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What is deference between TLC and Chromatography?

Thin-layer chromatography (TLC) is a chromatography technique used


to separate non-volatile mixtures. Thin-layer chromatography is
performed on a sheet of glass, plastic, or aluminium foil, which is coated
with a thin layer of adsorbent material, usually silica gel, aluminium oxide
(alumina), or cellulose. This layer of adsorbent is known as the
stationary phase.
Paper chromatography is an analytical method used to separate
coloured chemicals or substances, it is primarily used as a teaching tool,
A paper chromatography variant, two-dimensional chromatography
involves using two solvents and rotating the paper 90° in between
The difference between Thin-layer chromatography and Paper
chromatography is abbreviated in below schedule,

THIN LAYER CHROMATOGRAPHY PAPER CHROMATOGRAPHY


Here paper is generally used whatman
in TLC, chromatoplates are prepared by
filter paper. Silica gel or alumina cannot
coating thin layers of silica gel or alumina on
be used because they cannot be made
glass plates.
into sheets.
in TLC, corrosive reagents may be coated on Here, corrosive reagents destroy the
glass plates. paper
The principle of separation is adsorption The principle of separation is partition
More amount of substance is required. Less amount of substance is required.
Less time consuming i.e. 15–45 minutes More time consuming i.e. 2–3 hours
TLC plates can be heated in an oven for a Paper cannot be heated in an oven for
long time a long time.
Separation is more sharp Separation is less sharp.
Sensitivity of detection of the fraction on
Less sensitivity of detection. Spots
plate is high. The spots are less diffused
easily diffused
Paper chromatography cannot be
TLC can be evaluated under UV.
evaluated under UV.
Due to lack of physical strength in
Thin layer have physical strength. Ascending
paper, descending techniques are
techniques are preferred for this type
used.

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