'US 2008004545841 cu») United States cz) Patent Application Publication Stewart et al. (54) PARALYTIC PEPTIDE FOR USE IN NEUROMUSCULAR THERAPY (75) Inventors: John M. Stewart, Sackville (CA): Bradley J. Steeves, Woodstock (CA), Karl Vernes, Anuidale (AU) Comespondence Address: SYNNESIVEDT & LECHNER, LLP 1101 MARKET STREET 26TH FLOO PHILADEL IIA, PA 19107-2980 (US) BIOPROSPECTING NB INC, Brunswick (CA) (03) Assignee: New (21) Appl. Now 107884,871 (2) Filed Aug. 7, 2007 Related U.S. Application Data (63) Continuation of application No. 11/507, 128, filed on Aug. 21, 2006, now Pat, No, 7,273,850, whieh is a continuation of application No, 10)858,233, filed on uu, 1, 2004, now Pat. No, 7,119,168, which is a contindation-in-part of application No. 10/716,314 filed on Nov. 18, 2003, ao) Pub, No.: US 2008/0045458 Al (43) Pub. Date: Feb. 21, 2008 (60) Provisional aplication No, 601427,682, ile on Nov 18, 2002, GI) Ime. AGIK 38/16 (2006.01) A6IP 2100 (200601) 4300 2006.01) 21m (206.01) iam (2006.01) 7706 (2006.01), 708 (2006.01) 2) 4/12; $301324; 5300325; 530/326; 5301327; 530/328, 536123.5 ABSTRACT The invention relates to alow molecular weight peptide (or suite of related peptides) isolated fom the submaxiliary saliva glands of sheews ofthe species Blarina as a paralytic ‘agent. This novel paralytic agent is useful as a neuromus- colar blocker and analgesie or as an insecticide.Patent Application Publication Feb. 21,2008 Sheet 1 of 16 US 2008/0045458 AI DCSQDCAACS ILARPAELNT ETCILECEGK LSSNDTEGGL CKEFLHPSKV DLPR Figure 1A DCSQDCAACS ILARPAELNT ETCILECAGK LSSNDTEGGL CKEFLHPSKV DLPR Figure 1BPatent Application Publication Feb. 21,2008 Sheet 20f 16 US 2008/0045458 AI 1.25 2.600 kDa 1.00 E & & ozs: Q s Active fractions oso. 2 6kDa 0.28. i : { 0.00. R © 10 20 30 40 80 60 70 80 g0100 Fraction # Figure 2 Figure 3