A wavelength of 510 nm corresponds to an

approximate frequency of 6*1014 1/sec. What is

the approximate energy of 1 photon of this light?

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20 Agenda:

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M : Beer Law
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 Beer's Law and Spectrophotometry:
Beer-Lambert Law gives the relationship between the absorbance of
light in a sample and its concentration. The path length and the molar
absorptivity are held constant (based on the instrument and the
wavelength of light used).

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Beer's Law and Spectrophotometry:
When deciding to do spectrophotometry the first thing one must do is
select the correct wavelength of light to do the experiment at.
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The correct wavelength is the one that has MAXIMUM

absorbance in a certain material. If you plot absorbance vs
wavelength for a given concentration you get a graph like below.

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It's worth noting that colors come from reflecting light back to your
eyes- so something that looks blue will MOST absorb in the orange/
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red area of the spectrum and reflect the most blue. This can help you
-

better predict where this should occur.

 In a spectrophotometry experiment...
O 1. A calibration curve is created using known concentrations of

El . a particular ion at a fixed wavelength and optical length which

creates a plot of absorbance vs concentration.
-

2. An unknown with the particular ion is analyzed at the same

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wavelength and is compared to the curve. Based on where it

lands on the graph- you can tell its concentration.

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x-axis = molarity
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slope = (molar absorptivity * cuvette width (typically 1cm))
Let's walk through an experiment:
Goal: Identify the concentration of dichromate ion in an unknown
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Part 1: Selecting a wavelength.

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Part 2: Building the Calibration✓
Curve
1. We need to make 25.00 mL 0.2 M solution of K2Cr2O7. How
many grams would this require (molar mass 294.19 g/mol)?

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2. From this we will perform a dilution in order to create 0.15 M,
and 0.1 M solutions each with a volume of 10.00 mL. How
much from each solution will we need to do this?
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An unknown sample contains a total mass of 0.98 grams. You
dissolve the sample into acid and then analyze it. You find that it has
an absorption of 0.73

4.5024×+0.0419
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0.73--4.5024×+0.0419

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a. What is concentration of Cr2O72- in this sample?
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b. What is the mass % of K2Cr2O7

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