vs575c12@westpost.net 617-331-5079(C) xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Executive Summary ----------------- 5yr of Molecular, in vitro and cell- based assays developmentexperience in cancer/ virology. More than 2 years of industry experiencein ISO 9001:2008 certified company. Proficient invarious molecular biology, virology, immunology and bioinformatic tools. xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx WORK EXPERIENCE --------------- -Beckman Coulter Genomics, Danvers, MA Jan 2010- present -Assay Development Lead Involved in optimization of HIV detection from clinical samples using bead based technology and quantitative real time RT-PCR. -Beckman Coulter Genomics, Danvers, MA. Apr, 2008- 2009 -Senior Development Scientist Invented transition metal based RNA extraction process from clinical samples and optimized RNA extraction for an easy automation. Developed molecular diagnostic assay and process controls for detection of Epstein Barr virus infection. Optimized DNA, RNA amplification chemistry using proprietary DNA polymerases. Made the company save $10,000/mo by optimizing Realtime PCR assays with heat stable Uracil DNA Glycosylase. -Eutrophics Pharmaceutucals, MA. Jul, 2006-2007 -Assay Development Consultant Developed cell based in vivo assays to screen for apoptotic inducing small molecules. Worked on Fluorescence polarizationbased assay for small molecule screen against antiapoptotic proteins. -Beth Israel Deaconess Medical Center, MA Feb-Apr,2008 -Postdoctoral Fellow As part of evaluating HIV vaccine clinical trials in Monkeys,mice and human beings, worked on identifying specific cell surface biomarkers involved in mucosal immunity using FACS multiplex assay. -Brigham and Women's Hospital, Boston, MA Dec, 2002-2008 -Postdoctoral Fellow Developed luminescence based HTS cell based assay to screen for small molecules against poxvirus replication. Also, developed luminescence based reporter assays involved in transcriptional regulation of host genes. Worked on elucidating the role of Kaposi's Sarcoma associated herpes virus proteins in tumorigenesis. Developed radioactive based medium throughput assay to identify amino acid residues which would disrupt viral protein-DNA interaction involved in latency. xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx RESEARCH Technical Skills ------------------------- Standard molecular biology techniques such as DNA/RNA purification,PCR, RT-PCR, Realtime PCR, cloning, DNA/RNA hybridization, basic bioinformatics. Screening of small molecules and peptides in vitroand in cell based assays. Recombinant protein expression and affinity purification of proteins in bacteria, vaccinia (pox), insect cells. Basic cell culture of primary and secondary cell lines,infection, propagation of all animal viruses. Immunoassay such as ELISA, neutralization tests, immunofluorescence, proliferationassays,immunoprecipitation, cytotoxicity assays,tetramer staining, ICS, multi fluorimetric staining FACS analysis. DOE and various statistical analysis. Conductinganimal model studies in Monkeys, Mice, Chickens and Guinea pigs. xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Managerial Experience --------------------- Managed and worked as team to develop molecular diagnostic tools, Radiation and Bio-safety Officer. General laboratory administration.Assisted and managed technicians, graduate students, postdoctoral fellows, and summer interns in research towards completion of research projects. xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Professional Board Certifications --------------------------------- -American College of Veterinary Microbiologists. USA, Diplomate, Virology 2007 -American Society for Virology, USA, Member 2008 Education --------- -PhD, University of Illinois, Pathobiology (Micro & Immuno) GPA.3.94 Dec, 2002 -M.V.Sc (M.S),Biotechnology, Indian Veterinary Research Institute GPA.8.4 Jul,1998 -B.V.Sc,(B.S) Veterinary Science,Madras Veterinary College GPA. 3.54 May, 1994 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx SELECTED Research Publications * Authors contributed equally to the work 1. Srinivasan V., Newcomb K., Barbera AJ., Ballestas M.E., Kaye K. (2010). Episome maintenance function of Latency-Associated Nuclear Antigen of Kaposi's sarcoma- associated herpes virus is mediated by evolutionarily conserved mechanisms. Manuscript in preparation for J Biochem. 2. Kelly-Clarke B., Ballestas ME, Srinivasan V, Barbera AJ, Komatsu T, Harris T, Kazanjian M, Kaye KM (2007). Separationof the essential KSHV C-terminal LANA domain into chromosomebinding and DNA recognition subdomains. J Virol 81; 4348-4356. 3.You J*., Srinivasan V*., Denis G., Harrington Jr W., Kaye K., and Howley P. (2006) The Kaposi's Sarcoma- AssociatedHerpesvirus Latency-Associated Nuclear Antigen interacts with bromodomain protein Brd4 on host mitotic chromosomes. J Virol 80; 8909-8919. 4. Srinivasan V., Schnitzlein W.M., Tripathy D. (2006). Genetic manipulation of two fowlpox virus late transcriptionalregulatory elements influences their ability to direct expression of foreign genes. Virus Res 116; 85-90. 5. Srinivasan V., Tripathy D. N. (2005). The DNA repair enzyme, CPD-photolyase restores the infectivity of UV-damaged fowlpox virus isolated from infected scabs of chickens. Vet Micro 108; 215-223. 6. Srinivasan V*., Komatsu T*., Ballestas M.E., Kaye K. (2004). Definition of non-palindromic sequence requirements for LANA1 binding to KSHV DNA. J Virol 78; 14033-38. 7. Srinivasan V., Schnitzlein W. M., Tripathy D. N. (2003). A consideration of previously uncharacterized fowlpox virus unidirectional and bi-directional late promoters for inclusion in homologous recombinant vaccine. Avian Diseases 47; 286-295. 8. Srinivasan V., Schnitzlein, W. M., Tripathy D. N. (2001) Fowlpox virus encodes for a novel DNA repair enzyme that restores infectivity of UV-light damaged virus. J Virol 75; 1681-88. 9. Suryanarayana V. V. S. , Srinivasan V., Ratish G. (1999) E-coli expressed proteins as diagnostic reagents for typing of foot and mouth disease virus. Arch Virol 144; 1701-1712. xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
The International Journal of Biochemistry & Cell Biology Volume 76 Issue 2016 (Doi 10.1016/j.biocel.2016.04.014) Lofrumento, Dario Domenico La Piana, Gianluigi Palmitessa, Val - Stimulation by PR