blood mode 1. At Auto screen, press [F1] to select Whole Blood mode; 2. Present a vial of mixed calibrator to the sample probe so that the tip is well into the tube, and press the aspirate key and the analyzer will start aspirating sample; 3. When you hear the beep and the sample probe is out of the vial, remove the calibrator. The sample probe will retract into the analyzer and the analysis progress will be displayed on the screen; 4. When the analysis is finished, the result will be displayed on the screen and the sample probe will be repositioned.
In the prediluted mode 1. Press [MENU] and SELECT Count to enter the Count screen; 2. At Count screen, be sure the System Status area displays Ready and the Count Mode area displays PB; 3. Press [DILUENT] and a message box will pop up to instruct you how to dispense the diluent into the sample tube, as Figure 9-3 shows;
Figure 9-3 Add Diluent window 4. Present a clean sample tube to the sample probe and make sure the tube is tilted towards the probe, as Figure 9-4 shows, to avoid spills and bubbles. Press the aspirate key to dispense 0.7mL of diluent (the dispensing volume is controlled by the analyzer) 9-6
Using the Calibration Programs into the tube. Prepare at least 7 cups of diluent;
Figure 9-4 How to dispense diluent 5. When the dispensing is done, press [ENTER] to close the message box; 6. Make sure the calibrator is at room temperature and not expired; 7. Add 20L of calibrator into one of the prepared sample cups and mix them well; 8. Present the mixed calibrator to the sample probe; 9. Press the aspirate key to start the run. When you hear a beep and the sample probe is out of the cup, remove the sample; 10. Press [MENU] and SELECT Calibration Auto; 11. Press [F1] to select the Prediluted mode; 12. Press [F2] to activate the edit boxes and edit the settings as needed. When the editing is done, press [F2] again to deactivate the edit boxes; 13. At the Auto screen, repeat steps 6 to 9 for 5 consecutive times.
Keep dust from the prepared diluent. Evaluate predilute stability based on your laboratorys sample population and sample collection techniques or methods. If the analyzer detects WBC/RBC clogging or bubbles during the analysis, the corresponding error messages will be displayed and the results of all the related parameters will be invalidated. See Chapter 11 Troubleshooting Your Analyzer for solutions. If the ambient temperature is outside the specified operating range, the analyzer will alarm you for abnormal ambient temperature and the analysis results may be unreliable. See Chapter 11 Troubleshooting Your Analyzer for solutions.
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Saving the calibration results

If non-numeric parameter values (***) are obtained, a message box will pop up to warn you, as Figure 9-5 shows. CLICK Yes to close the dialog box and discard the result.
Figure 9-5 A message box to warn you about the invalid results If the parameter values obtained are numeric, a message box will pop up to ask you to confirm whether the obtained calibration result is valid. Figure 9-6 shows the message box asking whether you think the result of the first calibration (calibration 1) is valid..
Figure 9-6 A message box to confirm the validity You can either CLICK Yes to save the results, or CLICK No to abort the result. The saved results will be displayed on the screen. Repeat the above steps to run the calibrator 3 to 5 times (5 is recommended) and the analyzer will automatically calculate the CVs and calibration factors, as Figure 9-7shows.
Figure 9-7 Results of the auto calibration The calculated calibration factor should be within the 75 to 125. If not, there will be flagged with a *. Other values will not be displayed. In case of an empty calibration factor, try to find out the reason and if necessary, contact Mindray customer service department or your local distributor for assistance. 9-8
Using the Calibration Programs Press [MENU] to exit the Auto screen, a message box will pop up to confirm the new calibration factors, as Figure 9-8 shows.
Figure 9-8 A message box to confirm the new calibration factors CLICK Yes to save the new calibration factors. Click No to close the box without saving the changes. .
Other operations
Printing new calibration factors Press [PRINT] to print out the current calibration factors. Exiting the Auto screen Press [MENU] to exit to the system menu
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9.3.3 Manual Calibration Program

You should first enter the administrator password 2826at the Setup Password screen.
Selecting animal
Every type of animal has its own set of calibration factors. Select the right animal type before proceeding with the calibration. Press [MENU] to enter the system menu, as Figure 9-9 shows;
Figure 9-9 System menu SELECT Animal to enter the Animal screen, as Figure 9-10 shows;
Figure 9-10 Animal screen SELECT the desired animal;
Editing the new calibration factors

Press [MENU] to enter the system menu.
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Figure 9-10 System menu SELECT Calibration Manual (Figure 9-10) to enter the Manual screen ( Figure 9-11).
Figure 9-11 Manual screen
The left of the Manual" screen displays the available calibration modes Whole blood and Prediluted. The right of the Manual screen displays the calibration factors of WBC, RBC, HGB, MCV, PLT and the time the factors are saved. Press [F2] to activate the edit boxes as Figure 9-12 shows.
Figure 9-12 Edit boxes activated ENTER the new calibration factor into the corresponding boxes.
Exit editing
Press [F2] to exit editing. If the entered number is out of the calibration range, a dialog box will pop up to remind you the entered number is invalid, as Figure 9-13 shows. 9-11
Figure 9-13 A message box to warn invalid input Seeing the box, CLICK Yes and re-enter the factors. If the changed factors are all within the calibration range, a dialog box will pop up to remind you to save the new factors, as Figure 9-14 shows.
Figure 9-14 A message box to confirm the new calibration factors CLICK Yes to save the new calibration factors, and then a dialog box will pop up to ask you whether to apply the factors to all animal species, as Figure 9-15 shows.
Figure 9-15 A message box to confirm the application of the new calibration factors Click Yes to apply the new calibration factors to all animal species. Click No to close the box without applying.
Other operations
Printing new calibration factors 9-12
Using the Calibration Programs Press [PRINT] to print out the current calibration factors. Exiting the Manual screen Press [MENU] to exit to the system menu.
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10 Maintaining Your Analyzer

10.1 Introduction
Preventive and corrective maintenance procedures are required to keep the BC-2800Vet in a good operating condition. This analyzer provides multiple maintenance functions for this purpose. This chapter introduces how to use the provided functions to maintain and troubleshoot your analyzer.
Do not perform any maintenance procedures that are not described in this chapter. Performing unauthorized maintenance procedures can damage your analyzer. In case of problems not specified in this manual, contact Mindray customer service department or your local distributor for assistance. Only Mindray-supplied parts can be used for maintenance. For any questions, contact Mindray customer service department or your local distributor.
10-1
Maintaining Your Analyzer
10.2 General Guidelines

Maintenance Period Everyday Content of Maintenance If you are to use this analyzer 24 hours a day, perform the E-Z cleanser cleaning procedure everyday. Run the QC program everyday. See Chapter 8 Using the QC Programs for details. Every three days Every Week If you are to use this analyzer 24 hours a day, perform the Probe cleanser cleaning procedure every three days. If you shut down your analyzer every day and follow the specified shutdown procedure to do that, you need to perform the Probe cleanser cleaning procedure every week. Every Month You should use the supplied probe localizer to calibrate the position of the probe to that of the probe wipe. The analysis result is sensitive to their alignment. As needed When you think the bath might be contaminated, perform the Clean the bath procedure. When the analyzed samples add up to 100, the analyzer will remind you to perform the Probe cleanser cleaning procedure. When the analyzed samples add up to 100, the analyzer will remind you to perform the E-Z cleanser cleaning procedure. When the analyzed samples add up to 4,000, the analyzer will remind you to perform the Clean wipe block procedure. When this analyzer is not to be used for two weeks, perform the Prepare to ship procedure to empty and wash the fluidic lines and then wipe the analyzer dry and wrap it up for storage. To obtain reliable analysis results, this analyzer needs to work in a normal status. Run the Self-test items regularly to check the status of this analyzer. When this analyzer gives alarms for clogging, you can perform the Flush aperture or Zap aperture procedure, or press [F2] to unclog the aperture. If you see other error messages, see Chapter 11 Troubleshooting Your Analyzer for solutions.
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10.3 Using the Maintenance Program

Press [MENU] to enter the system menu. SELECT Service Maintenance (Figure 10-1) to enter the Maintenance screen (Figure 10-2).
Figure 10-2 Maintenance screen
10-3
There are 13 maintenance procedures available at the Maintenance screen. Diluent Prime Rinse Prime Lyse Prime Zap Aperture Flush Aperture Probe Cleanser Cleaning E-Z Cleanser Cleaning Lyse Test Clean Bath Drain Bath Drain Tubing Clean Wipe Block Prepare to ship
10.3.1 Diluent Prime
The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor.
After installing new reagents, let them stand for a while before using them. After installing a new container of diluent, rinse or lyse, do a background check to ensure the background results are normal.
You should perform the Diluent prime procedure to prime the diluent tubing when there are bubbles in the tubing; or
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Maintaining Your Analyzer the diluent in the tubing is contaminated; or you have installed a new container of diluent without shutting off the analyzer. At the Maintenance screen, SELECT Diluent prime to prime the tubing and the priming progress will be displayed at the bottom of the screen, Figure 10-3 shows. When the priming is done, the screen will return to the initial state.
Figure 10-3 Priming diluent screen
10.3.2 Rinse Prime
You should perform the Rinse prime procedure to prime the rinse tubing when 10-5
Maintaining Your Analyzer there are bubbles in the tubing; or the rinse in the tubing is contaminated; or you have installed a new container of rinse without shutting the analyzer. At the Maintenance screen, SELECT Rinse prime to prime the tubing and the priming progress will be displayed at the bottom of the screen, as Figure 10-4 shows. When the priming is done, the screen will return to the initial state.
Figure 10-4 Priming rinse screen
10.3.3 Lyse Prime
You should perform the Lyse prime procedure to prime the lyse tubing when
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Maintaining Your Analyzer there are bubbles in the tubing; or the lyse in the tubing is contaminated; or you have installed a new container of lyse without shutting the analyzer. At the Maintenance screen, SELECT Lyse prime to prime the tubing and the priming progress will be displayed at the bottom of the screen, as Figure 10-5 shows. When the priming is done, the screen will return to the initial state.
Figure 10-5 Priming lyse screen
10.3.4 Zap Aperture

You can perform the Zap aperture procedure to unclog the apertures or prevent clogging. At the Maintenance screen, SELECT Zap aperture to zap the apertures and the zapping progress will be displayed at the bottom of the screen, as Figure 10-6 shows. When the zapping is done, the screen will return to the initial state.
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Figure 10-6 Zapping aperture
10.3.5 Flush Aperture

You can perform the Flush aperture procedure to flush the aperture to unclog the aperture or prevent clogging. At the Maintenance screen, SELECT Flush aperture to flush the aperture and the flushing progress will be displayed at the bottom of the screen, as Figure 10-7 shows. When the flushing is done, the screen will return to the initial state.
Figure 10-7 Flushing aperture
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10.3.6 Probe Cleanser Cleaning
The probe cleanser is corrosive. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.
You can soak the bath and fluidic lines with the probe cleanser, an alkaline detergent, by performing the Probe cleanser cleaning procedure. If your analyzer is to run 24 hours a day, you should perform this procedure every 3 days. If you follow the shutdown procedure to turn off your analyzer everyday, you should perform this procedure every week. Follow the steps given below to do so: 1. At the Maintenance screen, SELECT Probe cleanser cleaning; 2. Present the cleanser to the probe and press [ENTER] to aspirate the cleanser. When you hear the beep and the sample probe is out of the bottle, remove the cleanser. The analyzer will start priming process, as Figure 10-8 shows;
Figure 10-8 Priming bath and fluidic lines 3. When the priming is done, the analyzer will start the 5-minute soaking process, as Figure 10-9
Maintaining Your Analyzer 10-9 shows and you may press [ENTER] to stop it before the time is due. Note that a shortened priming process may not be as effective as a complete one;
Figure 10-9 soaking process 4. When the soaking is done, the analyzer will start the cleaning process, as Figure 10-10 shows, after which screen will return to the initial state;
Figure 10-10 Cleaning process To make sure this analyzer functions normally, every time the accumulated analyzed samples reach 100, a message box will pop up to remind you to perform the probe cleanser cleaning procedure, as Figure 10-11 shows. CLICK Yes to proceed with the cleaning; CLICK No to cancel the cleaning.
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Figure 10-11 A message box to confirm the cleaning
10.3.7 E-Z Cleanser Cleaning
You can use the E-Z cleanser, an enzyme based, isotonic cleaning solution and wetting agent, to clean the tubing and bath by performing the E-Z Cleanser Cleaning procedure. Follow the steps given below to perform the procedure: 1. At the Maintenance screen, SELECT E-Z Cleanser Cleaning; 2. Present the cleanser to the probe and press [ENTER] to aspirate the cleanser. When you hear the beep and the sample probe is out of the bottle, remove the cleanser. This analyzer will automatically prime the bath and fluidic lines with the aspirated cleanser and the progress is displayed on the screen, as Figure 10-12 shows;
Figure 10-12 Priming the bath and fluidic lines 3. When the priming is done, the analyzer will start the 10-minute soaking process, as Figure 10-13 shows. 10-11
Figure 10-13 E-Z cleaning 4. When the soaking is done, the analyzer will start the draining process, as Figure 10-14 shows. When the draining is done, the whole procedure is over and the screen will return to the initial state.
Figure 10-14 Draining the bath and fluidic lines If your analyzer has been running continuously for 24 hours, a dialog box, as Figure 10-15 shows, will pop up to remind you to perform the E-Z cleanser cleaning procedure. If you want to do so, CLICK Yes . Otherwise, CLICK No to cancel the cleaning.
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Figure 10-15 A message box to confirm the cleaning
10.3.8 Lyse Test
In case of any abnormal WBC counts or histograms, you can perform the Lyse test procedure to check whether the lyse can be dispensed properly. Follow the steps given below to do so: 1. Unscrew and remove the retaining screws (pointed to by the arrows shown in Figure 10-16) on the right plate;
Figure 10-16 Removing the two screws 2. Follow the arrow shown in Figure 10-17 to push and remove the right plate; 10-13
Figure 10-17 Removing right plate 3. Remove the screws fixing the shielding box of the bath, as Figure 10-18 shows;
Figure 10-18 Shielding box 4. Remove the shielding box to expose the bath, as Figure 10-19 shows;
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Figure 10-19 Bath 5. SELECT Lyse test. The analyzer will automatically drain the bath and then dispense 2ml of lyse into the bath; 6. Check the scale to see whether the lyse has reached the expected line (the second from the bottom). If so, press [ENTER] and the analyzer will automatically flush the bath and dispense lyse and the test is done; 7. If not, repeat steps 5 and 6 several times. If all the tries have failed, check whether the lyse has run out or the lyse pickup tube is not properly connected to this analyzer. If there is still enough lyse and the tube is well connected to the analyzer, contact the Mindray or your local distributor for assistance.
10.3.9 Clean Bath

Follow the steps given below to perform the Clean bath procedure: SELECT Clean bath to start the cleaning procedure, as Figure 10-20 shows. When the cleaning is done, the screen will return to the initial state;
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Figure 10-20 Clean bath
10.3.10 Drain Bath

When three or more of the WBC, RBC, PLT and HGB results are abnormal, you may do the Drain bath procedure to determine the reason. Follow the steps below to do so: 1. Do steps 1 to 4 of the Lyse test procedure to expose the bath; 2. SELECT Drain bath to drain the bath;
Figure 10-21 Draining the bath 3. When the draining is done, check the bath and the tubing below for residual fluid. If there is no fluid, press [ENTER] to prime the bath with diluent, as Figure 10-22 shows. To prevent you from forgetting to prime the bath, a time box will pop up as a reminder;
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Figure 10-22 The time of draining bath
Figure 10-23 Priming the bath with diluent
4. When the priming is done, the screen will return to the initial state; 5. If there is fluid left, turn off the analyzer and call Mindray customer service department or your local distributor for assistance.
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10.3.11 Drain Tubing
Do the Drain Tubing procedure before relocating the analyzer.
You can perform the Drain tubing procedure to drain the fluidic system. Follow the steps given below to do so: 1. Press the appropriate arrow keys ([][] [][]) as needed to move the cursor to Drain tubing; 2. Remove the diluent, rinse and lyse pickup tubes from the back of the analyzer; 3. Press [ENTER] to start the draining process, as Figure 10-24 shows;
Figure 10-24 Draining the fluidic lines 4. When the draining is done, the screen will display Turn off this analyzer and you should turn off the power switch. 10-18
10.3.12 Clean Wipe Block

After being used for a long time, the bottom of the probe wipe may be contaminated by blood and the inside of the probe wipe may also be contaminated by the dirt sucked in. So you need to clean the probe wipe regularly. Follow the steps given below to do so: 1. Present the probe cleanser to the sample probe; 2. SELECT Clean wipe block to start the aspiration; 3. When you hear the beep and the sample probe is out of the bottle, remove the cleanser; 4. Unscrew and remove the retaining screws (pointed to by the arrows shown in Figure 10-25) on the right plate;
Figure 10-25 Removing the two screws 5. Follow the arrow shown in Figure 10-26 to push and remove the right plate;
Figure 10-26 Removing right plate 10-19
Maintaining Your Analyzer 6. Follow the instructions displayed on the screen to place an empty cup, whose diameter should be no less than 8cm, below the sample probe; 7. Press [ENTER] to soak the wipe block with the aspirated cleanser. The soaking progress will be displayed on the screen, as Figure 10-27 shows;
Figure 10-27 Cleaning wipe block 8. When the soaking is done, wipe the bottom of the wipe block with a probe cleanser-dipped cloth that does not leave debris; 9. Press [ENTER] to flush the block and the interior of the probe and the flushing progress is displayed on the screen, as Figure 10-28 shows;
Figure 10-28 Flush the block and the interior of the probe 10. After the flushing is done, the screen returns to the initial state. 10-20
When the accumulated analyzed samples reach 4,000, a message box will pop up to remind to clean the probe wipe, as Figure 10-29 shows. CLICK Yes to do the procedure; CLICK No to abort the procedure.
Figure 10-29 message box
10.3.13 Prepare to ship

Use the Prepare to ship program to prepare your analyzer for a prolonged period of non-use or for shipping. Follow the steps given below to do so: 1. Press the appropriate arrow keys ([][][][]) to move the cursor to Prepare to ship. Remove the diluent, rinse and lyse tubing from the containers following the instructions displayed on the screen; 2. Press [ENTER] and a message box will pop up to ask you to confirm this operation, as Figure 10-30 shows;
Figure 10-30 A message box of prepare to ship 3. CLICK No if you want to abort this operation; CLICK Yes to proceed with the operation. The analyzer starts to drain the fluidic lines and the progress is displayed on the screen, as Figure 10-31 shows.
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Figure 10-31 Draining fluidic lines 4. After draining the tubing, follow the instructions displayed on the screen (Figure 10-32) to put the rinse, diluent and lyse tubing into distilled water and press [ENTER] to flush this analyzer with the distilled water;
Figure 10-32 Washing the analyzer 5. When the washing is over, follow the instructions displayed on the screen to remove the rinse, diluent and lyse tubing from the distilled water and press [ENTER] to drain the tubing again; 6. Turn off the analyzer when the screen displays Turn off the analyzer; 7. Wipe this analyzer dry and wrap it up for storage.
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10.4 Using the Status Program

The items displayed in the System Status screen reflect how the analyzer is functioning and contribute significantly to diagnosing analyzer errors. You may follow the instructions given below to check those items. Press [MENU] to enter the system menu and SELECT Service Status, as Figure 10-33 shows, to enter the Status screen, as Figure 10-34 shows.
Figure 10-34 Status screen At the Status screen you can only view the displayed status information and reference ranges.
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10.5 Using the Self-test Program

The system self-test is a major way to locate system errors. Follow the instructions given below to view and check the available self-test items. Press [MENU] to enter the system menu and SELECT Service Self-test, as Figure 10-35 shows, to enter the Self-test screen, as Figure 10-36 shows.
Figure 10-36 Self-test screen
This screen can be interpreted as follows: Test Groups area (on the left) This area displays the test groups. The available self-test items are divided into four groups, Tubing, Machine, Valve and Circuit. Press [F1] to select the desired group. The selected group is preceded by a .
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Maintaining Your Analyzer Test Result area (on the right) This area displays the items included in the test group and the test results. Help area (on the bottom) This area displays useful information to help you move to the next step. At this screen, if you want to acquire help information, press [HELP]; if you want to print out the test results (except for the results of the valve tests), press [PRINT].
10.5.1 Testing the fluidic system

Press [F1] to select the Tubing group. To conduct the following tests, just SELECT the desired test and the results will be displayed later. Count Time It measures the duration of a WBC and RBC count, namely how many seconds it takes for the aspirated fluid flows from the first sensor to the second. Aperture(v) It measures the voltage (v) over the aperture. Vacuum It checks whether the vacuum system functions normally. Pressure It checks whether the system flushes the aperture at a normal pressure. Filter It checks whether the filter functions normally.
10.5.2 Testing Motors and Recorder/Printer

To test the motors and recorder/printer, press [F1] to select the Machine group, as Figure 10-37 shows.
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Figure 10-37 testing mechanic part To conduct the following tests, SELECT the desired test and the results will be displayed later. Syringe motor The syringe motor controls the aspiration volume. This test checks whether the motor functions normally. Rotation motor The rotation motor rotates the sample probe inside the analyzer. This test checks whether the motor functions normally. Elevator motor The elevator motor controls elevation of the sample probe. This test checks whether the motor functions normally. Print This test checks whether the recorder or printer functions normally. If normal, when you press [ENTER], the recorder or printer will print out a test page; if abnormal, the screen will display the corresponding error message and you can see Chapter 11 Troubleshooting Your Analyzer for solutions.
10.5.3 Testing Valves

To test whether the valves function properly, press [F1] to select the Valve group, as Figure 10-38 shows. Follow the steps below to test the desired valve.
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Figure 10-38 Testing valves To test a valve, SELECT the valve. If the valve goes through an Off-On-Off sequence without making any abnormal sound, it passes the test. Otherwise, something may be wrong with the valve.
10.5.4 Testing A/D Interrupt

To test the A/D interrupt, press [F1] to select the Circuit group, as Figure 10-39 shows.
Figure 10-39 Testing A/D interrupt To conduct the test, SELECT A/D interrupt and the test result will be displayed later.
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10.6 Log
The log records all the major events taking place during the running of this analyzer. It helps the service engineers diagnose system errors. Press [MENU] to enter the system menu and SELECT ServiceLog, as Figure 10-40 shows to enter the Log screen, as Figure 10-41 shows.
Figure 10-41 Log screen The recorded events are divided into three groups, All, Settings and Other( including setting discriminators, system self-test and updating system software), which are all listed on the left of the screen. All the recorded events are listed on the right of the screen by default. You can press [F1] to select the specific group and the right of the screen will display the events of the selected group only. Every screen displays 10 events. You can press [] or [] to check the events one by one or press [PgUp] or [PgDn] to check the events on the previous or next screen. If you want to print out the displayed events, press [PRINT]. If you want to acquire help information, press [HELP]. 10-28
For every recorded event, the NO. column displays the sequences of the recorded events; the Time column displays the time when this event occurred; the Type column displays the event type; the Times column displays how many times (1 to 255) this event occurred and if it occurred more than 255 times, the excessive events will be recorded from 1 to another log file; the Information column displays extra information regarding the event. This analyzer can save a maximum of 1000 log files and once the maximum number has been reached, the newest log will automatically cover the oldest one.
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10.7 Viewing System Configuration

To view the system configuration, press [MENU] to enter the system menu, and SELECT ServiceConfig. , as Figure 10-42 shows, to enter the Config. screen, as Figure 10-43 shows.
Figure 10-42 system menu
Figure 10-43 Config. screen Every screen displays 13 items and you can press [] or [] to select the item you want to see, or press [PgUp] or [PgDn] to go to the previous or next screen. If you want to print out the configuration, press [PRINT]. If you want to acquire help, press [HELP].
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10.8 Printing Management

Press the [MENU] to enter the system menu and SELECT ServicePrint. , as Figure 10-44 shows, to enter the Print screen, as Figure 10-45 shows.
Figure 10-45 Print screen The printing tasks are queued in this screen, where you can view the all and delete those waiting to be processed. Once something goes wrong with the printing device, the task being processed will be deleted and the queued tasks will keep waiting. Once the system finds the error has been removed, it will resume printing and process the tasks from the first one. Note that you cannot change the sequence of the queued tasks. You can perform the following operations at the Print screen: Press [DEL] to delete the selected task; Press [HELP] to display the help information; Press [MENU] to return to the system menu.
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10.9 Calibrating Sample Probe Position
The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it.
The relative position between the sample probe and probe wipe block has influence on the analysis results. In the accessory box, there is a sample probe localizer, as Figure 10-46 shows. You need to use the localizer to adjust the position of the sample probe if you have replaced wipe block, or observed motor error, or incorrect result. Also, as required by regular maintenance, you should use the localizer to adjust the position of the sample probe monthly.
Figure 10-46 Probe localizer 1. SELECT Setup Password and enter the administrator password; 2. Unscrew and remove the retaining screws with hands or screwdrivers (pointed by the arrows shown in Figure 10-47) on the right plate.
Figure 10-47 Removing the two screws 3. Follow the arrow shown into push and remove the right plate; 10-32
Figure 10-48 Removing right plate 4. Press [F1] to select the Machine group and SELECT Elevator motor, as Figure 10-49 shows;
Figure 10-49 Self-test screen 5. An Elevator motor window will pop up, as Figure 10-50 shows;
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Figure 10-50 Elevator motor window 6. Press [] to move the sample probe upward and press [] to move the probe to above the bath, as Figure 10-51 shows;
Figure 10-51 Sample probe above the bath 7. Loose the retaining screw by a screwdriver, as Figure 10-52 shows.
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Figure 10-52 Removing screws 8. Remove the probe from the wipe block and insert the localizer into the wipe block from the bottom, as Figure 10-53 shows;
Figure 10-53 Using localizer 9. Insert the probe into the wipe block until it reaches the localizer, as Figure 10-54 shows;
Figure 10-54 Inserting sample probe into wipe block 10. Retighten the screw to fix the probe and remove the localizer to complete the adjustment.
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10.10 Replacing the Probe Wipe
The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it.
To replace the probe wipe: 1. Refer to Chapter 10.9 and do the steps 1 to 8; 2. Pull the loosen probe wipe upward to remove the wipe block and disconnect the tubes from the wipe block (pay attention to the correspondence between the tubes and the connectors), as Figure 10-55 shows;
Figure 10-55 Installing wipe block 3. Install a new block and connect the tubing end with the black marking to the connector below the block; 4. Calibration to the sample probe position should be performed after replacing the probe wipe. Refer to Chapter 10.9..
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10.11 Replacing the Filter of the Vacuum Chamber

1. Unscrew and remove the retaining screws with hands or screwdrivers (pointed by the arrows shown in Figure 10-56 ) on the right plate;
Figure 10-56 Removing the two screws 2. Follow the arrow shown in Figure 10-57 to push and remove the right plate;
Figure 10-57 Removing the right plate 3. Find the filter shows in Figure 10-58;
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Figure 10-58 Vacuum filter 4. Remove the filter and take a new one from the accessory kit and install it.
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10.12 Maintaining Recorder
Wear proper personal protective equipment (e.g. medical gloves, disposable wrist strap, etc.) against the static electricity during maintaining recorder. Make sure the power supply of the analyzer has been cut off before maintenance.
If the recorder finishes a print action, wait at least 20 minutes until the recorder head is cooled completely and then maintain the recorder. Do not add unnecessary force to the recorder head. Wipe off the alcohol remaining on the recorder head in time after cleaning the recorder head. Make sure the alcohol volatilizes completely before using the recorder to print.
Maintain the recorder strictly as instructed below.
You should maintain the recorder every two months. Do as follows to maintain your recorder: 1. Turn off the analyzer and cut off the power supply; 2. Open the recorder door and take out the recorder paper; 3. Gently wipe the roller from left to right using cotton swabs; 4. Roll the roller and repeat step 3 to clean off all debris and stains on the roller; 5. Gently wipe the heating part of the recorder head from left to right using cotton swabs dipped with alcohol (no drops) to clean off all debris and stains on the head; 6. Wipe off the alcohol remaining on the heating part of the recorder head using dry cotton swabs; 7. Wait at least 20 minutes till alcohol on the heating part of the recorder head volatilizes completely, and then install recorder paper and close the recorder door. 10-39
Figure 10-59 Heating part of recorder head and roller See the figure above to find the heating part of the recorder head and the roller when the recorder door is opened.
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11 Troubleshooting Your Analyzer

11.1 Introduction
The BC-2800Vet continuously monitors the status of the system and displays pertinent information in the lower left corner of the Count screen (the Error Message area). If a problem is detected, the Error Message area displays the corresponding error message. This chapter contains information that is helpful in locating and correcting problems that may occur during operation of your analyzer.
This chapter is not a complete service manual and is limited to problems that are readily diagnosed and/or corrected by the user of the analyzer. If the recommended solution fails to solve the problem, contact Mindray or your local distributor. Running samples in presence of error messages may lead to misleading results. If any error message occurs during sample analysis, remove the error first and then re-run the sample.
Unless otherwise instructed, always turn off the power before trying to fix the error.
11-1
Troubleshooting Your Analyzer
11.2 Errors without available error messages

Error The analyzer cannot be turned on. 1. 2. 3. Possible cause(s) power cord is broken or not well connected; The fuse is broken; The power outlet has no electricity. Liquid drips Damaged pump hose or blocked filter. 1. Turn off the power and wipe the analyzer dry; 2. Call Mindray customer service department or your local distributor for assistance. Recorder does not work. 1. Recorder paper is jammed; 2. Something is wrong with the circuit. 1. Remove the jammed paper; 2. If the problem remains, turn off the analyzer and turn it on again in 10 seconds. Recommend action 1. check the power cord connection; 2. Check the fuse; 3. Check the electrical outlet.
from analyzer inside.
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Troubleshooting Your Analyzer
11.3 Errors indicated by error messages

The analyzer can provide error messages. See the tables below for the error messages and their probable causes and recommended action. If the problem still remains after you have tried the recommended solutions, contact Mindray customer service department or your local distributor. Error Message Ambient temp. abnormal Possible Cause(s) Abnormal ambient temperature or temperature transducer error. Recommended Action 1. Enter the Service Status screen to check the ambient temperature; 2. If the actual ambient exceeds 15 to 30, adjust the temperature. Otherwise, the analysis results may be unreliable; 3. If the actual temperature is within the pre-defined range and the problem remains, contact Mindray customer service department or your distributor. Blank abnormal 1. 2. Contaminated diluent, diluent lines or bath; Expired diluent. 1. Check if the diluent is contaminated or expired; 2. Enter the Count screen and press the aspirate key to do the startup procedure; 3. If the problem remains, enter the Service Maintenance screen and do the probe cleanser cleaning procedure as instructed in Chapter 10.3.6. When the procedure is finished, return to the Count screen and do the background check again; 4. If the problem remains, contact Mindray customer service department or your local distributor.
HGB error
HGB blank voltage within 0 V to 3.2 V or 4.9 V to 5 V.
1. Do the Probe Cleanser Cleaning procedure as instructed in Chapter 10.3.6.; 2. If the problem remains, adjust the HGB gain as instructed by Chapter
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Troubleshooting Your Analyzer 5.3.4 to set the voltage within 3.4 to 4.8V, preferably 4.5V; 3. If the problem remains, shut down your analyzer and contact Mindray customer service department or you local distributor. HGB adjust HGB blank voltage within 3.2 V to 3.4 V or 4.8 V to 4.9 V. 1. Do the probe cleanser cleaning procedure as instructed in Chapter 10.3.6.; 2. If the problem remains, adjust the HGB gain as instructed by Chapter 5.3.4 to set the voltage within 3.4 to 4.8V, preferably 4.5V; 3. If the problem remains, shut down your analyzer and contact Mindray customer service department or you local distributor. WBC clog 1. Clogged WBC aperture; 2. Inappropriate WBC count time setting; 3. Solenoid valve error. 1. Enter the Service Maintenance screen. Zap and flush the aperture as instructed by Chapter 10.3.4 and 10.3.5; 2. Enter the Setup Settings Count screen and record the WBC count time. Then enter the Service Self-test screen and test the actual WBC count time as instructed by Chapter 10.5.1.; 3. If the difference between the reference WBC count time and the actual WBC count time is less than 2 seconds, the error has been removed; 4. If not, enter the Service Maintenance screen and do the probe cleanser cleaning procedure as instructed by Chapter 10.3.6; 5. Enter the Setup Settings Count screen and record the WBC count time. Then enter the Service Self Test screen and test the actual WBC count time as instructed by Chapter 10.5.1; 11-4
Troubleshooting Your Analyzer 6. If the difference between the reference WBC count time and the actual WBC count time is less than 2 seconds, the error has been removed; 7. If the difference is still greater than 2 seconds but consistent, enter the Setup Settings Count and reset the WBC count time. Then enter the Service Self-test screen and test the actual WBC count time as instructed by Chapter 10.5.1 to confirm the difference is less than 2 seconds; 8. If the problem remains, contact Mindray customer service department or your local distributor. WBC bubbles 1. Diluent or rinse running out; 2. Loose tube connections; 3. Inappropriate WBC count time setting. 1. Check if the diluent or rinse has run out. If so, change a new container of diluent or rinse as instructed in Chapter 4.4.1; 2. Check the connection of the diluent and rinse pickup tube. If necessary, reconnect and tighten them as instructed by Chapter 4.4.1; 3. If the problem remains, adjust the WBC count time as instructed by Chapter 5.3.5; 4. If the problem remains, contact Mindray customer service department or your local distributor. RBC clog 1. Clogged aperture; 2. Inappropriate RBC count time setting; 3. Solenoid valve error. 1. Enter the Service Maintenance screen. Zap and flush the aperture as instructed by Chapter 10.3.4. and 10.3.5.; 2. Enter the Setup Settings Count screen and record the RBC count time. Then enter the Service Self-test screen and test the actual RBC count time as instructed by Chapter 10.5.1.; 3. If the difference between the reference 11-5
Troubleshooting Your Analyzer RBC count time and the actual RBC count time is less than 2 seconds, the error has been removed; 4. If not, enter the Service Maintenance screen and do the probe cleanser cleaning procedure as instructed by Chapter 10.3.6.; 5. Enter the Setup Settings Count screen and record the RBC count time. Then enter the Service Self-test screen and test the actual RBC count time as instructed by Chapter 10.5.1.; 6. If the difference between the reference RBC count time and the actual RBC count time is less than 2 seconds, the error has been removed; 7. If the difference is still greater than 2 seconds but consistent, enter the Setup Settings Count and reset the RBC count time. Then enter the Service Self-test screen and test the actual RBC count time as instructed by Chapter 10.5.1 to confirm the difference is less than 2 seconds; 8. If the problem remains, contact Mindray customer service department or your local distributor. RBC bubbles 1. Diluent or rinse running out; 2. Loose tube connections; 3. Inappropriate RBC count time setting. 1. Check if the diluent or rinse has run out. If so, change a new container of diluent or rinse as instructed in Chapter 4.4.1; 2. Check the connection of the diluent and rinse pickup tube. If necessary, reconnect and tighten them as instructed by Chapter 4.4.1; 3. If the problem remains, adjust the RBC count time as instructed by Chapter 5.3.5; 4. If the problem remains, contact 11-6
Troubleshooting Your Analyzer Mindray customer service department or your local distributor. Trans error 1. Communication cable not well connected; 2. Inappropriate communication settings. 1. Check if the communication cable is well connected; 2. Check the communication settings as instructed by Chapter 5.3.2 and make sure they are the same with the host. Barcode error 1. Poor connection between the scanner and the analyzer; 2. Invalid bar-code. 1. Check if the analyzer is well connected to the analyzer; 2. Check if the bar-code is valid; 3. If the problem remains, contact Mindray customer service department or your local distributor. Barcode error com Poor connection between the scanner and the analyzer. 1. Check if the analyzer is well connected to the analyzer; 2. If the problem remains, contact Mindray customer service department or your local distributor. Printer paper out of Printer paper running out or not properly installed. 1. Check if there is printer paper; 2. Check if the printer paper is well installed. Printer offline Recorder error com Poor connection between the printer and the analyzer. 1. Poor connection between the recorder and the analyzer; 2. Damaged recorder. Check if the printer is well connected to the analyzer. 1. Print again; 2. If the problem remains, shut down the analyzer and restart it again, and then print again; 3. If the problem remains, shut down the analyzer Recorder out of paper Recorder paper running out or not properly installed. and contact Mindray customer service department. 1. Check if the recorder paper has run out. If so, install the paper as instructed by Chapter 4.4.2; 2. Check if the recorder paper is properly installed. If not, re-install the paper as instructed by Chapter 4.4.2; 3. If the problem remains, contact Mindray customer service department or your local distributor. 11-7
Troubleshooting Your Analyzer Recorder too hot Recorder head too hot. 1. Stop using recorder and cool it for 10 minutes and then print again; 2. If the problem remains, shut down the analyzer and restart it again, and then print again; 3. If the problem remains, contact Mindray customer service department. Lyse out Insufficient lyse or wrong lyse volume setting. 1. Check if there is sufficient lyse left. If so, access Setup Settings Reagents and adjust the remaining lyse volume as instructed by Chapter 5.3.1; 2. If not, change a new container of lyse as instructed by Chapter 4.4.1. Diluent expired Expired diluent or wrong expiration setting 1. Check if the diluent has expired. If so, change a new container of diluent as instructed by Chapter 4.4.1; 2. If not, reset the expiration date as instructed in Chapter 5.3.1. Rinse expired Expired rinse or wrong expiration setting 1. Check if the rinse has expired. If so, change a new container of rinse as instructed by Chapter 4.4.1; 2. If not, reset the expiration date as instructed in Chapter 5.3.1. Lyse expired Expired lyse or wrong expiration setting 1. Check if the lyse has expired. If so, change a new container of lyse as instructed by Chapter 4.4.1; 2. If not, reset the expiration date as instructed in Chapter 5.3.1. Vacuum error filter The air inside the vacuum chamber is not extracted within the given time. 1. Enter Service Selftest Tubingto test the filter as instructed in Chapter 10.5.1; 2. If the test result is normal, the error will be removed; 3. If the problem remains, replace the filter as instructed by Chapter 10.11; 4. If the problem still remains after a new filter has been installed, contact Mindray customer service department
11-8
Troubleshooting Your Analyzer or your local distributor. Real-time clock error 1. Someone tempered with the on-board battery off the board; 2. Something is wrong with the on-board battery (poor contact, dead battery, etc.); 3. Damaged real-clock chip. 1. Enter Setup Settings Date & Time screen and reset the time as instructed by Chapter 5.3.3; 2. Restart the analyzer after the adjustment and the time should be correct; 3. If the problem remains, contact Mindray customer service department or your local distributor. Syringe error motor 1. Poor contact of the motor; 2. Damaged motor; 3. Poor connection between the power drive board and the CPU board; 4. Malfunctioning photocoupler. Rotation Error Motor 1. Jammed sample probe; 2. Poor contact of the signal line; 3. Damaged motor; 4. Poor connection between the drive board and the CUP board; 5. Malfunctioning photo coupler. Elevator error motor 1. Jammed sample probe; 2. Poor contact of the signal line; 3. Damaged motor; 4. Poor connection between the drive board and the CUP board; 5. Malfunctioning photo coupler. 1. Enter Service Self-test Machine to test the syringe motor as instructed by Chapter 10.5.2.; 2. If the test result is normal, the error will be removed; 3. If the problem remains, contact Mindray customer service department or your local distributor. 1. Open the front door and check if the sample probe is jammed; 2. Enter Service Self Test Machinescreen to test the rotation motor as instructed by Chapter 10.5.2.. If the test result is normal, the error will be removed; 3. If the problem remains, contact Mindray customer service department or your local distributor. 1. Open the front door and check if the sample probe is jammed; 2. Enter the Service System Test screen and check the motor as instructed in Chapter 10.5.2. 3. The error will be removed if the test result is normal; 4. If the problem remains, contact Mindray customer service department or your local distributor.
11-9
Troubleshooting Your Analyzer A/D error Something is wrong with the A/D part of the CPU board.
1. Enter the Service Self-test Circuit screen to test the A/D interrupt as instructed by Chapter 10.5.4.; 2. If the test result is normal, the error will be removed; 3. If the problem remains, shut down the analyzer and contact Mindray customer service department or your local distributor.
Vacuum error
The vacuum degree does not reach the expected value within the given time.
1. Check whether the external tubing is pressed; 2. If not, enter Service Self-test Tubing to check the vacuum as instructed by Chapter 10.5.1..; 3. If the test result is normal, the error will be removed; 4. If the problem remains, contact Mindray customer service department or your local distributor.
Pressure error
The pressure inside the pressure chamber does not reach the expected value within the given time
1. Check whether the external tubing is pressed; 2. If not, enter Service Self-test Tubingscreen to check the pressure as instructed by Chapter 10.5.1..; 3. If the test result is normal, the error will be removed. 4. If the problem remains, contact Mindray customer service department or your local distributor.
Diluent empty
Insufficient diluent or wrong diluent volume setting.
1. Check if there is sufficient diluent left. If so, access Setup Settings Reagents and adjust the remaining diluent volume as instructed by Chapter 5.3.1; 2. If not, change a new container of diluent as instructed by Chapter 4.4.1.
Rinse empty
Insufficient rinse or wrong rinse volume setting. 11-10
1. Check if there is sufficient rinse left. If so, access Setup Settings
Troubleshooting Your Analyzer Reagents and adjust the remaining diluent volume as instructed by Chapter 5.3.1; 2. If not, change a new container of rinse as instructed by Chapter 4.4.1. Waste full The waste container is full. Empty the waste container and reset usable volume of the waste container as instructed by Chapter 5.3.1. File error Something is wrong with the analyzers file system. Dynamic memory error Analog error power Something is wrong with the analyzers memory. Something is wrong with the power board. Turn off the power and contact Mindray customer service department or your local distributor. Turn off the power and contact Mindray customer service department or your local distributor. Shut down the analyzer and contact Mindraycustomer service department or your local distributor.
11-11
12 Appendices
A
A
analyzer Intended use, 2-2 name, 2-1 aperture flush, 10-7 zap, 10-6 aperture size, 12-4 asperate key, 2-7 aspiration, 3-2 CV definition, 3-10 formula, 8-11 clean, 10-15 drain, 10-16 baud rate, 5-8 Blank Photocurrent, 3-6 bubbles RBC, 11-7 WBC, 11-5
Index
Impedance Principle, 3-1 count principle, 3-1 procedure, 3-5 screen, 2-11 customize date & time, 5-11 gain, 5-13 other, 5-21 Print & comm., 5-7 reagent, 5-5 reference range, 5-19
B
bath
D
DB9 connector, 12-16 diluent connection, 4-5 definition, 2-15 prime, 10-3 dilution, 3-3 dimensions, 3-5 drain tubing, 10-18
C
calibration auto, 9-4 calibrators, 2-16 manual, 9-10 preparations, 9-3 procedures, 9-3 purpose, 9-1 cleanser use, 10-11 clog RBC, 11-6 WBC, 11-4 control, 2-16 A-1
E
environment, 4-3 error A/D error, 11-10 ambient temp. abnormal, 11-3 barcode com error, 11-7 barcode error, 11-7 blank abnormal, 11-4 diluent empty, 11-11 diluent expired, 11-9
Appendices dynamic memory error, 11-12 elevator motor error, 11-10 filter error, 11-11 HGB adjust, 11-4 HGB error, 11-4 lyse expired, 11-9 lyse out, 11-8 press bar up, 11-8 pressure error, 11-11 printer offline, 11-8 printer out of paper, 11-8 real-time clock error, 11-9 recorder com error, 11-8 recorder out of paper, 11-8 recorder too hot, 11-8 rinse empty, 11-11 rinse expired, 11-9 rotation motor error, 11-10 syringe motor error, 11-9 trans error, 11-7 vacuum error, 11-10 vacuum filter error, 11-9 waste full, 11-11 E-Z cleanser definition, 2-15 lyse connection, 4-7 definition, 2-15 set HGB gain, 5-14 set RBC gain, 5-13 Gran# definition, 3-7 formula, 3-7 Gran% definition, 3-6 formula, 3-6 handshake, 5-9 Lyse test, 10-13 HGB carryover, B-3 definition, 3-5 linearity range, B-3 operating range, B-3 humidity, B-5
I
ID, 6-10 installation requirement, 4-2
L
LCD, 2-7 leukocyte granulocyte, 2-2 lymphocyte, 2-2 monocyte, 2-2 Lymph# definition, 3-7 formula, 3-7 Lymph% definition, 3-6 formula, 3-6
G
gain
M
maintenance system, 10-3 MCH definition, 3-10 formula, 3-10 MCHC definition, 3-10 formula, 3-10 MCV definition, 3-9 linearity range, B-3 A-2
H
HCT definition, 3-10 formula, 3-10
Appendices operating range, B-3 Mon# definition, 3-7 formula, 3-7 Mon% definition, 3-6 formula, 3-6 MPV definition, 3-10 format, 5-7 probe cleanser use, 10-8 probe cleaser definition, 2-15 probe wipe clean, 10-19 replace, 10-36 programming, D-4
N
NRBC, 3-6
Q
QC edit settings, 8-2 L-J graph, 8-10 run, 8-6 table, 8-12
O
optical sensors, 3-4
P
parameter description, 2-3 password, 5-2 PCT definition, 3-11 formula, 3-11 PDW definition, 3-10 performance specifications linearity range, B-3 operating range, B-3 PLT definition, 3-10 linearity range, B-3 operating range, B-3 power fuse, 4-2 voltage, 4-2 predilute mode sample collection and handling, 6-9 prediluted mode analyze, 6-17 prepare to ship, 10-21 printer connection, 4-10 A-3
R
RBC definition, 3-9 linearity range, 12-5 measurement, 3-9 operating range, B-3 RDW, 3-10 reagent connection, 4-5 recorder format, 5-8 reference range, 5-19 rinse connection, 4-6 definition, 2-15 prime, 10-5 RS-232 serial port, D-2
S
sample analysis, 6-11 review, 7-1 sample probe adjust, 10-32
Appendices sample probe localizer, 10-32 shutdown, 6-24 specification, B-3 system self-test, 10-24 status, 10-23
V
valve test, 10-26
T
table sample, 7-4 searched, 7-11 throughput, B-3 transmission at QC table screen, 8-13 at review screen, 7-18 data format, D-4 troubleshooting, 11-39 unpacking, 4-4
W
WBC definition, 3-6 formula, 3-6 linearity range, B-3 operating range, B-3 weight, B-6
Z
zap aperture, 10-6
A-4
B
B.1
Specifications
Reagents
V-28D DILUENT V-28R RINSE V-28CFL LYSE V-28E E-Z CLEANSER V-28P PROBE CLEANSER Specified by Mindray Specified by Mindray
Diluent Rinse Lyse E-Z Cleanser Enzyme cleanser Probe Cleanser Calibrator Control
B.2
Parameters
Table B-1 Directly measured parameters and histograms Abbreviation WBC RBC HGB PLT WBC Histogram RBC Histogram PLT Histogram Table B-2 Parameters derived from histograms
9
Parameter White Blood Cell or leukocyte Red Blood Cell or erythrocyte Hemoglobin Concentration Platelet WBC histogram RBC histogram PLT histogram
Default 10 /L 1012/L g/L 109/L / / /
Parameter Lymphocyte percentage Monocyte percentage Granulocyte percentage Mean Corpuscular Volume Red Blood Cell Distribution Width Mean Platelet Volume Platelet Distribution Width
Abbreviation Lymph% Mon% Gran% MCV RDW MPV PDW Table B-3 Calculated parameters % % % fL % fL /
Default
Parameter Lymphocyte Monocyte Granulocyte B-1
Abbreviation Lymph# Mon# Gran#

9
Default 10 /L 109/L 109/L
Appendices Hematocrit Mean Cell Hemoglobin Mean Cell Hemoglobin Concentration Mean Platelet Volume HCT MCH MCHC PCT Table B-4 Parameters and animals
Parameter Dog Cat Horse Rat Mouse Rabbit Monkey Cow Pig Buffalo Sheep Camel Goat Userdefined
% pg g/L %
WBC Lymph# Mon# Gran# Lymph% Mon% Gran% RBC HGB MCV MCH MCHC RDW HCT PLT MPV PDW PCT WBC Histogram RBC Histogram PLT Histogram
B.3
Sampling Features
B.3.1 Sample volumes required for each analysis

Whole Blood Mode (vein blood) Prediluted Mode (capillary blood) 13 L 20 L
B-2
Appendices
B.3.2 Aperture size

Diameter Aperture 80 m Length 70 m
B.3.3 Throughput
Less than 150 seconds/analysis
B.4
Performance specifications
B.4.1 Operating range

Parameter WBC (10 /L) RBC (10 /L) HGB (g/L) MCV (fL) PLT (10 /L)
9 12 9
Operating range 0.0 to 499.9 0.00 to 29.99 0.0 to 350.0 0.0 to 250.0 0 to 3999
B.4.2 Normal background

Parameter WBC RBC HGB HCT PLT Background result 0.3 109 / L 0.03 1012/ L 1g/L 0.5 % 10 109 / L
B.4.3 Linearity range

Parameter WBC (10 /L) RBC (10 /L) HGB (g/L) PLT (10 /L)
9 12 9
Linearity range 0.4 to 99.9 0.2 to 16.99 3 to 250 10 to 2999
B.4.4 Carryover
Parameter WBC Carryover 0.5 %
B-3
Appendices RBC HGB PLT 0.5 % 0.5 % 1%
B.4.5 Reproducibility (with normal-level control)

Parameter WBC RBC HGB MCV PLT Condition 7.5 to 15.0 109 / L 3.50 to 6.00 1012 / L 120 to 180 g/L 40.0 to 110.0 fL 200 to 500 109 / L (MCV>60.0) ReproducibilityCV% 3.0 2.0 2.0 2.0 6.0
B.5
Input/Output Device
Use the specified devices only.
B.5.1 Keypad
18-key keypad.
B.5.2 Keyboard
PS/2 keyboard.
B.5.3 Bar-code scanner (optional) B.5.4 Display

Color LCD, 7.8 640480
B.5.5 Recorder
Built-in thermal recorder that supports two printing formats and auto printing
B.5.6 Recorder paper

Width: 50 0.7 mm
+0
B-4
Appendices
To ensure the print quality and operating life of the recorder, it is recommended that you purchase the recorder paper from the analyzer manufacturer.
B.5.7 Printeroptional
EPSON LX-300+.
B.5.8 Interfaces
A keyboard interface. Two RS-232 interfaces (maximum transmission distance 12 meters); A parallel port(for printer or floppy disk drive); A power supply for the floppy disk driveonly to be used with the power cable supplied by Mindray. IDE hard disk interface.
B.6
Power supply
Voltage: 100 to 240 VAC; Frequency: 50/60 Hz; Input power:180 VA; Fuse: 250 V T4A
Use the fuse of the specified type and rating.
B.7
EMC Description
The product is subject to the EMC test as required by EN61326:1997+A1:1998+A2: 2001+A3 : 2003; EMS is compliance with experiment environment; EMC is compliance with Class A.
B-5
Appendices
B.8
Sound
Maximal sound: 65 dB
B.9
Operating environment
Operating temperature:15 to 30 ; Relative humidity: 30 % to 85 %; Atmospheric pressure: 60 kPa to 106 kPa.
B.10 Storage environment

Ambient temperature: 10 to 40 Relative humidity: 10 % to 93 % Atmospheric pressure: 50 kPa to 106kPa
B.11 Dimensions
Depth 38.6 cm Width 32.2 cm Height 43.7 cm
B.12 Weight
Less than 23 kg
B.13 Contraindications
None.
B-6
C
C.1
Precautions, Limitations and Hazards

Introduction
You will find the following symbols in this manual. When you see Then Read the statement below the symbol. The statement is alerting you to an operating hazard that can cause personnel injury. Read the statement below the symbol. The statement is alerting you to a possibility of analyzer damage or unreliable analysis results. Read the statement below the symbol. The statement is alerting you to information that requires your attention. Read the statement below the symbol. The statement is alerting you to a potentially biohazardous condition.
C.1.1 Installation Requirements

All the space, power and environmental requirements listed in Chapter 4 and Appendix B must be met. Establishing and maintaining proper grounding cannot be overemphasized.
C.1.2 Limitations
Whenever the results are outside the normal limits, it is recommended that the laboratory following whatever written protocol is in place for validating results. If an error occurs, the analyzer displays the corresponding error message In case of errors related to the fluidic system (such as clogging or bubbles), it is recommended that you re-run the sample after removing the error. If the PLT value is less than 100 109 / L, it is recommended the result be verified by a microscope.
C.1.3 Maintenance
The maintenance instructions in Chapter 10 describe corrective and preventive procedures that must be followed to ensure proper operation and performance of your analyzer.
C-1
Appendices
C.2
Warnings
It is important for the hospital or organization that employs this equipment to carry out a reasonable service/maintenance plan. Neglect of this may result in machine breakdown or injury to human health. Operate the analyzer under the condition specified in this manual; otherwise, the analyzer will not work normally and the analysis results will be unreliable, which would damage the analyzer components and cause personal injury. Make sure the analyzer is properly grounded. Before turning on the analyzer, make sure the input voltage meets the above requirements. When moving the analyzer, face the front of the analyzer and carry it from the bottom with hands! The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor. Do not place the analyzer in a flammable or explosive environment. Dispose of reagents, waste, samples, consumables, etc. according to government regulations. Avoid direct contact with animal blood samples. The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it. To avoid personal injury, keep your clothes, hair and hands away from such moving parts as the sample probe. Only install a fuse of the specified type and rating. Unless otherwise instructed, always turn off the power before trying to fix the error. Wear proper personal protective equipment (e.g. medical gloves, disposable wrist strap, etc.) against the static electricity during maintaining recorder. Make sure the power supply of the analyzer has been cut off before maintenance.
C-2
Appendices
C.3
Cautions
Installation by personnel not authorized or trained by Mindray may damage your analyzer. Do not install your analyzer without the presence of Mindray-authorized personnel. Do not place any container on the top of the analyzer. Do not re-use disposable products. Do not perform any maintenance procedures that are not described in this chapter. Performing unauthorized maintenance procedures can damage your analyzer. In case of problems not specified in this manual, contact Mindray customer service department or your local distributor for assistance. Only Mindray-supplied parts can be used for maintenance. For any questions, contact Mindray customer service department or your local distributor. Use only specified recorder paper. Otherwise, it may cause damage to the recorder head, or the recorder may be unable to print, or poor print quality may result. Never pull the recorder paper with force when a recording is in process. Otherwise, it may cause damage to the recorder. Do not leave the recorder door open unless you install paper or remove trouble. Improper installation of recorder paper may jam the paper and/or result in blank printout. If the recorder finishes a print action, wait at least 20 minutes until the recorder head is cooled completely and then maintain the recorder. Do not add unnecessary force to the recorder head. Wipe off the alcohol remaining on the recorder head in time after cleaning the recorder head. Make sure the alcohol volatilizes completely before using the recorder to print.
C-3
Appendices
C.4
Notes
This equipment must be operated by skilled/trained medical professionals. Operate your analyzer strictly as instructed in this manual. This analyzer adopts a fixed decimal point. You can enter the digits without bothering to look for the [.] on the external keyboard. The purpose of this analyzer is to identify the normal animal patient, with all normal system-generated parameters, and to flag or identify patient results that require additional studies. Before connecting the power cord, make sure the power switch at the back of the analyzer is placed in the off (O) position. If the ambient temperature is outside the specified operating range, the analyzer will alarm you for abnormal ambient temperature and the analysis results may be unreliable. See Chapter 11 Troubleshooting Your Analyzer for solutions. Retain the shipping carton and all the packing materials, as they can be used for packaging if analyzer must be reshipped. Use the specified reagents. Never use expired reagents. To prevent contamination, tighten the container caps when the installation is finished. Use the printer and scanner of the specified model. Store and use the reagents as directed by instructions for use of the reagents. When you have changed the diluent, rinse or lyse, run a background to see if the results meet the requirement. Pay attention to the expiration dates and open-container stability days of all the reagents. Never use expired reagents. After installing new reagents, let them stand for a while before using them. The recorder paper is treated on one side for printing. To determine which side is the printing side, gently scratch both sides with a fingernail and the one with visible nail trace left is the printing side. For the external printer, you can only select Vertical. Once you have selected another animal, the analysis result and sample information regarding the current sample will be cleared, but the next C-4
Appendices sample ID will remain unchanged. Whole blood samples to be analyzed for WBC differential or PLT count should be stored at room temperature and run within 4 hours of collection. If you do not need the PLT, MCV and WBC differential results, you can store the samples in a refrigerator (2 to 8) for 24 hours. You need to warm the refrigerated samples at room temperature for at least 30 minutes before running them. Mix any sample that has been prepared for a while before running it. Keep dust from the prepared diluent. After mixing the capillary sample with the diluent, wait 5 minutes before running the sample. Run the prediluted samples within 30 minutes after the mixing. Evaluate predilute stability based on your laboratorys sample population and sample collection techniques or methods. If you intend to do the background check instead of a patient sample, enter 0 into the ID box. Keep the sample probe tip away from the tube bottom; otherwise the aspiration volume may be inaccurate. When the aspiration is done, remove the sample tube only when the sample probe is out of the tube. If the analyzer detects WBC/RBC clogging or bubbles during the analysis, the corresponding error messages will be displayed in the error message area and the results of all the related parameters will be invalidated. See Chapter 11 Troubleshooting Your Analyzer for solutions. The result of the background check will not be flagged. Select proper reference range as instructed in Chapter 5.3.6 before running the samples. Otherwise, the obtained results may be erroneously flagged. To ensure stable analyzer performance and accurate analysis results, perform the Shutdown procedure to shut down the analyzer after it has been running continuously for 24 hours. Shut down the analyzer strictly as instructed below. Fur and skin debris may block the aperture. Keep the sample clean before using the analyzer to analyze it. Samples of the dogs infected with Filaria Larva in Blood may clog the aperture. For every search, the analyzer can display a maximum of 500 matches. The matches will be deleted if you have run another sample (including background check), or deleted a sample result, or restarted the analyzer
C-5
Appendices after the search. Use the specified controls. Using controls other than the specified will lead to misleading results. Refer to the instructions of use of the controls for how to store and use the controls. Refer to the instructions of use of the control for information on the lot number, expiration date, open-vial stability days, expected results and limits. The entered expiration date should be either the expiration date printed on the labeling or the open-vial expiration date. It is earlier. The open-vial expiration date is calculated as follows: the date that vial is opened + the open-vial stability days. At the QC Edit screen, if you want to correct an erroneous entry, MODIFY the wrong digit. All of the measured parameters must be calibrated before readings of this analyzer can be used as valid analysis results. Use the Mindray- specified calibrator. Using calibrator other than the specified will lead to misleading results. Refer to the instructions of use of the calibrator for how to store and use the calibrator. In the prediluted Calibration screen, you cannot dispense diluent from the analyzer. It is recommended that you prepare at least 7 cups of diuent before starting calibrating the analyzer in the prediluted mode. Keep dust from the prepared diluent. Do the Drain Tubing procedure before relocating the analyzer. This chapter is not a complete service manual and is limited to problems that are readily diagnosed and/or corrected by the user of the analyzer. If the recommended solution fails to solve the problem, contact Mindray or your local distributor. Remove the protective paper between the recorder head and the roller inside the recorder before installing recorder paper. Maintain the recorder strictly as instructed below. To ensure the print quality and operating life of the recorder, it is recommended that you purchase the recorder paper from the analyzer manufacturer.
C-6
Appendices
C.5
Biohazard
C-7
D
D.1
Communication
Introduction
The BC-2800Vet can transmit the sample data and QC data to an external computer (a host) through its RS-232 serial port. The transmission can be conducted either automatically or through the command of the operator after the completion of the sample analysis. This section gives detailed discussion about the setup of transmission parameter, RS-232 serial port and the data transmission format, therefore, providing detailed information for the software engineers to program and for the user to conveniently perform transmission.
D-1
Appendices
D.2
Connection
The BC-2800Vet can be connected with an external computer through a DB9 connector. The pins of the DB9 connector are shown in Figure D-1.
Figure D-1 DB9 connecter Pin description: DCD: Carrier Detect RXD: Receive Data TXD: Transmit Data DTR: Data Terminal Ready GND: Signal Ground DSR: Data Set Ready RTS: Request to Send CTS: Clear to Send RI: Ring Indicator The BC-2800Vet communicates with a host through serial port 2, using Pin2, Pin 3 and Pin 5. The maximum transmission distance is 12 meters.
D-2
Appendices
D.3
Transmission Data Format
D.3.1 Description Symbols

[ENQ] [STX] [EOT] [EOF] [ETX] [ACK] [NACK] "A" "B" "C" "#" "*" 0x05 0x02 0x04 0x1A 0x03 0x06 0x15 0x41 0x42 0x43 0x30-0x39 0x2A
If the Lot No., Month, Day, Year are empty in QC Edit menu, the * (2A Hex) will be transmitted to the host. For all the data formats, if the data are marked *, then * (2A Hex) will be transmitted to the host. L1 Region to L8 Region are LI to L8 of eight histogram discriminators as shown in Figure D-2.
Figure D-2 L1 to L8
D-3
Appendices
Programming
If the Handshake is off, the BC-2800Vet will transmit the body of the text without acknowledging the presence of an external computer. If the Handshake is on, the BC-2800Vet will communicate with the external computer in following procedures:
1. The BC-2800Vet sends an ENQ (05 Hex), then waits up to 4 seconds for the external computer to respond. If the external computer does not respond, then one more ENQ (05 Hex) is tried. If it fails again, the analyzer aborts the transmission and reports a transmission error; 2. The external computer must respond by sending an ACK (06 Hex). If any other response is received, another ENQ (05 Hex) will be sent by the analyzer (a maximum of two ENQ [05 Hex] will be sent); 3. The analyzer then sends: Body of text EOT (04 Hex) ETX (03 Hex) 4. Disconnection. The BC-2800Vet sends an ETX (03 Hex), then waits 4 seconds for the external computer to respond. If no response is received, one more ETX ( 03 Hex) is sent, the BC-2800Vet waits 4 seconds before giving up and gives alarm of communication error. If the external compute responds ACK, the transmission is done successfully. If the external computer responds NACK15 Hex, the analyzer repeat the transmission from step 3. If the received response from the computer is neither ACK06 Hex nor NACK15 Hex, the analyzer sends ETX ( 03 Hex) again.
D.3.2 Sample Data Format

Animal Type:
00 01 02 03 04 05 06
D-4
Dog Cat Horse Pig Cow Buffalo Goat
Appendices
07 08 09 11 12 13 14 15 16
Animal1 Animal2 Animal3 Rat Mouse Rabbit Camel Sheep Monkey
For Cat, Dog, Horse, Rat, Mouse, Rabbit, Monkey and Cow If handshake is enabled If handshake is disabled Body of the text start Text Identifier ID Sample Mode Month Day Year Hour Minutes WBC[10 /L] Lymph#[10 /L] Mon#[10 /L] Gran#[10 /L] Lymph%[%] Mon%[%] Gran%[%] RBC[10 /L] HGB[g/L] MCHC[g/L] MCV[fL] MCH [pg] RDW [%] HCT[%] PLT[10 /L] MPV[fL] PDW PCT[%] Reserved AnimalType
9 12 9 9 9 9
[ENQ] [STX] A ######## # ## ## #### ## ## ###.# ###.# ###.# ###.# ##.# ##.# ##.# ##.## ### #### ###.# ###.# ##.## ##.# #### ##.# ##.# .### ############## ## D-5
Appendices Reserved L1 Region L2 Region L3 Region L4 Region L5 Region L6 Region L7 Region L8 Region Reserved WBC Histo (256 channels) RBC Histo (256 channels) PLT Histo (256 channels) Body of the text end If handshake is enabled If handshake is disabled If handshake is enabled [EOT] [EOF] [ETX] ######## ### ### ### ### ### ### ### ### ################ ### ### ###
For pig, Buffalo, Camel, Sheep and User-defined If handshake is enabled If handshake is disabled Body of the text start Text Identifier ID Sample Mode Month Day Year Hour Minutes WBC[10 /L] Reserved Reserved RBC[10 /L] HGB[g/L] MCHC[g/L] MCV[fL] MCH [pg] RDW [%] HCT[%] PLT[10 /L] MPV[fL]
9 12 9
[ENQ] [STX] A ######## # ## ## #### ## ## ###.# ################ ##### ##.## ### #### ###.# ###.# ##.## ##.# #### ##.# D-6
Appendices PDW PCT[%] Reserved AnimalType Reserved L1 Region L2 Region L3 Region L4 Region L5 Region L6 Region L7 Region L8 Region Reserved WBC Histo (256 channels) RBC Histo (256 channels) PLT Histo (256 channels) Body of the text end If handshake is enabled If handshake is disabled If handshake is enabled For Goat If handshake is enabled If handshake is disabled Body of the text start Text Identifier ID Sample Mode Month Day Year Hour Minutes WBC[10 /L] Reserved Reserved RBC[10 /L] HGB[g/L] MCHC[g/L] MCV[fL] MCH [pg]
12 9
##.# .### ############## ## ######## ### ### ### ### ### ### ### ### ################ ### ### ### [EOT] [EOF] [ETX]
[ENQ] [STX] A ######## # ## ## #### ## ## ###.# ################ ##### ##.## ### #### ###.# ###.# D-7
Appendices RDW [%] HCT[%] Reserved Reserved Animal Type Reserved L1 Region L2 Region L3 Region L4 Region L5 Region L6 Region L7 Region L8 Region Reserved WBC Histo (256 channels) RBC Histo (256 channels) PLT Histo (256 channels) Body of the text end If handshake is enabled If handshake is disabled If handshake is enabled [EOT] [EOF] [ETX] ##.## ##.# ############# ############## ## ######## ### ### ### ### ### ### ### ### ################ ### ### ###
D.3.3 Standard L-J QC Data Format

If handshake is enabled If handshake is disabled Body of the text start Text Identifier File No. Lot No. Month Day Year WBC[10 /L] RBC[10 /L] HGB[g/L] PLT[10 /L] Reserved HCT[%] MCV[fL] MCH[pg]
9 12 9
[ENQ] [STX] B # ###### ## ## #### ###.# ##.## ### #### ############## ##.# ###.# ###.# D-8
Appendices MCHC[g/L] WBC Limit[10 /L] RBC Limit[10 /L] HGB Limit[g/L] PLT Limit[10 /L] Lymph# Limit[10 /L] Lymph% Limit[%] Gran# Limit[10 /L] Gran% Limit[%] HCT Limit[%] MCV Limit[fL] MCH Limit[pg] MCHC Limit[g/L] Body of the text end If handshake is enabled If handshake is disabled If handshake is enabled [EOT] [EOF] [ETX]
9 9 9 12 9
#### ###.# #.## ### #### ###.# ##.# ###.# ##.# ##.# ###.# ###.# ####
D.3.4 Run L-J QC Data Format

If handshake is enabled If handshake is disabled Body of the text start Text Identifier Month Day Year Hour Minutes WBC[10 /L] RBC[10 /L] HGB[g/L] PLT[10 /L] Reserved HCT[%] MCV[fL] MCH[pg] MCHC[g/L] Body of the text end If handshake is enabled If handshake is disabled If handshake is enabled [EOT] [EOF] [ETX]
9 12 9
[ENQ] [STX] C ## ## #### ## ## ###.# ##.## ### #### ############## ##.# ###.# ###.# ####
D-9
Appendices
D.4
Transmission
D.4.1 Defining Transmission Settings

The data format is fixed for the transmission so that every byte to be transmitted has 7 data bits and 1 stop bit. Enter the Setup Settings Print & comm. screen and edit the communication settings as instructed by Chapter 5.3.2.
D.4.2 Transmission at Count Screen

If the auto transmission function is on, once the analysis is done, the analyzer will automatically transmit the results to the external computer. If the auto transmission function is off, you can only transmit the results manually at the Review screen.
D.4.3 Transmission at the Review Screen

Select the results you want to transmit and transmit them to the external computer as instructed by Chapter 7.2.2. and Chapter 7.3.2.
D.4.4 Transmission at the QC Table Screen

Transmit the results as instructed by Chapter 8.4.2..
D-10
P/N: 2810-20-472021.8

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BC-2800Vet

Auto Hematology Analyzer

Intellectual Property Statement

are the trademarks, registered or otherwise, of Mindray in China and

Responsibility on the Manufacturer Party

This equipment must be operated by skilled/trained clinical professionals.

EC-Representative: Address: Tel: Fax:

Using This Manual

Using This Manual

1.2 Who Should Read This Manual

Using This Manual

1.3 How to Find Information

Your Analyzer Chapter 2 Understanding

Limitations and Hazards Appendix D Communication

Using This Manual

1.4 Conventions Used in This Manual

Using This Manual

1.5 Special Terms Used in This Manual

Using This Manual

DOCUMENTS. BIOLOGICAL RISK

Using This Manual SERIAL NUMBER

CONSULT INSTRUCTIONS FOR USE

CONCERNING DIRECTIVE DIRECTIVE

AUTHORISED REPRESENTATIVE IN THE EUROPEAN COMMUNITY IRRITATING SUBSTANCE

PRODUCT IS DISPOSED OF CORRECTLY, YOU WILL HELP PREVENT BRINGING

INFORMATION RETURNING PRODUCT,

RECYCLING CONSULT WHOM

PURCHASED THE PRODUCT.

Using This Manual

Figure 1-1 Back of the analyzer

Figure 1-2 Warning label

Using This Manual

Figure 1-3 Warning label

Using This Manual

Figure 1-4 warning label

Understanding Your Analyzer

Understanding Your Analyzer

2.2 Intended Use

WBC Histogram RBC Histogram PLT Histogram

Understanding Your Analyzer

Table 2-1 Parameters and animals

Understanding Your Analyzer

2.3 User Interface

Figure 2-1 Front view

1 ---- LCD 3 ---- Recorder 5 ---- Aspirate key

2 ---- Keypad 4 ---- Power indicator 6 ---- Sample probe

Understanding Your Analyzer

Figure 2-2 Back view

1 --- Power switch 3 --- Waste outlet(Red) 5 --- Rinse inlet(Blue)

2 --- Equipotentiality 4 --- Lyse inlet(Orange) 6 --- Diluent inlet(Green)

Understanding Your Analyzer

Figure 2-3 Left view

1 --- RS-232 port1 3 --- RS-232 port2 5 --- Keyboard interface

2 --- Parallel port 4 --- Power Interface of Floppy Disk Drive

Understanding Your Analyzer

2.3.2 Input Devices

Figure 2-4 Sample probe and aspirate key

Understanding Your Analyzer

Understanding Your Analyzer

2.3.4 Keyboard Interface

2.3.5 Serial Ports

2.3.6 Parallel Port