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A critical appreciation/review of
“Population Genetic Structure in Indian Austroasiatic Speakers: The Role of
Landscape Barriers and Sex-Specific Admixture,
by
Gyaneshwer Chaubey, Mait Metspalu, Ying Choi, Reedik Magi, Irene Gallego Romero, Pedro Soares, Mannis
van Oven, Doron M. Behar, Siiri Rootsi, Georgi Hudjashov, Chandana Basu Mallick, Monika Karmin, Mari Nelis,
Ju¨ri Parik, Alla Goverdhana Reddy, Ene Metspalu, George van Driem, Yali Xue, Chris Tyler-Smith, Kumarasamy
Thangaraj, Lalji Singh, Maido Remm, Martin B. Richards, Marta Mirazon Lahr, Manfred Kayser, Richard Villems,
and Toomas Kivisild, Mol. Biol. Evol. 28(2):1013–1024. 2011 doi:10.1093/molbev/msq288”
When HUGO Pan-Asian SNP Consortium published results of its very large
study of autosomal SNPs involving 73 populations living in ten Asian
countries, it became clear that it had sorted out the question of human
migration in the east. 1 They found that East Asian populations originated from
Southeast Asia, and that Myanmar, Borneo, Thailand and Cambodia were
important as a source of human population to China. It further clarified that
Southeast Asian population had in turn originated from India. Five percent of
East Asian population had originated from India directly, implying an exit out
of India from Tibet-Burma side directly into China. They also noted overlap
of DNA migrations and language. From the conclusions of this study, it
became evident without saying that the Austro-Asiatic, Daic and
Austronesian speakers of Southeast Asia had originated genetically, if not
linguistically, from India and that the claim of their origin from China was
false.
1
The HUGO Pan-Asian SNP Consortium, Mapping Human Genetic Diversity in Asia,
Science 2009, 326(5959): 1514-1545.
2
than HUGO’s. Yet Bellwood and Higham pervaded over the authors of the
study done by Chaubey et al.
This China hypothesis had stated that farming reached Taiwan from China,
and then there was a Taiwanese population expansion leading to
Austronesian expansion, which led to founder populations settling in
Indonesia, Malagasy and Pacific Islands. This theory too got discredited by
DNA studies soon. Without verifying the validity of Bellwood Hypothesis,
many authors (working with human DNA) believed words of Bellwood. For
example, Hill et al (2006) write, “Both Austroasiatic and Austronesian
languages had their origins in South China and were introduced to Southeast
Asia during the middle Holocene with the Neolithic expansion of farmers of
Mongoloid physical type. Austroasiatic took a mainland route southwards,
whereas Austronesian expanded along the island arc from Taiwan to the
Philippines, and then Indonesia and Malaysia.” 2 Identifying a particular body
type (Mongoloid in this case) with such historical events was outright racism,
which has not been supported by any DNA studies.
The monogenic theory of linguistics, 3 which is not yet widely accepted and
known, is consistent with monogenic DNA based theory of origin of Homo
sapiens sapiens, and should have long back replaced the main-stream racist
linguistics which believes multiple origins of languages in different isolated
races.
2
Hill, C. et al, Phylogeography and Ethnogenesis of Aboriginal Southeast Asians, Mol Biol
Evol 2006, 23(12): 2480-2491.
3
Bengston, J. D. and Ruhlen, M., “Global Etymologies”, in On the Origin of Languages: Studies
in Linguistic Taxonomy, Stanford University Press, Stanford. Pp. 277-336.
3
At the very outset the discussion opens with a premise: “The hypothesis that
the spread of the Austroasiatic language family can be traced back to rice
cultivators of Southeast Asia (Higham 20034; Bellwood 20055) is contested,
but some relationship between early Austroasiatics and rice agriculture is a
view that remains prevalent among linguists.” (p. 1014). The authors’
inclination to this hypothesis is clear in the quote, although the expressed
intention is to verify this hypothesis.
4
Higham, C., “Languages and farming dispersals: austroasiatic languages and rice
cultivation”, in Bellwood, P. and Renfrew, C., (Eds.), Examining the farming/language
dispersal hypothesis, The McDonald Institute for Archaeological Research, Cambridge, p.
223–233.
5
Bellwood, P. S., First farmers, Wiley-Blackwell, London, 2005.
6
Priyadarshi, P., The First Civilization of the World, Siddhartha Publications, New Delhi,
2011. -----, Recent Studies in Indian Archaeo-linguistics and Archaeo-genetics having bearing on Indian
Prehistory, Joint Annual Conference of Indian Archaeology Society, Indian Society for
Prehistoric and Quaternary Studies, Indian History and Culture Society, Lucknow, 30
December, 2010
4
The authors would have spent their time more fruitfully if they had studied
works in DNA of rice, cattle, commensals and humans which have thoroughly
ruled out these possibilities. Before we proceed to critically examine the
article by Chaubey et al (2011), it is desirable to list the selected important
works which the authors have been oblivious to consult. We can just mention
only a few of those works here:
Human Migration Took Place from Southeast Asia to China and not the Vice
Versa during the Period Under Consideration:
(This rules out theory of Chinese migration into SEA with rice agriculture)
Autosomal DNA studies too point out that the Austro-Asiatic speakers
are autochthonous to India and the Southeast Asians originated from
India
1. Oka, H. and Morishima, H., “Wild and cultivated rice”, in Matsuo, T.,
Futsuhara, Y., Kikushi, F. and Yamaguchi, H. (eds), Science of the rice
plant, vol 3: Genetics, Nobunkyo, Tokyo, 1997, pp 88–111.
2. Glaszmann, J.C., Isozymes and classification of Asian rice varieties,
Theor Appl Genet 1987, 74:21–30.
3. Wang, Z.Y. and Tanksley, S.D., Restriction fragment length
polymorphism in Oryza sativa L., Genome 1989, 32:1113–1118.
4. Blair, M.W., Panaud, O. and McCouch, S.R., Inter-simple sequence
repeat (ISSR) amplification for analysis of microsatellite motif
frequency and fingerprinting in rice ( Oryza sativa L.), Theor Appl Genet
1999, 98:780–782.
5. Cheng, C. et al, Polyphyletic origin of cultivated rice: based on the
interspersion pattern of SINEs, Mol Biol Evol 2003, 20:67–75.
6. Vitte, C. et al, Genomic paleontology provides evidence for two distinct
origins of Asian rice (Oryza sativa L.), Mol Gen Genomics 2004,
272:504-511.
7. Kovach, M. et al, New Insights into history of rice domestication,
Trends in Genetics 2007, 23(11): 278-287.
8. Yamane, Hiroko et al, Molecular and Evolutionary analysis of the Hd6
Photoperiod Sensitivity Gene Within Genus Oryza, Rice 2009, 2:56-66.
9. Hiroko, Y. et al, Molecular and Evolutionary analysis of the Hd6
Photoperiod Sensitivity Gene Within Genus Oryza, Rice 2009, 2:56-66.
10. Lonedo J. P. et al, Phylogenography of Asian wild rice, Oryza rufipogen,
reveals multiple independent domestications of cultivated rice oryza
sativa, PNAS 2006, 103, 9578-5983. Chen, et al, Distribution of
deletion type in CpDNA of cultivated and wild rice, in Japanese Journal
of Genetics 2003, 68: 597-603.
11. Harris, David, “The Multi-disciplinary Study of Agricultural Origins:
‘One World Archeology’ in Practice”, in The Future for Archeology,
edited by Layton, Robert et al, Routledge Cavendish, 2006, p. 238.
Wild Ancestors of Chinese Rice are mainly found in India and Southeast Asia
Japonica Rice (Chinese Rice) did not originate in South China, but originated
in the Island Southeast Asia where it was domesticated too: Chinese rice
domestication is a myth
7
This is consistent with the human DNA finding that Austronesian speakers
from ISEA entered and spread their culture into the Mainland Southeast Asia:
Peng, Min-Sheng et al, Tracing the Austronesian Footprint in Mainland
Southeast Asia: A Perspective from Mitochondrial DNA, Mol. Biol. Evol. 2010,
27(10):2417–2430.
Genes Crucial for domestication originated in Indian rice and migrated later to
China, then entered Japonica rice (by cross pollination)
1. Murphy, D.J., People, Plants and Genes: Story of Crops and Humanity ,
Oxford University Press, 2007 (p. 178).
2. Edmondson, Jerry, The power of language over the past: Tai settlement
and Tai linguistics in southern China and northern Vietnam,:
http://ling.uta.edu/~jerry/pol.pdf (Edmondson is a Utah University
professor of linguistics and specialist of SEA).
8
Mice and Rats migrated from India to Southeast Asia during the period under
consideration
7
http://www.scribd.com/doc/44092576/Origin-of-Indo-European-languages-and-farming-
Evidence-from-Human-Animal-and-plant-DNAs-and-from-linguistics pp. 6-8.
9
http://www.springerlink.com/content/v12217m867085l44/fulltext.html#
Fig1_1562
3. Geraldis, Armando, et al, Inferring the history of speciation in house
mice from autosomal, X-linked, Y-linked and mitochondrial genes,
Molecular Ecology 2008, 17(24):5349-5363.
4. Din, W. et al, Origin and radiation of the house mouse: clues from
nuclear genes, Journal of Evolutionary Biology 1996, 9(5):519-539.
5. Boursot, P. et al, Evolution of House Mice, Annual Review of
Ecology and Systematics 1993, 24:119-152.
[Linguistically too-- Thai (koh), Pinyin Chinese (gu and ku), Cantonese ngau
all meaning ‘cow’ are only modified forms of Indo-European word ‘cow’ and
Sanskrit gau].
***
Thus we can see that the authors of this article missed to read the valuable
wealth of knowledge gathered from DNA studies of rice, cattle, buffalo, mice
and rats as well as archaeological studies pertaining to them. There are other
gross problems as well. Their conclusion and results are conflicting with the
works done earlier on this same topic:
Apart from not exhausting the published literature on the topic, there are
many other problems in the article. For example just to cite a few:
This has resulted primarily because of not understanding the nature of +/-
range of statistics. When a range of this type is given in statistics, like 23.8
+/- 4.2 kya, it is equally well possible that the Mainland SEA had this DNA at
the earliest about 19,600 years back, and the Indian one (South Munda, 18.4
+/- 2.4 kya) had it 20,800 years back. Under such circumstances, it is the
ancillary evidence which becomes important, which in this case will be:
studies of rice, cow, mice and buffalo DNAs. Other ancillary evidence would
be studies of mitochondrial DNA or autosomal DNA.
Chaubey et al must have appreciated that a group of only one male lineage
O2a (M95) could not have migrated in isolation without incorporating some
non-O2a males belonging to their society. Thus any migration from Southeast
Asia to Central and East India must have carried some O3 and O1a male
lineages along with the O2a lineage. But we do not find any presence of such
eastern lineages in the Munda speakers. On the other hand, the Southeast
Asians carry many male Y-chromosomal lineages (haplogroups) which are
provenly Indian in origin, and which are evidence that they went to SEA along
with O2a. Some such Y-chromosomal haplogroups are F, H, K2 (T) etc.
Moreover the men carrying O2a must have been accompanied by some
women. These lineages—both male and female—should be reflected in the
story of migration. An earlier work found that the mitochondrial DNA lineage
R7, which had been alleged to be Southeast Asian, and also allegedly had
migrated to India with Austro-Asiatic speakers, was in fact Indian in origin
(Chaubey, G. et al; “Phylogeography of mtDNA haplogroup R7 in the Indian
peninsula”, in BMC Evol. Biol. 2008 Aug 4, 8:227). This clearly proved that
the mtDNA R7 originated in India and migrated with O2a and other males, who
spoke Austro-Asiatic languages, to the Southeast Asia.
Many more such works on mitochondrial DNAs have proved that the Austro-
Asiatic speakers are autochthonous population of India, and migration if at all
occurred, must have been from India to SEA:
1. Edwin, Deepa et al; “Mitochondrial DNA diversity among five tribal
populations of southern India”, in Research Communications of Current
Science, July 2002, 83(2): p. 158.
2. Mountain J. L. et al, “Demographic history of India and mtDNA-
sequence diversity”, in Am J Hum Genet 1995, 56 (4):979-992;
3. Majumder, P. P.; “Ethnic population of India as seen from an
evolutionary perspective”, in J. Biosci 2001, 26 (4 suppl): 533-545;
4. Sharma, S. et al; “The Indian origin of paternal haplogroup R1a1*
substantiates the autochthonous origin of Brahmins and the caste
system”, in Journal of Human Genetics 2009, 54: 47–55.
5. Chaubey, G. et al; “Phylogeography of mtDNA haplogroup R7 in the
Indian peninsula”, in BMC Evol. Biol. 2008 Aug 4, 8:227.
12
Associating the racial feature of straight thick hairs with the East Asians, and
presuming that racial features or races have evolved separately in different
areas of the world is a product of racist unconscious of the authors. Thus
they studied one autosomal “EDAR 1540C” gene which causes thick hairs.
They presumed that this is a Mongoloid feature and must have originated in
the Mongoloid homeland East Asia.
Far from being that, this mutation or gene (EDAR 1540 C) as well as most of
such racial markers originated in the common gene pool before dispersal to
East. This particular hair gene originated in India after the European lineages
had left India.8 Yet it has spread to west of India too in low frequencies,
because of later low grade migration out of India to West. This is clear from
the EDAR 1540C distribution map drawn in the article (Fig. 4A, page 1020),
which shows that the gene is found in low frequencies in North and East
Africa, West Europe, India and in high frequency in both the Americas and
China. During Last Glacial Maximum it was selected favourably in colder
areas of world, because it provided protection to the head from cold (ibid).
Thus its frequency increased in all those populations which were exposed to
cold. This same gene is also associated with shovel shaped incisors9, which is
so common in India especially in Bihar, Bengal and Orissa.
Chaubey et al’s data depicted in Fig. 4B (p. 1020) shows that this gene (EDAR
1540) is significant in all those populations of India/ South Asia which are
considered very old inhabitants of the sub-continent because of presence of
old Y-chromosomal as well as mitochondrial DNA markers. Thus Hazra
(Afghanistan), Burusho (Kashmir, India) and Tharu10 (Indo-European speaking
Indo-Nepal boarder region) were found to have this gene by Chaubey et al.
These were considered migrants from East Asia on this account by the wise
authors.
Thus Chaubey et al (2011) propose that the Tharu arrived into India from
Southeast Asia, when they spoke Austro-Asiatic language, but they later
converted to Indo-European. Fornarino et al showed that Tharu men carry Y-
8
Fujimoto, A. et al, A scan for genetic determinants of human hair morphology: EDAR is
associated with Asian hair thickness, Human Molecular Genetics, 2008, 17(6): 835–843.
9
Kimura, R. et al, A Common Variation in EDAR Is a Genetic Determinant of Shovel-
Shaped Incisors, AJHG 2009, 85(4): 528-535
10
Fornarino, S. et al, Mitochondrial and Y-chromosome diversity of the Tharus (Nepal): a
reservoir of genetic variation, BMC Evolutionary Biology 2009, 9:154.
13
chromosomal lineages of C5, D*, D1, E1b1b, H1a1*, H2, J2a*, J2b2*, K*, L1,
O2a1a1, O3a3c*, O3a3c1, O1, R1a1* and R2 haplogroups. There was no O2a*
or an early branch of O2a in the Tharu population. Hence such suggestion by
the learned authors that Tharu belonged to Austro-Asiatic stock is
unfortunate.
The article also reflects obvious bias and manipulation of data. The authors
have not included any Indo-European O2a or Dravidian speaking O2a sample
from India deliberately. On the other hand in case of Southeast Asia, they
have included O2a samples from Mainland Southeast Asia, China, Taiwan and
Island Southeast Asia. These samples have included individuals speaking not
only Austro-Asiatic languages, but also Daic, Chinese and Austronesian
languages. This has increased the variability and hence age of the so called
Southeast Asian sample.
Moreover, the dates arrived at for migration into India (18,600 years back) is
too early to be associated with farming if we should believe Bellwood.
Thus a reader of this article may get misled by the conclusions of this article,
which is no more than personal preferred belief of some of the most powerful
authors in this field of human migration.