[19] PEARAMEBRARP LE - (nk AS A OW OF [51] Int. cl. BOID 61/00 (2006.01 ) BOLD 63/00 (2006.01 ) * Of HIS ZL 03817994.6 > [45] BARAAA 2008 464 2A [22] IRB 2003.7.28 [21] Ing 038179946 (30) eae [32] 2002. 7.29 [33] US [31] 60/398,784 [32] 2003. 1. 9 [33] US [31] 60/438 ,784 [86] i ~—PCT/US2003/019879 2003.7. 28, [87] BaR44s W02004/011600 3 2004. 2.5 (85) ABImMne AM 2005. 1.27 (73) SARA MT BRB IIR dhe PEDAL [72] BARA C+ D+ SESH TU + HOR S +P Hi (56) Sascut 1863423898 | 2002.1.29 US6177181B 2001. 1.23 US5998588A 1999. 12.7 [54] sanz 4 [57] #8 5 OBE FB ASE DRE HE, A EE AMEN. CARN MPKMARY, Ltr US APA MEA. TE te EH LA-KHAAY, RA HERP, ATLL HoH. (11) HARASS CN 100377766C WER A [7a] erieeseaney LSA) RRA TAT LAS FORA FARE BARRA 2 HI 16 HE AHPH 15 HE03817994. 6 QA BK FH 1/258 1. AMER EAS: BURR EMAAR ATURE RBIOR OIE; Al BAAMRE TERR EARLE AOR EA. 2. BUAEOR 1 FRM, JORMECE TRS SE, ERT BARRE TAH. 3. BUNS 2 ARIA, SORRIECEF AE ARIA LIBR, SEL. Hema. WES. RRA REAR. 4. 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BRIA TR AR BE BAT HE RF AR RSE APT A» A AK A EE HD WIKRE RREA AE TH RB—-TAKEM RABE ENRAM IA, DAR JE EAHA) ATP FRG AK RE ABL/ APIA BER LMG (Soong, R.K., Bachand, G.D., Neves, H.P., Olkhovets, A.G., Craighead, H.G. #il Montemagno, C.D. (2000), Science, 290, 1555-1558 Hl) . FAFix#e esti ATP RUPEE ARE SEMA, RMR AAR EZ he A MBURPARG WR EN TRAD RXR. EARU NAHM, LARK HE AT Te S/R F, HRRAKZRES, FARMAN RBS NER. EKRGREKMAAT, WHRRAEMAM RMN MAD, CER Hak, KPBARALDHASERAOR, MBRA WR AI T AF hee REAR, AHRARAMME RAI, CANKER AB RE, FRE RRR A RR. HERA OhF lum) REM, URPRERERA HRMS, PUKKSE THES. Ak, WUHR-BRK, THERAA AEMSMMA MRE, RABE Cb F lke/o') MAR, RARE RA TRAP AIST ARM ME. RR, EO KARMA EMT RA AR/ER 503817994. 6 RWB Ra/16H FUR, ER MG RAL AE ARALEGR-BARUHEM, BRD UEARRRERE AR, AGRA AM ADE RANE MHAERA WAR, CMRI RE ABRIL EERBARKRR. ATHAHEN AMEE, BARAK TREE AR, RTM RRA RR. RABE, EMRE IES CHARNSH, FARBER. LM. NRE, EAANDARME, Wa Ba) HE A ABRAAE— AAA, Reet, CREE Pee ie eR ANARAKBA-HREA, KARO TMEAT. HARARE BAR, YEG ESS A Dy FA EE BBE, HO EB AL YT PF Sb BH A Te HFRS RRA PRE IE ED “RR”, UN RRL” LABRET WEEE. Weosh, MAP RRL REN BARE RE BS, REA AIA PARLE. RAE Hit REE EA ~ 100e/n", $20 T MRE, TORT RAT ABA RE RIAD. ARNO ART EGER SHAW EEE (>2508/ke) MARE SE (800Whr/kg 3 Ht, 9500Whr/kg 3K, 32000Whr/kg 10K) , MUTA! KERS, WARSACAMEEN 0. its, KOE AR Za, 3h Fs FFE. ARWBRS RAMA BZA RENE. AAKRA BW AM HEP NEARER A WH, CHEK. RIB. EA. WT WIRRAKE. SEE ABR KBE RINE, FL me A AE UE EY Som. EVAMEBUCMERRATCATHARRE, WMHhSEBEH Hm A, BACHNKCMAUAFH. PSORRAHAMP a DPE Ie, He AHR DD RD ORAS A SE 8 Oi aR AD ES Dh EE HA EE EZ PME) MI BNET, VEE A. A. EEA CALM BRAND, TART MEP AKEDRAB HE, MARA BE EAD Mil eS EB Pes (EBA P TAS Wt PAH HE SH RTE RE A EMRE, HARA, RP: 603817994. 6 RWB Rasex Al RARRARMM ERAN HTER, HPAP RMP ONE ME FR. FA 2 AA — AAR, HEEL AAD AT HA (Halobacteriam salinarium) F, ERGATA BR RAF IMA PH, PEER, TD ATP MRAP RAMP, AA CAAA RE KD ADP HIS ATP, FGM GRE BUA HE PE RR. FA 3A All 3b BERN COX MAHER HY, HOPE 3A EL, A 3B RAK, HT BSW=ETEREAU ART MBB “FL”. FA 4a VERA RIS ASF TB HA SC RL; FA 4b BBE HS COX $4 BAHAY HE» FAS AB As BE AO A 6 RSA eT A AT REMBRAR CV) MRSA (O) BARRE ZE 30 3h PTE RY pH BE. FA 8 VBR AI ZeZE ATP TO ACHING IIE, Hi ATP AAA ee oe BELLA AD PoP ATP A OH AER RP AE 9 HERP ALEY SEM EO CRF Inm, HA Lum) . 10 2 COX HMRRAMAL FANT Bll RUMAH NAF SPH COX MRSA RAE 12 SHE RAREA B13 2B 12 Ae A. PA 14 HRB Fa Ra A ok i a EET AK I AT. 1S RRRKPE LAR REA. ESHA RAT ERRAM-ALHARS, HARARE RARE AED HMAEREOR, SRB BRA. THRONES AMR AR, MER AZ A RT, AN MES ie REDE. RARESMES ZAR, ANSAREM NA RMD EMR. URAL (BR), (PAR ZRRAAT HERA, ROFEA 26kd 703817994. 6 BW B R5/16H HRFRZEA, MAL PRS OR, CHRARATANSMRA RR, Fe FE BA FE HR A ah KF PR AB. BR SE Hh A Ah AD AT BE RIPRPEF, SRALRBHAGETPREEN, BRARATEAL. 1H 2 BAN, BR 12 MAUR IIF 18 CA =500—-600nm) Ht, EMF 14 MHF TL ALA 16, FEMA OK. AED BR PF ERA I MER OS , BT SAT PRA, BR RAMA A BATA Sms A. i LREBREM RMA. PGA RF 14 A 12 PAR, FER TAD pH RE CIR HH) SEAM AE 16, FERS. RL ESS Day ATP QBS (ATP BE) GREET fe B, intr S 20 fia. ATP BERR 14 ASMA, PARSE ORE SBR CATP) , $0 2 RS 22 Pia. ATP AEF EMEWME, CHE DIAM MAMI. RARAMEWRAOLERE EBA fil, AAWERER DPE BR Al ATP BEA RMAAR CPitard, B., Richard, P., Dunarch, M., &# Riguard, J. (1996), Eur. J. Biochem., 235, 769-788 HL) , SEP BR ZE 40°C F AUPE Wid FAI IE EES 1. 25 OY pH Fre HE 20C, AUMH A pH=2, RAHM pH BRM ARGH, AA BR BAR FRENRGAMG. AF RRADRFHE ATP, MERA P BINT RE. ok TRIER, TARRY A aE BRA, AY OLEE AE BR 4G ATP BZ (DRA aR RRESRMNBARES, AARAR AEA, BLP EEE FARES. Mika, LUMBER, CRAM. CERHEK FRA RR” , RO PRA 7%, ARR 25% (~10 PARROT / SAM) . MRE, KAKRNRARTA 0.5m MEK. HFRLEAR PAG TE UO ET RE (OA ARSEE). CHEAT ERP R, PaAE iy F389 HS PEE T URL. LR RE TEMTEE. Heo, Beal AAT A TEMG T BRAKE. WERE IR. AIR PPR HE KOREA. ERIE Pitard Si LEAR) (150mm) AR RAP EAE BY BR, SRA, ATP DP EG BR/ AB REC CR 5 HR AAT 8B AT HY BR/ 1S RE LL RY MAB EAT OME, ACER ERE GE BR AES} GHP AE 320nmol ATP. ATP BE FUEL 2 ARE 24 Bian ADP MALALRR Ak ATP, 48 35kJ/mol AER. RAPE AMAA FE, RAR HH ATP AMARA RSE 1400/kg. 803817994. 6 BR WB Re/16H MOR ATP ABHRAFRAORES BR BEMNRAMRR-AR, UE EHF) 180—280W/kg. HP AKEARK BR REBRAMARMAR, EIR BRAKES MT PENA PAE A TAA MRT ZN BATR. CARA, RR EAR TAREE, ERK 1890 CHRAWERP, Zt pH 0-12 214, AUB E T, SSR KE, HARBIN CVsevolodov, N(1998), (EMR FOF A: HEA RMI) (Biomolecular Electronics: An Introduction via Photosensitive Proteins), 125 Rl, Birkhauser, Boston). HAAR LBRMAT, eT ATR BR PRN EAD MAH (Lorber, B. Hl DeLucas, L. J. (1990) FEBS Lett. 261, 14-18) « HY RET A BGI AE THK, WA Be Hn LA RS A Bn Stuart, J. A. ; Vought, B.W., Schmidt, E.J.; Gross, R.B.; Tallent, J.R.; Dewey, 1.6. ; Birge, R.R., IEEE EMBS, ffE'P) . AAECSAEEMMNASHLBRH, BR FT SRA WA EAL, OR (ZB) AR (PA MBEAK) (Birge, R., Gillespie, N., Izaguirre, E., Kusnetzow, A., Lawrence, A., Singh, D., Song, W., Schmidt, E., Stuart, J., Seetharaman, S., Wise, K. (1999), J. Phys. Chem. B 103, 10746-10766) . SoH ——MWERSILM (COX) RATORFREEA, HERR 46 05 0 FP BE A — A. BB 3A PRS 30 Pray COX EAH, TOR 3B PRS 32RD, TASH 34 RIL”, MART HEME. ZEOP IRD, NADH ADRES (— FP AGE) BRO. AAG XETRA E 1.0. COX MMPUR LALA ATT RET, ER c Har, A ET Ay eS FA SF OY PS Bt SHE, SK tn REE A SH cHHFER, EATHBAGRAMAAZ—-HPF OLE, KHMER AREA. ORMMGM MHS, RA SOAR RMA, RZ Fai, OPA MAR LAR. PARE FI, MIL 460mV fH Fa HE ERE #4 ff) #2 HE (Nicholls, D. (1982), (MAES: KESB Bie Fie) (Bioenergetics: An Introduction to the Chemiosmotic Theory) , 123 3, Academic Press, London) AT HAF HBEHANAS A, RESTORE LE BIG A A 1:1 (Lee, H., Das T., Rousseau, D., Mills D., Ferguson-Miller, 903817994. 6 BW B Rr/6H S., Gennis, R. (2000), Biochemistry 29, 2989-2996) , BAB Eth tk Gl (Papa, S., Lorusso, M., # Capitanio, N. (1994), J. Bioenerg. Biomembr. 26, 609-617 3, Michel, H., Behr, J., Harrenga, A., #1 Kannt, A., (1998), Ann, Rev. Biophys. Biomol. Struct. 27,329-356 HL) . bk ‘5M EAI BML (Murphy, M., AM Brand, M. (1998), Eur. J. Biochem. 173, 645-651) » YF MRA PR, PET LP RE. ATP BEBE TR FREE ATP. BR Hl COX EPERF HRA MAEM AM ARLZ MR BR A S6URB). TG COX HAL ARES SN. GRATE 2 Be, LA COX HR BRI2, DURE MER c MAMET RRR F 18, FMLA MA. SRL, BR AM COX HATAHRRAR, REBAM AM: SUA RBA AY BR A Fable RAPP, i COX MAEDA. 45 COX $E-4s A Bl RE ES BI EAR 40, I 4A A 4B RAR, PEARY BE ALFA LE RMSE FMS AS IF IZ CNaumann, R., Schmidt, E., Jonezyk, A., Fendler, K., Kadenbach, B., Liebermann, T., Offenhausser, A., Knoll (1999), Biosensors & Bioelectronics 14, 651-662 Hl) . ix#ese 5 FAL 5 BAB 44 ABE RS DRE AGAR 42 (Ey — A CME AK (DME) BA SPREE 46. HF COX PUMA ER c BA DMPE AA DH 48, Ja eH SB ARZTL, tH 4A PaRS 50 Pia. FMA, HF aBict COX $e REL Se SEMEL, VA BGR it HAI HE HF HIS BRAS SE ED ERA A A BRIBE, (IK EAE IEE RADTORRH LOR, Rave de LIN (RED IAR RARIAR. Woh, GRE A TEE POR SE EP RK, CPEREA RE, WHERE ARM WHERT ERA ESHA — HED, ANSS CAME. ERGPOCABKREWBEA GSKA, ‘EMEA SE He OF a) ERAS Dh As AS ARB DAE A HR ADR PD) AE 7 BR/COX TERT EE. EH SO BEARER DMPE fil Ay A iN PR SE HR SR. ABE YEAIMN Triton-X + AAAS ae AS B/E FREE ARE. ARRAY DRED HLAR (Pitard , 1996) , BALAZEF MEME FIERCE (Naumann 4%, 1999 #I Steinber-Yfrach, G., Rigaud, 1003817994. 6 RWB Rs/6K J., Durantini, E., Moore, A., Gust, D., Moore, T. (1998), Nature, 392, 479-482 KR). RPBARMEMHKE, TRAE AROKEK CANKER AT, ELMRERGAHA. RARKERNTOE RAMEE RS th BBN, HA CNM SOF AR ew. FEF BR AHEDL, BEAT LPL 287+) A BR #0 COX FT AE ARAYA. MERE HES FARM RL SH HARRIE PM AM THY 7.5x10", RE 4 BR AFR (25no') EA 1.9x10' 4. BR AYRIKRAMI 66000/mol/em CVsevolodov, N. (1998), (EWS FUFY: HRRAR ML) ( Biomolecular Electronics:An Introduction via Photosensitive Proteins) ,125 Hl, Birkhauser, Boston) , BRS) 4. 4x10"/MJe BR. BR AYE FRA 0.7, RFMIERE A 3. 08x10". Bulk, ATVB BR EAE PEI PARE 5.8 RMI. HE lm’ RUA ARL, BR:COX W 57:1 Mt, PHRBAHI MT MER, COX HZ AF, BRRME—TRF. CRTC, BP KBEA 3. 9x10"BR. HF COX PEN FHP BF, EGE) 37mA/m’/ BADR. 1 EE FAR RR AAD 36% R36, MELZE 76OmV 8 HSMN] 37A/m’, BS 28W/or. RELBRAREMEAT HABE, BARREN, ANG FL FS es ik 2 Th A AEA. AP A CE CD) URE 2. 3¢/n'. HF SORA MRA EA Com) 92) 105. 3g/m' MRRRRB, PENT REEL 265W/kg. ABLE 3 ADRS HOTT GAG RE 795Whr/kg, 3 RAVFLCLAE RAE 9540Whr/ke, 10 RAITT GEE HE 31800Whr/kg. HFMBRGAAM, RH HER AOE A GAR RE RE yt fal ToS KEY SAREE RT MARPRRRRM RA OHA IM, aE MEE MAN, BUMS ART RD. BARE A HER AMAL aR A, nF: (1) PRADA WE A =500—650nm TORAH): (2) SRD RRM Meat, tn RES SUAMAA, ERMA RH, BAR BR HTT. tO ERR, BRA COX MEAT HRA, CHAMBER TR F228 TB) ATP BAU ATP RF RAE. (COX RAE, BE RH HOARE COMP) » HERES RT. INGRAM ALE HEC, WERE SISAL Hi03817994. 6 BR WB R9/16I RA BRR, Ria EMF AURA. RS BR A COX MAE ARAB PNA. FOREMAN AEE 60 HRB, Hebe RAK 62 RIM 68, HRT 64 MARA WM 66. MINT HE 70 LRT, RARER CRE) 72 RE RATE. BR, PA BF 64 AY LEREME AR AY PA 74, URE RR 76 RL EAE PRA 78 EARP OLA Ft 80, PAB eR, Heme TAR MSE BB TE» BTHBARN, RARER AHWR: COX CMRRMARH, SOB 66 OAM EGFR, BRAG RMS A IRE IE i. EMF BOPADAE, BMT. RRS FTF ERA fe. BRFHARRABDANKLURAEL COX 72 RAE. RPA WSR RE ARABI, tn FoF -ATP BF (Hammes. GC. (1983), Trends Biochem. Sci. 8. 131-134) MB FHIEBA (Nicholls, D., (1982), (# Mie: KAM FI) (Bioenergetics: An Introduction to the Chemiosmotic Theory, 123 3, Academic Press, London) . ili, ARE SR UAE FAA RE EE SE eB YH Rf ZE Wikstrom ATAU (Wikstrom, (1981), Proc. Natl. Acad. Sci. USA 78, 4051-4054 7) » EMA ATP IVSRRLAR, SURES) COX pea Fash My BOA}. FoRATP BRA RRA: ATP MRT RB BAAR BE EP ATP. RL, EAT ARE ATP THE FR th. HH Wikstrom AUR, ATP RSH FAR, MMA ATP YS COX FAT. UE RAL TERR AY SP AE PLR FUR, RIZE COX LUERBBRF ED RR. BPI ATE, RRARKAE PORE, RAF MKEBA MARS, RAR OMT H. UFR ATACSRERH TR. EGER, RUMP REDARTAH PASH: Cli, SR iF, De Vault, D., (1971), Biochim. Biophys. Acta 226, 193-199 HR) : — AE=AG/(nF) LP, AERRAFHRALERBA PNRM, OC ERA H ES REEL, n RGTHRBWRE, F RAHM. EBT MAMER c OLRBHH+ 220mV) iit COX HBMMEP, WINE AE TM, BAC 1203817994. 6 ® WB R1o/18H 0, CRA BH=+ 8600) RRA HO. SHBMNT, BHRRLD 714. 8kcal/mol. JRE RATERS, APE. Wikstrom FESpHH TAMA F IRB, HE OG RM COX MERA AME, HAMAR RT TH ER ORAM ARR ES. ELA ALR”. MEK c BUH DB AR FF HB s IX LR 1.0/0. FALL oe SB A G AE TT RoHS ET AG ROMA RE, BRERA, WKS FF thes COX. AM, TMCS ARB HRA ERM, AA MRAP HE. EARRWAA, OE 5 AIA, COX BERRA Wikstrom MRAM HF HA-—-HMER co BRARBMBRFEA——O. AARP RE BE 78 it AREA, HO BY 820mV EK, MFR MARIS. AP RAMA HK a EM, SHEALERBH+ 3800, MARTA ROMO. BMA a BY, oe FES PRET 140 B+240mV, AREF MARE. BI) Cunt, Haat — HF BE 50 Bl + 190mV, MSS ZE OV BBA 80 Lb, BRAKE. BT FARMER 78 HSH MAR a LARA CARE RM) . IRMA BIBRA. AFM 03 HBB RRL FE 380m, TERESA AEE A CRF AA) ARE RA. AA BR 62 FEA 66 HATA MBA, WARE A Fe HFRRARF EA, MEMRE RFI HO RBA, i AIRE FOZ 18 AY CE fT EP Ud RABROHLHATH RA, HABLA RAENRARAOEK, RR CE AS BR ots BEE A ie TB BR ACT TH AE (Pitard , 1996) . AREAL, INR 40 (A 4A) RE SEMHAERAWERMEA-RASEAR, AE ARF RMR. RERAWE AAA, REK (a) CNR EAR ALP HH, (b) EMEMEARUT AA ROAR, ASA RGBA RA UAL, Cc) BELTS BBY BES Oy BAB REE EA RRA REG, DIAMAR MEA RAN ARURE. RRR MRA WAAR FERRIER, CAA RAI ALB AREA ARIE. BR 62 #1 COX 72 ERA WR 66 PEA, BLA HR 78 #80 Am. 2 ARAKI ATPEP ARAM. MTLEMIPE, BARTER Ay PI-ATP ABSA MA KLEE. MAS Kim, ARMGRAD BR Fl ATP BAO AE BAR if 66 RUMI, ATP ARLE MAKE AL, BN ALS RIE 1303817994. 6 ® WB Ru/eH AY RERIESE A ADP FH: ATP, BABU T-BAR, AFAR EY Moth RAR (BR), MP RTP MR, ABER CA 6p Hz 90) » BARU, FASC MOR REAR, BERRA, AAAI (Pitard 33, 1996) BARRA. AERA 0. 45 #10. 2n mite RRERT, He HEP FIER -F 200nm. FoF.-ATP SEAN BR AYIGAZE Triton X-100 ATF EPH. WARIEM MAMIE, MMA Pyranine (% pH BURIEARA, dea UA ARG ESL ARP. LR, KB 1.5 AY pH PREATZE 20 ‘CRA, WA 7 PH 92 94 Aim, PMB RAM (the 92) MAAR AUR CHAR 94) UAB PR ZE 30 SP EP AE RAY pH BREE. STE, FARRAH ATP, MOP 8 PAKS 96 Kia. WAT BT TERE BPO AE BY ATP TORE AN RIE H FEI, AA LA I EAN BR 7 FoF\-ATP BE. ADP MARR AT, ABR ORTEIEIS POR SR 2.5 DRT. APR OR LE BABE, (EARAS ATP AP EH OB AT TRAE (Pitard 4, 1996) . APART BRN ZO Bl EI “KEN” —— HERA KR RAD F BER BSP. ULAR RR KH SH EE ck. FLO RGERPES 100 MBO EMA, CMBR) F 10nm HR 102 ME 4245 1 m AYHH 104. FAIR ERED 100 HE AR, FAG AHA RAK AD PED, PED APD FBR EUR . REP ED th ft UF IRE Aaa TAR OK AK BBA BR AEF ANE RAL A ARAY. ARAF ER UA SOL HABE, MEARE ANSE LZ Ia, 5) 100mg WE MRM. RSM Or MEARARMRARA, CAUMAR AUSF. ISAM Zhen, Y., Qian, J., Follmann, k., Heyward, T., Nilsson, T., Dahn, M., Hilmi, , Hamer, A., Hosler, J., Ferguson-Miller, S. (1998), Prot. Expr. & Pur. 13, 326-336 STB +EF* COX (Zhen, 1998) . we A 32 He eo SER AT SHB (Rhodobacter sphaeroides) MRiAMAL COX, MFA 10 be 90 ta. PRAIA PORE pRK-pYJ123H Mite LH RA HER c FUL AGA WALT 1 AEE (coxl) WIEKEA PUCLO, FIFI pJS2-K6H2 AY Smal fk, PAE pIS3-SH, WAAMTAR. MiG His-tag MAN-HABPILT cox] fY C-3H, WEILER pYJ100 AY Pst 1/Pstl H BRERA pJS3-SH b AVE “403817994. 6 WB Miz/6H PstI (ARPA pYJ124H. HA, FA EcoRI Al Hind] MARES RE FA RIA BAB Prk4l5-1 P. UeAEP Ee 6lmg/10L HFM, ANT AMBRE HAMA, KR-TANE. PAE AD we BR ALS A BR BAA RP AE HN. FE BO ARAZLH, BRACARRAD REBAR TOMER, BRR AOA HMA G. All Pies 120 PARSE PE A et, ARH ARM SRF RHR EMH, BAT PRR. Itoh, A WEST ER/BARA AY Lin sh. veh LIER F 121, Ag:AgC] (HI KCL SAAR 122, MARU 123, PPE 124, BRA 125, HARE A 126 AAR BAR 127, ff Naumann frie. ATFRBM RNA, LRKPRETHSRA: PHR-BMR, BH JWGRIN Langmuir-Blodgett MUA; CRAMER T BRA, ER FELARMM BH, MAHAR AA. SHRARAA PM MAKWKRA ARIE (HRA lum BAN ~ 10° BH) . RAHA AB BR (AINE OMB Git 570 BARMERA 55 BH). ABM EH BIN ~20V/cm fr SHRUG BR EL, AAD. Be trH COX MBRBR RBA, VIER c HR. COX ASE PAA A IL S| AE FE A, BEAR BR AB RE. FN COX ft RAR EB PER Ok i, BMAP REAM. MABE AHIR Mia. Blk, A SEtE COX SAME ARP Z ATER RE IA]. COX ARERR MA PM, SE Pe SRUGN , RSE COX MAE. WFR COX HOHE Ay EES Sab HY FRM AAA, GONE AR. BR KAKA, RB SB, JERE IN AY Ae ERGEREKY, BRACE BH, LARHBLRHM AMS. LIK BE AS RR PE PM A COX 5 AS th A Re BR PR FS i3e A Fa Fi et COX AY HIE RAB A SS AP FE FA] BR AUB See FE ERPS 8 AS HEAT A. RF REF ARSE AM Nes. FA COX BMH. EAS MARSH, WR 78 A 80 MARRS MRT, te PAREN ABER c A OME. AAR COX 72 RMT AE TRF HAY se IR, TT HRA T ROLF WTB A A BR BY SESH TAB pH BRIG AH BLS COX HE HR BA BE 77 A LH EE Pe 1503817994. 6 WB Ris/8H FARES (Naumann 4%, 1999) AIA ATIRMEE BR AFR, SET A HS BE BAR COX RAF KE AA Ie ET FE TL ALCOA BY «LA PH 5] SPF EE PE RO ATP BGT IB ALA COX RARER. EAL COX PEF RARE, RUS ATP SEPT ARS & ATP. BR FLAT Sat ATP, KARE LA —-SER BRE, MPH ATP ROW AKRT AGRE EX. FAURE THE, PET —T ATL pH BEBE, BIR ERS a] — (i) ORE AFA. AMER MEME COX ZAR, WERT MARE HD FLFR AEE COX MARY. DURATION 11 Bi, MPR. ERMA, TERRE RRM RE. TRA S Hie RS BA Ba. AG RL ED TL a, KUKARZM, WERT EEN. MMR LRLM KR, HECRLRE RANE BM, BRR. Re, Bb O Prana San SAS AF LA Pe AR eT LT ART ZEA Te CRELBABAKR. BRAM ER, BEAM COX A BR ALA TERE TURP BR Al COX (EAA A MEM AT: PTT EA. MRE FAT, in — +S RA HE. MRE ARTE, RED KAY PM SR BAR IE . PERE RL HE COX BSE, RRA LE—7P LH PM FL, PM RA LD, LURK FI COX, AM RAI HERIE, PM HELICAL RET SAHA. HA), WEN BR AU, HART ATR. RA Bi EBS AS AR Lit Yh SV. BAL AT eA, HBL RDP PABA it BT WA BEE BB 4) ae TE FAAS COX FEI AARF BT COX RB, ALARA BR AEM RFR, i WY. JERR BE ARTE be « AS FB Dit th Ae PE FAY COX 4) F ScAMRIE He, ea FARRER COX DFR THR. MEAT, PP EMRE, >200mV, PED 7 AG HR BE A DA GE 24 Hy ALF 1 ETP RE COX PTT. EDD EASA MAIER CEM COX HEA GS SHEE BS TESA REET EER, NER REMIRE TEER, Fl03817994. 6 WB Rasim BRARSOR, BHM: CHKERRAARKN AH, CHNRMe A: ENAEAAMSMER, MRT HAF SRE RMB. RRA DRG AA EE A, DEBT DURST RR RIE RR IN EG RHE. WSLDMSEMRA AT ZMER, WRI. RH, REM T Sate. Se. RAS RA AMBER RADYASEA AMARA. UR FOREARM. RAWREMSREDRRER, RACER SE EAR ESET PRE BAG. RAWNAGUREMRERS EARN AAU te AACE, ERE PRIR MOT A. PR AG A A AE AEB ROT PY FAP PRL WET BAAS le. APR, RARER EE ASHP SRK, AIR TAT HEA RA PE AT Th. EDR) AP BS AY Ha EA AK A-AH, BULA KMHRAREAREAHRARROR, FPETR SO vE RAR EAR, LEK TL, BRAG TAT TRE. 7K HR AMOS RHE, CIR. R. . BER, DNA dy Yeh), BRAK, RERAKBROAF, KBR AS TARA HAE HZ. MI 12 HAR, ENKI RA 130 AA 6 PEER o He RIK 132 137, ENTE A RE ECE REP , AURA REE ORE AMY NPA FE 138 7 140, El] LEARPOGAADARRE-K, CRA ROR. WORM ELE ADFT, OU 13 Pas 142 Him. BHA, KBE HH, AEA APT: K-RLABEN, AAKMATNRRER. 7k DURE 52 i Ait ED 7k A B.S 14 am. RA AR BLP] FL SESS AE 150 SEI, VBE 152 A 154, PRARAITERR 156 PAT, RPGR T ABBA. KARA, CHB 152 PMTs 158 MB 154 PASE 7K 160 IF. RAM KMBEAT SZ. Ast, SHR—WL MARA ISK 158 BEANE A PR, Sik RRB RHEE A — 154. Fcc, SEAT KAAGRG, Ro, MRARPSARNAHLED A, UAT GE MREK, BRM RRELSORAEMART. GRENR, AKECEGHBATARE, RRARRCMKAS HE. HERA ARI VFS RA COKE 2 (AqpZ) ) EMRE, WAR SKRPNBRS 4, MARRAW AE. Aqp2 TER, ER, PERT M IEE FRA REM . 703817994. 6 ® WB M15/16H GEM, BEBE AE RY FE YP Cy PG BR RU, Ky HT FE BB, ‘CEA THR aR Aqp? thE RISE A. ea Fick Be A ee EPA EB BS — th eI, KERR NBME BACRA RE, HURRARADMRERA RS, WE AREA RITE. Alt, WHR RTL, DUR RATRMA, eae FARRAR IT RA URORAI AE. GUNN, fa PA 13 oP SRE RIB 142 ONFERERRER MEMRAM, HPSS RE SE LE PIER, Ae FRAT TG PP eS 7k. Fle, ABER EHR BT AY Ik Se ee AT KEPPRA, BARWRNRELAARN, 7K i BME bia). ARAVKAELMARRKEAH CL, AACRABM EGE. Ail XH, WA Langmuir-Blodgett #7 25mm AWM MRM LAK Sam BMBRAMHERARRO NEAR. RRA 254nm WRAL, ERGMRR, MMS. i, ARM HALE A 220nm BY PVDF HR PRR L, UMRRS RIEHL WRMIR. HES, HEASEME 4 RSA, AMEKRFTR. AR ARI — AS EP, UK FE. BUMS HAY LIER, CA SEAR Bk Ba A BK LB PKL EA CRM ANARERBE, ARK REA PRANK. RM ABA, RARE TAB/ VARY (psi) RH. Hn, MKHEBRAA 360psie AGT AEA ARERR ERM. BRERA CHAR SWRRS, HAMA UW HARK, VRKBAE. H-AHERERKEP DASKEMAS. ARENER, URENBEFUR, Meth Sie, BREBCE. RA, MDRSATAEANARDULES AS ROR KK KRSM BE MEN EES Aqpd Bf. HARARE SE RRHEN, CREREARRRENN— MD. Aik, SPHRABEDEN, RATER TT REEIRA. Alt, WRENSZMMREEAR 10%, MRE 100%, Ai 10% M4) LRA RED PLE TERK. BSP a FEW Hy Fie EEF AE Hh Ik TRGB IEE RBH FG — BO BT 1 A ILA RS BS YK. SO 15 PERS 170 MER GMRRABMT AD, LKR 172, Bit 1803817994. 6 ® WB M16/16H WER. AF ACH, UV LSE RB 174 MM RAMESH 176 EEE TK CBI SCA 7K SEL Aa Sa AG 7k A EGA ERT) ZT A B S ER KEE ADE KEP REA RD F 150 I AE ES, FAIRE HE. UV AE AA. PALS TT a BSH. CHAE. RARBEARENDAER. Bie, SRS ASA, MREMRSADES, BRAS, RMARAR AMIE HSAPKSE ETM REI EAR CLR AR. EIB CR.O.) BATES} BP REPS EH 28.4 Ft (7.5 Me) rk. MATAR, Aka F iit AapZ HAE ALCO. 017 7mm*) AB XH 544 mol/s(Pohl, P., Saparov, $.M., Borgnia, M. J., Hl Agre. P. (2001) Proceedings of the National Academy of Science 98, 9624-9629 HR) . Alt, MB 1.0 FARA ABBA 2 RSA Hews 3295 FARK. PEER LE LIP AK AER 116 f= a, £PRMEFRARORE RR. AKG LER PA DE BIR RMT KO —Aapl. MAMI PF LEE) 2. Sng AEE AUR. AMY 5 RICHER REP OEE 10mg RAR. MTL ART SHERBORAR. QM Aap. HRAWTEA-LRSPHA, Bh RCE RAR ILA RD. KE BA 2 RSRRRHM. AR. Her HA PUI. Bb, CAAT AEA AK, ah aK RSE Ah Ps RM a PD RE FREAK AEMRS. RRR T BA KEE EA AMS 4, SEHR HT REA AUER AE PKG UE 7k BB AY iH. BRLMAA DELMAR AR (ET, (AR, TDR TR FEMBHMTSHRANEK, RERARARAMMP MEE, ML FL MERB AB.03817994. 6 ii of MM al E (aR RB) _f 20RoW BM 218K 4g | (H4] 2 KABAWo M B/t5 NE Be 7994.6 0381 RABAK 2203817994. 6 Row Rav isme . 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