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A Global Initiative
R&D Unit
Production
Isolation: Screening of ten thousand native microbial strains from soil and water of various
sources
After processing of multiple samples of soil and water from various sources, thousands
of bacteria, fungi and actinomycetes wese isolated and enumerated, however, only
industrially potential strains were maintained and preserved for product formulation
Strain Sequence in NCBI GenBank
Bacteria / Fungus Property Accession No. based on
16S rDNA/ 18S rDNA
Sequence
Bacillus subtilis Protease producer DQ267974.1
Fungal sp. BDP 77 Cellulase producer EU367012.1
Bacillus amyloliquefaciens strain CPB-5 Alkaline protease producer GU269624.1
Bacillus pumilus strain CPB-6 Amylase producer GU269625.1
Bacillus licheniformis strain CPB-8 Amylase producer GU269626.1
Bacillus pumilus strain CPB-9 Xylanase producer GU269627.1
Bacillus subtilis strain CPB-10 Orgnic acid producer GU269628.1
Bacillus licheniformis strain CPB-14 Orgnic acid producer GU269629.1
Candida sp. BIBHUCPB 16 Phytase producers JF909923.1
Trichoderma sp. BIBHUCPB 15 Cellulase producer JF909922.1
Bacillus pumilus strain CPB13 Orgnic acid producer GU322346.1
Bacillus pumilus strain CPB11 Protease producer GU322345.1
Firmicutes bacterium CPB4 Orgnic acid producer GU322344.1
Bacillus subtilis strain CPB-3 Orgnic acid producer GU269631.1
Bacillus licheniformis strain CPB-2 Protease producer GU269630.1
Azotobacter ATC-CPB-017
Strain Improvement
Mutagenesis for increased expression of enzyme
UV mutagenesis
Chemical mutagenesis
Bio-fertilizers
Liquid product formulation
Liquid filling process
Powder formulation process
Carrier selection
Shelf life
Village 4
Village 5
Village 3
Village 1 2
Village 2 Soil Samples
1 from District
Soil samples
from village 3
Soil Processing
4
Microbial Strains
5
Best selected strains
a b c d e f g
B. subtilis P. fluorescens Rhizobium Azotobacter Azospirillum ‘P’ Solubilizer Trichoderma
(BS) (PF) (RZ) (AT) (AZ) (PSB) (TR)
Tolerance to pH
& Temperature
8
If sensitive, Induction to pH &
temperature stress
9
Strain transferred to
Production unit
Farmer – Back to
Farmer Model
Care Pro Bioscience:
Manufacturing Facility
Manufacturing Facility
Location: I-59, UPSIDC, Site-V, Kasna, Gautambudh Nagar, Greater Noida, UP
Ownership: Own
Site acquired: 2009
Construction: 2010 to 2014
Semi-Operational: 2014-15
Plot Area: 3600 sq. meter
Construction: 70000 sq. ft. of RCC
Status: Building Completed and functional
Finance:
No bank loan, invested from own revenue
Technology Validation Registration Certificates
INDIA USA and Turkey
SCS CERTIFICATE
Intellectual Property Right
Patents
Crops: Tomato, Brinjal, Banana, Capsicum, Chilli, Onion, Lady-finger, Papaya, Wheat , Paddy, Citrus, Tea, Coffee & Black pepper
Botanical Pesticides
A 100% organic and eco-friendly botanical Insecticide composed of plant and seed
extracts.
Crops: Wheat, Barley, Oats, Maize, Pulses, Cotton, Jute, Sugarcane, Groundnut, Vegetables,
Plantation crops
Crops: Mango, Pomegranate, Date palm, Coconut, Apple, Custard apple, Guava, Cucumber, Onion, Garlic, Millets, Wheat, Paddy,
Tomato, Chilly, Sugarcane, Turmeric, Banana & Cotton
A liquid formulation, botanical blend of several herbal essential oils and plant extracts
containing various natural macro and micro elements, amino acids, vitamins and
phyto hormones like cytokines, auxins, etc
Crops: Mango, Pomegranate, Date palm, Coconut, Apple, Custard apple, Guava, Cucumber, Onion, Garlic, Millets, Wheat, Paddy,
Tomato, Chilly, Sugarcane, Turmeric, Banana and Cotton
A liquid formulation, blend of herbal essential oils derived from plant extract which improves
the soil quality, health & structure resulting in enhancing the root growth and crop yield
Application Methodology (Per Acre)-
Soil Application: Mix 125ml product in water thoroughly and immediately apply the mixture
on the crops
Foliar Application: Mix 125 ml of product in 100L of water thoroughly and immediately spray
to the root zone of the plant
Drip Irrigation: Mix 125ml of product in 200L of water thoroughly and immediately apply to the
plant through irrigation system.
Dosage- 125ml per acre
Crops: Mango, Pomegranate, Date Palm, Coconut, Apple, Custard Apple, Guava, Cucumber, Onion, Garlic, Millets, Wheat, Paddy, Tomato,
Chilly, Sugarcane, Turmeric, Banana, Cotton, Castor
An Innovative plant origin product , rich in Nitrogen, contains all the essential
nutrients and amino acids for excellent plant growth, vigor and higher yield and
T suitable under adverse condition, even to the most deficient crops grown in the
O poorest soil
N
I Application Methodology (Per Acre)-
K Mix 1 kg product in 200 L of water and spray on the crops
Make 2 to 4 foliar spray per season at an interval of 10 to 12 days of first application.
A
First application should be made after 20-25 days of sowing/transplanting.
A
Do foliar spray in perennials and plantations crops after harvest of previous crop till
Plant pre-flowering stage
Nutrient Dosage- 1kg per acre
Concentrate
Crops: Paddy, Tomato, Groundnut, Cotton, Brinjal, Potato, Sugarcane, Pulses, Fruits, Wheat,
LadyFinger, Potato, Sorghum, Soyabean, Ornamental Plants, Greenhouse & Nursery Plants
Bio-Fertilizer & Plant Growth Promoter
Crops: Fiber crops, Plantation crops, Vegetables, Cereals, Sugar crops and Fruits
A liquid biofertilizer
containing
Application Methodology (Per Acre)- Rhizobium Bacteria
Seed Treatment: Mix 50 ml product in water to
make slurry. Mix the entire seed in prepared slurry
for coating the seeds and dry under shade before
sowing
Seedlings Treatment: Mix 500 ml product in 100 L
of water and dip the seedling roots for 20 – 30
minutes before transplanting
Soil application: Add 500 ml product with 120 g of
soya flour extract in 50 L water and activate for 24 h
and mix with 250 Kg of FYM/vermicompost/organic
compost. Keep the mixture for 7-10 days
Application Methodology (Per Acre)-
Application Methodology (Per Acre)-
Seed Treatment – Mix 50 ml product in
Soil Application– Mix 500 ml water to make slurry. Mix the entire seed
product in 250 kg well decomposed in prepared slurry for coating the seeds
FYM/ compost. Blend the mixture and dry under shade before sowing
and broadcast it over 1 acre land in Seedlings Treatment – Mix 500 ml
the standing crop and irrigate the product in 100 L of water and dip the
field seedling roots for 20 – 30 minutes
before transplantation
In case of Horticulture crops it should Soil Application-
be applied in the effective root zone i. 500 ml product with 120 g of soya
Drip Irrigation – Mix 500 ml of flour extract in 50 L water to activate
Product in 200 L of water and apply in (with in 24 h)
the field ii. Mix activated suspension with 250
Foliar Application- Mix 500 ml of Kg of FYM/vermicompost/organic
Potash care in 100 L of water and compost. Keep the mixture for 7-10
days and broadcast
spray near the root zone
Dosage- 500ml per acre
Dosage- 500ml per acre
Crops: Cereals (Wheat, Paddy, Maize, Barley), Pulses (Soybean,
Green gram, Chickpea, Black gram, Pigeon pea), Oil seeds
Crops: Cereals, Paddy, Wheat, Pulses, Citrus, Pomegranate, (Sunflower, Ground Nut, Mustard.), Fibrous(Cotton, Jute),
Ginger fruits, Spices etc Vegetables, Fruits and Plantation crops.
Application Methodology (Per Acre)-
¶ Soil Application– Mix 500 ml Product in 250 kg well
decomposed FYM/ compost. Blend the mixture and broadcast
it over 1 acre land in standing crop before irrigating the field
In case of Horticulture crops, it should be applied in the
effective root zone
¶ Drip Irrigation – Mix 500 ml of Product in 200 L of water and
apply in the field
¶ Foliar Application- Mix 500 ml of Product in 100 L of water and
spray near the root zone
Dosage- 500ml/acre
Crops: Cereals, Paddy, Wheat, Pulses, Citrus, Pomegranate, Ginger fruits, Spices etc
Disease Protectants
Bio-Fungicides
A carrier based (powder) bio-fungicide containing high cell density of
Trichoderma spp.
Target Crops:
Eggplant, Potato, Chilli, Tomato, Cucumbers, Cut and Pot flowers, Orchards, Vineyards
Ornamentals in Greenhouses, Lawns and Nurseries etc.
Disease Protectants
Bio-Fungicides
Crops:
Wheat, Maize, Potato, Cucumber, Eggplant, Tomato, Cut flowers, Chilly, Orchards, Vineyards, Ornamental Plants
Greenhouses/Nursery Plants and Lawns
Bio-Insecticides
A powder based bio-insecticide containing spores of Bacillus
thuringiencis var. kurstaki, a naturally occurring entomopathogenic
bacterium
Application Methodology (Per Acre)
Foliar Application: 1Kg product in 100 L water
Dosage: 1kg per acre
Crops:Cotton, Pulses, Oilseeds, Vegetables, Tea, Cashewnut, Coffee, Alfaalfa,
Corn Sunflower, Legumes, Root & tuber crops, Fruits
Crops: Cereals, Fruits, Oil seeds, Ornamental crops, Pulses , Vegetables & fiber
crops.
Performance of Products
Radish (Rabi, 2018-19)
Variety: Arka Nishant
Chemical BioMix-I
+Flower-Care
Village: Chitti, Block Dankaur
Farmer’s Name: Ajay
Sowing Date: 22.12.18
Plucking Date: 04.02.19
Organic Agriculture in India
Successful Product trial under Organic Farming
Using Biomix as critical technology intervention
S.No State Total No. of Districts Total No. of Villages Area Covered (Ha)
1 UP 84 2375 49700
6 Sikkim 2 7 4460
7 Rajasthan 1 51 1000
• Covers more than 15 % of the total certified/ under conversion organic area of India
• Highest area coverage by any of the organizations
• Crops include cereals, pulses, oilseeds, fruits, vegetables, spices and medicinal plants
Application of BioMix: India
Sapling for
Plantions
Treated Untreated
Treated Untreated
Treated Untreated
Treated Untreated
Bio-pesticides
Projected Bio-pesticide Global Market** 2020 USD 3379.37 Million
*Source: Global Bio-fertilizers Market by Types (Nitrogen fixing, Phosphate Solubilizing, and Potash Mobilizing), Applications & Geography - Trends &
Forecasts to 2017.
**Bio-pesticides Market by Active Ingredient (Microbials & Biorationals), by Type (Bio-insecticides, Bio-fungicides, Bionematicides & Bioherbicides), by
Application, by Formulation, by Crop Type & by Geography Global Trends & Forecast to 2019
The Proposal
Fermenters: 3000 L x 1;
5000 L x 1; 500 L x 6; 250 L x 1 ; 100L x 5
Sales Revenue - 9.26 234.31 501.42 625.93 717.59 921.38 985.88 1054.89 1128.74 1207.75
PBDT 0 1.36 68.33 159.52 213.12 259.45 336.77 369.73 405.43 444.05 485.64
PAT -2.07 -3.15 42.30 103.95 140.20 172.70 226.41 249.58 273.71 299.83 327.94
Capital Deployed 13.38 22.47 71.20 175.14 315.35 482.14 702.64 946.30 1214.10 1508.02 1835.96
ROI -82.42% 0.00% 101.55% 71.39% 49.06% 37.67% 33.06% 26.71% 22.65% 19.88% 17.86%
Profitability Statement
(Millions USD)
Year
1st 2nd 3rd 4th 5th 6th 7th 8th 9th 10th 11th Total
Capacity Utilized 0% 5% 30% 60% 70% 75% 90% 90% 90% 90% 90%
Production (M. Ton) - 193,750 4,582,500 9,165,000 10,692,500 11,456,250 11,456,250 11,456,250 11,456,250 11,456,250 11,456,250 93,371,250
Sales
Sales Revenue - 9.26 234.31 501.42 625.93 717.59 921.38 985.88 1,054.89 1,128.74 1,207.75 7,387.14
Total Sales Revenue (A) - 9.26 234.31 501.42 625.93 717.59 921.38 985.88 1,054.89 1,128.74 1,207.75 7,387.14
Cost of Production
R & D Exp @ 2% - 0.19 4.69 10.03 12.52 14.35 18.43 19.72 21.10 22.57 24.15 147.74
Raw Material - 5.11 123.77 253.89 303.97 334.41 426.81 448.15 470.56 494.08 518.79 3,379.53
Packaging Cost - 0.03 0.73 1.50 1.81 2.01 2.56 2.69 2.83 2.97 3.11 20.25
Power & Fuel - 0.00 0.08 0.16 0.19 0.21 0.27 0.28 0.30 0.31 0.33 2.14
Wages & Salaries - 0.01 0.31 0.64 0.77 0.85 1.08 1.13 1.19 1.25 1.31 8.55
Administrative Cost - 0.10 2.35 4.82 5.77 6.35 8.10 8.50 8.93 9.37 9.84 64.12
Transpotation Cost - 0.03 0.70 1.45 1.73 1.90 2.43 2.55 2.68 2.81 2.95 19.24
Other Misc. Cost - 0.00 0.08 0.16 0.19 0.21 0.27 0.28 0.30 0.31 0.33 2.14
Marketing & Publicity Cost - 0.93 23.43 50.14 62.59 71.76 92.14 98.59 105.49 112.87 120.77 738.71
Royalty - 0.19 4.69 10.03 12.52 14.35 18.43 19.72 21.10 22.5 24.15 147.74
7
Total Cost of Production (B) - 6.58 160.83 332.82 402.06 446.40 570.51 601.62 634.46 669.14 705.75 4,530.16
PBDIT (A-B) - 2.68 73.48 168.60 223.87 271.19 350.87 384.26 420.43 459.60 501.99 2,856.98
Interest
On Term Loan - 1.19 1.84 2.07 2.07 1.86 1.45 1.03 0.62 0.21 - 12.34
On Working Capital Loan - 0.13 3.31 7.01 8.68 9.87 12.66 13.50 14.39 15.34 16.36 101.25
PBDT - 1.36 68.33 159.52 213.12 259.45 336.77 369.73 405.43 444.05 485.64 2,743.40
Depreciation 0.35 0.65 0.57 0.50 0.44 0.38 0.33 0.29 0.26 0.23 0.20 4.19
Amortisation 1.72 3.86 5.15 5.15 5.15 3.43 1.29 - - - - 25.74
PBT (2.07) (3.15) 62.61 153.87 207.54 255.64 335.15 369.44 405.17 443.83 485.44 2,713.46
MAT @ 32.445% 20.31 49.92 67.34 82.94 108.74 119.86 131.46 144.00 157.50 882.08
PAT (2.07) (3.15) 42.30 103.95 140.20 172.70 226.41 249.58 273.71 299.83 327.94 1,831.39
Break Even Analysis
(Millions USD)
YEAR
1st 2nd 3rd 4th 5th 6th 7th 8th 9th 10th 11th Total
Sales Revenue (A) - 9.26 234.31 501.42 625.93 717.59 921.38 985.88 1,054.89 1,128.74 1,207.75 7,387.14
Total Cost of Production - 6.58 160.83 332.82 402.06 446.40 570.51 601.62 634.46 669.14 705.75 4,530.16
(B)
Contribution towards - 2.68 73.48 168.60 223.87 271.19 350.87 384.26 420.43 459.60 501.99 2,856.98
fixed
cost (A)-(B)
Fixed Cost
Interest - 1.32 5.15 9.08 10.75 11.73 14.11 14.53 15.01 15.55 16.36 113.59
Depreciation 0.65 0.57 0.50 0.44 0.38 0.33 0.29 0.26 0.23 0.20 4.19
0.35
Amortisation 3.86 5.15 5.15 5.15 3.43 1.29 - - - - 25.74
1.72
Wages & Salaries(25% of - 0.00 0.08 0.16 0.19 0.21 0.27 0.28 0.30 0.31 0.33 2.14
total)
Administrative Cost(30% - 0.02 0.59 1.20 1.44 1.59 2.02 2.13 2.23 2.34 2.46 16.03
of total)
Marketing & Publicity - 0.09 2.34 5.01 6.26 7.18 9.21 9.86 10.55 11.29 12.08 73.87
Cost(10% of total)
Total (C) 5.95 13.88 21.11 24.23 24.52 27.24 27.09 28.34 29.72 31.42 33.44
2.07
Cash Flow Statement
S. No. Description (Millions USD)
YEAR
1st 2nd 3rd 4th 5th 6th 7th 8th 9th 10th 11th
A Sources
1 Increase in Share Capital
Equity - - -
2 Long Term Loan 10.87 12.25 6.43 - - - - - - - -
3 Working Capital Loan - 1.89 45.37 52.96 23.81 16.99 39.83 11.95 12.75 13.61 14.52
4 Cash Earned Before Interest - 2.68 73.48 168.60 223.87 271.19 350.87 384.26 420.43 459.60 501.99
Total 10.87 16.82 125.28 221.56 247.68 288.18 390.71 396.21 433.19 473.21 516.51
B Application
1 Market devlopment 10.87 12.25 6.43 - -
Expenditure
2 Increase in Working Capital - 1.89 45.37 52.96 23.81 16.99 39.83 11.95 12.75 13.61 14.52
3 Interest
- Term Loan - 1.19 1.84 2.07 2.07 1.86 1.45 1.03 0.62 0.21 -
- Working Capital - 0.13 3.31 7.01 8.68 9.87 12.66 13.50 14.39 15.34 16.36
4 Repayment of Term Loan - - - - - 5.91 5.91 5.91 5.91 5.91 -
5 Repayment of WC Loan
6 Tax - - 20.31 49.92 67.34 82.94 108.74 119.86 131.46 144.00 157.50
Total 10.87 15.45 77.26 111.96 101.89 117.58 168.59 152.25 165.13 179.06 188.38
Net Cash Balance - 1.36 48.01 109.59 145.79 170.60 222.12 243.96 268.06 294.14 328.14
Total Cash - 1.36 49.37 158.97 304.76 475.36 697.47 941.43 1,209.49 1,503.64 1,831.78
Total Cash & cash Bank Bal. - 1.36 49.37 158.97 304.76 475.36 697.47 941.43 1,209.49 1,503.64 1,831.78
0.00 1.36 49.37 158.97 304.76 475.36 697.47 941.43 1209.49 1503.64 1831.78
Balance Sheet
(Million USD)
YEAR
1st 2nd 3rd 4th 5th 6th 7th 8th 9th 10th 11th
Liabilities
Share Capital
Equity 4.57 4.57 4.57 4.57 4.57 4.57 4.57 4.57 4.57 4.57
4.57
Reserves & Surplus (2.07) (5.22) 37.08 141.02 281.23 453.93 680.33 929.91 1,203.62 1,503.45 1,831.39
Shareholders Fund 2.51 (0.65) 41.65 145.60 285.80 458.50 684.91 934.48 1,208.20 1,508.02 1,835.96
Long Term Loan 10.87 23.12 29.54 29.54 29.54 23.64 17.73 11.82 5.91 - -
Working Capital Loan - 1.89 47.25 100.21 124.02 141.01 180.84 192.79 205.55 219.15 233.67
Current Liabilities
Creditors - 0.28 6.84 14.04 16.80 18.49 23.60 24.78 26.02 27.32 28.68
Expenses Payable - 0.08 2.11 4.50 5.60 6.40 8.21 8.77 9.37 10.01 10.70
Statutory Liabilities
Total Liabilities 13.38 24.72 127.40 293.89 461.77 648.03 915.29 1,172.65 1,455.04 1,764.51 2,109.02
Assets
Fixed Assets
Gross Assets 5.67 7.21 7.21 7.21 7.21 7.21 7.21 7.21 7.21 7.21 7.21
Less: Depreciation 0.35 1.00 1.56 2.06 2.50 2.88 3.21 3.51 3.77 3.99 4.19
Net Assets 5.33 6.21 5.64 5.14 4.71 4.33 3.99 3.70 3.44 3.21 3.02
Contingent Funds 1.17 1.17 1.17 1.17 1.17 1.17 1.17 1.17 1.17 1.17 1.17
Preliminary Ex. Not written off 6.88 13.73 15.01 9.86 4.71 1.29 (0.00) (0.00) (0.00) (0.00) (0.00)
Current Assets
Inventories - 0.73 17.69 36.32 43.53 47.94 61.19 64.28 67.53 70.94 74.52
Debtors - 1.52 38.52 82.42 102.89 117.96 151.46 162.06 173.41 185.55 198.53
Presentation by