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toxins, chemotherapeutic agents, and the like. sertate comprising a sequence complementary of
The monoclonal antibody secreted by the said specific sequence of nucleotides. Said probe
hybridoma is a valuable reagent for research and is characterized in that at least one portion of at
diagnosis. least one of the constituent nucleotides of its
nucleic acid is replaced by modified bases
recognizable by the polymerasis and comprising
a group having not more than two atoms of car-
bon and, preferably, at least one halogen group.
4529713

RADIOACTIVE STILBENE
DERIVATIVES IN 8502467
RADIOIMMUNOASSAY
PURIFICATION OF CANCER-
Alain Jouquey, Gaeta Touyer, Paris, France as- ASSOCIATED PROTEIN AND
signed to Roussel Uclaf PREPARATION OF ANTIBODY
THERETO

Thomas E WEBB, Dorothy E SCHUMM, Mar-

garet HANAUSEK-WALASZEK assigned to
THE OHIO STATE UNIVERSITY
RESEARCH FOUNDATION

It has previously been shown that the serum

Novel radioactive stilbene derivatives marked
with iodine 125 or 131 of the formula See Patent
forChemical Structure I wherein each A and B
are both hydrogen or together form a double
bond and R is the remainder of amino acid of the
formula R-NH2 or a derivative thereof poss-
essing an iodine acceptor group and marked with
iodine 125 or 13 1 and their preparation and anti-
gens obtained therefrom and a process for pre-
paring said antigens.
8502415
NON RADIOACTIVE PROBE AND
PROCESS FOR THE
CHARACTERIZATION OF A
DTERMINED NUCLEOTIDE
SEQUENCE AND MEANS FOR
THE PRODUCTION OF SAID NON
RADIOACTIVE PROBE
from patients suffering from a wide range of
cancers contains a cancer marker protein having
the ability to release RNA from cell nuclei. This
cancer marker protein is purified by taking the
protein fraction precipitating between 300/, and
50% saturated aqueous ammonium sulfate solu-
tion, dialyzing a solution of the protein fraction
against TMK buffer, chromatographing the dia-
lyzed protein on a molecular sieve and isolating
the fraction having a molecular weight of about
60,000; and removing albumin. Injection of the
purified protein into rabbits, preparation of
serum from blood of the rabbits and absorption
of the sera with normal plasma fraction produces
an antibody specific to the cancer marker protein
and therefore useful in a radioimmunoassay or
ELISA test for a wide variety of cancers. Mono-
clonal antibody preparations to the protein may
be prepared by injecting the purified protein into
mice, harvesting the spleen and lymph node cells,
fusing these cells with mouse myeloma cells and
using the resultant hybridoma cells to produce
the monoclonal antibody.

Stratis AVRAMEAS, Antoine DANCHIN,

8502604
Th@r@se TERNYNCK, Fran@ois
TRAINCARD, 1, rue Sarasate, F-75015 Paris,
France assigned to INSTITUT PASTEUR; IN- PREPARATION OF
STITUT NATIONAL DE LA RECHERCHE RADIOPHARMACEUTICALS
SCIENTIFIQUE BASED ON TECHNETIUM-WM/

Non radioactive probe for the detection by Meir LICHTENSTEIN, Nouria Faze1
hybridization of a determined sequence of SALEHI, Peter Michael POJER, 17 Yeneda
nucleotides and comprising as an essential con- Street, North Balwyn, VIC 3104, Australia as-
stituent a nucleic acid which itself contains an in- signed to THE ROYAL MELBOURNE