Curriculum Vitae

Ralf Joseph Carrillo

9200 Jacaranda Way
Gilroy, Ca 95020
rc8b7f6a@westpost.net
Area of Interest:
The Physics and Chemistry of Biological Phenomena
Objective:
My main objective is to obtain a research position in the fields of biophysics a
nd or molecular biology with a focus on the physical principles of the architect
ure of biological macromolecules, proteins, nucleic acids and their complexes, n
amely the energetics of formation of their unique three-dimensional structures.
Education:
Ph.D. Cell Molecular Developmental Biology and Biophysics, CMDB Program, Johns H
opkins University, August 2010.
B.S. Chemistry, University of California at Santa Cruz, June 2005
Publications:
R.J. Carrillo and P.L. Privalov, Unfolding of the bZIP dimers formed by the ATF-
2 and c-Jun transcription factors is not a simple two-state transition, Biophysi
cal Chemistry In Press June 2010
R.J. Carrillo, A.I. Dragan and P.L. Privalov, Stability and DNA-binding ability
of the bZIP dimers formed by the ATF-2 and c-Jun transcription factors. Journal
of Molecular Biology February 2010
A.I. Dragan, R.Carrillo, T.I. Gerasimova and P.L. Privalov, Assembling the Human
Interferon-Beta Enhanceosome in Solution. Journal of Molecular Biology. Septemb
er 2009
Laboratory Skills and Research Experience:
Molecular Biology: DNA cloning, PCR polymerase chain reaction, digestions and l
igations of plasmid DNA and PAGE polyacrylamide gel electrophoresis analysis and
photometric analysis of gel images utilizing ImageQuant software.
Purification: Protein expression and purification, (in eColi), of Activating Tr
anscription Factor2, proto-oncogene c-Jun, histone HMFB variant, and P65 utilizi
ng FPLC and HPLC chromatography with anionic, cationic and hydrophobic exchange
columns. Purification of Interferon 3 utilizing amylose beads and Internalin B a
nd Yeast RNA lariat debranching enzyme (Y-dbr) utilizing nickel-imidazole and si
ze exclusion columns. Purification of small stretches of nucleic acid sequences
and formation of various DNA duplex binding sites. Construction of homo and hete
ro dimer proteins via engineered disulfide bridges.
Biophysical: quantitative characterization of all protein thermodynamic properti
es associated with dimerization, folding and DNA binding, (ie Cp partial specifi
c molar heat capacity, H enthalpy, S entropy and G Gibbs free energies) utilizi
ng: DSC differential scanning calorimetry, ITC isothermal titration microcalorim
etry, CD circular dichroism and Fluorescence Anisotropy.
Computational: Development and implementation of mathematical expressions to gr
aphically simulate or fit experimental data and explore the theoretical origin o
f the experimental observation. These algorithms were developed for the Origin
platform, but they are general expressions which can be adapted to any other sof
tware platform that both calculates and renders a graphical output. Experience w
ith Origin 8.0, Bindworks, Cp Calc, ImageQuant and MS Office (Word Excel and Pow
erPoint).
Instrument Experience:
ITC Isothermal titration microcalorimetry
DSC Differential scanning microcalorimetry
CD Circular Dichroism
Fluorescence Anisotropy
Ultraviolet-Visible Spectrophotometry
HPLC and FPLC chromatography
Additional Information:
Membership:
SACNAS, Society for the Advancement of Chicanos and Native Americans In Science
IA Phi Iota Alpha The oldest Latino fraternity in the United States
Honors and Awards:
MARC, Minority Access to Research Careers fellowship 2004/2005 UCSC
CAMP California Alliance for Minority Participation scholarship 2003/2004 UCSC
NSF, National Science Foundation, special merit award for poster presentation o
f Architecture at the Core of the Spliceosome in the physical sciences and engi
neering division at the Beckman Institute 2004.
Presentations:
Johns Hopkins CMDB Graduate Retreat, Maryland 2007
Institute for Biophysical Research, Baltimore Maryland 2006
SACNAS National Conference, Santa Fe New Mexico 2005
CAMP California Alliance for Minority Participation conference, Irvine Californi
a 2004
References:
Dr. Evangelos Moudrianakis vanm@jhu.edu
Dr. Anatoliy Dragan dragan@umbi.umd.edu
Dr. Hem Shukla hshukla2@jhu.edu