Dave Jacobs

110 Lazy Oak Drive * New Market, AL * 35761

(256) 852-4353 * dj1126db8@westpost.net
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Analytical and Biochemical Scientist
Journeyman pharmaceutical and biotechnical scientist with experience in
academic and industrial research, manufacturing, method and process
development and validation. Broad background of analytical chemistry,
biochemistry, molecular biology and genetics. Strong working knowledge of
GMP/GLP. Proficient with advanced instrumentation and in basic bench
chemistry. Routinely multitasks and meets deadlines. Excels at interacting
with coworkers of all levels.
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Core Competencies
HPLC * LC/MS * GC * X-ray diffraction * Differential scanning calorimetry
Dynamic vapor sorption * Moisture analysis * Particle size analysis * Dissolutio
n
PCR * Subcloning * Protein expression and purification * Enzyme assays
Immunoassays
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Professional Experience
Quality Control Chemist, Qualitest, Inc.
Huntsville, Alabama 2009-2010
*HPLC, GC, manual and potentiometric titrations, DSC; various USP monographs
used to measure physical characteristics and verify integrity and identity of
raw pharmaceuticals and excipients.
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Analytical Research Scientist, Westaff, Inc. for KV Pharmaceuticals
St. Louis, Missouri 2008-2009
*Wet bench chemistry and HPLC analysis of in-process materials, stability
formulations and finished pharmaceuticals, including controlled substances
in tablet, liquid and cream formulations.
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Scientist II-IV, Manpower Professional for Pfizer, Inc.
St. Louis, Missouri 2004-2008
*Streamlined the process of characterization, purification and distribution
of synthetic organic compounds by absorbing the partial or complete functions of
five colleagues, freeing others to work on special projects and reducing
departmental outlay on headcount.
*Used robotic liquid handling systems and multiplexed HPLC/MS in high
throughput characterization and purification of newly synthesized candidate APIs
.
*Finished construction, directed programming and initiated operation of a salt
selection/stable form screening system composed of a robotic liquid handler and
PXRD unit.
*Used HPLC, PXRD, DVS, TGA, DSC, cIEF, and dissolution analyzer to evaluate
purity and physical characteristics of candidate APIs; HPLC method development.
*Developed and implemented ELISAs for CTX-1 and osteocalcin to track bone
remodeling in laboratory test animals' sera.
*Established productive relationships with vendors, CROs, shippers and customs
officials to coordinate shipping, receiving, distribution, testing and storage
of biologic APIs.
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Biologist II, Today's Staffing for Monsanto, Inc.
St. Louis, Missouri 2002-2004
*Developed methods to produce and purify recombinant proteins and extract
and purify its analog from plant tissues; AEX and HIC chromatography,
SDS-PAGE,western blots.
*Antisera purification and characterization, ELISA development.
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Senior Research Technician, Washington University School of Medicine
St. Louis, Missouri 1996-2002
*Developed enzyme assay that led to NIH funding, produced and purified
substrates and antibodies, generated transgenic C. elegans strains for in situ
hybridization and fluorescence microscopy. DNA purification, PCR, sequencing,
subcloning and site-directed mutagenesis; SDS-PAGE, western blots.
*Assay/protocol development, data analysis, oral presentations, radiation safety
officer, maintenance of plasmid database and frozen stocks, ordering supplies,
coordination of equipment service.
*Conducted primary research that netted $500,000 in grant money.
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Manufacturing Group Leader/Validation Associate, Alpha Therapeutic Corporation
Los Angeles, CA 1991-1995
*Supervision and training of technicians, method and equipment validation,
ensure daily compliance with cGMP's.
*Protein purification by affinity chromatography, precipitation, and membrane
filtration.
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Publications
Jacobs, et. al., 1998 Gain-of-Function Mutations in the Caenorhabditis elegans
lin-1 ETS Gene Identify a C-Terminal Regulatory Domain Phosphorylated by ERK
MAP Kinase.
Genetics 149: 1809-1822
Jacobs, et. al., 1999 Multiple Docking Sites on Substrate Proteins Form a
Modular System that Mediates Recognition by ERK MAP Kinase.
Genes and Development 13:163-175
Fantz, et. al., 2001 Docking Sites on Substrate Proteins Direct Extracellular
Regulated Kinase to Phosphorylate Specific Residues.
Journal of Biological Chemistry 276: 27256-27265
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Symposia Presentations
"The lin-1 ETS Protein is Phosphorylated by ERK MAP kinase,"
1998 Midwest C. elegans Meeting, University of Chicago, Chicago, Illinois
"A Modular System of Docking Sites Mediates ERK MAP Kinase Recognition of
Substrate Proteins Such as lin-1 and ksr-1"
12th International C. elegans Meeting, University of Wisconsin-Madison
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Education
B. S. Physics, Magna cum Laude, 1989
University of Missouri-St. Louis
Graduate studies in Physics, 1989-1990
University of California, Irvine