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ISSN 1992-0067
© IDOSI Publications, 2010
1
Indian Institute of Crop Processing Technology, Thanjavur, India
2
Department of Biotechnology, K.S.R. College of Arts and Science, Tiruchengode, India
Abstract: The medicinal plants are used in traditional treatments to cure variety of diseases for thousands of
year. The aim of this study was to identify such plants with antimicrobial and antioxidant efficacy for controlling
some food borne pathogens. The locally available plants viz., Ficus religiosa, Leucas aspera, Holarrhena
antidysenterica and Psidium guajavai were selected for the study. Various methods were tried to standardize
the extraction of antimicrobial agents. Methanol extraction of P. guajava showed high antimicrobial activity
against the food borne pathogens like S. typi, Pseudomonas spp and B. Subtilis. Whereas in case of antifungal
H. antidysenterica showed more activity with maximum of 23mm inhibition zone against Aspergillus spp. The
high antioxidant activity of 36ìg/100mg was observed in Psidium guajava. The GC-MS study also revealed the
various phytic components for H. antidysenterica, which assured that it has high antimicrobial, anticancer
activity against the food borne pathogens. It was concluded that plant extract can be used as a preservatives
against the food borne pathogens.
Corresponding Author: M. Loganathan, Senior Scientist, Indian Institute of Crop Processing Technology,
Thanjavur -613 005, India
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Advan. Biol. Res., 4 (2): 122-125, 2010
obtain a variety of newer herbal drugs. About 80% of microorganisms. The plates were incubated for 24 to
individuals from developed countries use traditional 48 hours. Similar procedure was carried out for the rest of
medicine, which has compounds derived from medicinal the isolates. The zone of inhibition was calculated by
plants [5]. The present research has been conducted to measuring the diameter of the inhibition zone around the
study the medicinal properties like antimicrobial and well (mm) including the well diameter. The readings were
antioxidant properties of some locally available medicinal taken in three different fixed directions in all 3 replicates
plants against food borne pathogens. and the average value was tabulated.
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Advan. Biol. Res., 4 (2): 122-125, 2010
Fig. 1: Antimicrobial activity of Ficus religiosa Fig. 4: Antimicrobial activity of Psidium guajava
Fig. 2: Antimicrobial activity of Leucas aspera Fig. 5: Antimicrobial activity of all four selected plants
against fungi
Fig. 3: Antimicrobial activity of Holarrhena Fig. 6: Estimation of antioxidant activity for four plant
antidysenterica extract
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Advan. Biol. Res., 4 (2): 122-125, 2010
Methanol extraction of L. aspera showed activity in To avoid these problems, medicinal plants can be used to
Pseudomonas spp and B. Subtilis with 22-23mm, that the prevent the food borne microorganisms as a tool of
aqueous and ethanol extraction showed low activities preservatives during food processing and to design a
(Fig. 2). The methanol extraction of H. antidysenterica drug.
showed high activity on the pathogens above 16mm
inhibition zone. S. typi showed more sustability to the REFERENCES
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