3000 Evolution: Service Manual

3000 Evolution 
SERVICE MANUAL
610069_2.doc
BIOCHEMICAL SYSTEMS INTERNATIONAL

3000 Evolution Service Manual
© 2005 Biochemical Systems International srl . All Rights Reserved.

The information contained in this manual is subject to change without notice at any time. The
collection and verification of all documentation has been carried out with due care; however,
B.S.I. is not responsible for the utilization of this documentation.
Biochemical Systems International srl

Via G. Ferraris, 220
52100 AREZZO - ITALY
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TABLE OF CONTENTS
1. INTRODUCTION .........................................................................................................................................5
1.1 INSTRUMENT’S DEVICES DESCRIPTION.............................................................................6
1.2 3000 Evolution - GENERAL DESCRIPTION and Block Diagram.............................................9
1.3 How to change the voltage according to the country......................................................................11
1.4 Power supply and mother board description ..................................................................................11
2.TECHNICAL CHARACTERISTICS.............................................................................................................12
3.TEST AND UTILITY PROGRAMS..............................................................................................................13
Printer Interface Test .............................................................................................................14
ABS Mode.............................................................................................................................14
Pump Calibration program.....................................................................................................15
Diagnostic & Service.............................................................................................................16
Service only...........................................................................................................................17
Host programming:................................................................................................................17
Hardware Test .......................................................................................................................17
Setup program .......................................................................................................................19
4. REPLACEMENTS AND ADJUSTAMENT.................................................................................................20
BATTERY REPLACEMENT...............................................................................................20
LAMP REPLACEMENT AND CENTERING .....................................................................20
DARK CURRENT SETTING...............................................................................................22
FILTER CHECK ...................................................................................................................23
PREAMPLIFIER BOARD GAIN ADJUSTMENT ..............................................................24
FILTERS REPLACEMENT..................................................................................................26
CPU BOARD ADJUSTMENT .............................................................................................28
PERISTALTIC PUMP TUBE REPLACEMENT/MANTEINANCE ...................................29
ADJUSTMENT OF TEMPERATURE MEASUREMENT ..................................................31
CHANGING HEATING ELEMENTS..................................................................................33
5. INSTRUMENT REPROGRAMMING .........................................................................................................35
REPROGRAMMING THE MASTER ..................................................................................35
REPROGRAMMING METHODICS....................................................................................35
REPROGRAMMING HEADER AND METHOD ...............................................................35
6. LIST OF SPARE PARTS ..............................................................................................................................37
APPENDIX 1: motherboard hardware CHECKPOINTS ....................................................................38
APPENDIX 2: TROUBLESHOOTING..............................................................................................42
APPENDIX 3: REDUCING CARRY-OVER AND WORKING VOLUME USING AIR-
GAP.....................................................................................................................................................44
APPENDIX 4: READING IN STANDARD CUVETTE WITH LESS THAN 1500 µL...................45
Appendix 5: How to lock methods.......................................................................................................46
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Release History
Release Date Modifications and Upgrades
_1 09/01/2006 Inserted description for command “6” in hidden commands section of
hardware test section
_2 11/01/2006 Inserted Appendix 5. Corrected explanations of key “p” and “P” in Hardwre
test section
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1. INTRODUCTION
The 3000 Evolution is an interferential filter photometer, completely managed by microprocessors. It
performs photometric measurements and processes them according to programs with parameters that
can be entered by the operator. It executes, in a rapid and precise manner, most of important
chemistry and hematology tests. Particularly, the following determinations can be carried out:
ABSORBANCE
END-POINT
KINETICS
FIXED-TIME
MULTISTANDARD
DIFFERENTIAL
The instrument is equipped with a two-way flow cell system; it ensures low carry-over values even
with limited sample volumes. Disposable macro and micro cuvettes (glass or plastic), with an optical
path of 1 cm, can be used by simply removing the flow cell from the reading compartment and placing
it on the right-side one. Seven filters (and one additional empty position) are included in the
instrument. The selection of the interferential filter is automatic, with powered handling managed by a
microprocessor. This feature makes reading easier and eliminates filter selection errors that may
occur in instruments which have a manual selection.
The optical part is very sophisticated: it consists of a high-power halogen lamp (20 W) whose light
beam is centered by a quartz lens, thus allowing a high accuracy in measurements even when
reduced-volume cuvettes are used.
A 10-position dry incubator which can contain both square and cylindrical cuvettes allow the sample
incubation before reading. The temperature of the incubator is equal to the one of the reading cell and
is selectable from 20°C to 40 °C.
The execution of the analyses and instrument programming are simple and performed by means of a
keyboard, following the instructions shown on the display. This display also shows the status and
error/fault messages. The analytical results are directly displayed in the measuring units selected by
the current program. The language of instructions can be selected between English and other
customizzable languages. The instrument is provided with a 24-columns thermal printer that can print
analytical results as well as program parameters (multistandard and kinetics results are also available
in a graphic plot). All printed information is sent to a serial RS-232 standard output. The printer can be
also completely disconnected as well as can be disabled the graphic plot.
An advanced software guides and controls all operations carried out by the user. An acoustic signal
further helps the user, by emitting a sound of a different tone from the usual one in case you pushed a
wrong key.
A hundred and twenty programs can be stored. To carry out an analysis, it is important to enter all the
parameters correctly, including the K and standard values where necessary.
The instrument is supplied already programmed. Anyway, it is advisable to check that the entered
parameter values correspond to those stated in the methods.
A modification of the analytical parameters can be done by the operator before carrying out the
analysis.
The instrument has an internal memory that can store up to 400 result. Tests are automatically stored
in memory every time they are executed. Results can be recalled in every moment in the right section
of the program manager. In this way the instrument can printout result as a batch or a profile analyzer.
The instrument is provided with two level QC program, avalaible for 30 indipendent tests.
All 3000 Evolution are subjected to a double quality control, photometric and clinical-chemical.
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1.1 INSTRUMENT’S DEVICES DESCRIPTION
Keyboard. The keyboard (Figure 3) is used in a functional way, for moving inside the Menu. Data
entering is guided by the emission of a beep when a key is pressed. The keyboard consists of 8 keys,
4 of which are arrow, for moving inside of the Menu and 4 functional. Two keys (ENTER/YES and
STOP/NO) are both functional for two kind of operation. The following table shows which functions can
be entered by pressing each of the functional keys:
Keypad KEYS PS/2 Keyboard Function

MENU
Tab ↔ To enter inside the MENU
ENTER/YES:
Enter ↵ Store the entered values. Confirm.
STOP/NO Interruption of the operations in progress. (always active
Esc
key).
WASH Washing the flow cell. Connects the peristaltic pump
----- continuously. This key functions only when the instrument is
on the main menu.
⇒ Right movement arrow
⇐ Left movement arrow
⇓ Down movement arrow
⇑ Up movement arrow
Be careful in the case you open the instrument for operation because of possible electric
shocks
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Display. It is graphic 240x128 pixel back-illuminated liquid crystal type, consisting of two 16-character
lines. Usually, the first line displays the main parameters of the analysis in progress (analysis number,
item and K). The second line guides the operator in performing the analysis in an interactive way.
Furthermore, the instrumental status and error/fault messages are also displayed.
Printer . It is a 24-columns thermal type. It can be enabled or disconnected by the operator and can
print analytical results and parameters. In multistandard and kinetic analysis it can also plot a graphic
of the results. The operating voltage range for driving the head and motor is from 23 V to 25 V. Under
the printer there is the interface board which contains the control circuit, his operating voltage range
is from 4.75 V to 5.25 V. This circuit perform a software and hardware control to detect abnormal
temperatures, position of the head-up (when head is in the up position the circuit does not activate the
heat element) and when paper runs out.
Incubator chamber. It consists of a single thermostated aluminum block with 9 +1 housings for
cylindrical and prismatic cuvettes, arranged on three rows of three, plus another position on the right
side of the reading cell. Thermostating is obtained by means of semiconductor devices which ensure
an accurate regulation of the temperature. The two housings, separated from the previous ones, are: a
reading cell compartment (on the left) and the another thermostated compartment where to place the
flow cell when another type of cuvette is used for reading. Peltier cell is an element build with two
different kind of metal soldered each other. In accordance with the Peltier effect a current through
these metals generate a difference of temperature proportional to his intensity. So if one surface of the
cell is cooling, the other surface is heating. Other two housings, separated from the previous ones are:
a reading cell compartment (on the left) and an empty compartment which contain to place the flow
cell when another type of cuvette is used for reading.
Between the reading compartment and the 10 housing for cuvettes there is the temperature sensor IC,
it is inserted in a hole fill with thermally-conductive epoxy to ensure a good thermal contact.
The reading compartment is central between the lamp from a side and the filter selector and
preamplifier board on the other side.
In the side of the lamp there is a lens to agree the light in the optical path through the reading
compartment, from the other side there is a small glass to avoid that the dust dirty the filter.
Filter selector and preamplifier board. Connected by a bracket with the incubator it is constituted by
the motor which makes the filter wheel turn around. On the wheel there is also an Hall sensor for
signaling of the filters position. Inside the same box there is the preamplifier board where the two
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trimmers RV0 and RV1 are located, RV0 is for the offset calibration and RV1 for adjustment of the
amplification, on which it is possible to act through the holes present on the right side of the bracket.
(Refer to chapter 4 for further details).
Aspiration spout. A teflon tube which protrudes from the front panel of the instrument and intakes the
sample into the flow cell.
Sample lever (Push-button). Situated under the aspiration spout. When pressed, it turns on the
peristaltic pump to intake the sample.
Flow cell. The instrument can read either in flow cell or in standard cuvette. Instrument is equipped
with 18 µL flow cell. Pay attention to the polarity of the flow cell when you insert it in the reading
compartment. Be sure that the face with the white arrow is towards the operator.
The inclination of the flow cell inside the reading compartment is optimal since it ensures that no air
bubbles are formed inside the cell itself.
CLEANING OF FLOW CELL:
To clean the cell inside, press WASH key aspirating sodium hypochlorite (10% diluited) and air. In case
this is not enough, you can use a deproteinzer such as Perchloric acid (0.33 molar) in order to remove
residual and incrostation.
A diluted solution of sodium hypochlorite (10 %) or a liquid detergent for glassware is recommended to
clean the flow cell outside.
WARNING Pay attention do not take off the flow cell by the tubes, you could break the cell in the top.
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1.2 3000 EVOLUTION - GENERAL DESCRIPTION AND BLOCK DIAGRAM
The instrument is connected to the main power supply (220 or 110, autosensing) by a cable with two
plugs, one side of the cable must be inserted into a main socket the other side in the socket of the
polysnap.
In the polysnap are located two fuses for each phase, with a switch for line and neutral. The power
supply is ten leaded to the autosensing switching power supply for delivering the right tension in the
connector J1 (refer to check points for the tension values).
Note that the switching power supply needs the motherboard and a loads to work properly (otherwise
it will oscillates at the frequency of about 1 Hz).
The mother board of the 3000 Evolution contains the circuits which carry out most functions of the
instrument, except the printer interface, that is handled by a separate board.
The mother board supplies and controls all instrument’s devices and sensors.
Block diagram
Display 240x128 Pump motor
Power supply
Motherboard
RS-232 / PS-2
Optics:
Fans controlling
Filter motor
Lamp controller
Preamp board
Thermostate ctlr
Printer
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Instrument back side:
In the back side of the instrument you can find the following item:
❷ RS-232 D-type 9-pin male connector for connection with an external serial printer or PC through
the serial port. Refer to the Appendix C of this manual for the serial transmission protocol used by
the instrument to output results. You can use Biochemical Systems International software to
connect the instrument to a PC: ask your dealer for further details.
❷ PS2 connector to connect a keyboard to 3000 Evolution
❷ Serial number of the instrument
❷ ON/OFF switch to turn ON and OFF the instrument.
❷ Cooling fan.
❷ Fuse holder, containing number 2 fuses. Refer to technical specification paragraph for fuses
values.
• Waste connector: attach here the waste tank pipe.
Figure 4: View of the back side of the instrument
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1.3 HOW TO CHANGE THE VOLTAGE ACCORDING TO THE COUNTRY

The instrument is equipped with an autosensing power supply, there si NO NEED OF REGULATION
between 110V and 220V, 50 and 60 Hz indipendently.
1.4 POWER SUPPLY AND MOTHER BOARD DESCRIPTION

The master motherboard can operate with two 24K bytes EEPROM chips, allowing QC data, result
memory and methods setting to be stored inside.
Temperature calibration is achivied by software.

Display contrast and other calibration are achivied by software.
In the motherboard there are only 2 trimmers that are required for “Out of range” and “Log calibration”,
named RV1 and RV2 and are needed to eliminate the offset of the operational amplifiers, this means
that the value of the measure of the light is shifted by this regulation.
This trimmer are generally set in the factory and never needs to be chanaged or moved, because they
are only related to the motherboard, and not to the preamplifier board or system performance and
regulations.
ASTEC LQ153 Motherboard

Power Supply
Block Diagram of the Power Supply area
In the incubator there are 2 Peltier cells, driven in parallel for thermoregulation by the microprocessor.
The Peltier cells work like a heater or cooler in accordance with the direction of the current, so they
are connected through a H-Bridge (Q11,Q12,Q15,Q16) that can change the direction of the current
that passes through the Peltier in accordance with the MCU control signal.
There are 2 LEDs in the mother board for a visual signaling, if the voltage across the Peltier cells is in
positive or in negative ( when the cells are working as coolers the green LED (LD4) is on, when they
are working as heaters the red LED (LD5) comes on).
When both the LED are off, this means no voltage is present in the Peltier cells.
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2.TECHNICAL CHARACTERISTICS
Photometric system:
Light source: long life halogen lamp 20 W
Spectral field: 340 nm - 690 nm
Filter wheel: automatic by motor
Filters: 340, 405, 492, 505, 546, 578, 630, plus one empty position;
pass-band 6 nm
Detector: at solid state
Thermostatic control:
Heating element: refrigerating and heating Peltier cell
Temperature: 20 to 40 °C
Temperature precision: + 0,1 °C
Stabilizing time: 10 minutes
Thermostatic block: at 10 positions per square or round test tubes, macro and
semimicro
Flow system:
Flow cell: 80 µL two ways
Typical working volume: 500 µL
Minimum working volume: 350 µL
Carry over: lower than 1%
Aspiration: peristaltic pump with programmable sipping volume
Measuring system:
Reset: automatic
Measuring range: -0.200 +2.500 OD
Photometric linearity: + 1 % from 0 to 2.000 OD
Photometric accuracy: + 1 % from 0 to 2.000 OD
Precision: + 1 digit
Drift: lower than 0.005 O. D. per hour
Reagent volume in cuvette: 1 ml (minimum) for macro cuvette, 0.3 ml for semimicro cuvette
Reagent volume in flow cell: 0.35 ml (minimum)
Wash function: manual
Measuring method: End-Point, Kinetic, Fixed-Time, Differentials, Multistandard.
Programming and data display:

Keyboard: 8 multifuncional keys; external connection for PS-2 keyboard
Display : graphic 240x128 pixel back-illuminated
Thermal printer: built-in graphic printer with 24 columns
Continuous memory: 120 programs
Serial output: RS-232 standard
Physical characteristics:
Power supply: 220 V - 110 V, 50/60Hz
Capacity: 160 VA
Weight: 11 Kg
Dimensions: H. 35 cm - W. 34 cm - D. 24 cm
Serial transmission protocol:

The serial output, standard RS-232, uses the following transmission protocol:
38400, N, 8, 1.
Serial connection:
The output connector, standard 9 poles, is located on the rear of the instrument. The connections are
the following:
pin 2: input data.
pin 3 output data.
pin 5 reference signal.
The connection with serial input of a personal computer type I.B.M. or compatible can be effected by
a cable with direct connection of the three pins above described.
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3.TEST AND UTILITY PROGRAMS
To verify the correct functioning of all mechanical and electrical parts, the instrument has some utility
programs.
They can be selected interacting with user-friendly software of the instruments. The following picture
shows the basic layout of the display:
Date
Software Version
V 1-x.xx 18/10/04 12.35.56 Time
Temperature Window
Top Window:
shows the status of
the machine
Body Window:
shows results and
the parameter Menu window
actually selected
Error
messages
area
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Printer Interface Test

To carry out this test, switch on the instrument while pressing the paper feed button (LF). The
electronics, that controls the printer, performs a test cycle printing a complete series of characters.
Please note that in this case only the printer interface electronics is involved.
If the LF button is pressed only for few seconds, only text printing is performed.
If LF is kept pressed during the test, also the default logo (if present) will be printed to test the graphic
mode of the software.
The main service menu appears like this window (English language),
To enter the Service Menu, press MENU when you are in Main Menu, then select “SERVICE” using
DOWN arrow and press ENTER. The Body Window appears as follows:
ABS Mode
Pump Calibration
Diagnostic & Service
ABS Mode
To select ABS Mode, enter Service Menu as explained above.
The analyser will ask to you, in sequence, to select the wavelenght of the filter, the volume of the
sample to be aspirated and the temperature of the incubator. These settings can be carried out using
arrows’ buttons.
The following picture shows the Body Windows for the wavelenght setting, for the other two features
the Body Window will appear similar:
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Read filter (nm)
340
Use arrows to select the filter, then press ENTER to confirm and go to the next setting (Temperature
and aspiration volume).
On the contrary press STOP to exit from ABS mode. When you completed the settings, on the Body
Window will appear the message “INSERT BLANK”. Insert the blank cuvette into the reading cell and
press ENTER. Then will appear the message “INSERT SAMPLE”: insert the sample cuvette into the
redaing cell and press ENTER. The absorbance value will continuously be shown until STOP key is
pressed. This method, applied to sample control solutions with known absorbance value at a specific
wavelength, can be used to verify the instrument accuracy.
Pump Calibration program
The peristaltic pump is moved by a stepper motor which permit to intake the volume of sample with
high level of precision.
WARNING This calibration should be carry out periodically, because variation in the elasticity of
peristaltic silicon tube could vary the quantity actually sipped.
5 mL
Procedure:
- Select the Pump calibration program.
- Put the aspiration spout into a cuvette with 5 ml accurate of distilled water.
- Press the push button. The peristaltic will begin to work.
- When the target quantity has been aspirated (5 mL) press immediately the push button. The LCD
display the value in ms of the time that occurred. This value is stored and is used as reference for
the instrument to intake any quantity of sample. Optimal value goes from 8000 to 14000.
- The instrument will return automatically to main menu after few seconds.
Value:
less than 8000 abnormal motor running. Too fast or wrong tube.
8000- 14000 the rubber is efficent and in good condition.
Bigger than 14000 Obstruction on the aspiration spout or tube consumed.
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You can reach “Diagnostic & Service” selecting “Diagnost. & Service” in the Service Menu. The Body
Window will appear as follows:
Quick Diagnostic
Service Only
If you select “QUICK DIAGNOSTIC” the analyser will print some self-diagnostic parameters, which
colud be helpful when you call service to solve any problem.
This section will explain the meaning of the parameters printed by “Quick diagnostic” mode.
FILTER #1: 2577 Transmittance value of the filter 1 OK

FILTER #3: 965 Transmittance value of the filter 3 Too low! Energy to HIGH. Needs gain
Adjustement or coating

FILTER #5: 15500 Transmittance value of the filter 5 Too high!! Energy too low! Needs replace
Or coating removal

FILTER #8: 222 Transmittance value of the filter 8 (Empty)
CAL: 10740 Internal calibration. Range: 8000-16000

PMP: 9000 Pump calibration Volume. Range: 6000-13000
Tw: 23.2 C Flow cell and incubator temperature
Ta: 21.4 C Motherboard temperature
Fan: 30 C The fan starts working at this temperature
Lamp On = 090 s Lamp’s warming up time

Lamp Off = 006 s Delay turning off time whne lamp is idle
MotL: 1000 ms Stepping motor parameter (Should be always this value)
MotV: 2000 ms Stepping motor parameter (Should be always this value)
NLet: 04 System parameter (Should be always this value)
NMet: 120 System parameter (Should be always this value)
Timeout: 60000 System parameter (Should be always this value)
OnCond: 03750 System parameter (Should be always this value)
Amax: 00450 Maximum of absorbance value for KIN and FXT mode
IntChk: 002 System parameter (Should be always this value)
Int ok: 012 System parameter (Should be always this value)
CODelay: 003 System parameter (Should be always this value)
m = 0.049505 Temperature parameter (different for each instrument)
q = 0.049509 Temperature parameter (different for each instrument)
EEP: 3kEv_1.04 Version of EEPROM program

HDWR: 1 Hardware version
MSG: 1.05 Language messages version
SWR: 1.09 Master firmware version
SLV: 1.05 Slave firmware version
[0]: 0, 0 Linearity compensation

Pag 16 of 46
Service only
This area is reserved to service engineers.
Host programming
Hardware Test
Host programming:
Host programming is useful for transferring data to the instrument and setting up the method inside the
120 test memory (this is useful for customizing most of the method parameters, like K-Factor, test
name, standard concentration, number of decimal point and so on).
You can use this program also to restore factory settings (in case of memory corruption). You need a
special program (called HM3000.exe), running in Microsoft Windows (c), to transfer the method file
and parameters to the instrument.
Refer to chapter 5 on how to use this program to connect it with the instrument.
This program is also used to upgrade the Slave MCU (in case it is needed), by the mean of a special
program (refer to BSI website or contact BSI service engineer for further info).
This program is not suitable for upgrading the Master MCU (you MUST use, in this case, the
Bootloader, so you have to start with the instrument OFF).
Hardware Test
NOTE:
In order to work with this program you have to connect EXTERNAL PS-2 keyboard.
Otherwise you will never access to the basic hardware function of the instrument.
Be aware that matrix keyboard do not produce any command (except double click when STOP is
pressed) and WASH (to verify instrument washing), in order to make it possible to diagnostic it.
Only PS2 code are allowed in this program.
Check of matrix keyboard:

To check if the matrix keyboard is working properly you can press the key of the matrix keyboard and
compare the value displayed with the corresponding one on the table.
ENTER MENU ← ↑ → ↓ STOP WASH

10 1 10000 100K 10M 1M Exit 1K
K = 1’000
M = 1'000’000
To check if the PS2 keyboard is working it is enough to press NUM LOCK key and verify the
corresponding LED status (ON and OFF).
NOTE:
PS2 key are KEY sensitive. So it MAKES different from command capital and small
Pag 17 of 46
Other useful key in service program:
KEY FUNCTION
b Buzzer test
T Temperature calibration (Warning: special device is needed!!) DO NOT PERFORM WITHOUT
SPECIAL TOOLS
R Execute automatic and continuos reading on the selected filter
1-8 Makes filter selection:
1 = 340
2 = 405
3 = 492
4 = 505
5 = 546
6 = 578
7 = 630
8 = Empty
H Execute filter wheel homing and realignment
p Printer test
0 Test Serial port to PC
V Burn in mode for instrument (therm cycle) (TEST PROGRAM)
L Set linearity compensantion values. (Warning: special device is needed!!) DO NOT
PERFORM WITHOUT SPECIAL TOOLS
C Execute instrument calibration
D Test/Set RTC (Real Time Clock): date and time
f Test ON/OFF fan
v test ON /OFF lamp
B Execute automatic bichromatic filter cross check. (TEST PROGRAM)
c Make it possible to execute visual lamp alignment (Low emission mode)

P Enable or disenable Method lock (see Appendix 5)
NOTE (hidden commands):

There are some special program that should never be entered unless it is specified by BSI service
engineer or it is known what you are doing.
INCORRECT OPERATION MAY DAMAGE INSTRUMENT PERMANENTLY
Hidden command program.

You enter in this modality by pressing Q key.
On the screen it is not shown anything, in order to prevent accidental intrusion.
Possible commands:
1= Modifiy 'AMAX' .
2= Restore of the EEPROM and HEADER factory setting (in case of corruption of memory)
3= Temperature offset adjustment (q).
NOTE: To decrease temperature, increase the offset, to increase the temperature, decrease the offset.
Refer to chapter 4, section ADJUSMENT OF TEMPERATURE MESUREMENT
4 = Restore default language

5 = Enable temperature in status bar.
6 = Edit wavelength for optional filter
9 = Enable/disable BURN IN (DO NOT USE !!!) THE INSTRUMENT WILL STOP TO RESPOND IF BURN IN IS ENABLED.
If accidentally activate, deactivate it by following the same procedure.
Pag 18 of 46
Setup program
In this program it is possible to set the some print characteristic, the language regarding the display
message and to active the air gap choosing the quantity of air and the delay time between the sample
aspiration and air aspiration, and so on.
The air gap could be introduced to save a small quantity of sample, actually to reduce the carry-over
effect it must be aspirated a volume of sample enough to fill the flow cell and all the way from the
aspiration spout, so is possible to intake the first part with the sample to fill the flow cell and following
part with air to fill the tube.
WARNING Pay attention to set the quantity of air not too big otherwise the air enter in the flow cell.
This will lead to incorrect result and reproducibility problems.
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4. REPLACEMENTS AND ADJUSTAMENT

About the electronic there are 3 board that could be replaced.
Interface printer board  No adjustment is required.
Mother board  This board is supplied calibrated but needed to be checked the temperature control
because each temperature sensor is slightly different.
Preamplifier board  This replacement requires the calibration of the absorbance with a cuvette of
know value adjusting the trimmer R1. The trimmer R4 is for adjust the amplifier to set the value A in
the range 2500 - 7500 for all filter.
After this replacement the calibration for Hematocrit and Erythrocytes tests could be no correct, if you
want verify these test you can ask B.S.I. headquarters instructions.
PRELIMINARY PROCEDURE
Before performing the following operations, make sure that the instrument is switched off, open the
instrument unscrewing the four screws from the bottom that fix the cover on the basis and place the
cover on the right side without turning it over in order to be able to operate on the keyboard.
BATTERY REPLACEMENT
You need to replace the battery when you have a malfunctioning on date and time.
The nominal voltage value at battery pins cannot be less of 3V.
LAMP REPLACEMENT AND CENTERING

1. Perform the preliminary procedure
2. Disconnect the lamp cables from connector J4 (refer to Appendix 1) unscrewing both screws
3. Replace the lamp just unscrewing the two screws on lamp support. Pay attention not to touch
with fingers the lamp bulb. Connect the lamp cables to connector J4
4. After the change, you only need to center the lamp with the reading aperture of the
photometer, which is inside the reading hole. The following pictures shows how to perform the
centering
Reading
aperture of he
photometer
Pag 20 of 46
5. It is possible that after having centered the lamp, there is too much energy on the preamplifier
board. You need thus to perform PREAMPLIFIER BOARD GAIN ADJUSTMENT (see the related
section in this chapter).
Pag 21 of 46
DARK CURRENT SETTING
The preamp board has a bias current due to the offset of its operational amplifier. This current has to
be compensated in order to have correct results.
First of all, perform preliminary procedure to open the instrument. Then connect a multimeter to test
points TP2 and TP5 on thye mother board (see Appendix 1). These test points are directly connected
to preamp output: you have to connect the red tip of the multimeter to TP2 (OFFSET value) and the
black tip of the multimeter to TP5 (GND).
Then switch on the instrument and insert the cuvette into the reading hole. Rotate the cuvette for 90°,
in order to have the dark face of the cuvette in front of the photometer. Select the best resolution you
have on your multimeter (you need at least 0.1 mV for resolution). Act on trimmer RV0 of preamp
board (see following picture) until you reach a value between 0.5 mV and 1mV.
NORMAL ROTATED 90°
Trimmer RV0: Offset
Pag 22 of 46
FILTER CHECK
Perform “Quick Diagnostic” program as explained in chapter 3. The instrument prints out a value
that is reversely related to the transmittance values of the filters (which is the output of the A/D
converter): these values should stay in the range 1200-8000 .
In fact, if this value is less than 1200, this means that the energy values releated to the filter is too
high and the preamp board is working in saturation: the instrument can only read high absorbance
samples (which bring energy to lower values), without accuracy nor good resolution.
If the ADC output is more than 8000, this means that the energy value oh the filter is too low and
the preamp borad is working near interdiction zone, so the instrument can read correctly only
samples with low absorbance and samples with high absorbance will be underextimated.
In order to have all the values in the safe area, filters are coated rxternally with some gelatines to
make light attenutation (only 340 nm filter isn’t coated and all the other filter are equalized to this
value).
The following picture sums up the concept:
Offset subtracted to reading: can only read high absorbance values

without accuracy and resolution (Preamp board in saturation)
1200
SAFE RANGE
8000
Underextimate high absorbance values

(Preamp board in interdiction zone: values with low
absorbance values are accurate and correct)
ADC OUTPUT
Pag 23 of 46
PREAMPLIFIER BOARD GAIN ADJUSTMENT
If filters’ transmittance values are not all in the safe area, you should adjust the gain of the preamp
board (ADC converter). Enter “Hardware Test” and press “R” in PS2 keyboard to execute a continuos
reading of the transmittance values of the filters. Press “1” in PS2 keyboard to select filter one (340
nm) and check the value if it is not in the safe area (1200-8000), you have to act on trimmer RV1 of
preamplifier board until the value is not in the correct range.
Trimmer RV1:
GAIN
Repeat the operation for all the seven filters (you can select a filter pressing the corrispondent number
on the PS2 keyboard).
If for one or more filter is not possible to reach Safe Area, you need to add or remove the gelatine
coating or to replace the filter (see next pictures as examples, and next paragraph for description).
1 2 3 4 5 6 7
1200
ALL in SAFE AREA
8000
340
630
ADC Output
Pag 24 of 46
1 2 3 4 5 6 7
1200
Filter 3 is not in safe area:
remove some gelatin
coating to increase energy
value
8000
340
630
ADC Output
1 2 3 4 5 6 7
1200 Filter 3 is not in safe area, add

some gelatine coating to
decrease energy value
(gelatine will block more
photons)
8000
340 630
ADC Output
Pag 25 of 46
FILTERS REPLACEMENT
If you need to replace some filter, first of all you have to perform preliminary procedure.
Then unscrew the four screw which tie the two stirrups of dissipator block to the basement.
Disconnect all the cable of the entire block from the motherboard and remove the entire block
(dissipator + incubator) from the instrument.
Then unscrew the four screw shown in the following picture to separate filter wheel block from the
incubator.
Then rotate filter wheel by hand, until the hole on the bracket coincides with the one on the filter wheel.
Pag 26 of 46
Use a 1,5 mm spanner to loosen the grain and liberate the filter wheel. Be sure that the little washer is
coupled with the stepping motor shaft.
Washer: indispensable for a

perfect coupling between
stepping motor and filter wheel
The following picture shows the filter wheel:
Pag 27 of 46
With a screwdriver remove the fixing washer and replace the filter paying attention that the mirror face
is turned downwards, furthermore pay attention to the gelatins under the filter (for 340 nm filter no
gelatin is present). AVOID to touch the 2 optical surface of the filter and the gelatins by hands not to
soil them.
After the replacement, remount all the parts and perform preamplifier board gain adjustment.
POS Filter (nm) Equalization
1 340 Nothing
2 405 1 gel di 2 + 1 gel di 5
3 492 2 gel di 2 + 2 gel di 5
4 505 1 gel di 4 + 1 gel di 1
5 546 1 gel di 4 + 1 gel di 2
6 578 1 gel di 4 + 1 gel di 3
7 630 2 gel di 3
8 free -
Equalization to be used as starting point for filter coating. May changes according with filter transimattance,
age, lamp emission photodetector spectral response.
CPU BOARD ADJUSTMENT
The motherboard doesn’t need any hardware calibration, because they have been carried out by the
constructor. Please do not act anyway on trimmer RV1 and RV2 on motherboard: these trimmers have
been factory pre-setted and hardware calibration during the motherboard quality control process.
Pag 28 of 46
PERISTALTIC PUMP TUBE REPLACEMENT/MANTEINANCE

Perform the preliminary procedure, in order to access to the peristaltic pump tube.
You can replace peristaltic pump tube by simply detaching it from the plastic tip connected to the
TEFLON tube.
See the figure below for a schematic view of the pump:
PERISTALTIC PUMP
OUT WARP DIRECTION
TEFLON
IN
PLATE
ADAPTER BETWEEN TEFLON AND

ELASTIC TUBE
To change the tube disconnect the elastic tube from its 2 adapters, by simply pulling them out.
Then you can replace the tube and reconnect the 2 adapters.
ADAPTER BETWEEN TEFLON AND

ELASTIC TUBE
Pag 29 of 46
Before closing the cover again, be sure that the peristaltic pump elastic tube has a suitable strength.
You can easily check for this by aspirating some distilled water and see if anything drop form the
intake tip outside the instrument. No drops must be present.
PUSH
No drops present!
If you have still some drops, despite having already changed the tube, you must insert some plastic
ring (shipped with the tube) in order to increase the strength of the elastic tube.
Usually inserting one on the top and one on the bottom it is enough for adding the missed power to the
peristaltic pump. Refer to the figure below for the correct way of placing this 2 rings:
Insert here the PLASTIC RINGS for increasing the

strength of the peristaltic pump.
Usually placing 2 rings (1 on top + 1 on bottom) it is enough to make the peristaltic pump working
properly. If not so, add more 2 rings.
Remember that if you don't have the need to add this ring, don't add them to the tube, because they
increase the strength, reducing the lifetime of the peristaltic pump tube.
NOTE: You must recalibrate the peristaltic pump aspiration volume after having changed on of the
part above.
Pag 30 of 46
ADJUSTMENT OF TEMPERATURE MEASUREMENT
To perform this operation you don’t need to open the instrument, so you don ‘t have to perform
preliminary procedure: the operation will be carried out via software. Insert a thermocouple
thermometer into reading cell and swicth it on. Next picture shows the thermocouple thermometer.
CAP 37.0
THERMOCOUPLE
CUVETTE
1000 uL WATER
THERMOMETER
NOTE: Be sure that your thermocouple thermometer is calibrated and with ± 0.1 °C of accuracy in
measurements. If you set temperature at a wrong value Kinetic analysis results will be mistaken.
NOTE: If you use mercury thermometer you should insert it in a cuvette filled with water NOT
DIRECTLY IN THE INCUBATOR. You need ± 0.1 °C of accuracy in measurements. You need more
time to reach a stable temperature with a mercury thermometer, since it affects the measurement
greater than a thermocouple (typically the waiting time are doubled, i.e. 1h.)
Connect a PS-2 keyboard to the instrument and switch it on. Enter “Service Only” Menu as explained
in chapter 3 and select “Hardware Test”. Press “Q” key on PS-2 keyboard to enter hidden command
program. The display will show only the script “Press ENTER to return!”. Don’t press ENTER but press
“3” key to perform temperature offset adjustment. Press ENTER as the instrument ask you to confirm.
On the display will appear this window:
Pag 31 of 46
Increase or Decrease?
Yes=Inc. NO=Dec
You have to press “YES” or “ENTER” to increase the offset value or “NO” or “ESC” to decrease it.
Increasing offset will decrease the temperature value, decreasing offset will increase
temperature value.
Depending on your choice, the display will show one of this window:
- 0.00 + 0.00
With arrows and number keys, choose the value to increase or decrease offset value and press
ENTER. The display will show the script “>>>>> OK! <<<<<”: press ENTER again.
Wait until the temperature value on your thermocouple thermometer become stable.
Repeat the operation until the temperature value reachs 37 °C. Then exit from “Hardware Test” and
press ENTER as the instrument ask if you want to save the changes.
Pag 32 of 46
CHANGING HEATING ELEMENTS

Heating elements (Peltier Cells) are located under the incubator and fixed to the heat dissipator in two
proper lodging.
Perform the preliminary procedure to open the instrument. Then extract dissipator-incubator block as
explained in “Filters Replacement” section.
Now unscrew the four black screw which tie incubator to the dissipator. The following picture shows
two of these screw, on the opposite side you will find the others.
Then pull up the incubator: next picture will show the two blocks separated (in this picture also the
lamp has been removed)
Thermal
conductive
PAD
Peltier Cell
Peltier Cell
Replace Peltier cells according to the picture above. Remount incubator on the dissipator: you can
remove one of the two lateral stirrup to perform the operation easier. Be sure to screw the four black
screw of the incubator so that pulling peltier cables doesn’t make the cell to move.
Pag 33 of 46
The Peltier are coupled to the incubator and the heatsinker with a thermic conductive PAD. This PAD
ensure good heating transmission and increase heating and cooling efficency.
Assemble the Peltier cells in this way:
Incubator
Pink thermic
conductive
PELTIER CELL PAD
Heatsinker Grey thermic

conductive
PAD
Important:
Avoid thermal short circuit to increase heating efficency. In case there is not heating or cooling
efficency a parasistic thermal path may be present.
GOOD NO GOOD
Pag 34 of 46
5. INSTRUMENT REPROGRAMMING
This section will explain how you can reprogram the instrument
REPROGRAMMING THE MASTER

It is possible to reprogram the software of the instrument reprogramming the flash memory of Master
microprocessor. For further details contact Biochemical Systems International:
WebSite: www.biosys.it
e-mail: biochfi@biosys.it
REPROGRAMMING METHODICS
It is possible to reprogram all the methods via PC, if you need to personalize analysis parameters or if
you need to restart with default parameters.
For further details contact Biochemical Systems International:
REPROGRAMMING HEADER AND METHOD

With Header Programmer software it is possible to modify via PC the name of the instrument printed
when it is turned on and to personalize the report printed with test results in “Result Printout”
operation. You can reach “Result Printout” from “Print” Menu.
Connect the instrument to the PC through a serial cable (RS-232). Switch on the instrument and enter
“Host Programming” as explained in chapter 3.
Run Header Programmer from your PC: on your display will appear the software’s window as follows:
First of all, select the correct COM Port.

Then write the new name for the instrument (if you want to change it): 24 characters are avalaible.
Pag 35 of 46
Click “Update!” button in the Header section of the window: the counter box will count the number of
byte transferred. Wait until the transmission is complete (the counter will stand still).
To personalize the report, first of all select how many rows should it have, clicking on the arrows
buttons. Then edit the boxes you have enabled: for each row you have 24 characters avalaible.
When you are ready, click “Update!” button in Report section of the window, wait until the transmission
is complete.
Then click “Exit” button and exit from “Host Programming” menu in the instrument. Then switch off the
instrument.
For further details contact Biochemical Systems International:
Pag 36 of 46
6. LIST OF SPARE PARTS
For spare parts list, visit section “SPARE PARTS LIST” in our website www.biosys.it, and follow the
link spare parts.
Pag 37 of 46
APPENDIX 1: MOTHERBOARD HARDWARE CHECKPOINTS

This section will show all voltage values and checkpoints for 3000 Evolution motherboard:
TP2 J16
TP5
J5
J6
J9
J11
J15
J8 J3
J17
J2
J12
J18
J10 J14
J7
J4
J20
J1
Pag 38 of 46
J1: POWER SUPPLY
Pin 1: +15 V
Pin 2 : GND
Pin 3: -15 V
Pin 4: +5V
Pin 5: GND
Pin 6: NC
Pin 7: +21
Pin 8: GND
J2: External FAN (FAN 1)
Pin 1: +12V to +14 V

Pin 2: GND
J3: Incubator FAN (FAN 2)
Pin 1: +12V to 14V

Pin 2: GND
J4: LAMP
Pin 1: 10V
Pin 2: GND
J5: PHOTOMETER (PREAMP)
Pin 1: +12V
Pin 2: GND
Pin 3: -12V
Pin 4: 0V to 10V
J6: TEMPERATURE
Pin 1: +12V
Pin 2: GND
Pin 3: NC
Pin 4: Temperature (°C) = Vout (mV)/10
J7: DISPLAY BACK ILLUMINATION
Pin 1: 3V to 5V
Pin 2: GND
J8: DISPLAY PINS
Pin 1: GND
Pin 2: +5V
Pin 3: NC
Pin 4 – Pin 16: LOGIC SIGNALS
Pin 17- Pin 18: NC
Pin 19:LOGIC SIGNAL
Pin 20-Pin 21: NC
Pin 22: -17V to –10V (Contrast setting voltage)
Pin 23: GND
Pin 24: NC
J9: LED
Connector absent
Pag 39 of 46
J10: PUSH BUTTON
Pin 1: +5V normally, 0V when pressed

Pin 2: GND
J11: BUZZER
Pin 1: +5V
Pin 2: +5V normally, 0V when buzzer is on
J12: SERIAL PORT
Pin 1: NC
Pin 2: Host_TX (Standard RS-232 voltage values)
Pin 3: Host_RX (Standard RS-232 voltage values)
Pin 4: GND Float
J13: PRINTER
Pin 1: +5V
Pin 2: +5V
Pin 3: PRN_TX (Standard RS232 voltage values)
Pin 4: PRN_RX (Standard RS232 voltage values)
J14: PS-2 KEYBOARD (External)
Pin 1: +5V
Pin 2: +5V
Pin 3: GND
Pin 4: Standard PS-2 voltage values
Pin 5: Standard PS-2 voltage values
Pin 6: GND
J15: KEYBOARD
Pin 1: 0V (button pushed) 5V (button not pushed)

J16: Peristaltic Pump Motor
Pin 1: +12V
Pin 2: +12V / -12V
J17: FILTER WHEEL MOTOR
Pin 1: 0V / 5V
Pin 2: 0V / 5V
Pin 3: 0V / 5V
Pin 4: 0V / 5V
J18: HOME SENSOR FILTER WHEEL
Pin 1: +5V
Pin 2: 0V / +5V
Pin 3: GND FLOAT
Pin 4: NC
Pag 40 of 46
J19: PELTIER
Pin 1: 0V / +5V
Pin 2: 0V / +5V
Pin 3: 0V / +5V
Pin 4: 0V / +5V
J20: PELTIER POWER SUPPLY
Pin 1: +5V
Pin 2: GND
TP2: PREAMP OFFSET TEST POINT
TP5: PREAMP GROUND TEST POINT
Pag 41 of 46
APPENDIX 2: TROUBLESHOOTING
This appendix shows the error messages related to issues that the user can normally solve by himself:
if the problem persists, or a problem not listed below arise, contact your dealer.
Excluding the main plug fuses, the instrument has no user serviceable parts: only trained
technicians are allowed to service the instrument. An unauthorized action on the instrument
may invalidate its safety and features, beside void the warranty.
In case of suspect maflunctioning of the instrument, we recommend to check the instrument with
coloured solution or control serum of known value.
In the following table we describe messages and flags which appear on the Body Window or in a
window which appears in the middle of the display:
MESSAGE DESCRIPTION POSSIBLE OPERATIONS
ERROR -1 The sample absorbance is too high or the reading is Press ENTER to repeat the reading otherwise dilute
impossible, due to faulty instrument the sample. If message persists call our service
technicians.
Printer error Error when printing Press STOP to disconnect the printer, or any other
key to try again.
Temperature The thermostat is already regulated at the required

Window is fixed temperature.
Temperature The thermostat has not yet reached the required Wait.
Window is temperature.
blinking
Temperature The temperature is disenabled
Window shows
----
Const ERROR Critical error in EEPROM Call service.
EEP0 ERROR Critical error in EEPROM Call service

Delay XXX XXXX = time (in seconds) required to complete the Wait or press STOP to interrupt the analysis
analysis.
--- In a KIN or FXT analysis, the absorbance-variation of Dilute the sample.

the sample is too high during the first time-interval
(Delay).
LLLL In an MSD analysis, the sample concentration is less The sample concentration is lower than the
than that of the lowest standard. minimum range of the method.
HHHH In an MSD analysis, the sample concentration is Dilute the sample.

greater than that of the highest standard
CALIBRATE It appears when you select an EP analysis with Press ENTER if a new calibration is required. The
(Y/N) standard or FXT with standard. It allows the instrument will be ready to read the standard:
possibility to select a new calibration with the INSERT STANDARD. Press STOP if a new
standard or read directly the sample cuvette calibration is not necessary. The instrument is
ready to read the sample: INSERT SAMPLE.
D The analytical result exceeds the linearity limit of the Dilute the sample
reagents.
* The thermostat has not reached the right temperature Repeat the analysis when the thermostat and the
when the analysis was performed (blinking reagents have reached the right temperature.
temperature window). The result therefore, is not
correct.
L The analytical result is less than the lower limit value
of the method.
H The analytical result is more than the higher limit
value of the method.
In the following table, we describe Error Messages which appear in the Error Message Area
Pag 42 of 46
MESSAGE DESCRIPTION POSSIBLE OPERATION

TA ERROR Error reading internal temperature Call service
TW ERROR Error reading incubator temperature Call service
PRN PAPER The roll of printing paper is over Insert a new roll of printing paper
PRINT KO The printer is disabled or malfuncioning Disable printer or call service
PRN TEMP Overheating of LPT Wait
OVERHEATING Instrument overheating. Turn off and wait few minutes
H.FIL KO Malfunctioning of filter positioning system Call service
The instrument is provided with 2 fans. One fan is automatically controlled by microprocessor and it is
switched on and off as required, according to the internal temperature of the instrument. Internal fan is
always on and cool the incubation group.
Pag 43 of 46
APPENDIX 3: REDUCING CARRY-OVER AND WORKING VOLUME USING AIR-

GAP
Carry-over is the residual solution inside the flow cell that affects the final result of a photometric
measurement. This quantity depends by several factors, but the most important for our purpose are:
• The density of the solution used
• The absorbance value of the solution
• The internal cleanliness of the flow cell
• The quantity of aspirated volume (sample volume)
• The flow cell volume. The instrument can work either with 80 uL flow cell (that is the standard flow
cell) or with the 18 uL flow cell (upon request).
The instrument is shipped with its carry-over value indicated in the documentation that refers to its
internal Quality Control. Instrument carry-over must be less than 1% to pass the Quality Control.
To reduce instrument carry-over several measures can be taken:

• Use 18 uL flow cell instead of 80 uL flow cell.
• Wash the instrument with sodium hypoclorite to clean internally the flow cell.
• Use a greater quantity of reading solution.
• Use air-gap.
Air-gap can reduce dramatically carry-over effects, but requires a little of experience in working with it.
Air-gap is an instrument features that allows the User to set up a Sample volume, a Delay time and an
Air anspiration volume. Basically the instrument performs a DOUBLE ASPIRATION (the first for the
sample, the second for the air).
In fact, after each sample the peristaltic pump is turned OFF for a programmable amount of time (User
should remove the reading solution from the aspiration spout during this delay) and is turned
ON again for a certain amount of time in order to aspirated a programmable volume of air.
This parameters are General Setup Parameters (not test parameters) so will remain the same for all
the test.
DELAY = 00 s
ANSP. VOL. = 00000 uL
The instrument is shipped with this 2 parameters disabled (their values are set to 0000).
Recommended value for beginner are:

DELAY = 03 s
ANSP. VOL. = 00150 uL
In this way the instrument (when a sample in flow cell is executed during a test) makes a double
aspiration described in these 3 steps:
1. Aspirate the sample volume programmed for that specified test.
2. Wait for the programmed Delay time
3. Aspirate the programmed quantity of air.
You just have to enter “Setting” in “Setup” Menu to set this 2 parameters (They are called “Air
aspiration” and “Delay”).
According to the test you are executing and to the test manufacturer you can change these
parameters in order to fit their best values.
Pag 44 of 46
APPENDIX 4: READING IN STANDARD CUVETTE WITH LESS THAN 1500 µL

The analyzer is shipped with the reading height that exactly matches the reading aperture in the flow
cell (the same height for 18 or 80 uL flow cell). In this condition the analyzer can work correctly in the
flow cell and with a minimum reading volume of 1500 uL in standard plastic cuvette (10 mm optical
path), like the ones supplied with the instrument.
Some kits however have a reading volume of less than 1500 uL (for example 1000 uL). With this kit is
no possible to work with the instrument unless:
1) Use reduce volume cuvette (still 10 mm optical path). Contact your nearest distributor for more
information.
2) Adjust the PVC screw in the incubator chamber in order to increase the reading height. In this way
you can reduce the volume according to your needs, but remember that the light beam does not
match anymore the flow cell reading aperture and you have to setup it again if you want to work
again with flow cell.
The figure below explains how to adjust the reading height (regulating the height of the PVC screw) in
order to work with flow cell or reduced volume. Refer to the following table for more information:
PVC Screw HEIGHT (h) FLOW CELL REDUCE VOLUME CUVETTE STANDARD CUVETTE
0* -Figure A - OK OK (Minimum Volume 1500 uL)
2.5 turn * -Figure B- NO OK OK
*: Note that the screw height is measured according to the turn from the down position.
PVC SCREW
NOTE:
Use maximum care when performing this operation, since an excessive strength may damage permanently the
PVC screw inside the reading incubator chamber.
Pag 45 of 46
APPENDIX 5: HOW TO LOCK METHODS

This procedure allows to lock Methods in 3000 Evolution.
Be sure that a PS2 keyboard is connected to the instrument.
In Main Menu, press MENU on the little keyboard on the instrument (or TAB on PS2 Keyboard) and select
“Service” using arrows, then press ENTER.
V 1-x.xx 18/10/04 12.35.56
MAKE A SELECTION 37°C
001- OPEN -EP Setup

002- OPEN -KIN
003- OPEN -EP Print
004- OPEN -EP
005- OPEN -KIN Service
In the SERVICE menu, select “Diagnostic & Service” and press ENTER.
ABS Mode
Pump Calibration
Than select “Service Only” and press ENTER. Finally select “Hardware Test” and press ENTER.
Press “P” in the PS2 keyboard (be sure to press “P” because “p” would start printer test), the instrument ask you
if you want to lock methods: press ENTER to confirm. The display will show four digit to insert a password:
____
Write your password using PS2 keyboard and press ENTER.

Press ESC to exit from “Hardware Test”: the instrument will ask you if you want to save changes: press ENTER
to save.
Press ESC several times to return Main menu.
Following the same procedure and inserting the correct password will allow you to unlock Methods.
Reviewed in 01/11/2006
By Biochemical Systems International
Pag 46 of 46

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3000 Evolution 

BIOCHEMICAL SYSTEMS INTERNATIONAL

© 2005 Biochemical Systems International srl . All Rights Reserved.

Biochemical Systems International srl

1.1 INSTRUMENT’S DEVICES DESCRIPTION

Keypad KEYS PS/2 Keyboard Function

⇒ Right movement arrow

⇐ Left movement arrow

⇓ Down movement arrow

CLEANING OF FLOW CELL:

1.2 3000 EVOLUTION - GENERAL DESCRIPTION AND BLOCK DIAGRAM

Display 240x128 Pump motor

Instrument back side:

❷ PS2 connector to connect a keyboard to 3000 Evolution

❷ Serial number of the instrument

❷ ON/OFF switch to turn ON and OFF the instrument.

• Waste connector: attach here the waste tank pipe.

Figure 4: View of the back side of the instrument

1.3 HOW TO CHANGE THE VOLTAGE ACCORDING TO THE COUNTRY

1.4 POWER SUPPLY AND MOTHER BOARD DESCRIPTION

Temperature calibration is achivied by software.

ASTEC LQ153 Motherboard

Block Diagram of the Power Supply area

Programming and data display:

Serial transmission protocol:

3.TEST AND UTILITY PROGRAMS

Printer Interface Test

Diagnostic & Service

Read filter (nm)

Pump Calibration program

Diagnostic & Service

FILTER #1: 2577 Transmittance value of the filter 1 OK

FILTER #4: 3127 Transmittance value of the filter 4 OK

FILTER #6: 1550 Transmittance value of the filter 6 OK

CAL: 10740 Internal calibration. Range: 8000-16000

Lamp On = 090 s Lamp’s warming up time

EEP: 3kEv_1.04 Version of EEPROM program

[0]: 0, 0 Linearity compensation

Check of matrix keyboard:

ENTER MENU ← ↑ → ↓ STOP WASH

Other useful key in service program:

c Make it possible to execute visual lamp alignment (Low emission mode)

NOTE (hidden commands):

INCORRECT OPERATION MAY DAMAGE INSTRUMENT PERMANENTLY

Hidden command program.

4 = Restore default language

4. REPLACEMENTS AND ADJUSTAMENT

LAMP REPLACEMENT AND CENTERING

DARK CURRENT SETTING

NORMAL ROTATED 90°

Trimmer RV0: Offset

Offset subtracted to reading: can only read high absorbance values

Underextimate high absorbance values

PREAMPLIFIER BOARD GAIN ADJUSTMENT

1200 Filter 3 is not in safe area, add

Washer: indispensable for a

The following picture shows the filter wheel:

POS Filter (nm) Equalization

2 405 1 gel di 2 + 1 gel di 5

3 492 2 gel di 2 + 2 gel di 5

4 505 1 gel di 4 + 1 gel di 1

5 546 1 gel di 4 + 1 gel di 2

6 578 1 gel di 4 + 1 gel di 3