RESEARCH ARTICLE

Antimicrobial Activities of the Petroleum Ether, 0.1ml) for fungi were used as positive control
for comparison of the activities.
Chloroform, Methanol and Water Extracts of Eclipta
RESULTS AND DISCUSSION
alba L. Plant All the extracts were exhibited good
Mithun N M 1*, Shashidhara S1, Deepak M 2 antibacterial and antifungal activity.
Petroleum ether extract and chloroform
Abstracts: Petroleum ether, chloroform, methanol and aqueous extracts of Eclipta alba L. extracts were shown significant activity
whole plant were evaluated for antibacterial activity against E.coli, S.typhi, S.aureus, B.subtilis against E. coli ad S. typhi compared to other
and antifungal activities against Candida albicans and Aspergillus niger with the comparison extracts. Methanolic extracts was shown
with positive control (Ciprofloxacin and Amoxicillin) for bacteria and (Ketoconazole and highest activity against B. subtilis and S.
Fluconazole) for fungi. Petroleum ether extract and methanolic extracts were found to be more aureus as compared to other extracts.
significant active compared to other extracts for bacteria and methanolic extract shown Chloroform and aqueous extracts were found
significant inhibition for fungi. to shown significant inhibition in Candida
Key Words: Antimicrobial, Antibiotic, Eclipta alba, Antifungal. albicans compared to Aspergillus niger. The
zone of inhibition is given in Table No.1.
INTRODUCTION water for 24 hours and filtered. The extracts
Eclipta alba L. belonging to family Astraceae, were air dried after filtration to concentrate. From the comparison of the plant
it is commonly called as Bhringaraj. The The extracts were dissolved in Dimethyl extracts activity, positive control
plant is distributed throughout India in wet formamide (DMF) for antibacterial activity (ciprofloxacin and amoxicillin for bacteria)
or moist wasteland ascending up to 2000M and dimethyl sulfoxide (DMSO) for antifungal and (Ketoconazole and Fluconazole for fungi)
on the hills4. Its major chemical constituents activity in different test tubes, the final were used and negative control (only solvent,
are Coumestan derivatives, Wedelolactone concentration was 1000mcg/0.1ml. Dimethyl formamide for bacteria and
0.5-0.55%, Demethyl wedelolactone and Dimethyl sulfoxide for fungi) was used. The
Demethyl-wedelolactone-7-glucoside and Antibacterial and Antifungal Screening positive control showed significant
sulphur containing peptides1. Alkaloids like Four bacteria, (Two gram +ve i.e. B.subtilis, S. antibacterial activity against the all bacteria.
ecliptine and nicotine5, Saponins like aureus and two gram-ve i.e. E. coli, S. typhi)
eclalbosaponins6. And other and two fungi (Candida albicans and CONCLUSION
phytoconstituents like alpha- Aspergillus niger) were used in the present As petroleum ether, chloroform, methanol
terthienymethanol, Tannins, vitamin-A, study to determine the antibacterial and and aqueous extracts exhibited an antibiotic
Eclalbatin2, ursolic acid and oleanolic acid, antifungal activity of the crude extracts by potential against all the organisms, it proved
daucosterol3. It is one such plant whose agar diffusion method (cup plate method). In that traditional use of Eclipta alba L. has
whole plant and leaves are used not only as the agar diffusion method, nutrient agar for scientific basis. Further investigation is
food but also as an ingredient in traditional bacteria and Sabouraud dextrose agar for necessary to find out the active compounds
medicine7. It is reported to possess fungi were used as culture media and cavity and to confirm their bioactive principles.
Hypoglycemic8, Antiproliferative9, were aseptically made over the culture plates
Hepatoprotective11, Antiradical and using borer (9mm internal diameter). The REFERENCES AND NOTES
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whole plant.
Table 1: Record of zone of Inhibition in mm of petroleum ether, chloroform, methanol
Materials and methods and aqueous extracts of whole plant of Eclipta alba L. against various organisms.
Plant Material Asper-
The dried whole plant of Eclipta alba L. were Concen S. B. S. Candida
Sl. Name of E.coli gillus
obtained from Natural Remedies PVT LTD, tration typhi subtilis aureus albicans
No. the Drug mm niger
Bangalore and authenticated by National (µg/disc) mm mm mm mm
mm
Institute of Science Communication and
1 Ciprofloxacin 100 41 41 40 32 - -
Information Resources (NISCAIR), New
2 Amoxicillin 100 21 26 27 20 - -
Delhi. The authenticated plant was used for
3 Ketoconazole 100 - - - - 18 18
the successive solvent extraction with
various solvent. 4 Fluconazole 100 - - - - 18 31
Petroleum
5 1000 25 21 16 17 15 17
Preparation of Various Extracts ether extract
The whole plant were cleaned, dried and Chloroform
6 1000 23 18 14 18 16 15
were ground into a coarse powder. 50 gram extract
of the powdered plant was successively Methanolic
7 1000 21 18 17 15 15 17
extracted with petroleum ether, chloroform, Extract
methanol by hot soxhlet method. The Aqueous
8 1000 19 15 15 15 16 13
extraction was carried out for 4 hours for extract
each solvent. The marc left after extraction
with methanol was macerated with distilled The activities were determined by 2. Upadhyay RK, Pandey MB, Jha RN, PandeyVB.
measuring the diameter of the zone in mm. Eclalbatin a triterpine saponins from Eclipta
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1Dept. of Pharmacognosy, Government college 3. Zhang M, Chen Y. Chemical constituents of
of pharmacy, Bangalore, India.
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E-mail: used as negative control in each time. Za Zhi.1996; 21: 480-1.
*Corresponding author Standard Ciprofloxacin (100mcg/0.1ml), 4. Kirtikar KR, Basu BD. Indian medicinal plant,
Amoxicillin (100mcg/0.1ml) for bacteria and 2nd ed, Dehradun: International Book
2 Natural Remedies Pvt. Ltd. Bangalore, India. Ketoconazole and Fluconazole (100mcg/ Distributors; 1998.

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 RESEARCH ARTICLE
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