BB (LAB) 07/07/18 PRELIM 4.

Cover with Parafilm and mix

EXPERIMENT 2 5. Centrifuge for 15 secs at 3400 rpm

DEMONSTRATION OF COMMON 6. Gently dislodge the cell button

IMMUNOHEMATOLOGIC REACTION
7 Interpretations:
DEMONSTRATION OF AGGLUTINATION
Grading Clumps Free Cells Supernatant
REACTION
4+ One large None Clear/
clump colorless
A. SLIDE METHOD
3+ 2-3 Few Clear /
medium colorless
1. Prepare a glass slide label as follows:
clump
2+ Several Several pink
 Patient Name
small
 U = Unknown (Sample) 1+ Several many red
 NC = Negative Control small
NEG None All free red
2. Add 1 drop of reagent in each portion:

 U = Anti D DEMONSTRATION OF HEMOLYSIS

 NC = NSS
1. Prepare and label 2 tubes
3. Add 1 drop of Blood Sample on each portion
 P = POSITIVE
4. Mix using applicator stick  N =NEGATIVE

5. Observe the result macroscopically 2. Add 2 drops of 5% RCS

6. Interpretation: 3. Add 2 drops of reagent:

 w/in 2 mins  P = D H2O

 (+) Positive = Agglutination  N = NSS
 (-) Negative = No Agglutination
4. Cover w/ parafilm and mix
TUBE METHOD
5. Centrifuge for 15 secs at 3400 rpm
1. Prepare and label 2 tubes
6. Interpretation
 U = Undiluted
 D = Diluted  No Hemolysis = intact cell button & clear
supernatant
2. Add 2 drops of reagent  Partial Hemolysis = Presence of cell
button & pink supernatant
 U = Anti D
 Complete Hemolysis = absence of cell
 D = 1 drop anti D + 1 drop NSS
button & red supernatant
3. Add 1 drop of 5% RCS