Jerrone Hanson

Block 4
Ms. Russo
Enzyme Catalysis Lab report

Enzyme Activity

Activity Observation
This shows what happens
to the enzyme at certain
Enzyme Activity Bubbles.
states or when it is
Effects when tempreture exposedHappens
Nothing to certainbecause it
rises environments or even temp.
denatures at extreme
When The catalysis is when it comes in contact
It Bubbles.
present with certain things.

The Baseline

Volume
This is the Catalyzed
Decomposition
Initial Reading 5ml
measurements.
Final Reading 2.4ml
Baseline (which is the initial 2.6ml
volume minus the final
volume)

Class Data

Time Amount of H2O2 of Amount

The Collection the classof H2O2 Average
Decomposed in Decomposed in Amount of
data which shows
Trial 1 Trial 2 H2O2
different seconds and Decompose
measurements of the d
10 experiment
1.43 that differs1.03 1.23
Seconds depending on the
30 2.23 of the lab. The.83
accuracy 1.53
Seconds trial two(2) of the 30
60 2.03 reaction was 1.83
Second 1.93
Seconds
reasonably low because
120 2.63 1.63 2.13
Seconds of some error that might
180 have
2.83happened in the 2.43 2.63
Seconds experiment, such as the
enzyme might have been
weaker than usual, But
overall, but observing the
average amount that was
Class Data2 (Graph)

Amount of Hydrogen Peroxide Decomposed There were two trials

(Trial 1 & 2) used for each time
ranging from ten (10)
seconds to one hundred
and eighty (180)
seconds. Notice that the
amount of H2O2
decomposed increases
with time, except for trial
two (2) at thirty (30)
seconds which the
amount fell the lowest
and this could be caused
by errors in terms of

Class Average data (Graph)

By observing the
outcome of the class’s
average data, it made
sense that as the time
increased, the amount of
hydrogen peroxide also
increased. The rate was
not totally consistent, but
it never fell, it was
always increasing as
time increases.

Analysis

The purpose of this lab was to observe and understand the effects of
changes in temperature, pH, enzyme concentration, and substrate
concentration on the reaction rate of an enzyme-catalyzed reaction. Another
purpose of the lab was to explain how environmental factors affect the rate
of enzyme-catalyzed reactions. The data and graph shows that during the
180 seconds, the rate of reaction was the fastest, which means that more
Hydrogen peroxide was decomposed at in the shortest amount of time.
Conclusion

The catalase enzyme helped in the reaction of decomposing hydrogen

peroxide into water and oxygen. However, enzymes are very
environmentally sensitive, so our group was able to see direct effects on the
reaction when the catalase solution was out for a period of time and possibly,
denaturing it and reducing the results of the reaction. As for the effect of
time and the catalase on the rate of reaction, we were able to see a high rate
of reaction toward the beginning of the reaction, but it slowly leveled out
when more of the H2O2 had decomposed. This experiment could be
improved by trying to be more accurate when measuring the reactants
before they are added to the solution. Perhaps if the catalase was not out for
as long, the experiment could have given a better result.