Exercise No.

2
Plant Cell (Onion Skin Wet Mount) and Animal Cell (Human Cheek Cell Wet Mount)

Names:David, Maria Iana Paula

de Leon, Mavel Kyla
Dimayacyac, Ziara Jane
Lupac, Aeron Dale
Macaraig, John Paul
Date Performed: September 15, 2016
Date Submitted: September 22, 2016 Instructor: Sir Karlo Matira

I. Objectives
The exercise aims that the students should be able to:
1. Prepare a wet mount slide of a onion and cheek cell and to be able to see
the cells that forms the certain specimen.
2. Identify the shape and cell structures that you can see under a
microscope.
3. Further differentiate the appearance of plant cell and animal cell.

II. Materials
 Onion  Lugol’s iodine stain
 Forceps  Toothpick
 Water  Cheek cell
 Glass slide  Methylene blue stain
 Cover slip

III. Methodology
A. Plant Cells (Onion Skin Wet Mount)

In doing this experiment, the delicate transparent tissue from the inner
surface of a piece of onion using forceps or tweezers must be peeled. The
tissue must be placed in a small drop of water on a glass slide unwrinkled.
Then one small drop of Lugol’s iodine stain must be added to the tissue and
must be covered with a cover slip as directed. The onion cells at low power
were ready to be examined. After the onion cells were examined at low
power, the cells at medium and high power are the next to be examined. A
diagram of onion skin tissues showing three to four cells must be prepared.
The structures that were identified from the microscope were to be labeled.
 For drawing and labeling a proper biological diagram. The guidelines to follow
must be remembered.
B. Animal Cells (Human Cheek Cell wet Mount)

In doing the next experiment, a drop of water must be placed on a clean

slide. The inside of your cheek must be gently scraped with the blunt end of a
clean toothpick and the material on the toothpick must be stirred in the drop
of water on the slide. A small drop of methylene blue stain must be added to
the slide and a coverslip as directed. The slide under low power must be
focused and examined before moving to the higher magnifications. A
diagram showing 3-4 cells of the cheek must be prepared and the structures
that were identified were to be labeled.

IV. Results and Discussion

Onion Cell under LPO (mag. 10x)

Total magnification : 100x mag.
 Onion Cell under LPO (mag. 40x)
Total magnification : 400x mag.

Cheek Cell under LPO (mag. 10x)

Total magnification : 100x mag.

Cheek Cell under LPO (mag. 40x)

Total magnification : 400x mag.

V. Questions and Answers

Part A – Plant Cell
1. Describe the shape of the cells.
 The individual cells are rectangular in shape but when look at the
cells as a whole, they are like the cubicle in a cork.
2. What cell structures and organelles can you see?
 Onion cells, cell wall, nucleus, vacuole, cytoplasm
3. How come there are no chloroplasts evident?
  It is because the onion bulb grows under the ground and it doesn’t
directly get energy from the sunlight but its green leaves that has
chloroplasts does absorb the sunlight and provides the energy to
the onion bulb under the ground.

Part B – Animal Cell

1. What are the shapes of the cell?
 Some cheek cells are round in shape but some has irregular shape
because animal cells don’t have cell wall that would give them a
definite shape.
2. What cell structures can you identify?
 Cheek cells, nucleus, cell membrane, mitochondria
3. Would the cells normally be attached to one another? Explain.
 Yes because the basic unit of life are cells and if cells are not
attached together nothing would be formed and everything would
just be a bunch small individual cells.
4. Some of the cells may be folded or wrinkled. What does this indicate to
you about thickness of the cells?
 The cells being folded or wrinkled simply means that the cells are
thin just like a sheet of paper you could easily fold or wrinkle the
paper.
5. Explain how these cells differ from the plant cells viewed previously.
 These cells doesn’t have any definite shape, some are circular in
form and some are irregular in shape unlike the plant cells that are
rectangular in shape because they have cell walls that gives all of
the plant cell same from.

VI. Conclusion
We therefore conclude that the onion do not have chromoplast due of its bulb
grows under the ground and doesn't directly get energy from the sun and
using the microscope we were able to see what onion looks like. On the other
hand the animal cell are eukaryotic cells, or cells with a membrane-bound
nucleus. animal cells also contain other membrane-bound organelles, or tiny
cellular structures, that carry out specific functions necessary for normal
cellular operation. Plant cells are eukaryotic cells that differ in several key
aspects from the cells of other eukaryotic organisms.