Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
: 1 Date: ______________
Signature
Loris
Round-head with large and round eyes
Stout in body and limb with a coat of dense fur
Head and shoulders may be silvery white, creamy or ash grey. Brown stripe is very distinctive
marking the middle line of its back and terminating on the crown
Distinct brown circles around it‟s lustrous and owl-like eyes
Two species are there
Signature
HOUSE CROW
Neck is grey colored one
Sound is unique one
Effective scavengers
Lives in close association with man
JUNGLE CROWS
Body is jet black unlike the house crows
Deep and hoarse sound is made by these birds
Heavier than the house crows
Sexes are alike
Associates with vultures to feed on carrion
RAVEN
Larger and heavier birds
Larger than the jungle crows
INDIAN MYNAH
Dark brown bird with the bright yellow bill and legs
Bare skin is seen around the eyes
HILL MYNAH
They are seen as noisy-flocks in hill forests
Jet black color is seen on the body
Conspicuous white patch is seen on the wings
Yellow bill and legs are present
SPARROW
This is the most familiar bird
Body is grey colored
Lives in human association
BLACK DRONGO
This is also called as king crow. Found through-out India
Perched on telephone wires
Commonly seen in previously-fire exposed areas
Beneficial to farmers in general
EMERALD DOVE
Bronze winged dove is the other name for this
Brownish pink dove with bronze green color in upper areas
INDIAN CUCKOO
Slaty grey above white regions are seen below and is barred with black
Black tipped tail is diagnostic
WHITE IBIS
Head, neck and long curved bill are black colored ones
However body is white
EGRETS
Large egret
Bill is black and yellow or black
Lives in marshes and river-areas
Cattle egret
These are seen in water bodies
Yellow bill is unique in nature unlike the little egret
Little egret
Smaller than the cattle egrets
Bill is black coloured one
HERONS
Pond heron
These birds are also called as Paddy birds
Earthly brown at rest
White colored wing, tail and rump are present
Night heron
These are grey and black stocky birds
Black colored crest is seen
PAINTED STORK
These are the only white storks with delicate rose-pink on shoulder and wing regions
SARUS CRANE
Tall bird
Naked red head and upper neck are characteristic in these birds
Flamingo
These are the long necked rosy white birds
Heavy and curved bill that is pink coloured one
Black bordered scarlet-wings are present
PARAKEET
Rose ringed parakeet
Rose pink collar is present in case of male birds
In females, such collar is absent
Alexandrine parakeet
Called as large Indian parakeet
Conspicuous maroon patch on each shoulder
BABBLER
These birds are larger than the bulbul
Color is of earthy brown with darkly streaked upper plumage
Seen always in flocks of a six or more on ground
Some times, they are seen on bushes that are low-lying ones
HOOPOE
These birds have conspicuous fan-shaped crest
Gently curved bill
MALABAR HORNBILL
Seen in South India frequently
Black and white colored body
Massive yellow and black bill with a casque ending in a single point
DARTER
These birds have long neck which is slender and snake like one
Pointed knife like bill is present
CORMORANT
Little cormorant
Have glistening black colour
Long strong tail
Small white-colored patch is seen on the throat region
HUMMING BIRD
Long bill and extensible tongue are suited to reach the nectar in flowers
Able to hover on a spot
Capable of rapidly beating the wings ( 22-78 beats per second)
National bird of Jamaica
PARADISE FLYCATCHER
Ribbon like feathers in tail is a characteristic feature
Silver-white in colour
PEACOCK
Crest is seen in both sexes
Males are having train of feathers unlike the females (peafowls)
Sound is a characteristic one
Seen in moist dry-deciduous forests and villages
BENGAL VULTURE
This is also called as white backed vulture
White coloured back is present
White band is present on underside of wings and this is characteristic
BLACK EAGLE
Seen in South India
White patch is seen under eye
Tail is black with grey-barred
PARIAH KITE
Forked tail is characteristic of these birds
Brown in colour
Bright rutsty red colour is seen in upper areas and white colored areas are seen in other regions
especially in anterior quarters
PEREGRINE FALCON
Aereal predator
Feeds on smaller sized mammals especially rodents, birds etc.
Slaty black coloured head and black coloured cheek are present
Black intermittent stripes are present in white ventral body regions
BARN OWL
Also called as screech owls
Large round head
Monkey like facial-appearance
Sexes are alike and may be seen singly or in pairs
SHIKRA
Bluish grey color is seen on upper areas in case of male birds, along with white barred brown
color in lower areas
In case of females, brownish color is seen in upper areas
OSTRICH
Belongs to Ratites group
Largest of living birds
Seen in African plains
Weight is about 160 Kg
EMU
Belongs to Ratites group
Seen in Australia
Weight assumed is often 68 Kg
CASSOWARY
Belongs to Ratites group
Beak is a characteristic one and is heavy
Large powerful birds
Seen in northern Australia, New Guinea and adjacent islands
RHEAS
Belongs to Ratites group
These birds are from South America
Wings are well developed for courtship and aggressive activities and display
Signature
Practical Manual VMD-512 16
Practical No.: 4 Date: ______________
SERPENTINES
It is to be remembered that nearly one third of snakes in the world are poisonous in nature to
some degree. Snakes are the most recently evolved reptiles and hence may have more features that are
in common with lizards. Of the 244 species of Indian snakes, 52 are poisonous in nature.
BLACK COBRA
Scales are barred
Poisonous snake and is seen in extreme north-west India
KING COBRA
Large sized snake
Colour of body varies from blackish brown to light brown
Smooth and shiny scales
Distinct light cross bands mainly on the fore body
Large head scales are found edged with black
Most poisonous snake and is diurnal
Average length: 3 mt and at birth it is about 50 cm
RUSSELLS' VIPER
Medium to large sized snakes
Strongly keeled snakes and thick body is present
Sensory pits are present between eye and nostril
Distinctive bright chain pattern
Large triangular head
Poisonous snake
Average length: 1 mt and at birth it is about 24 cm
SAW-SCALED VIPER
Small sized snake
Strongly keeled snakes
Head is wider than the neck
Dull color
Cross mark on the top of head is distinctive
Poisonous snake
Average length: 30 cm and at birth it is about 8 cm
SEA SNAKE
Mostly poisonous in nature
Tail is paddle like and is laterally compressed
Color is olive green above with yellowish bars
RAT SNAKE
Largest snake
Commonly seen in agricultural fields and human habitations
Non-poisonous snake
CROCODILIA
The members of this order are amphibious in nature. The skin of crocodiles consists of leathery
and tough scales joined at their margins by thinner and more pliable skin. The dorsum has the ridged
or keeled scales but the belly are smooth. Scales may overlap and beneath the dorsal epidermal scales
are dermal ossifications that stiffen the body covering. Head is flattened and is adapted for the floating
on water. Musk glands are present on the medial side of each mandible and on either side of the cloaca
close to the vent. Tail is used as a repellant.
Practical Manual VMD-512 18
MUGGER CROCODILES
Also called as „Marsh crocodile‟
Seen through out India
Large sized one
Head is broad
GHARIALS
Also called as „Ghavials‟
Snout is longer and is narrow
In males, the tip of nostrils is swollen as a bulbous „pot‟
AMERICAN ALLIGATORS
In general, the alligators are not found in India but are kept in various zoos
They are from south-east USA
CAYMAN
These are the members of alligator family
These may grow up to one meter in length
Chelonians
This group comprises turtles, tortoises and terrapins. Turtles are the most ancient living
reptiles. Sea turtles are largest ones and females come to shore only to lay eggs. Variable sized
chelonians are available in general.
o Terrapins are the fresh water dwelling species
o Tortoises can also be called as „land turtles‟
Turtles Tortoises
Flippers are present Not present
Mostly sea living ones Land living
Skin is smooth Skin is thickest with large tuberculate scales
ALDABRA TORTOISE
Largest one. Weight of this is about 250 Kg
LIZARDS
Some lizards have lost their limbs in the evolution processes, like retaining of part of the hind
limbs by some snakes. Lizards are most widely distributed and are able to adapt to wide range of
habitats. Periodical renewing of skin occurs like snakes. The lizards differ from snakes in having
eyelids and an obvious ear opening. This comprises house geckos, garden lizards, chameleons,
Iguanas, monitor lizards etc. Growth of lizards is slow. Large geckos, monitors, iguanas live for about
10-20 years.
HOUSE GECKOS
These are commonly seen in houses, alert and active insectivorous lizards
These have adhesive pads and claws
Sexes look alike
Autotomy is a characteristic feature noticed
CHAMELEONS
These are capable of changing the body colors frequently and this is possible because of the
presence of chromatophores in the skin areas
Arboreal ones. Tail is a characteristic feature and is prehensile in nature
Tongue is highly protrusible in nature
IGUANA
These are the lengthy lizards and have specialized body requirements
Long tongue is present which is forked in nature
KOMODO DRAGON
This is also called as “Komodo lizard” or “Ora”
Body has a covering of small scales
Stocky in appearance
They are seen in Indonesian islands and Komodo
Weight is about 140 Kg and is 3 mt length, in general
Occasionally it attacks and kills man
Signature
Practical Manual VMD-512 20
Practical No.: 5 Date: ______________
OBJECTIVES
1. To understand on significance of pug mark-model identification
2. Identification of pug mark by drawing the diagrams of different pug marks and seeing the live
pug mark-models made
3. Document the pug mark by glass tracer method and casting techniques
MATERIALS REQUIRED
Pug mark design-models of different wild animal species
Glass material
Permanent marker pens
Plaster of Paris
Wax material
Heating source
PRINCIPLE ASSOCIATED
Pug mark identification is necessary because of the simple fact that in general no wild animal
may stand in one place for a long time to get sighted. This is much true in case of carnivores
which may be always seen running or hiding in most of the periods.
Hence, animal census needs to be taken by some indirect measures like identification of pug
marks in case of free ranging wild life areas. Hence, it becomes a need to simulate the
existence of pug marks in an area, so that you can understand well about the pug marks of
different species of wild animals.
Signature
LOADING OF DART
MATERIALS REQUIRED
Darts or special syringes for the darting equipments
Dart needles
Stabilizers if required
Deventing pin
Disposable syringes (2 ml, 5 ml, 10 ml, 20 ml)
Concerned medicaments related to the chemical immobilization
PROCEDURE
Depending on the type of wild animal species select the dart or the specialized syringe with a
fixed volume like 2.5 ml, 3 ml or 5 ml etc.
By using the deventing pin, just remove the pressure of air from the given dart.
Push the plunger in the drug chamber down to the possible extent, so as to hold the required
volume of the medicament to be used for the chemical immobilization.
Now, you have to take the disposable syringe of required volume depending on the volume of
the drug to be taken.
Attach the 21 gauze or suitably sized needle to the hub of the syringe and draw the required
drug to be used to make immobilizing effects in the concerned wild animal under examination.
Draw the immobilizing agent carefully after wearing gloves and/or other protective like
coverall or face cover etc.
Now place the drug into the drug chamber of the dart.
Take the dart-needle subsequent to the selection of the dart needle (whether it is to be a
collared one or barbed one or a plain one).
Attach it now with the drug-chamber end of the dart.
Take the sleeve that is more suitable for that concerned dart-needle and apply over this needle
and close the outlet of the dart-needle well.
Check for the patency of the sleeve and it has to be tight during both placement and
experimental movement over the dart-needle.
Now by either using the ten ml syringe or a mini gas filler, pressurize the dart carefully by
attaching with the air-chamber end or with the fixed stabilizer-end of the dart.
You may have resistance subsequent to the filling of right amount of air into the air chamber of
the dart. At this time, stop pressurizing the dart.
Further, you have to monitor side by side the movement of top layer of drug to the maximum,
in the dart syringe.
Attach the stabilizer to the air-chamber end of the dart syringe, properly.
Keep the duly pressurized dart away from the face or eyes.
Now the dart is ready for the projection.
Signature
Practical Manual VMD-512 25
Practical No.: 7 Date: ______________
OBJECTIVES
1. To demonstrate the capture by usage of bait and trap cage.
2. To have a better restraint by use of squeeze cage.
MATERIALS REQUIRED
Suitable bait materials
Trap cage-model
Squeeze cage-model
PRINCIPLE INVOLVED
Baiting of wild animals has to be understood as a student who wants to learn the application of
trap cage or squeeze cage. Different wild animals may get attracted to different kinds of bait materials
that are available on commercial basis in various countries. However, there are some facts with regard
to the usage of baits in case of wild animals that need to be clarified before proceeding for the actual
trap or squeeze cages in case of wild animals.
BAIT
These are the substances that may lead to the visit of wild animals to the concerned and pre-
planned site for getting them trapped in a scientific manner. Bait materials have to be clarified earlier
about their suitability for the concerned wild animal species. Multiple types of bait materials are
available in reality for use in wild animals. Mobbing reactions may be seen by placing a stuffed owl
near the trap-site set up. Different types of baits are available, in general.
Draw the line diagram of the exhibited design-model and indicate the parts.
Take the trap cage to the concerned area or in the pathway of the concerned animal species that
has more chances of visit by the concerned animal species.
Place the trap cage at suitable position for the entry of the concerned or targeted animal
species.
Select the suitable bait material first.
Hook the bait material inside the trap cage design model, developed.
Fresh feed based bait may be chosen for this purpose.
Check up the hooked bait material, so that even a slightest movement of the cage may lead to
the closure of the trap cage design-model.
The trap should be inspected every 12 or 24 hours.
Signature
OBJECTIVES
1. To dart the wild animal under target correctly by knowing the procedures.
2. To learn about the sites on which dart is to be projected during the usage of different
immobilization equipments.
3. To learn about the precautions to be taken while using the immobilization-equipments.
APPARATUS REQUIRED
Blow gun or blow pipe (1-3 meter length)
Remote distance delivery equipment like short range projector (Pistol) and long distance
projectors (Rifle)
Foot pump to pressurize the individual dart
Pressure-gauze with tube that can be attached with either foot pump or pistol
Prepared dart
Wild animal model for darting
Merits
Silent projection is possible by usage of blow gun.
Highly adaptable for use in small wild animals in particular.
Easy maintenance is possible with this type of immobilizing agent.
Impact developed by usage of this equipment on the wild animal is less when compared to
usage of pistol or rifle.
Less trauma occurs due to usage of this equipment on wild animals.
Demerits
This may not be a fit one for use in distantly existing wild animal (more than 15 meters, in
general).
Chances of missing of the targeted site of targeted wild animal/s or wild animals‟ for darting
are more when the operating veterinarian is not well versed with the usage of blow pipe.
The operator has to have a good stamina with good pulmonary capacity to deliver an effective
blow esp. when the wild animal is in considerable distance.
Merits
Assured strike is there
Fast delivery of drug
No need of blowing air
Demerits
Larger impact when compared to the blow gun
If pressure is not enough as shown in the pressure gauze, then the dart may not be projected
well.
Merits
Can be able to have an assured strike on very distantly standing wild animal.
Safety to the operator is assured from the concerned wild animal.
Signature
GENERAL PROCEDURE
First, there is need to understand that suitable restraining procedure has to be adapted as a
safety measure in all types of wild animals.
Before collection of blood sample, remove dirt and apply alcohol-swab over the selected area.
After collection of blood sample, transfer it carefully into the tube with EDTA.
Also transfer the blood sample into a sterile glass tube without any additive for the separation
of serum.
During withdrawal from the vein in general, always apply pressure over the needle, so that the
pain factor will get minimized in the concerned wild animal species.
Always make it a point to use only the disposable syringes and disposable needles to prevent
the chances of contamination of the site by microbial organisms.
Elephants
Wild pigs
Cranial vena cava is the preferred site for the collection of blood sample in large volume. After
the restraint by physical means, head is extended and lifted up to expose the site of blood
collection. Place the thumb in the right jugular groove and slip along caudally to the
termination of the groove. Right side is preferred since the accidental trauma to the right vagus
nerve is less likely to cause the pro0found cardiac as well as the respiratory dysfunction. Direct
the needle slightly caudally and dorsally toward the left dorsal scapula for effecting the blood
collection.
Caudal auricular vein may be used after enhancing blood vessel by application of tourniquet
around the base of the pinna.
Non-Human Primates
Saphenous vein may be used after applying pressure at the proximal site to the collection area.
Femoral vein (medial aspect of thigh) may be used.
If it is a large sized lizards like monitor lizard, jugular vein may be of useful for the collection of blood
sample. Tail vein may also be used in case of lizards.
Signature
OBJECTIVES
1. To learn about the details related to the restraint of elephants (physical and chemical) in a safe
manner models and slides.
2. To learn about the features pertaining to the restraint of non-human primate-Bonnet macaque.
MATERIALS REQUIRED
Immobilization equipments like blow pipe, pistol and rifle
Immobilization related drugs that are commonly used like xylazine and/or etorphine with
acepromazine, atropine sulphate, doxapram hydrochloride, suitable antidotes like yohimbine
and diprenorphine hydrochloride
Infrastructures related to the restraint of elephant like elephant-chain, ropes, trained elephants
etc.
Elephant Hook
These are generally applied on elephants and it helps to ensure the standing-still in one place
without moving away to another area in general.
However, the legal status for using the elephant hooks has to be looked into.
Chaining of Elephant
Animal ethics related issues are to be taken care of while using such physical restraint
measures in case of elephants. However, it appears better to pad the chains that are used for
physical restraint of the elephants.
This helps a lot in minimsing the probabilities of injuries on the limbs of elephants to a greater
extent.
It is to be understood that skin related wounds may take comparatively a long time for a better
healing because of poor circulation of blood in skin of elephants.
Krall
Newly caught elephant–calf may be kept in the krall that is erected with thick bamboo sticks
etc.
This is one of the better means of exercising the physical control over the movement of the
newly caught wild elephant.
Usage of Xylazine
Xylazine is the sedative, analgesic and muscle –relaxing drug. Darting may be done in the
thigh regions or in posterior regions correctly. The dose rate is 0.08 mg per Kg body weight to
be given I/M route by blow pipe or pistol or rifle or by syringe. In case of excited elephants, a
higher dose of 0.15 mg per Kg body weight may be given. Atropine sulphate may be given at
the rate of 4 to 5 mg/100 Kg body weight to avoid the hypostatic congestion and adverse
cardio-depressant effects.
However, the clinical condition during the time of immobilization is most important to be
assessed by the attending veterinarian. Induction may not be smooth if xylazine is given by I/V
route and in general xylazine is not given by I/V route. However, it is to be understood that
xylazine may be given intravenously at one half the intramuscular dose (0.04 mg per Kg body
weight). Yohimbine is the antidote and twice the dose of xylazine may be the dose of
yohimbine by I/V route.
Precautions
a. The elephant should not be in sternal-recumbency for more than 10 minutes on safe side. This
is due to the lack of pleural space that can lead to cardiac as well as respiratory system-
embarrassment due to the heavy weight of visceral organs pushing the thorax.
b. The rope in case of captive elephants should be checked earlier for the presence of adequate
length. If not, rope or chain needs to be changed because the inadequate length of rope or chain
may lead to medical problem like choking if the effects of immobilization start.
c. Finish the clinical procedures as soon as possible.
d. Take care of hypothermia or hyperthermia that can develop during the immobilization event in
an elephant
e. Doxapram hydrochloride may be given at dose rate of 2 mg per Kg body weight by IM route,
in general to stimulate respiratory activities at the end of the clinical procedures.
Usage of Etorphine
Dose is 1 mg per 450 Kg body weight, in general as the case with African elephants. This is a
narcotic drug and hence, human health hazards are common with this if it spills on body
regions. It is better to overdose with etorphine than to under dose. Hence, one has to be more
careful while handling this drug in case of wild animals of any species.
Precautions
Take care of the immobilized macaque because other macaques nearby in the troop may
disturb that or try to bite that animal.
Try to avoid the darting while the animal is near the water-source.
Avoid the darting of non-human primate while in height because it may lead to the fracture of
bones during falling from a great height. If it is to be done, take effective precautionary
measures to avoid the impact of falling on the ground.
Plan about what to do after the capture-operation.
Signature
Special features
Largest of all living land animals
Skin is thinner in shoulder and forelimbs but thickest over the hindlimbs and hindquarters.
Sweat glands are present in sparse manner but above toe nails, sweating may be seen.
Hairs are more prominent in young animals than the adults.
Presence of air-filled sinus activities are present in the skull
Dental formula:
o Incisor : 1-1/0-0
o Canines : 0-0/0-0
o Premolars : 3-3/3-3
o Molars : 3-3/3-3
Generally two pairs of teeth are present at a time and the anterior tooth is pushed forward by the
backwardly placed tooth. Generally, six complete sets of grinding teeth are present called simply as
molars. Complex ridges or lamellae are present on each tooth.
Fibro-elastic fatty pad is present in all the four feet.
Trunk is a specialized structure that is heavily muscled one and is devoid of bones. Nearly
seventy per cent of air is taken through the trunk.
Males have tusks in pair which are the modified dentine tissues.
Tushes (smaller ones) may be present in some females.
Secretion from temporal glands is a unique feature esp. in case of males of Asiatic elephants
Mammary glands are present in between the forelegs like non-human primates.
Stomach is an unusual cylindrical shape.
Total length of small intestine and large intestine together is 35 meter (hence, it takes 24 hours
for the digestion in case of elephants)
Vertebral formula is C7 T20-21 L3-4S4-6 Cy18-33
Absence of gall bladder
Lack of pleural space
Anterior venacavae are double and bifid apex of heart is seen unlike in most of the mammals in
which a single pointed apex is generally seen.
Anal fold is the characteristic loose skin present covering the anal regions.
Vertically placed limbs are present and are sturdy in nature to support a huge body weight.
Penis can reach the length of about 40 cm. When erected, it assumes a „S‟ shaped flexure.
Clitoris can reach the length of about 40 cms
Vagina is separated from vulva by an uro-genital canal that averages between 40 and 100 cm
long.
Penis is similar to horse that is capable of making search like motion.
Testis may never descend and are placed in abdomen through out the life
Lobulated kidney
Signs of musth
Restlessness
Aggressive behavior
Practical Manual VMD-512 38
Dribbling of urine
Secretion of black colored oily fluid from cheeks
Disobedience of the commands given in case of captive male elephants
The normal body weight of the adult and young Asiatic elephant
Where,
L= Length from base of forehead (mid-point of supra-occipital crest) to the base of the tail in
centimeters
G= Chest girth in centimeters
HAEMOTOLOGICAL PARAMETERS
BIOCHEMICAL PARAMETERS
BUN 75-200 mg %
Creatinine 1.3-1.8 mg %
Total protein 8.0-9.2 gm %
Albumin 2.3-3.5 gm %
Cholesterol 41-61mg %
Total bilirubin 0.1-0.3 mg %
Direct bilirubin 0.0-0.1 mg %
Glucose 75-200 mg %
INTRAMUSCULAR INJECTIONS
Use one and half inch needle in the large triceps area.
Long needles may be necessary for the I/M injections into the hind quarters of elephants. Dose
up to 30-50 ml may be given in one site.
However, it may produce swelling. Hence, monitor the elephant frequently. Darts may be used
for I/M injections.
INTRAVENOUS INJECTIONS
Use the blood vessels at the posterior aspects of ears that are easily accessible for I/V
injections.
Large veins on the proximal foreleg and disto-medial rear leg can also be used for this purpose
but be careful with the safety measures.
In young elephants, the large superficial vein running up the medial aspect of the hind leg is a
good site for I/V injection.
SUBCUTANEOUS INJECTIONS
Not much desirable as the I/M route.
Use it only when specifically recommended.
Shoulder-skin area or anal fold area may be used.
ENEMA ADMINISTRATION
Use a well lubricated sleeved arm and insert into the rectum
Insert as far as possible
Take a lubricated hose
Pass the hose into the rectum aiming the hose antero - dorsally.
Pass the hose till you encounter the resistance
Now administer the enema solution (liquid paraffin or soap-water may be used as enema).
Racking may be done with manual removal of dung material in case of constipation based
colic cases.
Useful in case of severe constipation
Signature
OBJECTIVES
To learn the technique on the utilization of pulse oximeter for capture related and clinical
investigation purposes in animal
APPARATUS REQUIRED
Pulse oximeter
Sensors
PRINCIPLE
The color of blood is the function of oxygen saturation, the color change with oxygen
saturation is due to the optical properties of the hemoglobin molecule and more, specifically
the heme component, as the blood deoxygenates, it becomes increasingly less permeable to red
light. The tissue then losses its pink appearance and takes a blue tint, an oximeter only needs to
measure the blueness of the arterial blood, venous blood and any other major absorbents of
light between the two halves of the sensor and to display value in terms of saturation
The presence of light emitting diodes (LED) of given wavelength at one end and a photo-
detector at the other end helps to measure the wavelength of light during exposure. It is the
sensor that converts the light intensity into electrical current.
The difference in the intensity of the transmitted light between the LED is caused by the
difference in the oxygen contained within vascular bundle and the relative amount of light that
is transmitted to the photo-detector forms the basis for the pulse oximeter, to compose the
arterial hemoglobin saturation.
PROCEDURE
In animals, first make an effective restraint with suitable technique
The sites chosen for application of electrodes:
o Ear, tongue, rectal mucosa, lips, toes, prepuce, etc
o Generally tail region should be chosen
Since the skin folds may obstruct the assessment, before applying the procedure, the hair
material should be clipped away, before applying the pressure and this also facilitates the
avoidance of slipping of the probes /electrodes.
In aviary species: Site for attachment of light emitting diode (LED) is the point just below the
hock joint
MERITS
Useful for continuous assessment of saturated pressure level of oxygen (SpO2)
Easy to operate
It is not on invasive procedure
More useful for the assessment of arterial blood flow
It is an early warning system, in critically ill wild animals that are in recumbent and comatose
condition
Rapid technique
Practical Manual VMD-512 43
DEMERITS
Lack of more scientific information on wild animals
If low perfusion occurs, a negligible volume of blood flows through the artery
Signature
CAPILLARIASIS IN COCKATOO
The Eggs are more or less barrel shaped with fine bipolar plugs resembling Trichuris eggs.
These worms are commonly called as 'Thread worms' and are frequently found in newly
imported macaws and in aviaries.
Further they have been encountered in wild passerines. Depending on the species the life cycle
is direct or indirect involving earthworm as intermediate host.
STRONGYLOSIS IN SQUIRRELS
This round worm is one of the commonly observed nematodes in squirrels in addition to
Ascarids. These worms are usually present in large numbers.
The eggs are oval shaped, thin shelled with compact yoke mass. The life cycle is direct through
third stage infective larvae present in the vegetation
BUNOSTOMIASIS IN BLACKBUCKS
Bunostomum spp. is considered to be one of the most dangerous nematodes in animals. These
hook worms have a well developed buccal capsule with well developed chitinous plates and
lancets.
The infection occurs through penetration of infective larvae into month or skin. The eggs are
like strongyle with thin shell and are darkly pigmented with compact yolk mass and parallel
sides.
Signature
CROCODILES
BASKING
This is the procedure by which the heat is obtained by the crocodiles and in this the mouth
parts are kept opened, so as to have more surface areas of oral region exposed to the direct sun
rays.
Since the crocodiles are the ectotherms, the animals need to depend on the external heat
resources.
PHYSICAL RESTRAINT
Take utmost care in the physical restraint of crocodiles.
Use a longer pole with a snare at the end.
Carefully catch the crocodile with the snare and bring it to the ground by dragging in water
Better to have this near a tree or wooden plank that is strongly grounded vertically.
Close the eyes of the crocodile with a gunny bag or thick cloth
Experienced persons will immediately hold on the head and tie the mouth with a rubber tube.
At the same time, the tail has to be secured and then the legs have to be secured (forelimbs and
hindlimbs separately)
Now, you can undertake the clinical inspection.
CHEMICAL RESTRAINT
In reptiles, ketamine may be given at dose rate of 22-44 mg per Kg body weight by I/M route
Larger reptiles need a smaller per kilogram dosage than the smaller ones.
Surgical anesthesia may be given with dose rate of 55-88 mg per Kg body weight.
Recovery may be a prolonged one when ketamine is given esp. in sick or debilitated reptiles.
Xylazine may also be used for the chemical restraint of crocodiles.
Neuromuscular blocking agent like succinylcholine may be given at dose rate of 3-5 mg per kg
body weight by IM route for muscle relaxation effects. Etorphine is most successfully used in
crocodilians like chelonians.
Practical Manual VMD-512 55
Halothane may be used in crocodiles like in any other reptiles.
CLINICAL EXAMINATION
Observe the crocodile before restraint
Watch the movements of the crocodile
See for any abnormalities on the body like hunch-backed appearance, bleeding wound or
injuries, swelling on skin region, dragging of limbs, eye-status in general etc.
Collect the fecal samples for parasitic examination.
BLOOD COLLECTION
First they need to be restrained well and it needs experienced animal keepers in a zoological set
up. More care is to be taken before the sampling technique in case of crocodiles. This will
surely help to avoid the casualties associated with the sampling technique in case of crocodiles.
Toe-nail clipping may be done to obtain minimum amount of blood or to obtain the blood
smears from the oozing blood sample. However, for getting of large volume of blood sample,
one has to go for other techniques associating the supra-occipital site or cardiac site, in these
species.
CARDIOCENTESIS TECHNIQUE
Identify the line drawn in between the lower line linking both lower margins of fore limbs.
Count the horizontal rows of regularly placed scutes from this line in the ventrally placed and
well restrained crocodile with its mouth tied by a rubber tube.
th th
The 7 to 9 complete horizontal row of scutes may be identified in the crocodile.
Centre point may be chosen for piercing by a disposable needle of 20 gauze with 1.5 inch
length.
Then attach the 10 ml syringe with the syringe after sighting the gushing of blood sample
through the needle and carefully withdraw the blood sample.
COMMON PARASITES
Collect the fecal sample in fresh condition. Examine for evidences of the parasites using
standard laboratory techniques :
Commonly occurring parasites are :
o Leeches are commonly seen on the crocodiles.
o Coccidian parasites like Eimeria sp., trichomonas sp., ascarids etc. are encountered in
crocodiles
BREEDING FEATURES
In crocodilians, scent glands in the lower jaw and cloaca secrete attractant chemical agents.
Neck–rubbing and tail-thrashing are the courting activities in addition to the circling around
one another.
In females, mild turning of the body with raising of tail is seen in females for mating purpose.
These animals are the egg layers.
About 50-70 eggs are laid in a clutch.
Fifty to seventy days is the incubation period.
Before egg laying, the female crocodile builds the nest of loose vegetation and twigs.
SEXING
This is done by deep digital palpation of the penis in the cloacal regions in case of males.
Absence of feeling of penis is the positive clue for the female crocodiles. Appearance wise
differentiation is difficult in general. However, it requires more experience.
Signature
SERPENTINES
BASELINE FEATURES
Ectotherm or cold blooded animal
Dorsum is full of scales
Head is flattened
Transparent fused eyelids are present
External ear is absent
There is no tympanic membrane or middle ear cavity
Tongue is forked and is lengthy
Fang is a specialized structure and when unused, it is covered by a membranous flap. Venom
glands secrete venom
Three chambered heart is present due to the incomplete ventricular septum.
Diaphragm is absent
In most snakes, the left lungs is reduced
Urinary bladder is absent like crocodiles
Cecum is present in some snakes of boidae and in python
Oviparous or ovoviviparous
Heat pits are present in vipers, boa etc.
Spurs are present in boids and are the vestigial limbs
Elongated spindle shaped liver is present
Renal portal system is an important component of venous system in any reptile
ECDYSIS
It means the shedding of skin periodically. The skin is mostly shed in one piece except the
giant snake and the shedding of skin begins from the head region.
Incomplete shedding occurs due to general illnesses including the mite infestation, dermatitis,
trauma and also due to incorrect humidity or handling, malnutrition etc. During the shedding,
the skin has a glossy blue coloration due to the presence of lymph in between two layers.
The shedding period is preceded by an inactive period for a week, during which time the eyes
may appear dull or bluish white.
PHYSICAL RESTRAINT
Use transparent tube for a better control
Snake hooks may be used
Tongs may also be used for a better control of snake
Never restrain the python by a single person. Always a minimum of three persons is a better
one for effective restraint of the pythons.
Regardless of the species, the head needs to be secured well in case of snakes to avoid being
bitten
Safety to both snake and the operator is the important feature to be taken care of during the
physical restraint of the snakes of any species
CHEMICAL RESTRAINT
Ketamine may be used in all reptiles including snakes. Dose varies with size and nature of
snake. In general, the dose rate of ketamine is 22-44 mg per Kg body weight by Intra-muscular
route and for surgical anesthesia, it may be at 66-88 mg per Kg body weight.
Halothane may be used in snakes like in all reptiles. Induction is generally rapid at
concentration of 3-4 % given by a face maksk or an induction chamber. The gas mixture may
have 1:3 combination of oxygen and nitrous oxide respectively and anesthesia can be
maintained at 1-1.5 % halothane and recovery is usually rapid.
Practical Manual VMD-512 58
BLOOD COLLECTION FROM TAIL-VEIN
Place the snake on its back.
Position the needle about one third the distance back from the vent toward the tail. After the
advancement of the needle through the skin gentle aspiration is applied until either bone or
blood is encountered.
However, it is to be remembered that the size of the disposable needle to be used in case of
snakes varies depending on the size of snake.
COMMON PARASITES
Entamoeba invadens which is a commonly encountered protozoal organisms in snakes. Banana
shaped intraerythrocytic hemogregarines in blood smear of water snakes, Eimeria in fecal
samples of snakes, haemoproteus sp., renifer eggs etc. are commonly seen in snakes.
The commonly encountered snake mite is Ophionyssus sp.
Similarly, among ticks, it is the Aponomma species which is frequently encountered in
serpentines.
BREEDING FEATURES
Most of the snakes are viviparous in nature. Some are ovo-viviparous in nature. (embryo inside
the egg that develops inside the body of the mother and young ones are born. Eg. Sand boa
SEXING
Oophiprobe is used for sexing. The snake is kept on its back and carefully restrain the snake
without getting bitten. Insert the oophiprobe and if it passes to more distance (at least 9-15 sub
caudals), it is a male and if it passes only to a shorter distance (not more than 1-3 sub-caudals),
it is female.
Male snakes tend to have a longer tail than the female snake.
Signature
OBJECTIVES
To know the management aspects of neonatal elephant calf that is orphaned. (For this, you are
expected to know the situations in which the need arises, any facts lying related with feeding or
care taking of such elephant calves, any veterinary care to be taken specially in such occasions
etc.)
REASONS
Many times, the calf of the elephant gets orphaned due to many reasons like death of the
mother or care taking elephant.
Calf may get trapped in the mud or other problematical areas like pit etc. and finally the cow
elephant may leave the calf.
Rejection of the calf by mother if it has painful mammary glands or mammitis.
CLINICAL EXAMINATION
Examine the body parts of elephant calf including base of tail, perineum, external and internal
ear regions, foot regions, trunk and oral commissures for evidence of any injuries
See for the general signs of health in an elephant calf
Check the body temperature of the animal
Check for any pain causing lesion/areas to rule out the myositis like problems
Examine for evidence of any parasitic infestation
Examine for signs of constipation like straining of animal during defecation with solid dung or
signs of abdominal contraction but with no defecation activity
Examine for signs of diarrhea
Examination of samples
Peripheral blood smear To rule out the differential leukocyte count and evidence of
haemoprotozoa
Whole blood in tubes For biochemical investigations like total protein, albumin, globulin, A/G
that can be centrifuged ratio, total bilirubin, direct bilirubin, indirect bilirubin, BUN, creatinine,
for serum separation aspartate aminotransferase, alanine aminotransferase, total cholersterol,
calcium, phosphorous, alkaline phosphatase etc.
Time of feeding
Increase the frequencies of feeding in case of calf unlike the adult elephant and in the
beginning feeding is to be done in day and night.
When the calf reaches 2 or 3 months of age, night feedings may be gradually stopped.
When the calf is 9 months old, 4 feedings are enough per day.
Amount of feeding
The elephant calf consumes 1.5 to 2 litres of diluted milk at a time in one to two hours and 12
to 16 liters may be needed in a day, in general.
Try to remember that always leave them little hungry.
Over feeding is always more harmful than slight under feeding of calf
TYPES OF FEED
Protective immuno-globulins may be given soon after the birth and this is in-order to establish
the calf‟s immune status.
Older calf should be given immuno-globulins by subcutaneous administration because these
would not be well absorbed if given by oral route.
The volume of the colostrums consumed by a newborn elephant during the first 24 to 48 hours
of life is approximately 2 to 10 litres.
Tender coconut water may be given frequently to quench the thirst.
Coconut oil is the nearest artificial replacer to the fat of the elephant‟s milk.
Milk Protein (%) Fat (%) Carbohydrate (%) Solids (%) Fiber (%)
Cow‟s milk 2.8-3.6 3.1-5.2 4.0-5.5 8.5-19 -
Human‟s milk 0.7-2.0 1.3-8.3 5.0-9.2 8.5-15 -
Elephant‟s milk 19.9 0.63-6.2 4.0-8.36 8.27-17.3 -
Elephant calf under artificial feed formula should always gain a minimum of 0.5 kg body
weight per day (recordings are available even with a weight gain of about 1.5 kg per day for
extended periods of time).
Weight Energy requirement per day Rice based formula required per day
100 kg 6000-8000 kcal 400-550 oz
200 kg 16,000-20,000 kcal 1100-1333 oz
Signature
Note
If the bird is with crop, pass the tube directly into the crop
If the bird is without crop, pass the tube up to the distal end of esophagus; if not, this may lead
to the development of iatrogenic trauma to the proventriculus and/or gizzard.
SAMPLING IN BIRDS
Sampling in case of birds need special care and management measures. In general, the bird
should not be held tightly.
Always use gentle methods during the holding procedures in case of any aviary species.
Maintain calmness at the place of sampling to avoid the excitement of the bird under
investigation
Cover the eyes of the bird regardless of the species to minimize the stress factors that may act
on the concerned aviary species.
Plan about the kind of samples required well in advances.
ESTIMATION OF WEIGHT
Body weight should be approximately known for each species.
Preferably an electronic balance may be used to assess the body weight of the concerned aviary
species.
ORAL ROUTE
Administration of medicines by oral route
The medication may be given by food or water.
Merit
Easy to administer
Animal keeper or the owner himself can administer
Demerit
There is no assurance that the bird will take adequate amount of drug mixed water or
food esp. while in sick status.
Some birds rarely drink water in general.
Tastes of the drug has influence on the intake of water or water containing the drug.
INTRAVENOUS ROUTE
Administration of medicines by I/V route
This route is mainly used in case of emergency or in case of larger sized birds like cranes,
storks etc.
Take care of the hematoma development or bleeding that may often occur.
This is very important esp. in case of smaller sized aviary species.
The veins to be used for the administration of medicaments are dealt under the chapter of
„Collection of blood sample‟.
INTRA-OSSEOUS ROUTE
Administration of medicines by intra-osseous route
This is useful when I/V route or S/C route is not possible to be used due to smaller size of the
birds etc.
Intra-osseous cannula is used to administer blood, fluids, colloids etc.
Site
Proximal tibia for short-term therapy
Distal ulna in medium sized to large birds that requires days of therapy.
Merits
Reduced patient-resistance is observed during the adaptation of this route of administration of
drugs.
Less stress is experienced when compared to the repeated venipunctures.
In birds in which the venin can not be traced as in case of highly sick birds or in smaller sized
birds, this route may be of tremendously useful for drug administration.
Signature