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Martina Lepiešová
All health care professionals’ efforts to minimize the onset and spread of
infection are based upon asepsis and the principles of aseptic technique. An
aseptic environment excludes the presence of any pathogenic (disease-
producing) microorganisms and acknowledges only the minimum amount of
non-pathogenic microorganisms in the air. The two types of aseptic technique
are medical asepsis (clean technique) and surgical asepsis (sterile
technique). The techniques used in maintaining surgical asepsis are more
rigid than those performed under medical asepsis. Surgical asepsis has to be
practiced and maintained not only in operating rooms, delivery areas, burns
units or major diagnostic or special procedure areas but fundamentally also
during the performance of many procedures in general care areas at the
patient’s bedside (e.g. procedures requiring intentional perforation of a
patient’s skin such as insertion of i.v. catheter; procedures involving insertion
of devices or surgical instruments into normally sterile body cavities such as
performing urinary catheterization or when skin integrity is broken due to a
surgical incision or burns). In such situations not all the sterile techniques are
required as in operation rooms, however the medical aseptic practices have to
be applied and the principles of surgical asepsis have to be followed.
Examples of medical aseptic practices to break the chain of infection are
listed in table 2.1 and the basic principles of surgical asepsis and practices
related to each principle are introduced in table 2.2.
Purpose
- to minimize the number of risk factors (particularly external factors)
predisposing a patient to an infectious process by following the principles
of medical and surgical asepsis,
- to remove transient and potentially reduce resident flora of the hands by
performing the proper hand washing technique in relation to the
procedures being undertaken,
- to conduct all procedures appropriately from the perspective of infection
prevention,
- to establish a sterile field prior to all invasive surgical procedures.
Collaborative level
- interdependent
All health care professionals, even students, are responsible for maintaining
medical and surgical asepsis and following their principles in direct or
indirect contact with patients with the aim of not contributing to the onset and
spread of infections (hospital acquired infections / cross infections /
nosocomial infections).
Related procedures
- hand washing procedure,
- establishing and maintaining a sterile field,
- donning and removing sterile gloves.
Note: In all the situations mentioned if hands are not visibly soiled, use only
an alcohol based rub. If hands are visibly dirty or contaminated, they should
be washed first with soap and water [alcohol-based products have been
found to be more effective than plain soaps or antiseptic soaps; moreover
those containing emollients have caused substantially less skin irritation and
dryness than plain soaps or antimicrobial soaps tested].
Proper hand hygiene (hand washing and hand rub procedures) together with
the basic principles of hand care are introduced in table 2.3 and in figure
2.1.
Table 2.3 Hand hygiene – hand care and hand washing procedure
Hand care – checklist Sequence of hand washing procedure (hand
washing / hand rub
- keep the nails short, don’t wear - wet the hands and wrists under running
nail polish (even a transparent warm water
one) or artificial nails - take a dose of soap / hand wash into a
- don’t wear ridged / stoned rings cupped hand – always operate the
(wedding ring is questionable – dispenser’s application with the elbow to
refer to the institutional policy) push the detergent
- remove wrist watches and all - wash hands for 40 – 60 seconds thoroughly
jewellery on all the surfaces of hands by six basic
- roll up / remove long sleeved movements (Figure 2.1) without adding
clothes more water
- keep hands and forearms lower than elbows
- maintain skin intact as far as during washing to facilitate removal of
possible microorganisms
- cover cuts and abrasions with an - rinse hands and wrists thoroughly under
impermeable waterproof plaster running water, keeping hands down and
- don’t use communal pots of hand elbow up
cream (better individual-use hand - don’t turn the taps off with clean hands – if
creams, creams produced elbow or foot control isn’t available, use a
specifically for use after a paper towel to touch and operate the taps
concrete antiseptic alcohol agent) - dry the hands properly with a disposable
- always wear disposable gloves paper towel or a fresh portion of a roller
when handling blood, body fluids towel (no need to use several sheets of
or substances (but never regard disposable paper)
gloves to be a substitute for hand - rub the dose of liquid alcohol agent /
washing!) antiseptic hand rub agent (3 ml by one
push) into the skin of dry hands following
all six basic movements for at least 30
seconds
Note: An alternative method of hand disinfection as already described is the use of
alcohol gels. They are formulated for use without water and are particularly useful in
areas where a hand-washing basin is not readily available, or when return to a hand-
washing basin is impractical e.g. during a ward round, in between bed making, during
a dressing procedure, etc.
Figure 2.1 Six basic movements of hand washing technique to be applied to all
hand surfaces
Equipment
- waist-high table,
- package containing a sterile drape (or sterile kit to be used as a sterile
field),
- sterile equipment and supplies as needed specifically for the procedure
(e.g. sterile surgical instruments – plain dissecting forceps / plain thumb
forceps / tissue forceps / pincette, surgical scissors, surgical spoons,
haemostat / artery forceps / forceps / pean, suture forceps, scalpels, suture
material, sterile dressing material, antiseptic solutions, etc.),
- disposable cap and mask (and/or protective eyewear), sterile gloves,
sterile gown.
Assessment
1. Verify that the procedure requires a surgical aseptic technique.
2. Anticipate the number and variety of supplies needed for the procedure
[failure to have necessary supplies causes you to leave a sterile field,
increasing the risk of contamination].
3. Assess patient’s comfort, oxygen requirements and elimination needs
before preparing the procedure [certain procedures may last a long time].
4. Confirm the sterility of packages
- ensure that package is clean, dry, intact (no punctures, tears or
discolouration), check for sterilization expiration dates, sterilization
indicator [if moist, or there are any indications it has been previously
opened, consider it contaminated and discard it].
Implementation
5. Complete all other priority tasks before beginning the procedure [sterile
field should be prepared as close as possible to the time of its use].
6. Position patient comfortably for a specific procedure with the help of
assistive personnel.
7. Explain the procedure to the patient (purpose, process, importance of
sterile technique) [enables patient to cooperate and eliminates need to
talk during procedure, increasing risk of contamination].
8. Apply cap, mask, and/or protective eyewear and/or gown as needed due to
institutional policy.
9. Perform hand hygiene thoroughly (hand washing and alcohol hand rub).
10. Open the package.
10a) To open a wrapped package:
- place the package in the centre of work area (clean, dry, flat work surface
above waist level) so that the top flap of the wrapper opens away from
you [position like this prevents reaching over the exposed sterile content],
- by reaching around the package (not over!), pinch the first flap on the
outside of the wrapper between the thumb and index finger and open the
flap [touching the outside of the wrapper maintains the sterility of the
inside],
- do the same with side flaps, using the right hand for right flap and vice
versa [using both hands avoids reaching over the sterile contents],
- pull the fourth flap toward you, make sure not to touch the uniform by the
flap.
Note: To open a wrapped package while holding it, hold the package in one
hand (non-dominant) with the top flap of the wrapper opening away from you
and by using the other, dominant hand, open the package pulling the corners
of the flaps.
10b) To open commercially prepared packages:
- if the package has an unsealed corner, hold the package in one hand and
open it by pulling the flap back with the other hand,
- if the package has a partially sealed edge, grasp both sides of the edge,
one with each hand, and gently open by pulling apart.
11. Establish a sterile field by using a drape
- open the package containing the drape, apply sterile gloves (this is
optional according to institutional policy), with dominant hand pluck the
corner of the drape that is folded back on the top and lift the drape out
from the cover allowing it to open freely without touching any objects,
- with the other hand, pick up another corner of the drape, holding it well
away from yourself,
- lay the drape on a clean and dry surface, placing the freely hanging
bottom half farthest from you and then place top half of drape on work
surface [to prevent leaning over the sterile field and contaminating it].
12. Add necessary sterile supplies
- open the package of the supply while holding outside wrapper in non-
dominant hand,
- secure wrapper edges by peeling the wrapper over non-dominant hand,
- hold the package approx. 15 cm above the field and allow the contents to
drop onto the field (in bigger packages with bigger supplies, gently place
the sterile supply from opened package on the sterile field by approaching
from an angle – never reach arms over sterile field).
13. Pour sterile solutions
- remove seal and cup from bottle in upward motion, gently pour solution to
the sterile container (metal / plastic / porcelain bowl or cup) with the
solution bottle held away from sterile field and the bottle lip approx. 10 –
15 cm above the container.
14. Use sterile forceps (handling forceps / transfer forceps) to handle certain
sterile supplies, e.g. to move sterile article from one place to another
- keep the tips of wet forceps (if stored in wet method or made wet during
its use) lower than the handles at all times unless sterile gloved hands
[prevention of flowing the liquids from the tips to the handles and later
back to tips – handles are unsterile if held by the hands],
- hold sterile forceps always above waist level, within sight,
- when using forceps to lift sterile supplies out of the package, be sure they
don’t touch the edges or outside of the wrapper,
- don’t permit moist forceps to touch the sterile field if the surface under
sterile field is unsterile and moisture-proof drape is not used as a barrier.
Complications
- any break in sterile technique (e.g. sterile field has come into contact with
contaminated object, liquids splash onto drape) may lead to contamination
of sterile field thus requiring setting up a new sterile field.
Documentation
- no recording or reporting is required for this set of skills,
- record sterile procedure performed including assessment of patient’s
status prior to and its evaluation during and after the procedure.
Equipment
- package of proper-size sterile gloves, latex or synthetic latex-free [gloves
shouldn’t stretch so tightly over the fingers that they can easily tear, yet
they should be tight enough to handle objects; in relation to allergy to
latex in many health care professionals as well as many patients (with
mild to severe reactions to latex) there should be the possibility to choose
synthetic non-latex gloves note: hypoallergenic, low-powder or low
protein latex gloves still contain latex protein].
Assessment
1. Verify that the procedure requires the use of sterile gloves – consult
institutional policy, consider patient’s risk of infection.
2. Assess patient for the risk factors of he or she being predisposed to latex
allergy reaction (history of asthma, contact dermatitis, rhinitis, food
allergies, previous adverse reactions during surgery or dental procedure,
previous reaction to latex products – adhesive tape, face mask, bandage,
elastic underwear, i.v. tubing, condom, rubber gloves, ostomy bag, etc.) –
choose the correct material.
3. Confirm the sterility of the package.
Implementation
4. Perform hand hygiene thoroughly (hand washing and alcohol hand rub).
5. Donning sterile gloves (Figure 2.2)
- remove the inner glove package from the outer package, place the inner
package of gloves on a clean, dry, flat surface at waist level,
- open inner package by plucking the flaps and folded tabs so that the
fingers do not touch the inner surfaces,
- identify right and left glove (each having a cuff approx. 5 cm wide),
- non dominant hand first: with thumb and first two fingers of your non-
dominant hand, grasp the glove for the dominant hand by the edge of
glove cuff touching only the inside of cuff [inner surface of cuff will lie
against skin and thus is not sterile], insert the dominant hand into the
glove and carefully pull the glove on while keeping the thumb of inserted
hand against the palm [thus the thumb is less likely to contaminate the
outside of the glove] leaving cuff and being sure cuff doesn’t roll up wrist,
- with gloved dominant hand pick up the other glove, inserting the gloved
fingers under the cuff and holding the gloved thumb close to the gloved
palm [prevention of accidental contamination of the glove by the bare
hand] and pull on the second glove carefully keeping the thumb of
dominant hand as far as possible from the palm (abducted back),
- after second glove on, adjust each glove so that it fits smoothly e.g. by
interlocking the hands together above the waist level (the cuffs usually fall
down – if not, carefully pull cuffs up by sliding the fingers under the cuffs
touching only sterile sides).
Complications
- any break in sterile technique (e.g. contamination of glove during its
donning by the bare hand, contamination of sterile gloved hands by
touching contaminated or even clean objects, by incorrect position of
sterile gloved hands – e.g. below waist level, by developing a tear in a
sterile glove) requires application of new sterile gloves immediately.
Documentation
- no recording or reporting is required for application of gloves; record and
report possible latex allergy reaction – patient’s response, vital signs,
treatment applied and reaction to it,
- record sterile procedure performed including assessment of patient’s
status prior to and its evaluation during and after the procedure.
Test your knowledge
1. What is the difference between medical and surgical asepsis?
2. Summarize the principles and rule of hand care except hand washing.
3. When to use different types of hand washing?
4. What is the time exposure to antiseptic hand rub agent in hygienic hand
disinfection and in surgical hand disinfection?
5. Name 3 examples of principles of surgical asepsis and practices for each
principle.
6. Describe and explain how to hold wet sterile handling forceps during
establishing a sterile field while adding necessary sterile supplies.
7. Name risk factors predisposing the patient to latex allergy reaction.
Purpose
- to identify types of wounds and appropriate treatment options for certain
types,
- to assess and report all the characteristics of a wound properly including
wound healing process,
- to promote wound healing and minimize the risk of complications,
- to perform specific procedures of wound care correctly (e.g. removing
soiled dressing, wound cleansing (cleaning, irrigation), applying a sterile
dressing, suture removal, care of a wound-drainage system),
- to prevent infection.
Collaborative level
- interdependent
Nurses usually assist the physicians in wound management (wound
scrubbing, suture removal and removal of a drain should be performed only
by the surgeons). An initial surgical wound dressing is usually not removed
until a physician decides to remove it and inspects the wound. In the case of a
regular need to cleanse the wound or change its dressing (e.g. if excessive
amount of drainage occurs), nurses perform these procedures themselves. The
nurse’s responsibility is to assess and document wound characteristics.
Moreover, nurses are responsible for patient education particularly
concerning chronic wound management.
Related procedures
- cleaning a wound,
- applying a sterile dressing.
clean it from top to bottom start at the centre moving start in the area
toward one end, then start at immediately next to the
the centre again and work drain (with each new
toward the other end, swab attempt to clean a
continue with other little further out from
movements in straight lines the drain)
moving away from the
suture line
Wound dressings are applied for the following purposes – to protect the
wound from mechanical injury and microbial contamination, to maintain
humidity, to absorb drainage and/or debride a wound, to provide thermal
isolation, to prevent haemorrhage (if pressure dressing is applied), to
immobilize the wound (thus facilitate healing and prevent injury) and to
provide psychological / aesthetic comfort.
There are different types of dressings – traditional dressing is gauze (the
modes of applying gauze dressings are: dry-to-dry, wet-to-dry, wet-to damp
and wet-to-wet dressings) and there are new synthetic modern dressings to
provide moist wound healing (occlusive / semiocclusive, transparent adhesive
films, impregnated nonadherent dressings, hydrocolloids, hydrogels,
polyurethane foams, alginates, exudate absorbers, etc.).
The layers of dressing are primary layer (contact, under) represented usually
by a wet sterile gauze square, secondary layer (absorbent, outer, cover)
composed of dry gauze squares, surgipads or cotton wool layer if wound
drainage is excessive and fixation layer, performed by e.g. tapes (elastic
adhesive tape, nonallergenic tape) or bandages / binders, securing the
dressing not to become dislodged. The principles of securing dressings are
listed in table 2.8.
Not all surgical dressings require changing – sometimes the dressing remains
in place until the sutures are removed; however, most surgical dressings are
changed regularly to prevent the growth of microorganisms.
Figure 2.4 Securing the wound dressing by a tape (correct and incorrect way)
Equipment
- disposable / sterile gloves [disposable to protect yourself during removal
of soiled dressing; sterile to examine the depth of a wound / to hold sterile
dressing supplies], other PPE – mask, protective eyewear, gown
[optional],
- sterile handling forceps in a sterile cylinder / jar [to handle sterile
instruments or sterile dressing supplies],
- sterile cassette with sterile surgical instruments (e.g. forceps to hold
cleansing swab; probe to assess the wound depth; surgical spoon / scalpel
for mechanical debridement / surgical necrectomy; suture forceps to make
sutures; surgical scissors to cut the sutures / sterile dressings / to shorten
the drains, etc.),
- sterile cleansing solutions and irrigation delivery system if irrigation is to
be performed (sterile pink needle, sterile syringe, sterile catheter) [to
withdraw solution from a sterile bottle and apply it to/on the wound],
- dressing supplies – sterile gauze swabs, sterile gauze squares in sterile
packages (paper wrapper – Lucasteric sac / paper-folic wrapper / bowls /
cassettes) or commercially prepared sterile packages of synthetic modern
dressings,
- clean bandage scissors [to cut the packages, to cut the bandages],
- adhesive tapes / bandages of proper width / binders [to secure the new
dressing],
- clean kidney dish / collection basin and disposable waterproof biohazard
bag,
- folded paper towels or extra moisture-proof underpad [to protect bed /
patient’s personal clothes / pyjama],
- extra bath blanket [to cover patient’s intimate parts when needed],
- additional supplies required (e.g. extra absorbent dressings – surgipads,
folded cotton wool, ordered ointments, powders, etc.).
Assessment
1. Review physician’s orders concerning wound cleansing / any other wound
care procedure (increased frequency of cleansing, specific supplies
required, what to report, etc.).
2. Assess recent recording of any characteristics related to patient’s wound
to get prepared for the procedure and to identify the changes in wound
condition after removal of soiled dressing (extent of integrity of skin
impairment; wound location; wound size in length, width, depth; signs of
infection – e.g. elevation of body temperature, wound odour, wound
colour; wound drainage as for amount, colour, consistency, type – serous,
sanguineous, serosanguineous, purulent; stage of wound healing; wound
dressing – if clean, dry, evidence of bleeding, profuse drainage; identify if
patient is at risk of wound healing problems).
3. Assess patient’s history of allergies to antiseptic solutions, tapes or
dressing materials, assess the risk factors predisposing for latex allergy
reaction – modify the selection of supplies to use the correct material.
4. Assess patient’s comfort level or pain (on numeric scale of 0 to 10),
symptoms of anxiety, elimination needs before preparing the procedure
[discomfort may be related directly to wound, anxiety to anticipation of
unknown procedure; certain procedures may last a long time].
5. Confirm the sterility of all packages and sterile supplies.
Implementation
6. Explain the procedure to the patient (purpose, process, importance of
sterile/clean technique) [to reduce anxiety, to enable patient to
cooperate].
7. If necessary, apply premedication (prescribed analgesic) 30 to 45 minutes
prior to the procedure [to ensure patient’s comfort positioning, to enable
patient to cooperate].
8. Position patient comfortably for specific wound care procedure, expose
the wound and place extra towels and kidney dish close to the wound area
to protect bedding and collect any possible exiting fluids [to permit
gravitational flow of possible excessive drainage or irrigating solution
into this collection basin].
9. Close the room door / bed curtains, use extra bath blanket to cover the
patient if needed.
10. Prepare a waterproof bag for disposal of soiled dressings within reach,
make cuff on it.
11. Perform hand hygiene thoroughly (hand washing and alcohol hand rub).
12. Apply disposable gloves and/or other PPE as needed due to institutional
policy.
13. Remove soiled dressings appropriately
- untie binders or bandages / cut them with bandage scissors (but never
directly above the wound) / remove adhesive tapes; remove all the
dressing layers always with underside away from patient’s face [not to
make him/her upset], taking care not to dislodge any drains while
removing the under layer [if gauze sticks to drain, support the drain with
one hand while removing the dressing; if dressing material adheres to any
tissue during removal, soak it with normal saline],
- assess amount, type and odour of wound drainage based on the soiled
dressings,
- discard the soiled dressings including gloves used in waterproof bag.
14. Wash the hands.
15. Assess the characteristics of wound / wound healing [if assessing includes
touching the wound, e.g. examining the depth of wound, put on sterile
gloves].
16. Describe the appearance of the wound and indicators of wound healing to
the patient.
17. Set up sterile supplies (using surgical aseptic technique)
- by using handling forceps draw up the forceps / any other instrument from
the cassette always catching the middle part of an instrument, pass it to the
physician by the handles or carefully put it into sterile jar to be offered
later [don’t contaminate the cassette cover or instrument’s tips; close the
cassette as soon as possible],
- cut the sterile package of gauze swabs with bandage scissors (usually
paper wrapper), open the package carefully [don’t touch the inside of the
wrapper], draw the swab by handling forceps and pass it to the physician
to be carefully caught by the physician’s forceps [avoid touching the
instruments],
- open the sterile cleaning solution and pour it over the sterile swab held by
the physician’s forceps to moisten the swab [be aware not to touch the
swab keeping the bottle lip approx. 10 cm above the swab; physician
should hold the swab approx. 10-15 cm over the kidney dish as well],
- when ordered, prepare the irrigation delivery system by filling the sterile
syringe with irrigation solution and/or attaching the sterile catheter,
18. Cleanse the wound by mechanical cleaning or irrigation
- if indicated to take a specimen from the wound, always obtain it before
cleansing it,
- clean the wound using the forceps and gauze swabs moistened by cleaning
solution following the principles of wound cleansing [keep the forceps
tips lower than the handles all the time, use separate swab for each
stroke, discard each swab used],
- if a drain is present, clean it after the incision [the main surgical incision
is considered cleaner because of considerable drainage around the
drain], support and hold the drain erect while cleaning around it,
- if irrigating wound, flush it using slow continuous pressure, ensuring
gravitational flow of irrigating solution through the wound into this
collection basin,
- dry the surrounding skin with dry gauze swabs as required, if needed
clean up the skin from tape marks with some solvent.
19. Apply powders / ointments as ordered
- shake powders directly onto a wound / use sterile applicators to apply
ointments [sterile tongue blades / sterile blunt instruments such as
forceps, surgical probe]
20. Apply dressings to the drain site and the incision
- apply all the layers of dressings as needed [primary layer is usually
applied moistened with cleaning solution, secondary is usually dry or
absorbent if needed; manipulate with forceps using surgical aseptic
technique; if necessary, use forceps to feed the gauze square gradually
into deep depressed wound areas – undermining, tunneling],
- if a drain is present, place a pre-cut sterile gauze square [or cut the sterile
square with sterile surgical scissors] nearly around the drain,
- secure the dressings as needed.
21. Remove PPE, assist the patient into a comfortable position, dispose
of equipment and soiled supplies and perform thoroughly hand hygiene.
22. Inspect the wound dressing periodically to determine patient’s
response to wound care procedure (at least every shift).
Complications
- wound dressing is dry and too adherent when removed,
- appearance of bleeding or serosanguineous drainage, retained fluid or
debris, increased pain or discomfort in the patient, signs of inflammation,
- increase of wound drainage (more than dressing can absorb),
- irritation of the skin around the wound (red, macerated or excoriated),
- suture line opening extends or a drain is removed accidentally,
- dressing doesn’t stay in place.
Documentation
- record the procedure performed (e.g. type of solution used, type and
amount of dressings applied, frequency of dressing change) including
wound assessment before and after the procedure, patient’s tolerance of
the procedure, patient’s status prior to and its evaluation during and after
the procedure,
- immediately report to the physician (if he or she isn’t present) any
evidence of fresh bleeding, sharp increase in pain, accidental removal of a
drain, evidence of wound dehiscence or evisceration (particularly in the
wounds after removal of sutures), signs of shock,
- record and report possible allergy reaction – the cause, patient’s response,
vital signs, treatment applied and reaction to it.
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