L T P C

CODE ACOUSTICS AND OPTICAL IMAGING

3 0 0 3
COURSE OBJECTIVES
1 To learn the various principles of physics used in acoustic imaging.
2 To understand the acoustic imaging modalities.
3 To understand the the various image capturing techniques.
4 To learn the special techniques used in optical microscopy.
5 To acquire the knowledge on working principles of fluorescence imaging.
COURSE OUTCOMES
Upon completion of the course, students shall have ability to

CO1 Analyze the various principles of physics used in acoustic imaging.

CO2 Explain about the acoustic imaging modalities.
CO3 Differentiate the various image capturing techniques.
CO4 Illustrate the special techniques used in optical microscopy.
CO5 Explain about the working principles of fluorescence imaging
COURSE CONTENTS
UNIT –I PHYSICS OF ACOUSTICS 9
Hrs
The sine wave, sound in media-particle motion, propagation of sound, Speed of sound - wavelength
and frequency, complex waves harmonics, Phase, partials, octaves, spectrum, electrical, mechanical
and acoustic analogs- Wave phenomenon- Sound levels and decibel- Comparison of Logarithmic and
exponential forms, acoustic power- Measuring sound pressure level, sine wave measurement.
UNIT –II ACOUSTIC IMAGING
9Hrs
Fundamentals of photo acoustic tomography- Transducer array-based photoacoustic tomography-
Photoacoustic microscopy and computed microscopy- Photoacoustic microscopy based on acoustic
lens with variable focal length, confocal photoacoustic microscopy using a single multifunctional Lens
UNIT -III IMAGE CAPTURE
9Hrs
Optical contrasting techniques- Filter sets- Optical layout for image capture, color recording, additive
color model, subtractive color model-CCD cameras, frame-transfer array, interline-transfer array- Back
illumination, binning, capturing color, filter wheels, filter mosaics
UNIT –IV MICROSCOPY AND THREE DIMENSIONAL IMAGING
9Hrs
Nonlinear microscopy- Construction of a multiphoton microscope, fluorochromes for multiphoton
microscopy, second harmonic microscopy- High-speed confocal microscopy—Microlensarray-
Surfaces- Stereoscopy
UNIT -V FLUORESCENCE AND ITS ADVANCED TECHNIQUES 9
Hrs
Fluorescent staining- Quantitative fluorescence- Cell loading, membrane potential, fast-response dyes,
slow response dyes, fluorescence recovery after photo bleaching- Fluorescence lifetime
Microscopy(FLIM), Practical Lifetime Microscopy, frequency domain, time domain- Fluorescence
Resonant Energy Transfer (FRET), identifying and quantifying fret, increase in brightness of acceptor
emission, quenching of emission from the donor

TEXT BOOKS
1. F. Alton Everest, Ken Pohlmann, “Master Handbook of Acoustics”, McGraw-Hill,
6th edition,2014.
2. Huabei Jiang, “Photoacoustic Tomography” CRC press, Taylor & Francis Group,
1st edition, 2015.
3. Guy cox, “Optical Imaging Techniques in Cell Biology”, CRC press, Taylor &
UNIT 1- 5
Francis Group, 2nd edition, 2012.
4. Jose Luís del Cura, Pedro Seguí, Carlos Nicolau, “Learning Ultrasound Imaging”,
Springer, 1st edition 2012.
5. Peter R. Hoskins, Kevin Martin, Abigail Thrush, “Diagnostic Ultrasound: Physics
and Equipment”, Cambridge university press, 2ndedition, 2010.
REFERENCE BOOKS
1. Gerhard K. Ackermann, Jürgen Eichler, “Holography: A Practical Approach”,
WILEY-VCH Verlag GmbH & Co, 1stedition, 2008.
UNIT 1-5
2. Tuan Vo Dirh, “Biomedical photonics – Handbook”, CRC Press, 2nd edition,
2003.
3. William T. Silfvast, “Laser fundamentals”, Cambridge University Press, 2nd
edition, 2009.
WEB RESOURCES
1. https://www.ncbi.nlm.nih.gov/pubmed/15096709
2. https://www.nature.com/articles/s42005-019-0267-9

COs/POs/PSOs Mapping
Program Specific
Program Outcomes (POs)
Outcomes (PSOs)
COs
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO1 PO12 PSO1 PSO2 PSO3
1
1 - - 3 - 1 - - - - 2 - - 1 2 -
2 - - 3 - 2 - - - - 2 - - 2 2 -
3 - - 3 - 3 - - - - 2 - - 2 2 -
4 - - 3 - 3 - - - - 1 - - 2 2 -
5 - - 3 - 3 - - - - 2 - - 2 2 -