M I C R O B I A L M E T A B O L ISM

E N Z Y M ES

C H APT ER 5
M etabolism: sum of all chemical reactions
within an organism.

M etabolism:

1. A nabolism:

2. C atabolism:
 M etabolic Reactions

‡ A metabolic pathway is a sequence of

enzymatically catalyzed chemical reactions
in a cell which are determined by enzymes.

1. E xergonic

2. E ndergonic
E nzymes:

Biological catalysts

Protein in nature

Reaction runs 8-10 billion times

faster
Energy Requirements of a Chemical Reaction

Figure 5.2
 E nzyme Structure

Holoenzyme

1. A poenzyme

2. Cofactor
1) Coenzymes: N A D + , FA D, CoA
2) M etal ions
Components of a Holoenzyme

Figure 5.3
M echanism of E nzymatic A ction

Active site of an enzyme

E nzyme-substrate complex

Specificity of active site

The Mechanism of Enzymatic
Action

Figure 5.4a
 Relationship between
E nzyme Structure and F unction

10, 20, 30 and 40 structure of an enzyme

C hange of enzyme shape

C hange of active site----denaturation

F actors affecting enzymatic activity

Temperature

pH

Substrate concentration

Inhibitors
Effect of Temperature on Enzyme Activity

Figure 5.5a
Inhibitors

Competitive inhibition

Noncompetitive inhibition
Enzyme Inhibitors: Competitive Inhibition

Figure 5.7a±b
Enzyme Inhibitors: Competitive Inhibition
Enzyme Inhibitors: Noncompetitive Inhibition

Figure 5.7a, c