List of Project Topics

Molecular and Recombinant DNA Technology

1.

Study of genetic diversity and genetic relatedness of plant samples. Identification of Varietal purity in crops.

2.

3. Inheritance of transgene in GM Crops

4. 5.

Gene regulation and gene identification by using PCR. Gene cloning, expression and regulation in prokaryotic systems. Gene cloning and Expression, conformation by Blotting and PCR. Molecular characterization of proteins in prokaryotic and eukaryotic systems. DNA finger printing of plant sources by using different molecular markers. Construction of genetic map prokaryotic samples/and Fungi. of plant samples /

6.

7.

8.

9.

10. 11.

Restriction mapping on Prokaryotic system. Molecular characterisation of plant sources /prokaryotes /fungi by molecular RAPD/RFLP/AFLP/ISSR/ SSR/CAPS. markers Like

12.

Genetic and protein characterization of microbes (e.g. Pseudomonas sps, E.coli, etc.).

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13.

Molecular characterisation of microbes (leaching/recalcitrant).

industrially

useful

14.

Mutational and molecular characterisation of microbes resistant to antibiotics. Construction of genetic molecular Markers. map of phytoflora, using

15.

16.

Molecular characterisation of Phosphate solubilising organisms. Isolation and Molecular Characterisation of Bt Strains

17.

Enzyme Biotechnology
1.

Isolation and characterisation of Extremophiles:

i) ii)

Hyperthermophiles e.g: Proteases Thermophiles e.g: Taq polymerase, Glycosyl hydrolases, Chitinases, Xylanases, Lipases, esterases, Dehydrogenases

iii)

Psycrophiles e.g: proteases, dehydrogenases, cellulases, lipases Halophiles e.g- Proteases,dehydrogenases

amylases,

iv) v)

Alkaliphiles e.g- proteases,amylases,glucoamylases Phosphatase

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vi)

Acidophiles e.gamylases, proteases, cellulases, Glucose oxidases

glucoamylases,

2.

Production and purification of pharmaceutical grade enzymes like Streptokinase/serratia peptidase /Bromelian /Natokinase /ALP/ glucose oxidase/Heparinase etc.. Isolation, Purification and Characterisation of enzymes from Plant source enzyme like HRP/Peroxidases/ Papain/ Bromelian/ Amylase/Phenol Oxidase

3.

4.

Production and characterisation of industrially enzymes like Serine Protease/ amylase/cellulase/lipase/diastase /Keratinase/Phytase/Superoxide dismutase

useful Alpha

5.

Isolation and Purification of Biodegrading enzymes like ligninase/laccase/metaloperoxidase/pectinase/Azoreducta se/ Xylanase etc

6.

Strain improvement for the production of enzymes from prokaryotes and Fungi. Production and Purification of food Processing enzymes like Pullunase/Inulinase/Pectinase/Beta- galactosidase.

7.

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8.

Isolation and purification L-Asparaginase.

of

Anticancer

enzymes

9.

Isolation and Characterisation of Anticoagulant enzymes.

Fermentation and Downstream process/Industrial Biotechnology

1. Production and purification of Amino acids
2.

Production and purification SSF/SMF

of Organic Acids from

3.

Production, purification and characterisation of Enzymes SSF/SMF Standardisation of media for the production of Enzymes

4.

5. Production and Purification of Antibiotics 6. Optimisation for the /Antibiotics /amino acids
7.

Production

of

Enzymes

Isolation and purification of natural pigments from Prokaryotes / Fungi. Extraction and purification of toxins from Prokaryotes and Fungi.

8.

ENVIRONMENTAL BIOTECHNOLOGY

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1. Isolation, identification and molecular characterization of microbes from effluents of textile, leather, paper and other industries. 2. Comparative and mutation studies on biodegradation. 3. Biodegradation of thermal effluents.
4.

Biodegradation of PAH compounds.

5. Biodegradation of Hydrocarbons. 6. Biodegradation of azodyes. 7. Study on biodegradable enzymes like peroxidase, laccase, azo-reductase...etc. 8. Biodegradation of lignin compounds. 9. Bioleaching (studies on heavy metals). 10. Characterization of phytoflora near industries.

11. Isolation and characterization of microbes involved in degradation of pesticides and insecticides. 12. 13. 14. Effect of pesticides/insecticides on bio fertilizers. Xenobiotics degradation. Study on Biodegradation of e-waste.

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Plant Molecular Biology/Plant biotechnology

1. Detection of transgenic plants by Bio Assays and PCR. 2. Confirmation of transgenic plants by NPT2 and Gus assay. 3. Molecular characterisation of plants by Isozymes. 4. Tissue culture studies on medicinal plants with focus on secondary metabolites. 5. Micro propagation of plants/ornamental plants. endangered medicinal

6. Studies on Agro bacterium Mediated gene Transformation and confirmation by bio Assay and PCR. 7. Isolation and characterisation of plant pathogens. 8. Studies on Agro bacterium Mediated gene Transformation and confirmation by bio Assay and PCR. 9. Isolation and characterisation of plant pathogens.

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10. Isolation and characterisation of active compounds from plants. 11. Hormonal effects on callus induction and secondary metabolite . 12. Standardisation of callus regeneration

Phytochemical studies 1. Extraction (Soxlet) and analysis metabolites from medicinal plants of secondary

2. Extraction and analysis of antimicrobial compounds from plants 3. Isolation and characterisation of active compounds from plants 4. Extraction and analysis of active ingredients from citrus fruits. 5. Comparative studies on antimicrobial and antifungal compounds from plants.

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