Scribd Logo
Carica

Benvenuto in Scribd!

  • Determination of KLa

    Caricato da

    Suraj Lal Gupta
    1K visualizzazioni2 pagine
    The document outlines a procedure to determine the KLa value in a fermenter using the static gas out method. Key steps include adding corn steep liquor to the fermenter, adjusting system variables, sparging nitrogen gas to reduce DO to 0%, then sparging air and taking repeated DO measurements over time up to 80% saturation. KLa is then calculated from the DO readings using a least squares fitting method in Microsoft Excel.

    Descrizione originale:

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Formati disponibili

    DOCX, PDF, TXT o leggi online da Scribd

    Hai trovato utile questo documento?

    Questo contenuto è inappropriato?

    Segnala questo documento
    The document outlines a procedure to determine the KLa value in a fermenter using the static gas out method. Key steps include adding corn steep liquor to the fermenter, adjusting system variables, sparging nitrogen gas to reduce DO to 0%, then sparging air and taking repeated DO measurements over time up to 80% saturation. KLa is then calculated from the DO readings using a least squares fitting method in Microsoft Excel.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Scarica in formato DOCX, PDF, TXT o leggi online su Scribd
    Segnala contenuti inappropriati
    1K visualizzazioni2 pagine

    Determination of KLa

    Caricato da

    Suraj Lal Gupta
    The document outlines a procedure to determine the KLa value in a fermenter using the static gas out method. Key steps include adding corn steep liquor to the fermenter, adjusting system variables, sparging nitrogen gas to reduce DO to 0%, then sparging air and taking repeated DO measurements over time up to 80% saturation. KLa is then calculated from the DO readings using a least squares fitting method in Microsoft Excel.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Scarica in formato DOCX, PDF, TXT o leggi online su Scribd
    Segnala contenuti inappropriati
    di 2

    Determination of KLa

    Objective: To determine KLa value in a fermenter at given impeller rotational speed and gas flow rate using static method of gassing out.

    Apparatus required:
    25 L aerated stirred fermenter DO2 probe and sensor Temperature probe and sensor pH probe and sensor N2 gas cylinder Rotameter Tachometer stop watch Materials required: Corn Steep Liquor 300 ml DM water 14.7 L

    System variables: Temperature 37 oC pH 7.0 Impeller rotational speed 100 rpm Gas flow rate 1 vvm Pressure 1 atm., gauge

    Procedure: 1. 15 L of CSL (5% w/w) is poured into the fermenter. System variables are adjusted as per specified values above. The vessel is aerated at 1 vvm and stirred at 100 rpm for 30 minutes. 2. DO2 probe is calibrated. 3. N2is flushed such that Dissolved oxygen reads 0%. 4. Air is sparged into the vessel at 1 vvm and pressure is adjusted to 1 atm., gauge. 5. As soon as all the specified conditions have been restored, DO2 reading is taken for time t=0. 6. After every 15 seconds readings are taken until the dissolved oxygen reaches 80%. 7. If the reading changes slowly, interval of time can be increased accordingly. 8. Graphical analysis is done using Microsoft excel and KLa value is obtained using least square fitting method.

    Observations: Serial No. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. Time (s) Pressure (kg/cm2, gauge) Rotational speed (LPM) Gas flow rate (vvm) DO (%)

    Potrebbero piacerti anche