MORPHOLOGY TOXINS/ANTIGENS PATHOGENICITY CULTURE AND LAB DIAGNOSIS TREATMENT/

MICROSCOPY PROPHYALXIS
CLOSTRIDIUM PERFRINGES
SPORES - OVAL, α - LECITHINASE Gas gangrene, BLOOD AGAR – NAGLER SURGICAL
SUBTERMINAL, CLUB Ө – CYTOLYSIN Food Poisoning, TARGET REACTION - +ve, REMOVAL OF
SHAPED (STREPTOLYSIN) Gangrenous HEMOLYSIS. ROBERTSON DEBRIS
CAPSULATED Ꜫ - EDEMA appendicitis, pH – 5.5-8 COOKED MEDIA HYPERBARIC 02,
BOILING LESS THAN µ- Necrotising Temp. - 20-50 C. – PINK COLOUR ANTIBIOTICS –
5 MIN HYALURONIDASE enteritis, GRAM STAIN - (SACCROLYTIC), METRONIDAZOL
TYPE A HRS ν – DNase. Biliary tract +ve BACILLI, REVERSE CAMP E,
TYPE C 1-2 HRS infection, MOTILE TEST - +ve CEPHALOSPORI
SIZE – 4-6 X 1 µm. Panopthamitis, -PERITRICHOUS, LITMUS TEST – N, CLINDAMICIN
Thoracic Infection, SINGLE/CHAINS. STORMY CLOT PASSIVE
Urogenital FORMATION. IMMUNISATION
Infection. VP -ve, :
INDOLE -ve, ANTI GAS
MR +ve, GANGRENE
H2S SERUM – 10,000
PRODUCTION, U (PERFRINGES,
NITRATE NOVYI),
REDUCTION. 5000 U
(SEPTICUM).
CLOSTRIDIUM TETANI
SPORES – SPHERICAL TETANOLYSIN Tetanus (Tonic Temp – 37 C BIOCHEMICAL SURGICAL
AND TERMINAL, (HEMOLYSIN) – Muscular Spasm) pH – 7.4 REACTION: REMOVAL OF
BOILING – 15-90 OXYGEN LABILE, Etiology – Infection SACHROLYTIC, DEBRIS,
MIN, HEAT STABLE. of wound. ORDINARY INDOLE +ve, ANTIBIOTICS
DRUM STICK Incubation – 6-12 MEDIA, VR -ve, METRONIDAZOL
APPEAREANCE, TETANOSPASMIN days, BLOOD AGAR– MP -ve, E, PENICILLIN,
NON- (NEUROTOXIN) – MOA – SWARMING ON H2S NOT BACITRACIN,
ENCAPSULATED, OXYGEN STABLE, Retrograde axonal INCUBATION PRODUCED, NEOMYCIN
OBLIGATE HEAT LABILE. transport to cell FOR 1-2 DAYS, NITRATE NOT APPLIED
ANAEROBES. bodies of neuron in POLYMIXIN REDUCED. LOCALLY.
brain stem and ENHANCES IMMUNISATION
spinal cord. SELECTION, TOXICIGENICITY PASSIVE
Inhibit the release IN VITRO: – ATS(HORSE)
of inhibitory FILDES ANTITOXIN ON HIG(HUMAN)
neurotransmitter TECHNIQUE, ONE HALF ACTIVE
(GABA, Glycine), DEEP AGAR SHAKE INHIBIT PLAIN TOXOID
Degrade CULTURE, HEMOLYSIS, ADSORBED
Synaptobrevin. GELATIN STAB IN VIVO: – TOXOID
CULTURE (FIR INOCULATION TRIPLE
TREE), IN TAIL OF VACCINE ALONG
MOUSE TO TEST WITH
ROBERTSON RIGIDITY. DIPTHERIA,
COOKED MEDIA PERTUSIS.
- BLACK COLOUR COMBINED
(PROTEOLYTIC). TOXOID+HIG.
CLOSTRIDIUM BOTULISM
SPORES – OVAL, SUB EXOTOXINS: BOTULISM ORDINARY TOXIN IN FOOD PROPER
TERMINAL A – FOOD M.O.A – Inhibit the MEDIA – AND FECES. CANNING AND
BULGING SPORES B – FOOD release of Ach in Temp. – 35 C PRESERVATION,
100 C FOR SEVERAL C – BIRDS motor neuron at LARGE, TOXIGENECITY ANTI TOXIN IM
HRS D – MAMMALS synapse. Leading IRREGULAR, TESTING FOR
NON- E – FISH to paralysis. SEMI- ANIMAL POISIONING.
ENCAPSULATED F TRANSPARENT INOCULATION
8 SEROTYPES G – ASSOCIATED Types: AND FIMBRIATE INTRA- ACTIVE
A, B, C, C2, D, E, F, G WIH SUDDEN 1. Food Botulism, BORDER. PERITONEALLY IMMUNISATION
NON ENVASIVE. DEATH. 2. Wound – POLYVALENT
Botulism, IN ALKALINE ANTISERUM A,
RELEASED ON 3. Infant GLUCOSE B, E TWO INJ. 10
AUTOLYSIS Botulism. GELATIN MEDIA WEEKS
SYNTHESISED AS – IT PRODUCES INTERVAL AND
PROTOXIN, SPORES A BOOSTER
ACTIVATED BY CONSISTENTLY. YEAR LATER.
TRYPSIN
A, C, E – DEGRADE SUPPORTIVE
SNAP 25 THERAPY.
C – SYNTAXIN
REST –
SYNAPTOBREVIN.

MYCOBACTERIUM TUBERCULOSIS
STRAIGHT/SLIGHTLY LIPIDS – MYCOLIC TUBERCULOSIS SOLID MEDIA – NIACIN TEST – ANTIBIOTICS
CURVED ROD, ACID(ANTIGENIC) LJ MEDIA, +ve CANARY BACTERIOCIDAL
SINGLE/PAIRS/SMAL PRIMARY EGG – YELLOW H – ISONIAZID
L CLUMPS. PROTIEN – INDUCE TUBERCULOSIS – PETRAGNINI, COLOUR. R – RIFAMPICIN
SIZE - 3µm X 0.7µm TUBERCULIN Acute DORSET. ARYL SULFATE Z–
REACTION. (INDUCE Inflammation with BLOOD – TEST – PYRAZINAMIDE
LONG, DELAYED TYPE edema fluid, TARSHIS +ve PINK S–
FILAMENTOUS, CLUB HYPERSENSITIVITY) polymorphonuclear SERUM – COLOUR. STREPTOMYCIN
SHAPED AND . leucocytes. LOEFLER CATALASE AND
BRANCHING FORMS. Later Monocyte POTATO – PEROXIDASE BACTERIO
POLYSACCHARIDE – surround bacilli, PAWLOWSKY TEST – STATIC
(INDUCE resemble Bacterial EFFERVESCENE E–
IMMEDIATE TYPE Pneumonia. LIQUID MEDIA (CATALASE ETHAMBUTOL
HYPERSENTIVITY). Heals by DUBO’S, POSITIVE)
Resolution. MIDDLEBROOKS, BROWNING DOSAGE
PROSKAUER, (PEROXIDASE HRZE – INITIAL 2
SECONDARY BECK’S, POSITIVE) MONTHS AND
TUBERCULOSIS – SULA & SULTAN.
Recurrent infection DIAGNOSIS : HR FOR NEXT 4
Granuloma is AUTOMATED MONTHS
present METHODS – NITRATE RED. – FOR 3 TIMES A
Central layer – BACTEC 460 +ve RED WEEK.
Multinuclear Giant BACTEC ALERT COLOUR
Cells. BACTEC MGIT AMIDASE TEST
ESP SYSTEM. – +ve
CONCENTRATION OF Middle layer – MICROSCOPY PYRAZINAMIDE EXTRA
SPECIMEN Epithelioid cells. ZN STAIN – AFB TEST – +ve PULMONARY
PETROFF METHOD Peripheral Layers – NEUTRAL RED –
SPUTUM + Eq. VOL Fibroblast, KINYOUN’S VIRULENT TB MENINGITIS
4% SOD. HYDROXIDE Lymphoblast, MODIFICATION STRAIN BIND – CSF DEVELOPS
+CENTRIFUGE Monocyte. OF AFS – BUT NOT WEB CLOTS.
3000RPM Decolourisation AVIRULENT.
NEUTRALISED BY by 90% Ethanol TWEEN 80 MILIARY TB –
N/10 HCL and 5% Hcl and HYDROLYSIS – BONE MARROW
USED FOR SMEAR, Counterstained CLOUR AND LIVER
CULTURE AND by Loeffler CHANGES FROM BIOPSY.
INOCULATION. Methylene Blue YELLOW TO RED
(Bright Red Rod). AT pH 7. RENAL TB –
Acid and Alcohol URINARY
Fast. TUBERCULIN SECRETION.
TEST
AURAMINE 0.5ml PPD
RHODAMINE 48-72 HRS
FLOURESCENE +ve ¿10 mm
Auramine phenol -ve ¿5mm
or auramine Equivocal - 6-9
rhodamine mm
fluorescent dye
is used and INF GAMMA
examined under ASSAY –
microscope. DETECTS INF
GAMMA
MOLECULAR RELEASED BY
METHODS: CD4+T CELLS.

PCR – EXTRA ANIMAL

PULMONARY TB INOCULATION
12 WEEKS OLD
TMA – GUINEA PIG,
TRANSCRIPTION INOCULATED IM
MEDIATED IN THIGH.
AMPLIFICATION. PIG DIE AFTER 4
WEEKS –
RFLP & FINGER AUTOPSY TO
PRINTING. FIND M. TB IF
NOT THEN
LPA – LINE AGAIN DONE AT
PROBE ASSAY. 8 WEEKS.