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NAME: Latiyah Timothy

LAB DAY: Friday


NAME OF PARTNER: Annesha Sylvester, Ranelle Stewart, Mitra Suchit , Anjanie Sharma and
Siddiiqa Seunath
DEMONSTRATOR: Sharda
DATE: Friday 18th October 2019
EXPERIMENT: # 7- Kinetics of the Inversion Of Sucrose
OBJECTIVES:
 To prepare a dilution for the use of a calibration curve
 To construct a calibration curve
 To determine molar absorptivity of a coloured complex
 To determine the concentration of phosphate in a cola drink using UV-VIS Spectroscopy.

INTRODUCTION:

Phosphorous is an element that is vital to many living organisms. It is not found in its purest
elemental form but found in phosphates. It is primarily used in detergents, fertilizers and as
phosphoric acid in cola softs drinks.
Phosphorus can be analysed in many ways. One such way is by UV-Vis spectroscopy, where the
concentration of phosphorus can be quantified. A common method used is the ascorbic acid method,
where antimony-phospho-molybdate complex and ascorbic acid react to produce a blue colour. This
blue colour can then be measured by visible spectroscopy at 830nm. The intensity of the colour is
then directly proportional to the concentration in the solution.
All spectroscopy methods are based on Beer Lambert's Law:
A=εcl
Where A: absorbance
ε: molar absorptivity (M -1
cm-1)
C: concentration (mol/L)
l : cell length (cm)

When a calibration curve is forced through the origin and the cell length is 1 cm the molar
absorptivity (ε) is equal to the slope of the calibration curve.

(Fig 1. Schematic Diagram of a Single Beam Spectrophotometer)

Due to the unselectivity of the spectrophotometer, the use of a reference sample is needed to "zero"
the spectrophotometer. This reference sample is plain, consists of distilled water and is treated the
same was the standards and unknowns. It is also used to achieve the corrected absorbance of the
solvents.

PROCEDURE
Followed as seen in the Chem 2672 Lab Manual.
RESULTS:

TABLE 1: RESULTS OBTAINED FOR THE STANDARD P SOLUTIONS

Sample Concentration Absorbance Corrected Absorbance


(mg/L) (Absorbance-Reagent Blank)

Blank 0.0 0.086 0.0

1 0.5 0.358 0.272

2 1.0 0.611 0.525

3 1.5 0.959 0.873

4 2.0 1.191 1.105

TABLE:2 RESULTS OBTAINED FOR DILUTED COLA SOLUTIONS

Samples Absorbance Corrected Cola Reagent Conc. of P in Conc. of P


Absorbance Blank Diluted Cola in Original
Corrected sample(mg/L) Cola
sample
(mg/L)

100x

Blank 0.067 0.0 0 0

1 0.987 0.92 1.65 165

2 0.989 0.922 1.65 165

3 0.975 0.908 1.63 163

200x

Blank 0.067 0.0 0 0

1 0.534 0.467 0.84 168

2 0.528 0.461 0.83 166


3 0.533 0.466 0.84 168

DATA ANALYSIS:
(1)

Graph of Corrected Absorbance (830nm) vs Concentration of Standard P


Solutions(mg/L)
1.2

f(x) = 0.56 x − 0.01


1 R² = 1
Absorbance( 830nm)

0.8

0.6

0.4

0.2

0
0 0.5 1 1.5 2 2.5
Concentration (mg/L)

Scale : X-axis: Y-axis:

(2)
Graph of Corrected Absorbance (830nm) vs Concentration of Standard P
Solutions(mol/L)
1.2

f(x) = 17306.33 x
1 R² = 1
Absorbance(830nm)

0.8

0.6

0.4

0.2

0
0 0 0 0 0 0 0 0

Concentration(mol/L)

Scale : X-axis: Y-axis:

(6) The molar absorptivity= Gradient of graph when line passes through the origin
Therefore molar absorptivity =17306 M-1 cm -1

FURTHER QUESTIONS:
1)Based on the graph, the correlation coefficient value of 0.9969 was obtained. It was more than
9900, therefore, the best fit line was good and there is near-perfect linearity between the X and Y
values.

2)The values for the [P] for 0.5ml and 1.0 ml were 167.3 mg/L±1.15mg/l and 164.3 mg/L±1.15mg/L
respectively. The values were similar, even if there was a difference of 3.5mg/L between them. The
values should be the same because the samples came from the same source.

3)From personal experiences, the pipetting aspect of the lab gave the most difficulties which caused
the most errors. This was due pipetting skills as well as the difficulty reading the values on the
graduated pipette while using it. Another error that could have been introduced is filling the
volumetric flask past its marker. These errors were seen to be problematic as well in previous labs. To
prevent these errors from happening again, I believe that proper practice with the apparatus will
suffice.

DISCUSSION:
A calibration curve was plotted and the equation of the line was used to determine the concentration
of P in the diluted cola samples of 0.5ml and 1.0ml. The concentration of P in the original samples
was found to be 164.3mg/L±1.15mg/L for 1.0ml sample and 167.3mg/L±1.15mg/L for the 0.5
sample. The concentration of P for the 0.5 ml and 1.0 ml sample were similar however the slight
differences between them are probably due errors that were introduced during the experiment.The
values also cannot be compared to the actual value of phosphoric acid in the Coca Cola because there
was no value indicated.

CONCLUSION:
In conclusion, the concentration of P in the original samples of 0.5ml and 1.0ml were determined to
be 167.3mg/L±1.15mg/L and 164.3mg/L±1.15mg/L respectively. The linear correlation value was
0.9969 and the molar absorptivity of the antimony-phospho-molybdate complex is 17306 M-1 cm -1.