General information about the entry View entry in simple text format Entry name Primary accession number

integrated into SWISS-2DPAGE on P53_HUMAN P04637 September 1, 1997 (release 6)

2D Annotations were last modified May 15, 2003 (version 2) on General Annotations were last mod January 31, 2008 (version 13) ified on Name and origin of the protein Description Cellular tumor antigen p53 (Tumor suppressor p53) (Phosphoprotein p53) (Antigen NYCO-13). Name=TP53 Synonyms=P53 Homo sapiens (Human) [TaxID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Gene name Annotated species Taxonomy References [1]

MAPPING ON GEL MEDLINE=97295311; PubMed=9150953; [NCBI, ExPASy, EBI, Israel, Japan] Sanchez J.-C., Wirth P., Jaccoud S., Appel R.D., Sarto C., Wilkins M.R., Hochstrasser D.F. ''Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots'' Electrophoresis 18:638-641(1997) MAPPING ON GEL Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F. Submitted (JAN-1999) to the SWISS-2DPAGE database

[2]

P53_HUMAN (P04637) Number of amino acids: 9 Molecular weight: 1150.2 Theoretical pI: 3.49

CSV format
Amino acid composition: Ala (A) 0 0.0% Arg (R) 0 0.0% Asn (N) 0 0.0% Asp (D) 2 22.2% Cys (C) 0 0.0% Gln (Q) 1 11.1% Glu (E) 1 11.1% Gly (G) 0 0.0% His (H) 0 0.0% Ile (I) 1 11.1% Leu (L) 0 0.0% Lys (K) 0 0.0% Met (M) 0 0.0% Phe (F) 1 11.1% Pro (P) 1 11.1% Ser (S) 0 0.0% Thr (T) 1 11.1% Trp (W) 1 11.1% Tyr (Y) 0 0.0% Val (V) 0 0.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 (Z) 0 (X) 0 0.0% 0.0% 0.0%

Total number of negatively charged residues (Asp + Glu): 3 Total number of positively charged residues (Arg + Lys): 0 Atomic composition: Carbon C Hydrogen H Nitrogen N Oxygen O Sulfur S 53 71 11 18 0

Formula: C53H71N11O18 Total number of atoms: 153 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 5500 Abs 0.1% (=1 g/l) 4.782 Estimated half-life: The N-terminal of the sequence considered is P (Pro). The estimated half-life is: >20 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). ? (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 70.73 This classifies the protein as unstable. Aliphatic index: 43.33 Grand average of hydropathicity (GRAVY): -1.100

FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT

CHAIN DNA_BIND REGION REGION REGION REGION REGION REGION REGION REGION REGION REGION REGION REGION REGION REGION MOTIF MOTIF MOTIF HELIX HELIX HELIX HELIX TURN STRAND STRAND TURN STRAND STRAND STRAND HELIX HELIX STRAND STRAND STRAND TURN STRAND STRAND TURN STRAND STRAND HELIX STRAND HELIX

1-393 102-292 1-83 1-44 66-110 100-370 113-236 116-292 241-248 256-294 273-280 300-393 319-360 325-356 359-363 368-387 305-321 339-350 370-372 19-23 36-38 41-44 47-55 105-108 110-112 123-127 128-131 132-135 141-146 156-165 166-168 177-180 187-189 194-199 204-207 209-211 214-219 228-236 243-248 251-258 264-274 278-287 327-334 335-354

Cellular tumor antigen p53. Interaction with HRMT1L2. Transcription activation (acidic). Interaction with WWOX. Interaction with HIPK1 (By similarity). Required for interaction with FBXO42. Interaction with AXIN1 (By similarity). Interacts with the 53BP2 SH3 domain. Interaction with E4F1. Interaction with DNA. Interaction with CARM1. Interaction with HIPK2. Oligomerization. Interaction with USP7. Basic (repression of DNA-binding). Bipartite nuclear localization signal. Nuclear export signal. [KR]-[STA]-K motif.