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1.1As per policy of the University and clinical biochemistry lab that work, whether associated
with teaching or research, be done efficiently and safely.

1.2 Laboratory personnel have an obligation to protect themselves and others from hazards
resulting from their acts or negligence in the laboratory.

1.3 Only the concerned persons are permitted to be in the laboratory. Visitors or unconcerned
should be asked to leave.

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2.1. Smoking, eating and drinking are not permitted in the laboratory.

2.2. Outdoor clothing must be left in a cloakroom. Bags and other objects must be placed on side
and not allowed to obstruct pathways or bench tops.

2.3. Suitable laboratory coats must be worn in the laboratory and removed after leaving. Safety
goggles must be worn when carrying out any procedure where may be slightest risk of eye
injury; gloves of the appropriate type must be worn when necessary. Long hair must be
restrained e.g. by means of a cap, ribbon or headband.

2.4. The information about usage of equipment and the handling of potentially hazardous
materials should be obtained in advance. If anyone, for some reason, failed to get appropriate
knowledge about the equipment, then it is his or her responsibility to bring the lack of
knowledge to the attention of the Chief Technician, so that appropriate arrangements can be
made to remedy the situation.

2.6. Glassware and plastic ware that is being used should be labeled with felt-tip pen; this avoids
confusion and will help the laboratory staff.

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3.1. Where a potential hazard exists in a particular test, this will be discussed in the talk before
running the test and details of safe working methods will be highlighted in the notes.
 3.2. Trainees must NOT use unfamiliar equipment or procedures without them having been given
instruction. All safety instructions given in the preliminary talk and practical notes must be
adhered to.

3.3. Mouth pipetting is forbidden.

3.4. All hazardous materials are placed in a dedicated area for this purpose.

3.5. Never use toxic substances without taking the proper precautions and making arrangements
for safe working. Use volatile solvents in a fume hood.

3.6. Broken glassware must be placed in designated sharp containers(special bins provided in the
laboratory).

.7. Used plastic pipette tips must be placed in the appropriate labeled container on the bench.

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4.1. Bench should be left waste-free and tidy at the end of each session ± this reduces potential
for accidents and spillages and is of considerable help to the laboratory staff.

4.1. Laboratory equipment must be left clean after work.

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5.1. All accidents and spillages, including any personal injuries and damage caused to equipment,
must be reported as soon as practicable to the supervisor, the chief technician or other
colleagues.

5.2. Concentrated acid or alkali on the skin:

a) flood the splashed surface thoroughly with water and continue until satisfied that no
chemical remains in contact with the skin. Soap will help to remove chemical which are
insoluble in water;
b) remove all contaminated clothing take care not to contaminate yourself in the process.

5.3. Splashes in the eye.

Eye protection should be worn for any work where there is a potential hazard but if accident
occurs:
a) flood the eye thoroughly but gently with water;
b) seek medical advice for all eye injuries from chemicals.

5.4. Burns and scalds. Cool affected area by immersing in cold water. Seed is essential. Continue
for at least 5 minutes or until pain is relieved.

5.5. Spillages must be cleared up immediately and the area decontaminated; they must NOT be
left as a hazard to others.
 5.6. A first aid box is located in the Laboratory. Consult supervisor if you need items from the
first aid box.

The diagram identifies three color-coded categories of hazard for each

material:

Health hazard ($

Flammability ($),
Reactivity (%$), and
Other hazard information (white quadrant ).
Each category is divided in five levels of hazard potential with
&'() used to indicate no special hazards and
'Πfor severe or extreme hazard potential.

The degrees of hazard in each of these categories are given as follows:

 ·
 The degree of health hazard of a chemical or material is based on the form or
condition of the material, as well as its inherent properties'NFPA ratings).. The
degree of health hazard of a material should indicate the degree of personal
protective equipment required for working safety with the material:
 A rating of 1: slightly hazardous (toxic) materialwhich require only minimal protection (for
example, safety glasses and gloves) in addition to normal work clothing to work with
safely.

 A rating of 2: moderately toxic or hazardous materialwhich require additional PPE or

equipment (e.g. chemical goggles, lab/work smock, local ventilation) in addition to that
 required for less toxic material. Consult the MSDS for specific health hazard
and proper PPE to use with this material.

 A rating of 3 or 4: highly to extremely toxic (deadly) material (and any carcinogen,

mutagen, or teratogen). You must consult the MSDS and/or other safety information to
determine the hazard (acute or chronic) and the proper PPE and engineering controls to
safely use of this material.

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Ñ The flammability hazards deal with the degree of susceptibility of the
material to ignite and burn. The form or condition of the materials, as well as their
properties, affects the extent of the hazard.
Rating is according to ignition temperature of the materials.
 
  
Ñ The reactivity hazards deal with the potential of a material or chemical to release
energy. Some materials are capable of rapid release of energy without any
catalyst, while others can undergo violent eruptive or explosive reactions if they
come in contact with water or other materials.
A low rating of indicates a )* . The
more hazardous ratings of ï

Πindicate a *)

 +, -)
PASS
To use a fire extinguisher, remember the acronym c . This stands for

c.// the pin

0 the hose at the base of the fire
1.& the lever
"c the hose from side to side.
RACE




 
 




 





 
 
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RESCUE:

 
 




ALARM:
  









CONTAIN:
 





 




 




EXTINGUISH

EVACUATE:









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EMERGENCY
Know what to do.
Know where things are:
The nearest fire extinguisher,
fire alarm box, exit(s),
telephone, emergency shower/eyewash, and first aid kit, etc.

£/2!

Examples of common hazards include the following:

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Toxins, corrosives, flammables, and reactives

  




Microbes, animals, plants, and genetically modified agents



 



Ionizing and nonionizing radiation


 




Heating devices, noise, projectiles, fire, cold, etc.

  




Fire and shock

 
 




Moving machinery

  


 


Vapors, dust, etc.