SHOBHIT UNIVERSITY, MEERUT SCHOOL OF BIOTECHNOLOGY M.Tech. (Biotechnology): 1st year (I and II semester) M.

Tech (Integrated) 4th year (VII and VIII semesters) 1st year (I Semester)/4th year (VII Semester) Course Code BT-501 Course/ Title Biostatistics and Mathematical models L 3 3 3 3 0 T 2 0 0 0 0 P 0 4 4 4 2 Cr 5 5 5 5 1

BT-503/BT-403 Down Stream Processing BT-505/BT-405 Animal Biotechnology BT-507 BT-581 Protein Engineering Seminar

Credits=21 1st year (II Semester)/4th year (VIII Semester) Course Code BT-502 BT-504 BT-506 BT-508 BT-582 Course/ Title Biomedical Instrumentation Microbial and Fermentation Technology Biochemical Engineering Principles of Management and Entrepreneurship Development Seminar L 3 3 3 4 0 T 0 0 0 1 0 P 4 4 4 0 2 Cr 5 5 5 5 1

Credits=21

BIOSTATISTICS AND MATHEMATICAL MODELS BT-501 Cr L 5 3 T 2 P 0

Unit I: Fundamentals of Biostatistics: Review of basic notions of statistics. Measures of central tendency viz. mean, median, other quartiles, mode, measures of dispersion and variability viz. range, mean deviation, variants, standard deviation, coefficient of variation, measure of symmetry and kurtosis, covariance and correlation. Unit II: Probability and Expected values: Counting possible outcomes, permutation and combination, Probability of an event, adding and multiplying probability, total and conditional probability, Bayes theorem, Random event, mutually exclusive events, independent events, Expectation of a random even, Probability distribution, marginal and conditional distribution. Unit III: i. Theoretical Probability Distributions: (a) Discrete distributions viz. Binomial and Poisson distribution. Distinctive properties of these distributions. (b) Continous distributions viz. Normal, chi square, t, F distribution. Distinctive properties of these distributions. ii. Population and Samples: Variability of sample, population and sample; Confidence intervals: a way of precisely representing uncertainty. iii. Statistical Hypothetical Testing: One-sample, two-sample, paired-sample, multisample hypothesis. One and two tailed test. Analysis of variance viz. single-factor, two-factor, three-factor and higher order factorial analysis of variance. iv. Regression and Correlation: Simple linear regression, simple linear correlation, multiple regression and correlation, polynomial regression. v. Testing for goodness of fit: chi square goodness of fit, Kolmogorov-Smirnov goodness of fit. Unit IV: Applications of statistics in Biotechnology: Genetic linkage analysis, LOD score; gene expression prediction; protein function prediction; protein structure prediction. Unit V: Mathematical models in Bio and Chemo system: General linear model; Optimal prediction models; Genetical theory of Natural selection: Darwin, Lamarck and Mendels contribution; Population growth: Logistic equation/Verhulst-Pearl model; Ecological predator-prey model: Lotka-Volterra model.

REFERENCE BOOKS: 1. Zar, J.H. 2009. Biostatistical Analysis (5th eds.). Pearson Education Inc. 2. Miller, I.R., Freund, J.E. and Johnson, R. 1992. Probability and Statistics for Engineers (4th eds.), Prentice- Hall of India Pvt. Ltd. 3. Grafen, A. and Hails, R. 2008. Modern Statistics for the Life Sciences, Oxford University Press. 4. Montgomery, D. C. and Runger, G. C. 1994. Applied Statistics and Probability for Engineers (3rd eds.), John-Wiley and Sons. 5. Falconer, D. S., and Mackay, T. F. C. 2009. Introduction to Quantitative Genetics (1st eds.), Pearson Education Inc.

DOWN STREAM PROCESSING BT-503/BT-403 Cr L T P 5 3 0 4

Unit I: i. Cell lysis and flocculation: biomass removal and cell disruption, chemical lysis, enzymatic lysis, physical and mechanical means of disruption, flocculation. ii. Sedimentation: sedimentation principles, methods and coefficients, centrifuges, ultra centrifugation, sedimentation of low accelerations. Unit II: Membrane separation process: Filtration, filtration principles, ultrafiltration, electrophoresis, electrodialysis and isoelectric focusing, membrane for liquid-liquid extraction, reverse osmosis, separation of liquids, dialysis. Unit III: Chromatography: Classification, concepts of retention factor, capacity factor, partition coefficient, column efficiency; ion exchange chromatography, gas chromatography, gel filtration chromatography, HPLC, UFLC, affinity chromatography, adsorption , reverse phase chromatography etc. Unit IV: Distillation operations: Basic principles of the following distillation operations, batch, continuous, flash, steam, vacuum, molecular distillations, azeotropic and extractive distillation. Unit V: i. Extraction and Drying: extraction, extraction principles, drying, drying principles, dryer description of operation. ii. Precipitation: protein solubility, precipitate formation phenomena, methods of precipitation. iii. Crystallization: crystallization principles, batch crystallizers, process crystallization of proteins, lyophilisation. 1. 2. 3. 4. REFERENCE BOOKS: Treybal E. Robert. 1993. Mass-Transfer operations (3rd eds). McGraw-Hill International Edition, Singapore. Doran M. Paulines. 2003. Bioprocess engineering principles (8th eds). Academic press, New York. Warren, M.L., Julian, S.C. and Peter Harriott. 2001. Unit Operations of Chemical Engineering (6th eds). McGraw-Hill International Edition, New York. Bailey, J. E. and Ollis, D.F. 1986. Biochemical Engineering Fundamentals (2nd eds.). McGraw-Hill Inc.

Lab. Based on theory syllabus

ANIMAL BIOTECHNOLOGY BT-505/BT-405 Cr L T P 5 3 0 4

Unit I: Human genetics and human genome: Organization of human genome, repetitive DNA in human genome-Alu and SINE repeats, human genome mapping, application of human genome project in drug design, gene therapy, introduction to pharmacogenomics, pedigree analysis, inherited human diseases, genetic counseling. Unit II: Biotechnology in animal and human health care: Recombinant vaccines in disease prevention, subunit vaccines, DNA vaccines, synthetic peptide vaccine, plants as edible subunit vaccine, attenuated recombinant vaccines, vector recombinant vaccines, marker vaccines, vaccine delivery systems, DNA in disease diagnosis, DNA and RNA probes, detection of genetic diseases, Immunotherapy, diagnostic application of MAbs, monoclonal antibody therapy. Unit III: Reproductive Biotechnology: In vitro fertilization, embryo transfer technology, applications and limitations of ETT, Cryopreservation of embryo, embryo splitting, embryo sexing, embryo cloning, methods of animal cloning, nuclear transfer for cloning, cloning from embryonic cells, cloning from adult and fetal cells, ethical issues and risks associated with cloning. Unit IV: Recombinant protein therapeutics and stem cells: Recombinant proteins of clinical significance, alpha interferon, interleukin-2, colony stimulating factors, tissue plasminogen activator, erythropoietin, insulin, glucagons, expression systems for commercial production of recombinant proteins, stem cells in health care, primordial germ cells, embryonic stem cells, embryonal carcinoma cells, trophoblast stem cells, hemaetopoietic stem cells, mesenchymal stem cells, epidermal stem cells, adult stem cells vs. embryonic stem cells, migration of stem cells to injured tissues, therapeutic cloning of embryonic stem cells, human stem cells and biotechnology companies. Unit V: Biotechnology in livestock production: Selected traits and their breeding into livestock, diagnosis, elimination and breeding strategies of genetic diseases, hybridization based markers, PCR based markers, properties of molecular markers, transgenic breeding strategies.

REFERENCE BOOKS: 1. Singh, B.D. 2008. Biotechnology-Expending horizons (2ndeds.). Kalyani Publications. 2. Gupta, P.K. 2008. Biotechnology and Genomics (1st ed). Rastogi Publications. 3. Brown, T.A. 2008. Gene cloning and DNA analysis (5th eds). Blackwell Sciences Ltd. 4. Dubey, R.C. 2007. A textbook of biotechnology (4th eds.). S. Chand and Company Ltd. 5. Satyanarayana, U. 2008. Biotechnology. Uppala Author Publisher Interlink. 6. Balasubramanian, D., Bryce C.F.A., Dharmalingam, K., Green, J. and Kunthala Jayaraman. Concepts in Biotechnology.Revised edition.Universities press. 7. Srivastava, A.K., Singh, R.K. and Yadav, M.P. 2006. Animal Biotechnology. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi.

Lab. based on theory syllabus

PROTEIN ENGINEERING BT-507 Cr L T P 5 3 0 4

Unit I: Introduction to protein engineering: Amino acids, primary structure of proteins, secondary structure, tertiary structure, quaternary structure; protein folding pathways and thermodynamics of folding, denaturation, renaturation Unit II: Characterization of proteins: NMR spectroscopy, UV IR spectroscopy, X Ray crystallography, Mass spectrometry, Circular dichroism; Steps in purification and characterization of proteins Unit III: Structurefunction relation of proteins: Prokaryotic and eukaryotic DNA binding proteins, amylases, proteases, lipases, EGF, FBF, phosphatases, dephosphorylases, kinases, apoptosis. Unit IV: Production of recombinant proteins: Synthetic peptide synthesis, coupling, polymerization, conjugation etc., strategies of heterologous expression, methods for expressing recombinant proteins in yeast, site directed mutagenesis for specific protein function, concepts for design of a new protein Unit V: Applications of protein engineering: Protein engineering in drug discovery, industrial chemical synthesis, transgene plant research; examples of protein engineering, subtilisin, dihydrofolate reductase etc. 1. 2. 3. REFERENCE BOOKS: Paul R. Carey. 1996. Protein engineering and design Academic Press. Kohree, C. and. Bhandary, U. L. R. Protein engineering (Nucleic acids and Molecular biology) (Ist ed.). Springer. Cleland, J.L. and Gaik, C.S. Protein Engineering: Principles and Practice (1 st ed.). Wileyliss. Lab. based on theory syllabus

BIOMEDICAL INSTRUMENTATION BT-502 Cr L T P 5 3 0 4

Unit I: Bio-potential electrodes: Electrode electrolyte interface, half-cell potential, polarizations and non- polarizable electrode, calomel electrode, needle and wire electrode, microelectrode-metal micropipette. Unit II: Recording system: Low-Noise preamplifier, main amplifier and driver amplifier, inkjet recorder, thermal array recorder, photographic recorder, magnetic tape recorder, X-Y recorder, medical oscilloscope. Unit III: Bio-chemical measurement: pH, pO2, pCO2, pHCO3, electrophoresis, colorimeter, spectro photometer, flame photometer, auto analyzer. Unit IV: Non-electrical parameter measurements: Respiration, heart rate, temperature, pulse blood pressure, cardiac output, O2, CO2 measurements. Unit V: Blood flow and blood cell counting: Electromagnetic and ultrasonic blood flowmeter, indicator dilution method, thermo dilution method, manual and automatic counting of RBC, WBC and platelets. TEXT BOOK: 1. Leslie Cromwell. 1997. Biomedical Instrumentation and Measurement. Prentice Hall of India, New Delhi. 1. 2. 3. REFERENCE BOOKS: Webster, J.G. 1998. Medical Instrumentation Application and Design. John Wiley and sons, New York. Khandpur R.S. 1997. Handbook of Biomedical Instrumentation. Tata McGraw-Hill, New Delhi, Joseph J. Carr and John M. Brown, 1997. Introduction to Biomedical equipment technology, John Wiley and sons, New York.

MICROBIAL AND FERMENTATION TECHNOLOGY BT-504 Cr L T P 5 3 0 4

Unit I: Introduction to microbial technology: An introduction to microbial world, role of microbial biotechnology at industrial level, fermentation process, the range of fermentation process, microbial biomass, aseptic operation and containment, microbial enzymes, recombinant products, transformation processes, the chronological development of the fermentation industry, The component parts of a fermentation process. Unit II: Microbial metabolism and pathway engineering: Metabolic pathways, glycolysis, pentose phosphate pathway, Entener Doudoroff pathway, TCA cycle, glyoxalate cycle, metabolic Pathway analysis (Typical case study: glutamine biosynthesis), strategies for redirecting branched and linear pathways, alteration of feedback regulation, limiting accumulation of end product, feedback resistant mutants, alteration of permeability, strategies for overproduction of primary metabolites, strategies for overproduction of secondary metabolites, precursor effects, prophophase - idiophase relationship, enzyme induction, feedback regulation. Unit III: Microbial technology: Isolation and improvement of industrially important microorganisms, isolation methods utilizing selection of the desired characteristic, enrichment liquid culture screening methods, improvement of industrial micro-organisms, the isolation of auxotrophic mutants, isolation of resistant mutants, use of recombination systems for the improvement of industrial micro-organisms, application of protoplast fusion and recombinant DNA techniques, the elimination of undesirable products from a production strain. Unit IV: Fermentation technology: Fermentation and types of fermentation, fermenters, microbial cultivation strategies, batch, continuous and fed batch culture system, medium design, media for industrial fermentations; sterilization of air and media, comparison of batch and continuous culture in industrial process, biomass productivity, metabolite productivity, examples of the use of fed-batch culture, scale up and scale down. Unit V: Industrial processes and application: Description of manufacture of enzymes (lipase, xylanase , protease & nucleases), Antibiotics, amino acids, vitamins, ethanol, vaccines (FMD, DTP, New Castle disease), Single cell protein (yeast, spirulina) industrial production of citric acid, lactic acid, modern trends in microbial production of bioplastics (PHB, PHA), microbial fuel cell (MFC), microbial enhanced oil recovery (MEOR).

1. 2. 3. 4. 5.

REFERENCE BOOKS: Stephanopoulos, G., Aristos, A., Aristidou, Nielsen, J.H. and Nielsen, J. 1998. Metabolic Engineering Principles and Methodologies, Academic Press. Bailey J. E. and Ollis D.F. 1986. Fundamentals of Biochemical Engineering (2nd eds.). McGraw Hill, New York. Creuger, W. and Creuger, A. Biotechnology: A textbook of industrial Microbiology. Sinaeur associates. Stanbury, P. F., Whitaker, A. and Hall. S. J. 1995. Principles of Fermentation Technology (2nd eds.). Pergamon Press. Prescott, L. Harley, J. and Klein, D. 2008. Microbiology (7th eds.). McGraw Hill, New York.

BIOCHEMICAL ENGINEERING BT-506 Cr L T P 5 3 0 4

Unit I: Catalyzed enzyme reactions: Reaction mechanisms, comparison with chemical catalysis, Michaelis-Menten and Briggs-Haldane kinetic models and parameter evaluation, enzyme inhibition kinetic models and their implications in elucidating reaction mechanisms and drug design, physico-chemical factors influencing enzyme activity, enzyme deactivation and kinetic models, immobilized enzyme technology, immobilized enzyme kinetics; analysis of external and intra-particle mass transfer; immobilized enzyme reactors various configurations, biosensors, commercial production and applications Unit II: Cell kinetics and bio-reactor design: Kinetics of microbial growth, substrate utilization and product formation Unit III: Heat transfer: Heat transfer in biological systems and sterilization kinetics Unit IV: Scale-up principles: Mass transfer in biological systems scale up principles and control of bioprocess, ideal and non-ideal bioreactors, modes of reactor operations: batch, fedbatch and continuous. biomass growth, substrate uptake and product formation: mass and heat balances, structured and unstructured models, bioreactor design and configurations, mass and heat transfer in gassed reactors, immobilized enzyme and cell reactor systems, animal cell culture strategies. REFERENCE BOOKS: 1. Rajiv Dutta. 2008. Fundamentals of Biochemical Engineering, Springer International (Low Cost Indian Edition through Ane Books, New Delhi). 2. Bailey, J.E. and Ollis, D.F. 1987. Biochemical Engineering Fundamentals, McGraw Hill International Edition. 3. Doran, P.M. Bioprocess Engineering Principles, Academic Press. Lab. based on theory syllabus

PRINCIPLES OF MANAGEMENT AND ENTREPRENEURSHIP DEVELOPMENT BT-508 Cr L T P 5 4 1 0

Unit I: Basic management skills Planning, Organizing, Developing, Controlling Unit II: Performance Appraisal Unit III: Regulatory issues, Environmental issues Unit IV: Interpersonal skills and Wealth Creation Process 1. 2. TEXT AND REFERENCE BOOKS: Koontz, H., Weihrich, H. and ODonnell, C. Essentials of Management McGrawHill/Irwin Drucker, P. The Practice of Management. Harper Business.