Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
Gateway cloning
Microscopy (in vivo imaging using fluorescence and confocal, light microscopy, t
ransmission electron microscopy, bimolecular fluorescence complementation [BiFC]
, slide preparation, sample sectioning)
Bacteria, yeast cultures, and transformation
Yeast one-hybrid (Y1H) and two-hybrid (Y2H) screenings
Flow cytometry
Arabidopsis plant transformation (floral dip), tobacco leaf agroinfiltration, bi
olistics
Arabidopsis mutant analysis, development of silencing lines, genotyping, crosses
Standard molecular techniques (PCR, quantitative PCR (qPCR), RNA extraction, cD
NA synthesis, DNA isolation)
PROFESSIONAL RESEARCH EXPERIENCE
UNIVERSITY OF ARIZONA - Tucson, AZ
Postdoctoral Research Associate, School of Plant Sciences, Schumaker Laboratory
July 2010-Present
- Demonstrating knowledge on plant adaptation to salinity by characterizin
g the evolution and function of the SOS1 gene in Arabidopsis and Thellungiella a
nd by exploiting natural variation in Arabidopsis to identify alleles underlying
salt tolerance
- Preparing and conducting training on several techniques to colleagues, s
uch as yeast work, Gateway cloning technology, and subcellular localization thro
ugh transient expression in tobacco leaves
- Establishing and maintaining collaborative efforts with numerous researc
hers in Belgium, the United States of America, Switzerland, Brazil, and Germany
VIB AND GHENT UNIVERSITY - Ghent, Belgium
Postdoctoral Research Associate, Departments of Plant Systems Biology and Plant
Biotechnology and Genetics April 2008-June 2010
- Conceptualized and developed the characterization of novel plant cell-cy
cle inhibitors induced by stress and upheld the functional study of the vesicula
r trafficking AtPRA1 gene family
- Collaborated in the study evaluating the importance of A-type cyclin-dep
endent kinase CDKA;1 dephosphorylation in plants; conducted a wide range of expe
rimentations, such as Y1H, Y2H, BiFC, overexpression analysis, knockout analysi
s, silencing lines, spatial control of gene expression, plant crosses, and pheno
typic analysis of plants grown in different genotoxic stresses, subcellular loca
lization in stable Arabidopsis plants, and flow cytometry
- Coached and counseled PhD and Master students in performing various labo
ratory experiments; as well as mentored a technician in executing Gateway clonin
g and other laboratory techniques