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Thin-layer chromatography (TLC) and paper chromatography are both analytical techniques used to separate mixtures. TLC involves coating a thin layer of adsorbent like silica gel or alumina onto a glass or aluminum plate, while paper chromatography uses filter paper. The mixture is spotted onto the plate or paper and moved by a solvent; different compounds travel different distances based on how strongly they adhere to the stationary and mobile phases. TLC allows for sharper separation, uses less of the substance, and is less time consuming than paper chromatography.
Thin-layer chromatography (TLC) and paper chromatography are both analytical techniques used to separate mixtures. TLC involves coating a thin layer of adsorbent like silica gel or alumina onto a glass or aluminum plate, while paper chromatography uses filter paper. The mixture is spotted onto the plate or paper and moved by a solvent; different compounds travel different distances based on how strongly they adhere to the stationary and mobile phases. TLC allows for sharper separation, uses less of the substance, and is less time consuming than paper chromatography.
Thin-layer chromatography (TLC) and paper chromatography are both analytical techniques used to separate mixtures. TLC involves coating a thin layer of adsorbent like silica gel or alumina onto a glass or aluminum plate, while paper chromatography uses filter paper. The mixture is spotted onto the plate or paper and moved by a solvent; different compounds travel different distances based on how strongly they adhere to the stationary and mobile phases. TLC allows for sharper separation, uses less of the substance, and is less time consuming than paper chromatography.
Thin-layer chromatography (TLC) is a chromatography technique used
to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose. This layer of adsorbent is known as the stationary phase. Paper chromatography is an analytical method used to separate coloured chemicals or substances, it is primarily used as a teaching tool, A paper chromatography variant, two-dimensional chromatography involves using two solvents and rotating the paper 90° in between The difference between Thin-layer chromatography and Paper chromatography is abbreviated in below schedule,
THIN LAYER CHROMATOGRAPHY PAPER CHROMATOGRAPHY
Here paper is generally used whatman in TLC, chromatoplates are prepared by filter paper. Silica gel or alumina cannot coating thin layers of silica gel or alumina on be used because they cannot be made glass plates. into sheets. in TLC, corrosive reagents may be coated on Here, corrosive reagents destroy the glass plates. paper The principle of separation is adsorption The principle of separation is partition More amount of substance is required. Less amount of substance is required. Less time consuming i.e. 15–45 minutes More time consuming i.e. 2–3 hours TLC plates can be heated in an oven for a Paper cannot be heated in an oven for long time a long time. Separation is more sharp Separation is less sharp. Sensitivity of detection of the fraction on Less sensitivity of detection. Spots plate is high. The spots are less diffused easily diffused Paper chromatography cannot be TLC can be evaluated under UV. evaluated under UV. Due to lack of physical strength in Thin layer have physical strength. Ascending paper, descending techniques are techniques are preferred for this type used.