Capitole de curs (fisiere, locatie)

Nota= a se citi in special textul marcat cu galben

Material suplimentar (carti, optional)=

1.- Rajiv Dutta, Fundamentals of biochemical engineering, Springer, Berlin, 2008
2.- Maria, G., Analiza statistica si corelarea datelor experimentale (bio)chimice. Repartitii si
estimatori statistici, Ed. Printech, Bucharest, 2008 (550 pag.), ISBN 978-973-718-886-1.

Cap. 1.
Etapele procesului biotehnologic
FISA_tehnologii-biochimice- g-maria-2018.doc (cap. 8.1)
Avantaje proc. Enzimatice din=
INTRO / intro-2.pdf

Enzime
FLUXOMICA / Inginerie metabolica_1.pdf (slides 7-12,16,17,28)
INTRO / proiectare-proc-enzimatice-2.pdf (slides 2-9)
INTRO / enzime-1.doc

Enzime – purificare (liza mecanica sau criogenica; extragere si separare cu solventi pe

coloane cromatografice)

IMOBILIZARE ENZIME / imobilizare enzime-luta-2.ppt (slide 3)

Enzime – imobilizare
IMOBILIZARE ENZIME /
imobilizare enzime-luta-2.ppt (slide 3)
imobilizare enzime-mara-1.doc
Immobilized Enzymes.ppt
Górecka-Immob-enz-review.pdf

Cap. 2. Cinetica enzimatica

MODELE CINETICE ENZIMATICE

Inginerie metabolica_1.ppt (slide 9-12, 86-105)
(expresie Michaelis-Menten, modele cinetica cu inhibitie, diagrama Eadie)

Cap. 3. Fluxomica metabolica celulara- obtinere enzime din OMG (micro-organisme /

bacterii modificate genetic)

FLUXOMICA /
Inginerie metabolica_1.pdf (slides 16-18,28-34, 55-62)
Ing-Metab-prez-curs-2017f.pdf (slides 10-11, 14, 30, 34-36)
Ing Metabolica prezenatare curs POSDRU.ppt (slides 7-10, 22-29)
3 metode obtinere GMO pe baza analizei fluxurilor metabolice (viteze de reactie enzimatice
celulare in regim de crestere echilibrata, stationara, homeostaza):
1) Eliminare gena/gene
2) Inlocuire gena tinta cu alta preluata din alt organism
3) Crestere concentratie gena tinta prin adaugare de plasmide; se va obtine o supra-
expresie gena tinta, adica o conc. mai mare enzima tinta

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 Fig. 5. The prokaryote cell structure. Fig. 6. The direct link between genome and the
(source= metabolic fluxes of the cell (Noble, 2006[45];
https://en.wikipedia.org/wiki/File:Average_prok Alberts et al., 2002[39]).
aryote_cell-_en.svg )

Fig. 99. Examples of detailed gene–protein–reaction (GPR) associations. (1) Simple

association, in which a single gene encodes a single enzyme. (2) Isozymes, in which
multiple genes encode distinct proteins carrying out the same function.
(3)Multimeric protein complex, wherein multiple genes encoding distinct protein
subunits come together to form an active enzyme. (4) Multifunctional protein, in
which a single protein can carry out multiple reactions (adapted from Mao et al.,
2015[177])

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 Fig. 9. Some usual biological methods to alter Fig. 10. Problem to be solved when applying in-
the cell genome (Alberts et al.,2002[39]). silico design of GMO.

Fig. 7. A simple example of a design problem to
re-configure the metabolic pathway for Phenyl-
alanine synthesis (Hatzimanikatis et al.
1996[38])
Fig. 8. (Continuation). The resulted Pareto-
optimal front (of two contrary objectives, i.e.
max. selectivity in Phenyl-alanine, and min.
deviations in metabolites stationary
concentrations) is leading to two alternative
metabolic configurations.

Cap. 4. PRELUCRARE STATISTICA DATE EXPERIMENTALE BIOCHIMICE (estimare

constante Michaelis-Menten din date cinetice enzimatice)

PLANIFICARE EXPERIENTE /
est-cap8a-bis.doc
est-cap8b-bis.doc

Transformare de variabila experimentala independenta=

Z I  [ Zi,min ; Zi,max ] variabila originala

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in care:

z max
j  z min
j
z oj  , (punct central al experimentului, sau nivel de baza)
2

z max
j  z min
j
z j  , (interval de variatie).
2

z j  z oj
xj  , j  1,..., k ;
z j

Xi ---. (-1, 0, +1) variabila redusa adimensionala

Repartitie normala=
Variabile aleatoare-si normala1.doc
Variabile aleatoare-si normala-2.doc

Eroare experimentala si regresie liniara=

EROARE EXPERIMENTALA
matrix-error-p-128.pdf
repartitii empirice.doc
regresia liniara=
EADIE / CALITATE MODEL DE REGRESIE / est-cap9a.doc

maria-carte-est-p-298-398.pdf (material in plus privind teste calitate model de corelatie)

exemplu la p. 364
ESTIMATORI EMPIRICI / estimatori cinetici empirici.pdf

Exemplu estimare constante Michaelis-Menten

EADIE / eadie-diagram.pdf /
MODELE CINETICE ENZIMATICE
Inginerie metabolica_1.ppt (slide 97-99)

Cap. 5. MODELE REACTOARE BIOCHIMICE

- principalele tipuri de reactoare enzimatice (constructie, mod de operare)

- dimensionare reactor pe baza cineticii enzimatice
REACTOARE / alegere reaqctoare bio-chimice-4.pdf (slide 10-13, 47-51)
REACTOARE / bioreactoare-clasificare-luta-1.doc
REACTOARE / proiectare-proc-rnzimatice-2-compressed.pdf (slides 4-7,10-11,si restul)
- ECUATII CARACTERISTICE reactoare enzimatice
REACTOARE / tipuri bioreactoare.doc / intro-clasificare.doc / gm-v4-tables.doc

Cap. 6. BAZE DE DATE BIOCHIMICE / CELULARE utilizate in estimarea cineticii enzimatice,

a conditiilor de operare bioproces, in proiectarea de enzime optimizate, in alegerea enzimei
potrivite, etc.
Ing-Metab-prez-curs-2017f.pdf (slides 22, 30-34)

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CURS 7. doua lucrari de control, inchidere situatie